MD12G1254300.v1.1

Encoded by

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0001068
Physical Location & Seq
Forward (+)
32271325 .. 32274408
3084 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1254300.v1.1.491

Sequence Viewer

Length: 1218 bp
ATGGCTTACGTTCCACCACACAGGCGGCACTCGAAGGAACTGGAGAGGCCATTGGTCACTCCAGAGCTGCTTGCTCCTCAATTCAAGAAAAACTTAAAAGTCAAGCCATTTAATAAAGTGGATGAAAATATAGTTTATGCAGACCATTCTACACATGGATGGTGCGCCATCCCTTTGGATGACGAGAACCAGTTCCCATCTTCTGTTAATCTTGAGCCCGTTACCTTGGAGTCTGTTGAGCTGAATTTTGGAGAAAATCATCTAGCTTTGATCAATACTAGTCTAGATAATGGCAGTGAGGTGAAATGGAACTTGCCAAGGAGCCCTTGGGAATCTATAACAGAAAATGTGCTGGAAGACTTACTTTCGGCATTTAAACACGCGAGGAATGAAATGAAGTCTGCAAAGCCAAAAGAAGCATATCCAACTTTGATTGCCAGAGTGGGGAAAGTACTCTTTCGCAGGAACCCTTCAGTTAACTTGGAATCCATTAGAAAAAATTTGTTCACTGAAATCCCGAAACGACGGAGGCAATTATTTTACACGAACACTCCTGTTTCGTATAAGGAAAAGATTGTGAACAAAGTTGTCCCGAAAATTGGAGTTGTTTTCGAAGAGGAGGAAGATATATACGATGTACAGTTGTCTGATTCTACGAGTCCAGAGTCCACTCTCTTCTGCAGATGTCGGGTAATGAAAGAACATGGAAGGCTACAACTCTGTGAGATCAAATTGAAACCAGTGCGTAACATGGTAAAGGACATTTCATGCACTACTAAGAATCTGGACCTGAGACTAATGCTGTGCACAAAGAAACTCGTAACTGATCTGACTAATGATGAGATGCAAAGCATCACGGATCTGATTAATTCTGCGATTCTAGATCCAGATGTGAAGGGTGGATTGAGGTGGCCCCCGGGGAAGGAGTCTTCAGGAGAAAAATACACAGTTGTTAGGTTTACGCACGTAATAGTTAATACGTATAGAAATTCATCACTGAGACTGAAAGTAAAACACTATGATCGATTTGATTTGAGAACCTTAACTGGGGAAGCTTCTTGGGGGACAAGTCTGGTGCTGGAAAACGTCGTGTCAAAGTTACAGGAAGAGAATGTCAAGGCCAGCACAATTTCTGAGATACTCAAGGAAGATATGCAGTTGATATGGGAAAACTTCTTGAGCTGTGAACCTTTTCTATCAAGAAATTGCATGTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

406

Amino Acids

46.59

Weight (kDa)

8.01

Isoelectric Point (pI)

