MD12G1254700.v1.1

transposition, RNA-mediated

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0001068
Physical Location & Seq
Forward (+)
32308186 .. 32311620
3435 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1254700.v1.1.491

Sequence Viewer

Length: 1875 bp
ATGGCGTCCAACTCTTCCAGCTCTTCCAACGTTTCCAACTTTCTTCCGATTAAACTTGACCCTACCAATTACCCTCTCTGGCAAGCCCAGATGCTTACCTTGCTTCGTAGCAGGAATCTCGTCTCCTTTGTTGATGGCACTAGCAAATGTCCATGGATTCAACAAGATAATATGGTTTTGTCCTGGATCAAGAGCTCTGTTCATCCCACTGTGTTGGATGCCGTAATCTGGAAAACCAGTTCTCATTCTGCGTGGACCTGTCTGCGCGAAAGCTATGATTTGCAGCCCACTGGCCGTCTTCTTCAACTTCGCAGTGAGCTGATGAACACCCACCGTGGCGACTCCTCCATTTCTGAGTTTCTCAGTCGTGTCAGTTCCCTTGCTGACGGCCTCTCCTTATCTGGTGCTCCTGTTTCTGACTCAGACATTGCTGCCATTGTTCTCAACAATGTTGGCCCTGCATGTGAAAGCACCGTGGCTTCTGCTCAGGATCGTGATGGGGCCATATCTCACAGTGCTTCAGAGGCCCTTCTGCTCACTGCTGAGCGGAAACAAAGGATGCATACTGTCTTCTCTGCTGATACTAGACCCACTGCTCCCACTGCTGCTCGTGGCCGTCGTCCTGGCACCTTACGCGGTCGTGGCAAAGGCTATAGAGGCATAAGTGCTAGTTCAGGAATGCCTCACTTTCCACCACACAAGTGGCACTCGAAGGAATCGGAGAGGCCATTGCCGACTTCAGAGCTACTTGCTCAACGATTCAAGAAAAACTTATATGTCAAGCCATATAACAAGTCTAATGTGGATTGGACTGGAAGAATAGCTTATGCAGACCATTCTACATATAGATGGTGCGCTGTTGCTTTGCCTGACGAGAACCAATTTCCACCTTCTGTCAATCTTAAGCCCATTTGTTTGGAGTCCACTGAGCCGAAGGTTGAAGAAAATCGTCTAGCTTTAATCAACACTAGTCTAGATGAAGTGTGGTTGGAATGGAATTGGCTAAGGAGCCCTGCGGTATCTATAGCAGAAAATGTGCTGGAAGACTTGATTTCTTCTTTTAAACACATGAGGAATGAAATGAAGTGCGGAAAGCCCACTTTGATTGCTAGAGTGGGGAAAGTACTTTTTCGTAGGAGCCCTTTAGTTAACATGGAAGACATCAGAACAAATTTGTGTACTGAAATCTTGAAACAGTGGAGACGATCATTGTACACAAACATTCCTGTTTCATATAAGGAAAGGATTGTGAACGAAGTTGTCCCGAAAATTGGAGTTGATTTCGAAGGGGAGAAAGATACATACCAAGTAAAGTTGTCTGATTCCACCAGACCAGATGCAACTCTCTCCTGCAAATGCCGTGTAATGAAAGAACATGGAAAGTTACAACCCTACAAGATTGAACTGAATCAACTGCGTAACATGGTCGTGGATATTTCATGCACTACCAAAAATCTGGACCTGAGACTGATGCTATGCACGAAGAGACTCGTCACAGATCTGACTGACGATGAGATGCAAAGCATTTGGGATCTGATTAATTCCGCGATTCTAGATCCAGATGTGAAGGGCGGGTTGAGATGGCCTTCGGGAAAGGATTCTTCCGGAGATAAATACAAAGTTGATGGGGTTTGGCACTTGATAGCTAATACGTATAAAAATTCATCGTTCAGACTGAAAGTCAGACATGCGGATCGATTTGATTTTAGAACCCTATCTGGGGAAGCTACTTGGGAGACAAGTCTGATGTTGAAAAAGGTCCTGTCAAAGTTGGAGGAAGAGAATGCCGAAGCCAGCTCGGTTTCTGAGATACTGAAGGAAGACATGCATCTGATATGGGACAACTTCTTGAGCTGTGAACGTTTCCTGACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

