MD12G1254500.v1.1

transposition, RNA-mediated

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0001068
Physical Location & Seq
Forward (+)
32290771 .. 32294329
3559 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1254500.v1.1.491

Sequence Viewer

Length: 1998 bp
ATGGCGTATAATTCTTCCAGCTCTTCCACCTCCATTCCTCTCCCTACCCTACCTGATGTTTCCACCTTCCTTAAGATTAAACTTGACCCTACCAATTACCCTCTCTGGCAAGTCCCGATGCTTACCTTGCTTCGTAGCAGGAATCTCGTCTCCTATGTTGATGGCACTAGCAAATGTCCTCCTGCCTTTCTCAAAGATGATGAGGGCATTTTTACTGATACTGTGAATCCGGAGTTTGAAGCATGGATTCAACAAGATGCTATGGTTATGTCCTGGATCAAGAGCTCTGTTCATCCCACTGTGTTGGGTGCCCTAATCGGGAAAACCAGTTCTCATTCTGCATGGATCTGTCTGCGCGAACGCTATGATTTGCAGTCCACTGGCCGTCTTCTTCAACTTCGCAGTGAGCTGATGAACACCCACCGTGGTGACTCCTCCATTGCTGAGTTTCTCGGTCGTGTCAATTGCCTCGCTGAGACTCTCTCCTTATCCGGTGCTCCTGTTTCTGAGTCAGATATTGTTGCCATTGTTCTCAACAATGTTGGTCCTGCATATGAAAGCACCGTGGCTTCTGCTCAGGCTCGTGATGAAGCCATATCTTACAGTGCTTTGGAGGCCCTTCTGCTCAGCGCTGAGCGCAGTCAAGAGATGGATACAGTCTTCTCTGATGATACTGGACCTACTGCTCTCGCTGCTGCTTGTGGCGGTTGTCCTGGTACCTTTCGTGGTTGTGGCGAAGGCTATGGACGTATTCGTGGTGGCACAAGTGCTAGTTCTAGAATGGCTTACATTCCAACACACAAGCGGCACTTGAAGGAATCGGAGAGGACATTGCTGACATCAGAGCTGCTTGCTTCACAATTGAAGAAAAACTTAAATGTCAATCCATGTAACATGTCTAATGTGGATGGGACTGGAAGAATAGTTTATGCAGACCATCCTACACATAGATGGTGTGCCGTAGCTTTGGATGACGAGAACCAGTTTCCATCTTCTGTTAATCTTGAACCTGTTACCTTGGAGTCTGCTGAGCCGAAAATTGGAAATAAATTTCTAGCTTTGATCAATACTAGTATAGATAACGAAGGCGGTGAGGTGAAATTGAATTCGCGAAGGAGCCTTGGGGCATCTATAGCAGAAAATGTGCTGGAAGACTTAGTTTCTTCTTTCGAACACATGAGGAATGAAATGAAGTGTGCAAAGCTCACAGAAGTAAATCCTACTTTGGTTGCTAGAGTGGGCAAAGTACTTTTTCGTAGGGTCCCTTCAGTTAGCATCGAATCCATCAGAAGAAATTTGTGTCCTGAAATCTTGAAACGATGGGGGCGATCGTTTTACCCAAATGTTCCTGTTTCATATAAGGAAAGGATTGTGAACGAAGTTGTCCCACAAATTGGAGTTGATTTCGAAGAGGAGGAAGATATATACGAATTACAGTTGTCTGATTCTACGAGTCCAGATCCAACTCTCTCCTGCAAATGTCGGGTAATGAAGGAACATGGAACGCTACAACTCTACGAGATCAAATTGAAACCAGTGCGTAAAATGGTAATGGACATTTCATGCACTACTAAGAATCTGGACCTGAGGCTAATGCTATGCACTAAGAGACTCGTAACTGATCTGACTGATGATGAGATGCAAAGCATTACGGATCTCGTTAATTCTGCGATTCTAGATCCAGAAGTGAAGGGTGGATTGAGATGGCCCCTGGGGAAGGAGTCTTCTGGAGATAAATACAAAGTTCTTAGGGTTTGGCACGTAAGAGCTAATACGTATAGAAATTCATCACTGAGATTGAAAGTAAAACACTATGACCGATTTGATTTTAGAACCCTAACCGGGGAAGCTTCTTGGGTGGCGAGTCTGGTGCTGGAAAACGTAATGTCAAAGTTACGGGAAGAAAATGTCGAAGTCAGCTCGGTTTATGAGATGCTTAAGGAAAATATGCAGTTTATATGGGATAACTTCTTGAGCTGTGAAAGTTTTTTAACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

