pycom12g23270

Encoded by

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0001068
Physical Location & Seq
Forward (+)
23918110 .. 23922051
3942 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g23270.7

Sequence Viewer

Length: 1365 bp
ATGTCTACGAAACCTCACAAGGCAATGAACAGAGTAAAGCTACCACAATTTCTTATCTTAAATATTTTTTTGGTCTCCCAAGCTTTCAACTTTCTATCTGCCTCTCTTGGTAGTGATTTCTGGGTAAGCATAAGTGCTAGTTCTACAATGGCTTACGTTCCACCACACAGGCGGCACTCGAAGGGACCGGAGAGGCCATTGCTCACTCCAGAGCTGCTTGCTCCTCAATCCAAGAAAAACTTAAATGTCAAGCCATATAATAAGCCTATTGTGGATTGGACTGAAAATATAGTTTATGCAGACCATTCTACACATGGATGGTGCGCCATCCCTTTGGATGATGAGATCCAGTTTCCATCTTCTGTTAATCTTGAGCCCGTTACCTTGGAGTCTGCTGAGCTGAATTTTGGAGGCAATCATCTAGCTTTATCGATCAATACCAGTCTAGTTAATGGCGGTGAGGTGAAGTGGAACTTGCCAAGGAGCCCTTGGGAATCTATAACAGAAAATGTGCTGGAAGACCTACTTTCGGCATTTAAACACGTGAGGAATGAAATGAAGTCTGCAAAGCCCAAAGAAGCATGTCCAACTCTGATTGCCAGAGTGGGGAAAGTACTCTTTCGCAGGAACCGTTCAGTTAACATGGAATCCATCAGAAAAAATTTGCGTACTGAAATCTTGAAACAATGGAGGCGATTATTTTACATAAACACTCCTGTTTCATATAAGGAAAAGATTGTGAACAAAGTTGTCCCGAAAATCGGAGTAGATTTCGAAGAGGAGGAAGATACATACGAATTACAGTTGTCTGATTCTACGAGTCCAGAGTCCACTCTCTTCTGCAGATGTCGGGTAATGAAAGAACATGGAAAGCTACAACTCTGTGAGATCAAGTTGAAGCCAGTGCGTAACATGGTAATGGACATTTCATGCACTACTAAGAATCTGGACCTGAGACTAATGCTGTGCACGGAGAAACATGTAACTGATCTAACTAATGATGAGATGCAAAGCATCACGGATCTGATTAATTCTGCGATTCTAGATCCAGATTTGAAGGGTGGACTGAGGTGGCCCCCGGGGAAGGAGTCTTCTGGAGATAAATACACAGTTGTTAGGTTTTGTCACGGAATAGGTAGTACGTATAGAAATTCATCACTGAGACTGAAAGTAAAACACTATGATCGATTTGATTTGAGAACCTTAACTGGGGAAGCTTCTTGGGGGATAAGTCTGATGCTGGAAAACGTCATGTCAAAGTTACAGGAAGAGAATTTTGAAGCCAGCACAATTTCTGAGATACTCAAGGAAGATGTGCAGTTGATATGGGATAACTTCTTGAGCTGTGAACGTTTTCTAACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

455

Amino Acids

51.87

Weight (kDa)

7.99

Isoelectric Point (pI)