625

Amino Acids

70.52

Weight (kDa)

8.66

Isoelectric Point (pI)

45.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Retrotran_gag_2 PF14223 47 - 187 6.3e-11 gag-polypeptide of LTR copia-type
DUF7903 PF25475 269 - 616 2.3e-145 Domain of unknown function (DUF7903)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 626
AccBSI CCGCTC 1 cut(s) 547
AccII CGCG 3 cut(s) 267, 636, 1547
AccIII TCCGGA 1 cut(s) 1604
AciI CCGC 7 cut(s) 547, 636, 1016, 1089, 1545, 1572, 1691
AclI AACGTT 2 cut(s) 30, 1861
AclWI GGATC 5 cut(s) 194, 498, 1539, 1550, 1701
AcoI YGGCCR 2 cut(s) 292, 613
AcsI RAATTY 2 cut(s) 1171, 1660
AcuI CTGAAG 3 cut(s) 504, 723, 1835
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 3 cut(s) 1125, 1180, 1214
AfiI CCNNNNNNNGG 4 cut(s) 228, 1271, 1719, 1720
AflII CTTAAG 1 cut(s) 902
AgsI TTSAA 7 cut(s) 161, 305, 763, 941, 1192, 1403, 1753
AhdI GACNNNNNGTC 1 cut(s) 1679
AhlI ACTAGT 1 cut(s) 968
AjnI CCWGG 2 cut(s) 182, 622
AleI CACNNNNGTG 1 cut(s) 700
AloI GAACNNNNNNTCC 2 cut(s) 223, 255
Alw21I GWGCWC 2 cut(s) 197, 409
Alw26I GTCTC 5 cut(s) 127, 1195, 1459, 1480, 1730
AlwI GGATC 5 cut(s) 194, 498, 1539, 1550, 1701
Aor13HI TCCGGA 1 cut(s) 1604
AoxI GGCC 8 cut(s) 292, 388, 454, 501, 525, 613, 725, 1583
ApeKI GCWGC 3 cut(s) 283, 431, 605
ApoI RAATTY 2 cut(s) 1171, 1660
AseI ATTAAT 1 cut(s) 1539
Asp700I GAANNNNTTC 1 cut(s) 1597
AspLEI GCGC 2 cut(s) 267, 857
AspS9I GGNCC 6 cut(s) 255, 455, 501, 526, 1459, 1759
AsuII TTCGAA 1 cut(s) 1284
AvaII GGWCC 3 cut(s) 255, 1459, 1759
BanI GGYRCC 1 cut(s) 626
BanII GRGCYC 3 cut(s) 197, 1013, 1142
BauI CACGAG 1 cut(s) 609
BbsI GAAGAC 5 cut(s) 290, 562, 1050, 1164, 1827
Bbv12I GWGCWC 2 cut(s) 197, 409
BbvI GCAGC 3 cut(s) 295, 418, 592
BccI CCATC 5 cut(s) 128, 491, 843, 1575, 1619
BceAI ACGGC 5 cut(s) 206, 279, 403, 600, 1344
BcgI CGANNNNNNTGC 2 cut(s) 100, 134
BciT130I CCWGG 2 cut(s) 184, 624
BcoDI GTCTC 5 cut(s) 127, 1195, 1459, 1480, 1730
BcuI ACTAGT 1 cut(s) 968
BfaI CTAG 8 cut(s) 141, 585, 669, 953, 969, 974, 1110, 1553
BfmI CTRYAG 2 cut(s) 652, 1023
BfrI CTTAAG 1 cut(s) 902
BglII AGATCT 1 cut(s) 1498
BisI GCNGC 3 cut(s) 284, 432, 606
BlpI GCTNAGC 1 cut(s) 543
BlsI GCNGC 3 cut(s) 285, 433, 607