666

Amino Acids

74.21

Weight (kDa)

5.67

Isoelectric Point (pI)

47.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Retrotran_gag_2 PF14223 41 - 218 6e-16 gag-polypeptide of LTR copia-type
DUF7903 PF25475 304 - 657 7.6e-153 Domain of unknown function (DUF7903)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 716
AccB1I GGYRCC 2 cut(s) 308, 716
AccB7I CCANNNNNTGG 1 cut(s) 1394
AccII CGCG 2 cut(s) 357, 1111
AccIII TCCGGA 1 cut(s) 229
AciI CCGC 3 cut(s) 705, 805, 1089
AclWI GGATC 5 cut(s) 284, 353, 1454, 1662, 1673
AcoI YGGCCR 1 cut(s) 382
AcsI RAATTY 4 cut(s) 1049, 1105, 1294, 1783
AcuI CTGAAG 1 cut(s) 1251
AfaI GTAC 2 cut(s) 718, 1248
AfeI AGCGCT 1 cut(s) 631
AfiI CCNNNNNNNGG 6 cut(s) 318, 1040, 1394, 1717, 1842, 1843
AflII CTTAAG 2 cut(s) 71, 1937
AflIII ACRYGT 1 cut(s) 894
AhlI ACTAGT 1 cut(s) 1070
AjnI CCWGG 3 cut(s) 272, 712, 1710
AleI CACNNNNGTG 1 cut(s) 426
AloI GAACNNNNNNTCC 2 cut(s) 313, 345
Alw21I GWGCWC 2 cut(s) 287, 499
Alw26I GTCTC 3 cut(s) 154, 470, 1603
AlwI GGATC 5 cut(s) 284, 353, 1454, 1662, 1673
Aor13HI TCCGGA 1 cut(s) 229
Aor51HI AGCGCT 1 cut(s) 631
AoxI GGCC 3 cut(s) 382, 615, 1706
ApeKI GCWGC 3 cut(s) 692, 695, 847
ApoI RAATTY 4 cut(s) 1049, 1105, 1294, 1783
Asp718I GGTACC 1 cut(s) 716
AspLEI GCGC 3 cut(s) 357, 632, 639
AspS9I GGNCC 6 cut(s) 545, 616, 677, 1261, 1582, 1707
AsuC2I CCSGG 1 cut(s) 1843
AsuHPI GGTGA 3 cut(s) 440, 1103, 1108
AsuII TTCGAA 2 cut(s) 1170, 1407
AvaII GGWCC 4 cut(s) 545, 677, 1261, 1582
AxyI CCTNAGG 1 cut(s) 1586
BaeGI GKGCMC 1 cut(s) 313
BanI GGYRCC 2 cut(s) 308, 716
BanII GRGCYC 1 cut(s) 287
BarI GAAGNNNNNNTAC 2 cut(s) 1249, 1281
BauI CACGAG 1 cut(s) 582
BbsI GAAGAC 4 cut(s) 380, 652, 1158, 1716
Bbv12I GWGCWC 2 cut(s) 287, 499
BbvI GCAGC 3 cut(s) 679, 682, 834
BccI CCATC 9 cut(s) 155, 643, 902, 945, 945, 997, 1292, 1314, 1698
BceAI ACGGC 2 cut(s) 369, 944
BcgI CGANNNNNNTGC 4 cut(s) 127, 161, 1852, 1886
BciT130I CCWGG 3 cut(s) 274, 714, 1712
BciVI GTATCC 1 cut(s) 646
BclI TGATCA 1 cut(s) 1062
BcnI CCSGG 1 cut(s) 1843