49.85

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 5
AciI CCGC 2 cut(s) 172, 456
AclI AACGTT 1 cut(s) 1351
AclWI GGATC 3 cut(s) 340, 1029, 1040
AcsI RAATTY 4 cut(s) 403, 661, 1150, 1273
AcvI CACGTG 1 cut(s) 544
AfaI GTAC 3 cut(s) 615, 670, 1141
AfiI CCNNNNNNNGG 5 cut(s) 529, 606, 761, 1084, 1209
AflIII ACRYGT 2 cut(s) 541, 979
AgsI TTSAA 5 cut(s) 88, 682, 898, 1057, 1280
AluBI AGCT 8 cut(s) 40, 83, 214, 400, 425, 874, 1217, 1344
AluI AGCT 8 cut(s) 40, 83, 214, 400, 425, 874, 1217, 1344
Alw21I GWGCWC 1 cut(s) 971
Alw26I GTCTC 3 cut(s) 79, 949, 1156
Alw44I GTGCAC 1 cut(s) 967
AlwI GGATC 3 cut(s) 340, 1029, 1040
Ama87I CYCGRG 1 cut(s) 1078
AoxI GGCC 2 cut(s) 194, 1073
ApaLI GTGCAC 1 cut(s) 967
ApeKI GCWGC 1 cut(s) 214
ApoI RAATTY 4 cut(s) 403, 661, 1150, 1273
AseI ATTAAT 1 cut(s) 1029
Asp700I GAANNNNTTC 1 cut(s) 1353
AspLEI GCGC 1 cut(s) 326
AspS9I GGNCC 3 cut(s) 185, 949, 1074
AsuC2I CCSGG 2 cut(s) 1079, 1080
AsuHPI GGTGA 2 cut(s) 470, 475
AsuII TTCGAA 1 cut(s) 774
AvaI CYCGRG 1 cut(s) 1078
AvaII GGWCC 2 cut(s) 185, 949
BaeGI GKGCMC 1 cut(s) 971
BanII GRGCYC 2 cut(s) 378, 488
BbrPI CACGTG 1 cut(s) 544
BbsI GAAGAC 2 cut(s) 525, 1083
Bbv12I GWGCWC 1 cut(s) 971
BbvI GCAGC 1 cut(s) 201
BccI CCATC 4 cut(s) 312, 335, 364, 659
BcnI CCSGG 2 cut(s) 1079, 1080
BcoDI GTCTC 3 cut(s) 79, 949, 1156
BfaI CTAG 4 cut(s) 138, 422, 446, 1043
BfmI CTRYAG 1 cut(s) 841
BisI GCNGC 2 cut(s) 173, 215
BlpI GCTNAGC 1 cut(s) 396
BlsI GCNGC 2 cut(s) 174, 216
BmcAI AGTACT 1 cut(s) 615
Bme1390I CCNGG 2 cut(s) 1079, 1080
Bme18I GGWCC 2 cut(s) 185, 949
BmeT110I CYCGRG 1 cut(s) 1078
BmgT120I GGNCC 3 cut(s) 185, 949, 1074
BmiI GGNNCC 4 cut(s) 186, 485, 629, 1076
BmrFI CCNGG 2 cut(s) 1079, 1080
BmrI ACTGGG 1 cut(s) 1218
BmsI GCATC 3 cut(s) 996, 1023, 1227
BmuI ACTGGG 1 cut(s) 1218
BpiI GAAGAC 2 cut(s) 525, 1083
BpmI CTGGAG 2 cut(s) 192, 1116
Bpu1102I GCTNAGC 1 cut(s) 396
Bpu14I TTCGAA 1 cut(s) 774
BpuEI CTTGAG 3 cut(s) 392, 1289, 1360
BpuMI CCSGG 2 cut(s) 1079, 1080
Bsa29I ATCGAT 2 cut(s) 431, 1186
BsaAI YACGTR 2 cut(s) 544, 1143
BsaI GGTCTC 1 cut(s) 79
BsaJI CCNNGG 6 cut(s) 384, 479, 488, 1077, 1078, 1079
BsaWI WCCGGW 1 cut(s) 187