48.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7903 PF25475 42 - 392 4.5e-144 Domain of unknown function (DUF7903)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 383
AciI CCGC 1 cut(s) 25
AclWI GGATC 2 cut(s) 867, 878
AcsI RAATTY 3 cut(s) 244, 499, 988
AcuI CTGAAG 2 cut(s) 456, 915
AfaI GTAC 2 cut(s) 453, 639
AfiI CCNNNNNNNGG 4 cut(s) 444, 599, 922, 1047
AgsI TTSAA 2 cut(s) 85, 736
AhlI ACTAGT 1 cut(s) 278
AluBI AGCT 5 cut(s) 67, 241, 266, 1055, 1182
AluI AGCT 5 cut(s) 67, 241, 266, 1055, 1182
Alw21I GWGCWC 1 cut(s) 809
Alw26I GTCTC 2 cut(s) 787, 994
Alw44I GTGCAC 1 cut(s) 805
AlwI GGATC 2 cut(s) 867, 878
Ama87I CYCGRG 1 cut(s) 916
AoxI GGCC 3 cut(s) 47, 911, 1119
ApaLI GTGCAC 1 cut(s) 805
ApeKI GCWGC 1 cut(s) 67
ApoI RAATTY 3 cut(s) 244, 499, 988
AseI ATTAAT 1 cut(s) 867
Asp700I GAANNNNTTC 2 cut(s) 191, 1191
AspLEI GCGC 1 cut(s) 167
AspS9I GGNCC 2 cut(s) 787, 912
AsuC2I CCSGG 2 cut(s) 917, 918
AsuHPI GGTGA 1 cut(s) 313
AsuII TTCGAA 1 cut(s) 612
AvaI CYCGRG 1 cut(s) 916
AvaII GGWCC 1 cut(s) 787
BaeGI GKGCMC 1 cut(s) 809
BanII GRGCYC 2 cut(s) 219, 326
BbsI GAAGAC 2 cut(s) 363, 921
Bbv12I GWGCWC 1 cut(s) 809
BbvI GCAGC 1 cut(s) 54
BccI CCATC 3 cut(s) 153, 176, 205
BclI TGATCA 1 cut(s) 270
BcnI CCSGG 2 cut(s) 917, 918
BcoDI GTCTC 2 cut(s) 787, 994
BcuI ACTAGT 1 cut(s) 278
BfaI CTAG 4 cut(s) 263, 279, 284, 881
BfmI CTRYAG 1 cut(s) 679
BisI GCNGC 2 cut(s) 26, 68
BlsI GCNGC 2 cut(s) 27, 69
BmcAI AGTACT 1 cut(s) 453
Bme1390I CCNGG 2 cut(s) 917, 918
Bme18I GGWCC 1 cut(s) 787
BmeT110I CYCGRG 1 cut(s) 916
BmgT120I GGNCC 2 cut(s) 787, 912
BmiI GGNNCC 3 cut(s) 323, 467, 914
BmrFI CCNGG 2 cut(s) 917, 918
BmrI ACTGGG 1 cut(s) 1056
BmsI GCATC 2 cut(s) 834, 861
BmuI ACTGGG 1 cut(s) 1056
BpiI GAAGAC 2 cut(s) 363, 921
BpmI CTGGAG 2 cut(s) 45, 62
Bpu14I TTCGAA 1 cut(s) 612
BpuEI CTTGAG 3 cut(s) 233, 1127, 1198
BpuMI CCSGG 2 cut(s) 917, 918
Bsa29I ATCGAT 1 cut(s) 1024
BsaAI YACGTR 2 cut(s) 967, 981
BsaJI CCNNGG 6 cut(s) 225, 317, 326, 915, 916, 917
BsaXI ACNNNNNCTCC 2 cut(s) 535, 565
Bsc4I CCNNNNNNNGG 4 cut(s) 444, 599, 922, 1047
Bse1I ACTGG 4 cut(s) 45, 190, 740, 1051
BseCI ATCGAT 1 cut(s) 1024
BseDI CCNNGG 6 cut(s) 225, 317, 326, 915, 916, 917
BseGI GGATG 4 cut(s) 127, 164, 168, 184
BseLI CCNNNNNNNGG 4 cut(s) 444, 599, 922, 1047
BseMII CTCAG 3 cut(s) 782, 989, 1125
BseNI ACTGG 4 cut(s) 45, 190, 740, 1051
BseRI GAGGAG 2 cut(s) 66, 632
BseSI GKGCMC 1 cut(s) 809
BseXI GCAGC 1 cut(s) 54