BcoDI GTCTC 3 cut(s) 154, 470, 1603
BcuI ACTAGT 1 cut(s) 1070
BfaI CTAG 7 cut(s) 168, 771, 777, 1055, 1071, 1233, 1676
BfmI CTRYAG 1 cut(s) 1131
BfoI RGCGCY 1 cut(s) 633
BfrI CTTAAG 2 cut(s) 71, 1937
BfuI GTATCC 1 cut(s) 646
BisI GCNGC 4 cut(s) 693, 696, 806, 848
BlpI GCTNAGC 3 cut(s) 626, 633, 1029
BlsI GCNGC 4 cut(s) 694, 697, 807, 849
BmcAI AGTACT 1 cut(s) 1248
Bme1390I CCNGG 4 cut(s) 274, 714, 1712, 1843
Bme18I GGWCC 4 cut(s) 545, 677, 1261, 1582
BmgT120I GGNCC 6 cut(s) 545, 616, 677, 1261, 1582, 1707
BmiI GGNNCC 6 cut(s) 310, 718, 1118, 1262, 1263, 1709
BmrFI CCNGG 4 cut(s) 274, 714, 1712, 1843
BmsI GCATC 6 cut(s) 108, 247, 1136, 1284, 1629, 1923
BpiI GAAGAC 4 cut(s) 380, 652, 1158, 1716
BplI GAGNNNNNCTC 2 cut(s) 467, 499
BpmI CTGGAG 1 cut(s) 1749
Bpu10I CCTNAGC 1 cut(s) 576
Bpu1102I GCTNAGC 3 cut(s) 626, 633, 1029
Bpu14I TTCGAA 2 cut(s) 1170, 1407
BpuEI CTTGAG 1 cut(s) 1993
BpuMI CCSGG 1 cut(s) 1843
BsaAI YACGTR 2 cut(s) 1762, 1776
BsaJI CCNNGG 7 cut(s) 424, 564, 1017, 1120, 1710, 1711, 1842
BsaWI WCCGGW 2 cut(s) 229, 491
Bsc4I CCNNNNNNNGG 6 cut(s) 318, 1040, 1394, 1717, 1842, 1843
Bse1I ACTGG 6 cut(s) 327, 385, 679, 919, 982, 1535
Bse21I CCTNAGG 1 cut(s) 1586
Bse3DI GCAATG 2 cut(s) 438, 830
BseAI TCCGGA 1 cut(s) 229
BseBI CCWGG 3 cut(s) 274, 714, 1712
BseDI CCNNGG 7 cut(s) 424, 564, 1017, 1120, 1710, 1711, 1842
BseGI GGATG 4 cut(s) 292, 913, 937, 976
BseLI CCNNNNNNNGG 6 cut(s) 318, 1040, 1394, 1717, 1842, 1843
BseMI GCAATG 2 cut(s) 438, 830
BseMII CTCAG 9 cut(s) 435, 465, 498, 590, 624, 640, 1020, 1577, 1784
BseNI ACTGG 6 cut(s) 327, 385, 679, 919, 982, 1535
BseRI GAGGAG 2 cut(s) 424, 1427
BseSI GKGCMC 1 cut(s) 313
BseXI GCAGC 3 cut(s) 679, 682, 834
Bsh1236I CGCG 2 cut(s) 357, 1111
Bsh1285I CGRYCG 2 cut(s) 457, 1331
BshFI GGCC 3 cut(s) 384, 617, 1708
BshNI GGYRCC 2 cut(s) 308, 716
BsiEI CGRYCG 2 cut(s) 457, 1331
BsiHKAI GWGCWC 2 cut(s) 287, 499
BsiSI CCGG 3 cut(s) 230, 492, 1842
BslFI GGGAC 4 cut(s) 98, 925, 1247, 1370
BslI CCNNNNNNNGG 6 cut(s) 318, 1040, 1394, 1717, 1842, 1843
BsmAI GTCTC 3 cut(s) 154, 470, 1603
BsmBI CGTCTC 1 cut(s) 154
BsmFI GGGAC 4 cut(s) 98, 925, 1247, 1370
BsnI GGCC 3 cut(s) 384, 617, 1708
Bsp119I TTCGAA 2 cut(s) 1170, 1407