Bsc4I CCNNNNNNNGG 5 cut(s) 529, 606, 761, 1084, 1209
Bse1I ACTGG 4 cut(s) 349, 441, 902, 1213
Bse3DI GCAATG 2 cut(s) 30, 197
BseCI ATCGAT 2 cut(s) 431, 1186
BseDI CCNNGG 6 cut(s) 384, 479, 488, 1077, 1078, 1079
BseGI GGATG 3 cut(s) 323, 327, 343
BseLI CCNNNNNNNGG 5 cut(s) 529, 606, 761, 1084, 1209
BseMI GCAATG 2 cut(s) 30, 197
BseMII CTCAG 5 cut(s) 387, 944, 1058, 1151, 1287
BseNI ACTGG 4 cut(s) 349, 441, 902, 1213
BseRI GAGGAG 2 cut(s) 213, 794
BseSI GKGCMC 1 cut(s) 971
BseXI GCAGC 1 cut(s) 201
BsgI GTGCAG 1 cut(s) 1337
BshFI GGCC 2 cut(s) 196, 1075
BshVI ATCGAT 2 cut(s) 431, 1186
BsiHKAI GWGCWC 1 cut(s) 971
BsiHKCI CYCGRG 1 cut(s) 1078
BsiSI CCGG 2 cut(s) 188, 1079
BslFI GGGAC 2 cut(s) 198, 737
BslI CCNNNNNNNGG 5 cut(s) 529, 606, 761, 1084, 1209
BsmAI GTCTC 3 cut(s) 79, 949, 1156
BsmFI GGGAC 2 cut(s) 198, 737
BsnI GGCC 2 cut(s) 196, 1075
Bso31I GGTCTC 1 cut(s) 79
BsoBI CYCGRG 1 cut(s) 1078
Bsp119I TTCGAA 1 cut(s) 774
Bsp1286I GDGCHC 3 cut(s) 378, 488, 971
Bsp143I GATC 7 cut(s) 345, 432, 888, 988, 1021, 1045, 1183
Bsp1720I GCTNAGC 1 cut(s) 396
BspACI CCGC 2 cut(s) 172, 456
BspANI GGCC 2 cut(s) 196, 1075
BspCNI CTCAG 5 cut(s) 388, 945, 1059, 1152, 1288
BspDI ATCGAT 2 cut(s) 431, 1186
BspLI GGNNCC 4 cut(s) 186, 485, 629, 1076
BspMAI CTGCAG 1 cut(s) 845
BspPI GGATC 3 cut(s) 340, 1029, 1040
BspT104I TTCGAA 1 cut(s) 774
BspTNI GGTCTC 1 cut(s) 79
BsrDI GCAATG 2 cut(s) 30, 197
BsrI ACTGG 4 cut(s) 349, 441, 902, 1213
BssECI CCNNGG 6 cut(s) 384, 479, 488, 1077, 1078, 1079
BssMI GATC 7 cut(s) 345, 432, 888, 988, 1021, 1045, 1183
BssT1I CCWWGG 3 cut(s) 384, 479, 488
Bst4CI ACNGT 3 cut(s) 632, 804, 1111
Bst6I CTCTTC 3 cut(s) 771, 842, 1263
BstBAI YACGTR 2 cut(s) 544, 1143
BstBI TTCGAA 1 cut(s) 774
BstC8I GCNNGC 2 cut(s) 219, 1285
BstDEI CTNAG 6 cut(s) 396, 939, 953, 1067, 1160, 1296
BstF5I GGATG 3 cut(s) 323, 327, 343
BstHHI GCGC 1 cut(s) 326
BstKTI GATC 7 cut(s) 348, 435, 891, 991, 1024, 1048, 1186
BstMAI GTCTC 3 cut(s) 79, 949, 1156
BstMBI GATC 7 cut(s) 345, 432, 888, 988, 1021, 1045, 1183
BstNSI RCATGY 2 cut(s) 585, 983
BstSCI CCNGG 2 cut(s) 1077, 1078
BstSFI CTRYAG 1 cut(s) 841
BstSLI GKGCMC 1 cut(s) 971