BmcAI AGTACT 1 cut(s) 1125
Bme1390I CCNGG 2 cut(s) 184, 624
Bme18I GGWCC 3 cut(s) 255, 1459, 1759
BmeRI GACNNNNNGTC 1 cut(s) 1679
BmgT120I GGNCC 6 cut(s) 255, 455, 501, 526, 1459, 1759
BmiI GGNNCC 4 cut(s) 502, 628, 1010, 1139
BmrFI CCNGG 2 cut(s) 184, 624
BmsI GCATC 7 cut(s) 81, 208, 549, 1327, 1461, 1506, 1837
BpiI GAAGAC 5 cut(s) 290, 562, 1050, 1164, 1827
Bpu10I CCTNAGC 2 cut(s) 486, 1004
Bpu1102I GCTNAGC 1 cut(s) 543
Bpu14I TTCGAA 1 cut(s) 1284
BpuEI CTTGAG 1 cut(s) 1870
Bsa29I ATCGAT 1 cut(s) 1696
BsaAI YACGTR 1 cut(s) 1653
BsaHI GRCGYC 1 cut(s) 5
BsaJI CCNNGG 3 cut(s) 152, 334, 474
BsaWI WCCGGW 1 cut(s) 1604
BsaXI ACNNNNNCTCC 2 cut(s) 377, 407
Bsc4I CCNNNNNNNGG 4 cut(s) 228, 1271, 1719, 1720
Bse1I ACTGG 3 cut(s) 237, 295, 817
Bse3DI GCAATG 2 cut(s) 426, 728
BseAI TCCGGA 1 cut(s) 1604
BseBI CCWGG 2 cut(s) 184, 624
BseCI ATCGAT 1 cut(s) 1696
BseDI CCNNGG 3 cut(s) 152, 334, 474
BseGI GGATG 3 cut(s) 202, 223, 564
BseLI CCNNNNNNNGG 4 cut(s) 228, 1271, 1719, 1720
BseMI GCAATG 2 cut(s) 426, 728
BseMII CTCAG 8 cut(s) 345, 376, 435, 500, 534, 918, 1454, 1797
BseNI ACTGG 3 cut(s) 237, 295, 817
BseRI GAGGAG 1 cut(s) 334
BseXI GCAGC 3 cut(s) 295, 418, 592
Bsh1236I CGCG 3 cut(s) 267, 636, 1547
Bsh1285I CGRYCG 1 cut(s) 640
BshFI GGCC 8 cut(s) 294, 390, 456, 503, 527, 615, 727, 1585
BshNI GGYRCC 1 cut(s) 626
BshVI ATCGAT 1 cut(s) 1696
BsiEI CGRYCG 1 cut(s) 640
BsiHKAI GWGCWC 2 cut(s) 197, 409
BsiSI CCGG 1 cut(s) 1605
BslFI GGGAC 2 cut(s) 1247, 1853
BslI CCNNNNNNNGG 4 cut(s) 228, 1271, 1719, 1720
BsmAI GTCTC 5 cut(s) 127, 1195, 1459, 1480, 1730
BsmBI CGTCTC 2 cut(s) 127, 1195
BsmFI GGGAC 2 cut(s) 1247, 1853
BsmI GAATGC 2 cut(s) 684, 1789
BsnI GGCC 8 cut(s) 294, 390, 456, 503, 527, 615, 727, 1585
Bsp119I TTCGAA 1 cut(s) 1284
Bsp1286I GDGCHC 4 cut(s) 197, 409, 1013, 1142
Bsp13I TCCGGA 1 cut(s) 1604
Bsp1407I TGTACA 1 cut(s) 1212
Bsp143I GATC 7 cut(s) 186, 490, 1205, 1498, 1531, 1555, 1693
Bsp1720I GCTNAGC 1 cut(s) 543
Bsp19I CCATGG 1 cut(s) 152
BspACI CCGC 7 cut(s) 547, 636, 1016, 1089, 1545, 1572, 1691
BspANI GGCC 8 cut(s) 294, 390, 456, 503, 527, 615, 727, 1585
BspCNI CTCAG 8 cut(s) 346, 375, 434, 499, 535, 919, 1455, 1798
BspDI ATCGAT 1 cut(s) 1696
BspEI TCCGGA 1 cut(s) 1604