Bsp1286I GDGCHC 3 cut(s) 287, 313, 499
Bsp13I TCCGGA 1 cut(s) 229
Bsp143I GATC 9 cut(s) 276, 345, 1062, 1328, 1459, 1521, 1621, 1654, 1678
Bsp1720I GCTNAGC 3 cut(s) 626, 633, 1029
Bsp68I TCGCGA 1 cut(s) 1111
BspACI CCGC 3 cut(s) 705, 805, 1089
BspANI GGCC 3 cut(s) 384, 617, 1708
BspCNI CTCAG 9 cut(s) 436, 466, 499, 589, 625, 639, 1021, 1578, 1785
BspEI TCCGGA 1 cut(s) 229
BspFNI CGCG 2 cut(s) 357, 1111
BspLI GGNNCC 6 cut(s) 310, 718, 1118, 1262, 1263, 1709
BspPI GGATC 5 cut(s) 284, 353, 1454, 1662, 1673
BspQI GCTCTTC 1 cut(s) 28
BspT104I TTCGAA 2 cut(s) 1170, 1407
BspT107I GGYRCC 2 cut(s) 308, 716
BspTI CTTAAG 2 cut(s) 71, 1937
BsrDI GCAATG 2 cut(s) 438, 830
BsrI ACTGG 6 cut(s) 327, 385, 679, 919, 982, 1535
BssECI CCNNGG 7 cut(s) 424, 564, 1017, 1120, 1710, 1711, 1842
BssMI GATC 9 cut(s) 276, 345, 1062, 1328, 1459, 1521, 1621, 1654, 1678
BssSI CACGAG 1 cut(s) 582
BssT1I CCWWGG 2 cut(s) 1017, 1120
Bst2BI CACGAG 1 cut(s) 582
Bst2UI CCWGG 3 cut(s) 274, 714, 1712
Bst4CI ACNGT 7 cut(s) 223, 301, 425, 565, 605, 658, 1437
Bst6I CTCTTC 2 cut(s) 28, 1404
BstAFI CTTAAG 2 cut(s) 71, 1937
BstBAI YACGTR 2 cut(s) 1762, 1776
BstBI TTCGAA 2 cut(s) 1170, 1407
BstC8I GCNNGC 1 cut(s) 852
BstDSI CCRYGG 2 cut(s) 424, 564
BstENI CCTNNNNNAGG 1 cut(s) 1715
BstF5I GGATG 4 cut(s) 292, 913, 937, 976
BstFNI CGCG 2 cut(s) 357, 1111
BstH2I RGCGCY 1 cut(s) 633
BstHHI GCGC 3 cut(s) 357, 632, 639
BstKTI GATC 9 cut(s) 279, 348, 1065, 1331, 1462, 1524, 1624, 1657, 1681
BstMAI GTCTC 3 cut(s) 154, 470, 1603
BstMBI GATC 9 cut(s) 276, 345, 1062, 1328, 1459, 1521, 1621, 1654, 1678
BstMCI CGRYCG 2 cut(s) 457, 1331
BstMWI GCNNNNNNNGC 5 cut(s) 127, 614, 636, 692, 1133
BstNI CCWGG 3 cut(s) 274, 714, 1712
BstNSI RCATGY 1 cut(s) 898
BstSCI CCNGG 4 cut(s) 272, 712, 1710, 1841
BstSFI CTRYAG 1 cut(s) 1131
BstSLI GKGCMC 1 cut(s) 313
BstSNI TACGTA 1 cut(s) 1776
BstUI CGCG 2 cut(s) 357, 1111
BstV1I GCAGC 3 cut(s) 679, 682, 834
BstV2I GAAGAC 4 cut(s) 380, 652, 1158, 1716
BstX2I RGATCY 4 cut(s) 345, 1459, 1654, 1678
BstXI CCANNNNNNTGG 1 cut(s) 304
BstYI RGATCY 4 cut(s) 345, 1459, 1654, 1678
Bsu36I CCTNAGG 1 cut(s) 1586
BsuI GTATCC 1 cut(s) 646
BsuRI GGCC 3 cut(s) 384, 617, 1708