BstSNI TACGTA 1 cut(s) 1143
BstV1I GCAGC 1 cut(s) 201
BstV2I GAAGAC 2 cut(s) 525, 1083
BstX2I RGATCY 3 cut(s) 345, 1021, 1045
BstXI CCANNNNNNTGG 1 cut(s) 334
BstYI RGATCY 3 cut(s) 345, 1021, 1045
Bsu15I ATCGAT 2 cut(s) 431, 1186
BsuRI GGCC 2 cut(s) 196, 1075
BsuTUI ATCGAT 2 cut(s) 431, 1186
BtsCI GGATG 3 cut(s) 323, 327, 343
BtsIMutI CAGTG 2 cut(s) 909, 1157
Cac8I GCNNGC 2 cut(s) 219, 1285
CfoI GCGC 1 cut(s) 326
Cfr13I GGNCC 3 cut(s) 185, 949, 1074
Cfr9I CCCGGG 1 cut(s) 1078
ClaI ATCGAT 2 cut(s) 431, 1186
Csp6I GTAC 3 cut(s) 614, 669, 1140
CviAII CATG 9 cut(s) 314, 582, 643, 866, 913, 930, 980, 1252, 1362
CviQI GTAC 3 cut(s) 614, 669, 1140
DdeI CTNAG 6 cut(s) 396, 939, 953, 1067, 1160, 1296
DpnI GATC 7 cut(s) 347, 434, 890, 990, 1023, 1047, 1185
DpnII GATC 7 cut(s) 345, 432, 888, 988, 1021, 1045, 1183
DraI TTTAAA 1 cut(s) 538
Eam1104I CTCTTC 3 cut(s) 771, 842, 1263
EarI CTCTTC 3 cut(s) 771, 842, 1263
Eco105I TACGTA 1 cut(s) 1143
Eco130I CCWWGG 3 cut(s) 384, 479, 488
Eco24I GRGCYC 2 cut(s) 378, 488
Eco31I GGTCTC 1 cut(s) 79
Eco47I GGWCC 2 cut(s) 185, 949
Eco72I CACGTG 1 cut(s) 544
Eco88I CYCGRG 1 cut(s) 1078
EcoT14I CCWWGG 3 cut(s) 384, 479, 488
EcoT38I GRGCYC 2 cut(s) 378, 488
ErhI CCWWGG 3 cut(s) 384, 479, 488
FaeI CATG 9 cut(s) 317, 585, 646, 869, 916, 933, 983, 1255, 1365
FalI AAGNNNNNCTT 8 cut(s) 224, 256, 458, 490, 472, 504, 510, 542
FaqI GGGAC 2 cut(s) 198, 737
FatI CATG 9 cut(s) 313, 581, 642, 865, 912, 929, 979, 1251, 1361
FblI GTMKAC 1 cut(s) 5
Fnu4HI GCNGC 2 cut(s) 173, 215
FokI GGATG 3 cut(s) 314, 330, 350
FriOI GRGCYC 2 cut(s) 378, 488
Fsp4HI GCNGC 2 cut(s) 173, 215
FspBI CTAG 4 cut(s) 138, 422, 446, 1043
GlaI GCGC 1 cut(s) 325
GluI GCNGC 2 cut(s) 173, 215
GsuI CTGGAG 2 cut(s) 192, 1116
HaeIII GGCC 2 cut(s) 196, 1075
HapII CCGG 2 cut(s) 188, 1079
HhaI GCGC 1 cut(s) 326
Hin1II CATG 9 cut(s) 317, 585, 646, 869, 916, 933, 983, 1255, 1365
Hin6I GCGC 1 cut(s) 324
HinP1I GCGC 1 cut(s) 324
HincII GTYRAC 1 cut(s) 640
HindII GTYRAC 1 cut(s) 640
HindIII AAGCTT 2 cut(s) 81, 1215
HinfI GANTC 9 cut(s) 389, 494, 647, 812, 820, 827, 943, 1039, 1088
HpaI GTTAAC 1 cut(s) 640
HpaII CCGG 2 cut(s) 188, 1079