Bsh1236I CGCG 1 cut(s) 383
BshFI GGCC 3 cut(s) 49, 913, 1121
BshVI ATCGAT 1 cut(s) 1024
BsiHKAI GWGCWC 1 cut(s) 809
BsiHKCI CYCGRG 1 cut(s) 916
BsiSI CCGG 1 cut(s) 917
BslFI GGGAC 2 cut(s) 575, 1078
BslI CCNNNNNNNGG 4 cut(s) 444, 599, 922, 1047
BsmAI GTCTC 2 cut(s) 787, 994
BsmFI GGGAC 2 cut(s) 575, 1078
BsnI GGCC 3 cut(s) 49, 913, 1121
BsoBI CYCGRG 1 cut(s) 916
Bsp119I TTCGAA 1 cut(s) 612
Bsp1286I GDGCHC 3 cut(s) 219, 326, 809
Bsp1407I TGTACA 1 cut(s) 637
Bsp143I GATC 6 cut(s) 270, 726, 826, 859, 883, 1021
BspACI CCGC 1 cut(s) 25
BspANI GGCC 3 cut(s) 49, 913, 1121
BspCNI CTCAG 3 cut(s) 783, 990, 1126
BspDI ATCGAT 1 cut(s) 1024
BspFNI CGCG 1 cut(s) 383
BspLI GGNNCC 3 cut(s) 323, 467, 914
BspMAI CTGCAG 1 cut(s) 683
BspPI GGATC 2 cut(s) 867, 878
BspT104I TTCGAA 1 cut(s) 612
BsrGI TGTACA 1 cut(s) 637
BsrI ACTGG 4 cut(s) 45, 190, 740, 1051
BssECI CCNNGG 6 cut(s) 225, 317, 326, 915, 916, 917
BssMI GATC 6 cut(s) 270, 726, 826, 859, 883, 1021
BssT1I CCWWGG 3 cut(s) 225, 317, 326
Bst4CI ACNGT 2 cut(s) 642, 949
Bst6I CTCTTC 3 cut(s) 609, 680, 1101
BstAUI TGTACA 1 cut(s) 637
BstBAI YACGTR 2 cut(s) 967, 981
BstBI TTCGAA 1 cut(s) 612
BstC8I GCNNGC 2 cut(s) 72, 1123
BstDEI CTNAG 4 cut(s) 777, 791, 998, 1134
BstF5I GGATG 4 cut(s) 127, 164, 168, 184
BstFNI CGCG 1 cut(s) 383
BstHHI GCGC 1 cut(s) 167
BstKTI GATC 6 cut(s) 273, 729, 829, 862, 886, 1024
BstMAI GTCTC 2 cut(s) 787, 994
BstMBI GATC 6 cut(s) 270, 726, 826, 859, 883, 1021
BstNSI RCATGY 1 cut(s) 1213
BstSCI CCNGG 2 cut(s) 915, 916
BstSFI CTRYAG 1 cut(s) 679
BstSLI GKGCMC 1 cut(s) 809
BstSNI TACGTA 1 cut(s) 981
BstUI CGCG 1 cut(s) 383
BstV1I GCAGC 1 cut(s) 54
BstV2I GAAGAC 2 cut(s) 363, 921
BstX2I RGATCY 2 cut(s) 859, 883
BstXI CCANNNNNNTGG 1 cut(s) 175
BstYI RGATCY 2 cut(s) 859, 883
Bsu15I ATCGAT 1 cut(s) 1024
BsuRI GGCC 3 cut(s) 49, 913, 1121
BsuTUI ATCGAT 1 cut(s) 1024
BtsCI GGATG 4 cut(s) 127, 164, 168, 184
BtsI GCAGTG 1 cut(s) 301
BtsIMutI CAGTG 4 cut(s) 301, 507, 747, 995
Cac8I GCNNGC 2 cut(s) 72, 1123
CfoI GCGC 1 cut(s) 167
Cfr13I GGNCC 2 cut(s) 787, 912
Cfr9I CCCGGG 1 cut(s) 916
ClaI ATCGAT 1 cut(s) 1024
Csp6I GTAC 2 cut(s) 452, 638
CviAII CATG 5 cut(s) 155, 704, 751, 768, 1210
CviQI GTAC 2 cut(s) 452, 638
DdeI CTNAG 4 cut(s) 777, 791, 998, 1134
DpnI GATC 6 cut(s) 272, 728, 828, 861, 885, 1023
DpnII GATC 6 cut(s) 270, 726, 826, 859, 883, 1021
DraI TTTAAA 1 cut(s) 376
Eam1104I CTCTTC 3 cut(s) 609, 680, 1101
EarI CTCTTC 3 cut(s) 609, 680, 1101