BtgI CCRYGG 2 cut(s) 424, 564
BtsCI GGATG 4 cut(s) 292, 913, 937, 976
BtsI GCAGTG 1 cut(s) 409
BtsIMutI CAGTG 6 cut(s) 297, 378, 409, 610, 1542, 1790
BtuMI TCGCGA 1 cut(s) 1111
Cac8I GCNNGC 1 cut(s) 852
CfoI GCGC 3 cut(s) 357, 632, 639
Cfr13I GGNCC 6 cut(s) 545, 616, 677, 1261, 1582, 1707
Csp6I GTAC 2 cut(s) 717, 1247
CviAII CATG 8 cut(s) 243, 342, 888, 895, 1177, 1499, 1563, 1995
CviQI GTAC 2 cut(s) 717, 1247
DpnI GATC 9 cut(s) 278, 347, 1064, 1330, 1461, 1523, 1623, 1656, 1680
DpnII GATC 9 cut(s) 276, 345, 1062, 1328, 1459, 1521, 1621, 1654, 1678
EaeI YGGCCR 1 cut(s) 382
Eam1104I CTCTTC 2 cut(s) 28, 1404
EarI CTCTTC 2 cut(s) 28, 1404
Ecl136II GAGCTC 1 cut(s) 285
Eco105I TACGTA 1 cut(s) 1776
Eco130I CCWWGG 2 cut(s) 1017, 1120
Eco24I GRGCYC 1 cut(s) 287
Eco47I GGWCC 4 cut(s) 545, 677, 1261, 1582
Eco47III AGCGCT 1 cut(s) 631
Eco53kI GAGCTC 1 cut(s) 285
Eco57I CTGAAG 1 cut(s) 1251
Eco81I CCTNAGG 1 cut(s) 1586
EcoICRI GAGCTC 1 cut(s) 285
EcoNI CCTNNNNNAGG 1 cut(s) 1715
EcoO109I RGGNCCY 2 cut(s) 616, 1261
EcoRI GAATTC 1 cut(s) 1105
EcoRII CCWGG 3 cut(s) 272, 712, 1710
EcoT14I CCWWGG 2 cut(s) 1017, 1120
EcoT38I GRGCYC 1 cut(s) 287
ErhI CCWWGG 2 cut(s) 1017, 1120
Esp3I CGTCTC 1 cut(s) 154
FaeI CATG 8 cut(s) 246, 345, 891, 898, 1180, 1502, 1566, 1998
FalI AAGNNNNNCTT 4 cut(s) 794, 826, 857, 889
FaqI GGGAC 4 cut(s) 98, 925, 1247, 1370
FatI CATG 8 cut(s) 242, 341, 887, 894, 1176, 1498, 1562, 1994
FauNDI CATATG 1 cut(s) 553
FbaI TGATCA 1 cut(s) 1062
Fnu4HI GCNGC 4 cut(s) 693, 696, 806, 848
FokI GGATG 4 cut(s) 279, 920, 924, 983
FriOI GRGCYC 1 cut(s) 287
Fsp4HI GCNGC 4 cut(s) 693, 696, 806, 848
FspBI CTAG 7 cut(s) 168, 771, 777, 1055, 1071, 1233, 1676
GlaI GCGC 3 cut(s) 356, 631, 638
GluI GCNGC 4 cut(s) 693, 696, 806, 848
GsuI CTGGAG 1 cut(s) 1749
HaeII RGCGCY 1 cut(s) 633
HaeIII GGCC 3 cut(s) 384, 617, 1708
HapII CCGG 3 cut(s) 230, 492, 1842
HhaI GCGC 3 cut(s) 357, 632, 639
Hin1II CATG 8 cut(s) 246, 345, 891, 898, 1180, 1502, 1566, 1998
Hin6I GCGC 3 cut(s) 355, 630, 637
HinP1I GCGC 3 cut(s) 355, 630, 637
HindIII AAGCTT 1 cut(s) 1848
HpaII CCGG 3 cut(s) 230, 492, 1842
HphI GGTGA 3 cut(s) 440, 1103, 1108
Hpy166II GTNNAC 2 cut(s) 378, 1375