HphI GGTGA 2 cut(s) 470, 475
Hpy166II GTNNAC 7 cut(s) 6, 640, 742, 831, 969, 1064, 1349
Hpy188I TCNGA 7 cut(s) 594, 656, 764, 811, 1026, 1236, 1297
Hpy8I GTNNAC 7 cut(s) 6, 640, 742, 831, 969, 1064, 1349
HpyAV CCTTC 3 cut(s) 175, 1051, 1078
HpyCH4III ACNGT 3 cut(s) 632, 804, 1111
HpyCH4IV ACGT 5 cut(s) 156, 543, 1142, 1248, 1351
HpyCH4V TGCA 7 cut(s) 299, 566, 843, 933, 969, 1009, 1318
HpyF3I CTNAG 6 cut(s) 396, 939, 953, 1067, 1160, 1296
HpySE526I ACGT 5 cut(s) 156, 543, 1142, 1248, 1351
Hsp92II CATG 9 cut(s) 317, 585, 646, 869, 916, 933, 983, 1255, 1365
HspAI GCGC 1 cut(s) 324
KspAI GTTAAC 1 cut(s) 640
Kzo9I GATC 7 cut(s) 345, 432, 888, 988, 1021, 1045, 1183
LmnI GCTCC 2 cut(s) 226, 483
Lsp1109I GCAGC 1 cut(s) 201
LweI GCATC 3 cut(s) 996, 1023, 1227
MaeI CTAG 4 cut(s) 138, 422, 446, 1043
MaeII ACGT 5 cut(s) 156, 543, 1142, 1248, 1351
MaeIII GTNAC 5 cut(s) 379, 908, 982, 1124, 1260
MalI GATC 7 cut(s) 347, 434, 890, 990, 1023, 1047, 1185
MboI GATC 7 cut(s) 345, 432, 888, 988, 1021, 1045, 1183
MboII GAAGA 8 cut(s) 351, 530, 788, 797, 829, 1083, 1280, 1322
MflI RGATCY 3 cut(s) 345, 1021, 1045
MhlI GDGCHC 3 cut(s) 378, 488, 971
MluCI AATT 8 cut(s) 47, 403, 661, 797, 1030, 1150, 1273, 1290
MlyI GAGTC 4 cut(s) 398, 829, 836, 1097
MmeI TCCRAC 1 cut(s) 611
MroXI GAANNNNTTC 1 cut(s) 1353
MseI TTAA 8 cut(s) 59, 242, 366, 450, 537, 639, 1029, 1205
MslI CAYNNNNRTG 2 cut(s) 316, 917
MspI CCGG 2 cut(s) 188, 1079
MspR9I CCNGG 2 cut(s) 1079, 1080
NciI CCSGG 2 cut(s) 1079, 1080
NdeII GATC 7 cut(s) 345, 432, 888, 988, 1021, 1045, 1183
NlaIII CATG 9 cut(s) 317, 585, 646, 869, 916, 933, 983, 1255, 1365
NlaIV GGNNCC 4 cut(s) 186, 485, 629, 1076
NmuCI GTSAC 1 cut(s) 1124
NspI RCATGY 2 cut(s) 585, 983
NspV TTCGAA 1 cut(s) 774
PciI ACATGT 1 cut(s) 979
PcsI WCGNNNNNNNCGW 1 cut(s) 628
PdmI GAANNNNTTC 1 cut(s) 1353
PfeI GAWTC 5 cut(s) 494, 647, 812, 943, 1039
PkrI GCNGC 2 cut(s) 174, 216
PleI GAGTC 4 cut(s) 397, 828, 835, 1096
PmaCI CACGTG 1 cut(s) 544
PmlI CACGTG 1 cut(s) 544
PpsI GAGTC 4 cut(s) 397, 828, 835, 1096
Ppu21I YACGTR 2 cut(s) 544, 1143
PscI ACATGT 1 cut(s) 979
PshBI ATTAAT 1 cut(s) 1029
Psp1406I AACGTT 1 cut(s) 1351
PspCI CACGTG 1 cut(s) 544