Eco105I TACGTA 1 cut(s) 981
Eco130I CCWWGG 3 cut(s) 225, 317, 326
Eco24I GRGCYC 2 cut(s) 219, 326
Eco47I GGWCC 1 cut(s) 787
Eco57I CTGAAG 2 cut(s) 456, 915
Eco88I CYCGRG 1 cut(s) 916
EcoT14I CCWWGG 3 cut(s) 225, 317, 326
EcoT38I GRGCYC 2 cut(s) 219, 326
ErhI CCWWGG 3 cut(s) 225, 317, 326
FaeI CATG 5 cut(s) 158, 707, 754, 771, 1213
FalI AAGNNNNNCTT 6 cut(s) 77, 109, 310, 342, 348, 380
FaqI GGGAC 2 cut(s) 575, 1078
FatI CATG 5 cut(s) 154, 703, 750, 767, 1209
FbaI TGATCA 1 cut(s) 270
Fnu4HI GCNGC 2 cut(s) 26, 68
FokI GGATG 4 cut(s) 134, 155, 171, 191
FriOI GRGCYC 2 cut(s) 219, 326
Fsp4HI GCNGC 2 cut(s) 26, 68
FspBI CTAG 4 cut(s) 263, 279, 284, 881
GlaI GCGC 1 cut(s) 166
GluI GCNGC 2 cut(s) 26, 68
GsuI CTGGAG 2 cut(s) 45, 62
HaeIII GGCC 3 cut(s) 49, 913, 1121
HapII CCGG 1 cut(s) 917
HhaI GCGC 1 cut(s) 167
Hin1II CATG 5 cut(s) 158, 707, 754, 771, 1213
Hin6I GCGC 1 cut(s) 165
HinP1I GCGC 1 cut(s) 165
HincII GTYRAC 1 cut(s) 478
HindII GTYRAC 1 cut(s) 478
HindIII AAGCTT 1 cut(s) 1053
HinfI GANTC 9 cut(s) 230, 332, 485, 650, 658, 665, 781, 877, 926
HpaI GTTAAC 1 cut(s) 478
HpaII CCGG 1 cut(s) 917
HphI GGTGA 1 cut(s) 313
Hpy166II GTNNAC 7 cut(s) 478, 507, 580, 669, 807, 960, 1187
Hpy188I TCNGA 4 cut(s) 649, 831, 864, 1135
Hpy8I GTNNAC 7 cut(s) 478, 507, 580, 669, 807, 960, 1187
Hpy99I CGWCG 2 cut(s) 528, 1091
HpyAV CCTTC 5 cut(s) 28, 480, 702, 889, 916
HpyCH4III ACNGT 2 cut(s) 642, 949
HpyCH4IV ACGT 4 cut(s) 9, 966, 980, 1086
HpyCH4V TGCA 8 cut(s) 140, 404, 681, 771, 807, 847, 1156, 1209
HpyF3I CTNAG 4 cut(s) 777, 791, 998, 1134
HpySE526I ACGT 4 cut(s) 9, 966, 980, 1086
Hsp92II CATG 5 cut(s) 158, 707, 754, 771, 1213
HspAI GCGC 1 cut(s) 165
Ksp22I TGATCA 1 cut(s) 270
KspAI GTTAAC 1 cut(s) 478
Kzo9I GATC 6 cut(s) 270, 726, 826, 859, 883, 1021
LmnI GCTCC 2 cut(s) 79, 321
Lsp1109I GCAGC 1 cut(s) 54
LweI GCATC 2 cut(s) 834, 861
MaeI CTAG 4 cut(s) 263, 279, 284, 881
MaeII ACGT 4 cut(s) 9, 966, 980, 1086
MaeIII GTNAC 5 cut(s) 55, 220, 746, 820, 1098
MalI GATC 6 cut(s) 272, 728, 828, 861, 885, 1023
MboI GATC 6 cut(s) 270, 726, 826, 859, 883, 1021
MboII GAAGA 8 cut(s) 192, 368, 626, 635, 667, 921, 1118, 1160
MflI RGATCY 2 cut(s) 859, 883
MhlI GDGCHC 3 cut(s) 219, 326, 809
MlyI GAGTC 4 cut(s) 239, 667, 674, 935
MmeI TCCRAC 1 cut(s) 449
MnlI CCTC 8 cut(s) 39, 87, 292, 378, 522, 610, 613, 900
MroXI GAANNNNTTC 2 cut(s) 191, 1191
MseI TTAA 8 cut(s) 95, 111, 207, 375, 477, 867, 975, 1043
MslI CAYNNNNRTG 1 cut(s) 157