BspFNI CGCG 3 cut(s) 267, 636, 1547
BspLI GGNNCC 4 cut(s) 502, 628, 1010, 1139
BspPI GGATC 5 cut(s) 194, 498, 1539, 1550, 1701
BspQI GCTCTTC 1 cut(s) 28
BspT104I TTCGAA 1 cut(s) 1284
BspT107I GGYRCC 1 cut(s) 626
BspTI CTTAAG 1 cut(s) 902
BsrBI CCGCTC 1 cut(s) 547
BsrDI GCAATG 2 cut(s) 426, 728
BsrGI TGTACA 1 cut(s) 1212
BsrI ACTGG 3 cut(s) 237, 295, 817
BssECI CCNNGG 3 cut(s) 152, 334, 474
BssMI GATC 7 cut(s) 186, 490, 1205, 1498, 1531, 1555, 1693
BssNI GRCGYC 1 cut(s) 5
BssSI CACGAG 1 cut(s) 609
BssT1I CCWWGG 1 cut(s) 152
Bst2BI CACGAG 1 cut(s) 609
Bst2UI CCWGG 2 cut(s) 184, 624
Bst4CI ACNGT 6 cut(s) 211, 335, 475, 515, 568, 1197
Bst6I CTCTTC 4 cut(s) 19, 28, 1478, 1773
BstACI GRCGYC 1 cut(s) 5
BstAFI CTTAAG 1 cut(s) 902
BstAUI TGTACA 1 cut(s) 1212
BstBAI YACGTR 1 cut(s) 1653
BstBI TTCGAA 1 cut(s) 1284
BstC8I GCNNGC 2 cut(s) 84, 1795
BstDEI CTNAG 9 cut(s) 354, 362, 421, 486, 543, 927, 1004, 1463, 1806
BstDSI CCRYGG 3 cut(s) 152, 334, 474
BstF5I GGATG 3 cut(s) 202, 223, 564
BstFNI CGCG 3 cut(s) 267, 636, 1547
BstHHI GCGC 2 cut(s) 267, 857
BstKTI GATC 7 cut(s) 189, 493, 1208, 1501, 1534, 1558, 1696
BstMAI GTCTC 5 cut(s) 127, 1195, 1459, 1480, 1730
BstMBI GATC 7 cut(s) 186, 490, 1205, 1498, 1531, 1555, 1693
BstMCI CGRYCG 1 cut(s) 640
BstMWI GCNNNNNNNGC 6 cut(s) 100, 524, 602, 633, 642, 657
BstNI CCWGG 2 cut(s) 184, 624
BstNSI RCATGY 3 cut(s) 465, 1691, 1828
BstSCI CCNGG 2 cut(s) 182, 622
BstSFI CTRYAG 2 cut(s) 652, 1023
BstSNI TACGTA 1 cut(s) 1653
BstUI CGCG 3 cut(s) 267, 636, 1547
BstV1I GCAGC 3 cut(s) 295, 418, 592
BstV2I GAAGAC 5 cut(s) 290, 562, 1050, 1164, 1827
BstX2I RGATCY 3 cut(s) 1498, 1531, 1555
BstXI CCANNNNNNTGG 4 cut(s) 214, 702, 916, 1456
BstYI RGATCY 3 cut(s) 1498, 1531, 1555
Bsu15I ATCGAT 1 cut(s) 1696
BsuRI GGCC 8 cut(s) 294, 390, 456, 503, 527, 615, 727, 1585
BsuTUI ATCGAT 1 cut(s) 1696
BtgI CCRYGG 3 cut(s) 152, 334, 474
BtsCI GGATG 3 cut(s) 202, 223, 564
BtsI GCAGTG 4 cut(s) 319, 537, 591, 600
BtsIMutI CAGTG 9 cut(s) 207, 288, 319, 520, 537, 591, 600, 924, 1202
Cac8I GCNNGC 2 cut(s) 84, 1795
CfoI GCGC 2 cut(s) 267, 857
Cfr13I GGNCC 6 cut(s) 255, 455, 501, 526, 1459, 1759
ClaI ATCGAT 1 cut(s) 1696
Csp6I GTAC 3 cut(s) 1124, 1179, 1213