PspN4I GGNNCC 4 cut(s) 186, 485, 629, 1076
PspPI GGNCC 3 cut(s) 185, 949, 1074
PstI CTGCAG 1 cut(s) 845
PsuI RGATCY 3 cut(s) 345, 1021, 1045
RsaI GTAC 3 cut(s) 615, 670, 1141
RsaNI GTAC 3 cut(s) 614, 669, 1140
RseI CAYNNNNRTG 2 cut(s) 316, 917
SaqAI TTAA 8 cut(s) 59, 242, 366, 450, 537, 639, 1029, 1205
SatI GCNGC 2 cut(s) 173, 215
Sau3AI GATC 7 cut(s) 345, 432, 888, 988, 1021, 1045, 1183
Sau96I GGNCC 3 cut(s) 185, 949, 1074
ScaI AGTACT 1 cut(s) 615
SchI GAGTC 4 cut(s) 398, 829, 836, 1097
ScrFI CCNGG 2 cut(s) 1079, 1080
SduI GDGCHC 3 cut(s) 378, 488, 971
SfaNI GCATC 3 cut(s) 996, 1023, 1227
SfcI CTRYAG 1 cut(s) 841
SfuI TTCGAA 1 cut(s) 774
SinI GGWCC 2 cut(s) 185, 949
SmaI CCCGGG 1 cut(s) 1080
SmiMI CAYNNNNRTG 2 cut(s) 316, 917
SmlI CTYRAG 3 cut(s) 371, 1304, 1339
SmoI CTYRAG 3 cut(s) 371, 1304, 1339
SnaBI TACGTA 1 cut(s) 1143
Sse9I AATT 8 cut(s) 47, 403, 661, 797, 1030, 1150, 1273, 1290
SsiI CCGC 2 cut(s) 172, 456
SspI AATATT 1 cut(s) 64
SspMI CTAG 4 cut(s) 138, 422, 446, 1043
StyD4I CCNGG 2 cut(s) 1077, 1078
StyI CCWWGG 3 cut(s) 384, 479, 488
TaaI ACNGT 3 cut(s) 632, 804, 1111
TaiI ACGT 5 cut(s) 159, 546, 1145, 1251, 1354
TaqI TCGA 4 cut(s) 179, 431, 774, 1186
TasI AATT 8 cut(s) 47, 403, 661, 797, 1030, 1150, 1273, 1290
TatI WGTACW 1 cut(s) 613
TauI GCSGC 1 cut(s) 175
TfiI GAWTC 5 cut(s) 494, 647, 812, 943, 1039
Tru1I TTAA 8 cut(s) 59, 242, 366, 450, 537, 639, 1029, 1205
Tru9I TTAA 8 cut(s) 59, 242, 366, 450, 537, 639, 1029, 1205
TscAI CASTG 2 cut(s) 909, 1164
TseFI GTSAC 1 cut(s) 1124
TseI GCWGC 1 cut(s) 214
Tsp45I GTSAC 1 cut(s) 1124
TspDTI ATGAA 7 cut(s) 41, 567, 572, 711, 872, 918, 1143
TspGWI ACGGA 3 cut(s) 986, 1034, 1143
TspMI CCCGGG 1 cut(s) 1078
TspRI CASTG 2 cut(s) 909, 1164
VneI GTGCAC 1 cut(s) 967
VpaK11BI GGWCC 2 cut(s) 185, 949
VspI ATTAAT 1 cut(s) 1029
XapI RAATTY 4 cut(s) 403, 661, 1150, 1273
XbaI TCTAGA 1 cut(s) 1042
XceI RCATGY 2 cut(s) 585, 983
XcmI CCANNNNNNNNNTGG 2 cut(s) 311, 486
XmaI CCCGGG 1 cut(s) 1078
XmiI GTMKAC 1 cut(s) 5
XmnI GAANNNNTTC 1 cut(s) 1353
XspI CTAG 4 cut(s) 138, 422, 446, 1043
ZrmI AGTACT 1 cut(s) 615
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.