MspI CCGG 1 cut(s) 917
MspR9I CCNGG 2 cut(s) 917, 918
MvnI CGCG 1 cut(s) 383
NciI CCSGG 2 cut(s) 917, 918
NdeII GATC 6 cut(s) 270, 726, 826, 859, 883, 1021
NlaIII CATG 5 cut(s) 158, 707, 754, 771, 1213
NlaIV GGNNCC 3 cut(s) 323, 467, 914
NmuCI GTSAC 1 cut(s) 55
NspI RCATGY 1 cut(s) 1213
NspV TTCGAA 1 cut(s) 612
PdmI GAANNNNTTC 2 cut(s) 191, 1191
PfeI GAWTC 5 cut(s) 332, 485, 650, 781, 877
PkrI GCNGC 2 cut(s) 27, 69
PleI GAGTC 4 cut(s) 238, 666, 673, 934
PpsI GAGTC 4 cut(s) 238, 666, 673, 934
Ppu21I YACGTR 2 cut(s) 967, 981
PshBI ATTAAT 1 cut(s) 867
PspN4I GGNNCC 3 cut(s) 323, 467, 914
PspPI GGNCC 2 cut(s) 787, 912
PstI CTGCAG 1 cut(s) 683
PsuI RGATCY 2 cut(s) 859, 883
RsaI GTAC 2 cut(s) 453, 639
RsaNI GTAC 2 cut(s) 452, 638
RseI CAYNNNNRTG 1 cut(s) 157
SaqAI TTAA 8 cut(s) 95, 111, 207, 375, 477, 867, 975, 1043
SatI GCNGC 2 cut(s) 26, 68
Sau3AI GATC 6 cut(s) 270, 726, 826, 859, 883, 1021
Sau96I GGNCC 2 cut(s) 787, 912
ScaI AGTACT 1 cut(s) 453
SchI GAGTC 4 cut(s) 239, 667, 674, 935
ScrFI CCNGG 2 cut(s) 917, 918
SduI GDGCHC 3 cut(s) 219, 326, 809
SfaNI GCATC 2 cut(s) 834, 861
SfcI CTRYAG 1 cut(s) 679
SfuI TTCGAA 1 cut(s) 612
SinI GGWCC 1 cut(s) 787
SmaI CCCGGG 1 cut(s) 918
SmiMI CAYNNNNRTG 1 cut(s) 157
SmlI CTYRAG 3 cut(s) 212, 1142, 1177
SmoI CTYRAG 3 cut(s) 212, 1142, 1177
SnaBI TACGTA 1 cut(s) 981
SpeI ACTAGT 1 cut(s) 278
SsiI CCGC 1 cut(s) 25
SspMI CTAG 4 cut(s) 263, 279, 284, 881
StyD4I CCNGG 2 cut(s) 915, 916
StyI CCWWGG 3 cut(s) 225, 317, 326
TaaI ACNGT 2 cut(s) 642, 949
TaiI ACGT 4 cut(s) 12, 969, 983, 1089
TaqI TCGA 3 cut(s) 32, 612, 1024
TatI WGTACW 2 cut(s) 451, 637
TauI GCSGC 1 cut(s) 28
TfiI GAWTC 5 cut(s) 332, 485, 650, 781, 877
Tru1I TTAA 8 cut(s) 95, 111, 207, 375, 477, 867, 975, 1043
Tru9I TTAA 8 cut(s) 95, 111, 207, 375, 477, 867, 975, 1043
TscAI CASTG 4 cut(s) 301, 514, 747, 1002
TseFI GTSAC 1 cut(s) 55
TseI GCWGC 1 cut(s) 67
Tsp45I GTSAC 1 cut(s) 55
TspDTI ATGAA 6 cut(s) 138, 405, 410, 710, 756, 981
TspGWI ACGGA 2 cut(s) 541, 872
TspMI CCCGGG 1 cut(s) 916
TspRI CASTG 4 cut(s) 301, 514, 747, 1002
VneI GTGCAC 1 cut(s) 805
VpaK11BI GGWCC 1 cut(s) 787
VspI ATTAAT 1 cut(s) 867
XapI RAATTY 3 cut(s) 244, 499, 988
XbaI TCTAGA 2 cut(s) 283, 880
XceI RCATGY 1 cut(s) 1213
XcmI CCANNNNNNNNNTGG 2 cut(s) 152, 324
XmaI CCCGGG 1 cut(s) 916
XmnI GAANNNNTTC 2 cut(s) 191, 1191
XspI CTAG 4 cut(s) 263, 279, 284, 881
ZrmI AGTACT 1 cut(s) 453
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.