CspCI CAANNNNNGTGG 2 cut(s) 1087, 1122
CviQI GTAC 3 cut(s) 1124, 1179, 1213
DdeI CTNAG 9 cut(s) 354, 362, 421, 486, 543, 927, 1004, 1463, 1806
DpnI GATC 7 cut(s) 188, 492, 1207, 1500, 1533, 1557, 1695
DpnII GATC 7 cut(s) 186, 490, 1205, 1498, 1531, 1555, 1693
DraI TTTAAA 1 cut(s) 1063
DriI GACNNNNNGTC 1 cut(s) 1679
EaeI YGGCCR 2 cut(s) 292, 613
Eam1104I CTCTTC 4 cut(s) 19, 28, 1478, 1773
Eam1105I GACNNNNNGTC 1 cut(s) 1679
EarI CTCTTC 4 cut(s) 19, 28, 1478, 1773
Ecl136II GAGCTC 1 cut(s) 195
Eco105I TACGTA 1 cut(s) 1653
Eco130I CCWWGG 1 cut(s) 152
Eco24I GRGCYC 3 cut(s) 197, 1013, 1142
Eco47I GGWCC 3 cut(s) 255, 1459, 1759
Eco53kI GAGCTC 1 cut(s) 195
Eco57I CTGAAG 3 cut(s) 504, 723, 1835
EcoICRI GAGCTC 1 cut(s) 195
EcoO109I RGGNCCY 2 cut(s) 526, 1759
EcoRII CCWGG 2 cut(s) 182, 622
EcoT14I CCWWGG 1 cut(s) 152
EcoT22I ATGCAT 2 cut(s) 564, 1830
EcoT38I GRGCYC 3 cut(s) 197, 1013, 1142
ErhI CCWWGG 1 cut(s) 152
Esp3I CGTCTC 2 cut(s) 127, 1195
FalI AAGNNNNNCTT 4 cut(s) 755, 787, 808, 840
FaqI GGGAC 2 cut(s) 1247, 1853
FauI CCCGC 1 cut(s) 1565
Fnu4HI GCNGC 3 cut(s) 284, 432, 606
FokI GGATG 3 cut(s) 189, 230, 571
FriOI GRGCYC 3 cut(s) 197, 1013, 1142
Fsp4HI GCNGC 3 cut(s) 284, 432, 606
FspBI CTAG 8 cut(s) 141, 585, 669, 953, 969, 974, 1110, 1553
GlaI GCGC 2 cut(s) 266, 856
GluI GCNGC 3 cut(s) 284, 432, 606
HaeIII GGCC 8 cut(s) 294, 390, 456, 503, 527, 615, 727, 1585
HapII CCGG 1 cut(s) 1605
HhaI GCGC 2 cut(s) 267, 857
Hin1I GRCGYC 1 cut(s) 5
Hin6I GCGC 2 cut(s) 265, 855
HinP1I GCGC 2 cut(s) 265, 855
HincII GTYRAC 1 cut(s) 1150
HindII GTYRAC 1 cut(s) 1150
HpaI GTTAAC 1 cut(s) 1150
HpaII CCGG 1 cut(s) 1605
Hpy166II GTNNAC 7 cut(s) 255, 924, 1150, 1179, 1215, 1252, 1859
Hpy8I GTNNAC 7 cut(s) 255, 924, 1150, 1179, 1215, 1252, 1859
Hpy99I CGWCG 1 cut(s) 621
HpyAV CCTTC 8 cut(s) 539, 706, 900, 928, 1280, 1561, 1596, 1810
HpyCH4III ACNGT 6 cut(s) 211, 335, 475, 515, 568, 1197
HpyCH4IV ACGT 3 cut(s) 30, 1652, 1861
HpyF10VI GCNNNNNNNGC 6 cut(s) 100, 524, 602, 633, 642, 657
HpyF3I CTNAG 9 cut(s) 354, 362, 421, 486, 543, 927, 1004, 1463, 1806
HpySE526I ACGT 3 cut(s) 30, 1652, 1861
Hsp92I GRCGYC 1 cut(s) 5
HspAI GCGC 2 cut(s) 265, 855
Kpn2I TCCGGA 1 cut(s) 1604