Hpy188I TCNGA 8 cut(s) 508, 514, 667, 823, 844, 1289, 1444, 1626
Hpy8I GTNNAC 2 cut(s) 378, 1375
HpyCH4III ACNGT 7 cut(s) 223, 301, 425, 565, 605, 658, 1437
HpyCH4IV ACGT 4 cut(s) 748, 1761, 1775, 1881
HpyF10VI GCNNNNNNNGC 5 cut(s) 127, 614, 636, 692, 1133
HpySE526I ACGT 4 cut(s) 748, 1761, 1775, 1881
Hsp92II CATG 8 cut(s) 246, 345, 891, 898, 1180, 1502, 1566, 1998
HspAI GCGC 3 cut(s) 355, 630, 637
KflI GGGWCCC 1 cut(s) 1261
Kpn2I TCCGGA 1 cut(s) 229
KpnI GGTACC 1 cut(s) 720
Ksp22I TGATCA 1 cut(s) 1062
Kzo9I GATC 9 cut(s) 276, 345, 1062, 1328, 1459, 1521, 1621, 1654, 1678
LguI GCTCTTC 1 cut(s) 28
LmnI GCTCC 2 cut(s) 502, 1116
Lsp1109I GCAGC 3 cut(s) 679, 682, 834
LweI GCATC 6 cut(s) 108, 247, 1136, 1284, 1629, 1923
MaeI CTAG 7 cut(s) 168, 771, 777, 1055, 1071, 1233, 1676
MaeII ACGT 4 cut(s) 748, 1761, 1775, 1881
MaeIII GTNAC 5 cut(s) 428, 890, 1012, 1615, 1893
MalI GATC 9 cut(s) 278, 347, 1064, 1330, 1461, 1523, 1623, 1656, 1680
MboI GATC 9 cut(s) 276, 345, 1062, 1328, 1459, 1521, 1621, 1654, 1678
MfeI CAATTG 2 cut(s) 463, 860
MflI RGATCY 4 cut(s) 345, 1459, 1654, 1678
MhlI GDGCHC 3 cut(s) 287, 313, 499
MlyI GAGTC 8 cut(s) 425, 472, 518, 1031, 1462, 1605, 1730, 1873
MmeI TCCRAC 2 cut(s) 818, 1487
MroI TCCGGA 1 cut(s) 229
MseI TTAA 7 cut(s) 72, 78, 875, 999, 1662, 1938, 1991
MslI CAYNNNNRTG 2 cut(s) 426, 949
MspCI CTTAAG 2 cut(s) 71, 1937
MspI CCGG 3 cut(s) 230, 492, 1842
MspR9I CCNGG 4 cut(s) 274, 714, 1712, 1843
MunI CAATTG 2 cut(s) 463, 860
MvaI CCWGG 3 cut(s) 274, 714, 1712
MvnI CGCG 2 cut(s) 357, 1111
MwoI GCNNNNNNNGC 5 cut(s) 127, 614, 636, 692, 1133
NciI CCSGG 1 cut(s) 1843
NdeI CATATG 1 cut(s) 553
NdeII GATC 9 cut(s) 276, 345, 1062, 1328, 1459, 1521, 1621, 1654, 1678
NlaIII CATG 8 cut(s) 246, 345, 891, 898, 1180, 1502, 1566, 1998
NlaIV GGNNCC 6 cut(s) 310, 718, 1118, 1262, 1263, 1709
NmuCI GTSAC 1 cut(s) 428
NruI TCGCGA 1 cut(s) 1111
NspI RCATGY 1 cut(s) 898
NspV TTCGAA 2 cut(s) 1170, 1407
OliI CACNNNNGTG 1 cut(s) 426
PasI CCCWGGG 1 cut(s) 1711
PciI ACATGT 1 cut(s) 894
PciSI GCTCTTC 1 cut(s) 28
PcsI WCGNNNNNNNCGW 1 cut(s) 1324
PfeI GAWTC 8 cut(s) 142, 226, 247, 818, 1280, 1445, 1576, 1672
PflMI CCANNNNNTGG 1 cut(s) 1394
PfoI TCCNGGA 1 cut(s) 272