KspAI GTTAAC 1 cut(s) 1150
Kzo9I GATC 7 cut(s) 186, 490, 1205, 1498, 1531, 1555, 1693
LguI GCTCTTC 1 cut(s) 28
LmnI GCTCC 4 cut(s) 412, 601, 1008, 1137
Lsp1109I GCAGC 3 cut(s) 295, 418, 592
LweI GCATC 7 cut(s) 81, 208, 549, 1327, 1461, 1506, 1837
MaeI CTAG 8 cut(s) 141, 585, 669, 953, 969, 974, 1110, 1553
MaeII ACGT 3 cut(s) 30, 1652, 1861
MaeIII GTNAC 3 cut(s) 1383, 1418, 1492
MalI GATC 7 cut(s) 188, 492, 1207, 1500, 1533, 1557, 1695
MbiI CCGCTC 1 cut(s) 547
MboI GATC 7 cut(s) 186, 490, 1205, 1498, 1531, 1555, 1693
MflI RGATCY 3 cut(s) 1498, 1531, 1555
MhlI GDGCHC 4 cut(s) 197, 409, 1013, 1142
MluCI AATT 7 cut(s) 67, 881, 997, 1171, 1269, 1540, 1660
MlyI GAGTC 4 cut(s) 335, 413, 929, 1482
MmeI TCCRAC 6 cut(s) 33, 51, 60, 195, 969, 1752
MnlI CCTC 9 cut(s) 84, 355, 401, 517, 650, 693, 717, 1065, 1768
Mph1103I ATGCAT 2 cut(s) 564, 1830
MroI TCCGGA 1 cut(s) 1604
MroXI GAANNNNTTC 1 cut(s) 1597
MseI TTAA 6 cut(s) 51, 903, 959, 1062, 1149, 1539
MslI CAYNNNNRTG 3 cut(s) 700, 847, 1427
MspCI CTTAAG 1 cut(s) 902
MspI CCGG 1 cut(s) 1605
MspR9I CCNGG 2 cut(s) 184, 624
Mva1269I GAATGC 2 cut(s) 684, 1789
MvaI CCWGG 2 cut(s) 184, 624
MvnI CGCG 3 cut(s) 267, 636, 1547
MwoI GCNNNNNNNGC 6 cut(s) 100, 524, 602, 633, 642, 657
NcoI CCATGG 1 cut(s) 152
NdeII GATC 7 cut(s) 186, 490, 1205, 1498, 1531, 1555, 1693
NlaIV GGNNCC 4 cut(s) 502, 628, 1010, 1139
NmuCI GTSAC 1 cut(s) 1492
NsiI ATGCAT 2 cut(s) 564, 1830
NspI RCATGY 3 cut(s) 465, 1691, 1828
NspV TTCGAA 1 cut(s) 1284
OliI CACNNNNGTG 1 cut(s) 700
PciSI GCTCTTC 1 cut(s) 28
PcsI WCGNNNNNNNCGW 1 cut(s) 616
PctI GAATGC 2 cut(s) 684, 1789
PdmI GAANNNNTTC 1 cut(s) 1597
PfeI GAWTC 8 cut(s) 115, 157, 716, 759, 1322, 1408, 1549, 1598
PfoI TCCNGGA 1 cut(s) 182
PkrI GCNGC 3 cut(s) 285, 433, 607
PleI GAGTC 4 cut(s) 335, 413, 928, 1482
PpsI GAGTC 4 cut(s) 335, 413, 928, 1482
Ppu21I YACGTR 1 cut(s) 1653
PpuMI RGGWCCY 1 cut(s) 1759
PshBI ATTAAT 1 cut(s) 1539
Psp124BI GAGCTC 1 cut(s) 197
Psp1406I AACGTT 2 cut(s) 30, 1861
Psp5II RGGWCCY 1 cut(s) 1759
Psp6I CCWGG 2 cut(s) 182, 622
PspGI CCWGG 2 cut(s) 182, 622
PspN4I GGNNCC 4 cut(s) 502, 628, 1010, 1139
PspPI GGNCC 6 cut(s) 255, 455, 501, 526, 1459, 1759
PspPPI RGGWCCY 1 cut(s) 1759
PsuI RGATCY 3 cut(s) 1498, 1531, 1555
RsaI GTAC 3 cut(s) 1125, 1180, 1214
RsaNI GTAC 3 cut(s) 1124, 1179, 1213
RseI CAYNNNNRTG 3 cut(s) 700, 847, 1427
SacI GAGCTC 1 cut(s) 197
SapI GCTCTTC 1 cut(s) 28
SaqAI TTAA 6 cut(s) 51, 903, 959, 1062, 1149, 1539
SatI GCNGC 3 cut(s) 284, 432, 606
Sau3AI GATC 7 cut(s) 186, 490, 1205, 1498, 1531, 1555, 1693
Sau96I GGNCC 6 cut(s) 255, 455, 501, 526, 1459, 1759
ScaI AGTACT 1 cut(s) 1125
SchI GAGTC 4 cut(s) 335, 413, 929, 1482
ScrFI CCNGG 2 cut(s) 184, 624
SduI GDGCHC 4 cut(s) 197, 409, 1013, 1142
SfaNI GCATC 7 cut(s) 81, 208, 549, 1327, 1461, 1506, 1837
SfcI CTRYAG 2 cut(s) 652, 1023
SfuI TTCGAA 1 cut(s) 1284
SinI GGWCC 3 cut(s) 255, 1459, 1759
SmiMI CAYNNNNRTG 3 cut(s) 700, 847, 1427
SmlI CTYRAG 2 cut(s) 902, 1849
SmoI CTYRAG 2 cut(s) 902, 1849
SnaBI TACGTA 1 cut(s) 1653
SpeI ACTAGT 1 cut(s) 968
Sse9I AATT 7 cut(s) 67, 881, 997, 1171, 1269, 1540, 1660
SsiI CCGC 7 cut(s) 547, 636, 1016, 1089, 1545, 1572, 1691
SspMI CTAG 8 cut(s) 141, 585, 669, 953, 969, 974, 1110, 1553
SstI GAGCTC 1 cut(s) 197
StyD4I CCNGG 2 cut(s) 182, 622
StyI CCWWGG 1 cut(s) 152
TaaI ACNGT 6 cut(s) 211, 335, 475, 515, 568, 1197
TaiI ACGT 3 cut(s) 33, 1655, 1864
TaqI TCGA 3 cut(s) 710, 1284, 1696
TasI AATT 7 cut(s) 67, 881, 997, 1171, 1269, 1540, 1660
TatI WGTACW 3 cut(s) 1123, 1178, 1212
TfiI GAWTC 8 cut(s) 115, 157, 716, 759, 1322, 1408, 1549, 1598
Tru1I TTAA 6 cut(s) 51, 903, 959, 1062, 1149, 1539
Tru9I TTAA 6 cut(s) 51, 903, 959, 1062, 1149, 1539
TscAI CASTG 9 cut(s) 214, 295, 319, 520, 544, 598, 607, 931, 1202
TseFI GTSAC 1 cut(s) 1492
TseI GCWGC 3 cut(s) 283, 431, 605
Tsp45I GTSAC 1 cut(s) 1492
TspDTI ATGAA 9 cut(s) 191, 338, 993, 1092, 1097, 1221, 1382, 1428, 1653
TspRI CASTG 9 cut(s) 214, 295, 319, 520, 544, 598, 607, 931, 1202
Vha464I CTTAAG 1 cut(s) 902
VpaK11BI GGWCC 3 cut(s) 255, 1459, 1759
VspI ATTAAT 1 cut(s) 1539
XapI RAATTY 2 cut(s) 1171, 1660
XbaI TCTAGA 2 cut(s) 973, 1552
XceI RCATGY 3 cut(s) 465, 1691, 1828
XcmI CCANNNNNNNNNTGG 1 cut(s) 699
XmnI GAANNNNTTC 1 cut(s) 1597
XspI CTAG 8 cut(s) 141, 585, 669, 953, 969, 974, 1110, 1553
ZrmI AGTACT 1 cut(s) 1125
Zsp2I ATGCAT 2 cut(s) 564, 1830
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.