PkrI GCNGC 4 cut(s) 694, 697, 807, 849
Ple19I CGATCG 1 cut(s) 1331
PleI GAGTC 8 cut(s) 425, 472, 517, 1030, 1461, 1605, 1729, 1872
PpsI GAGTC 8 cut(s) 425, 472, 517, 1030, 1461, 1605, 1729, 1872
Ppu21I YACGTR 2 cut(s) 1762, 1776
PpuMI RGGWCCY 1 cut(s) 1261
PscI ACATGT 1 cut(s) 894
Psp124BI GAGCTC 1 cut(s) 287
Psp5II RGGWCCY 1 cut(s) 1261
Psp6I CCWGG 3 cut(s) 272, 712, 1710
PspGI CCWGG 3 cut(s) 272, 712, 1710
PspN4I GGNNCC 6 cut(s) 310, 718, 1118, 1262, 1263, 1709
PspPI GGNCC 6 cut(s) 545, 616, 677, 1261, 1582, 1707
PspPPI RGGWCCY 1 cut(s) 1261
PsuI RGATCY 4 cut(s) 345, 1459, 1654, 1678
PvuI CGATCG 1 cut(s) 1331
RruI TCGCGA 1 cut(s) 1111
RsaI GTAC 2 cut(s) 718, 1248
RsaNI GTAC 2 cut(s) 717, 1247
RseI CAYNNNNRTG 2 cut(s) 426, 949
SacI GAGCTC 1 cut(s) 287
SapI GCTCTTC 1 cut(s) 28
SaqAI TTAA 7 cut(s) 72, 78, 875, 999, 1662, 1938, 1991
SatI GCNGC 4 cut(s) 693, 696, 806, 848
Sau3AI GATC 9 cut(s) 276, 345, 1062, 1328, 1459, 1521, 1621, 1654, 1678
Sau96I GGNCC 6 cut(s) 545, 616, 677, 1261, 1582, 1707
ScaI AGTACT 1 cut(s) 1248
SchI GAGTC 8 cut(s) 425, 472, 518, 1031, 1462, 1605, 1730, 1873
ScrFI CCNGG 4 cut(s) 274, 714, 1712, 1843
SduI GDGCHC 3 cut(s) 287, 313, 499
SfaNI GCATC 6 cut(s) 108, 247, 1136, 1284, 1629, 1923
SfcI CTRYAG 1 cut(s) 1131
SfuI TTCGAA 2 cut(s) 1170, 1407
SinI GGWCC 4 cut(s) 545, 677, 1261, 1582
SmiMI CAYNNNNRTG 2 cut(s) 426, 949
SmlI CTYRAG 3 cut(s) 71, 1937, 1972
SmoI CTYRAG 3 cut(s) 71, 1937, 1972
SnaBI TACGTA 1 cut(s) 1776
SpeI ACTAGT 1 cut(s) 1070
SsiI CCGC 3 cut(s) 705, 805, 1089
SspMI CTAG 7 cut(s) 168, 771, 777, 1055, 1071, 1233, 1676
SstI GAGCTC 1 cut(s) 287
StyD4I CCNGG 4 cut(s) 272, 712, 1710, 1841
StyI CCWWGG 2 cut(s) 1017, 1120
TaaI ACNGT 7 cut(s) 223, 301, 425, 565, 605, 658, 1437
TaiI ACGT 4 cut(s) 751, 1764, 1778, 1884
TaqI TCGA 4 cut(s) 1170, 1278, 1407, 1911
TaqII GACCGA 2 cut(s) 443, 1833
TatI WGTACW 1 cut(s) 1246
TauI GCSGC 1 cut(s) 808
TfiI GAWTC 8 cut(s) 142, 226, 247, 818, 1280, 1445, 1576, 1672
Tru1I TTAA 7 cut(s) 72, 78, 875, 999, 1662, 1938, 1991
Tru9I TTAA 7 cut(s) 72, 78, 875, 999, 1662, 1938, 1991
TscAI CASTG 6 cut(s) 304, 385, 409, 610, 1542, 1797
TseFI GTSAC 1 cut(s) 428
TseI GCWGC 3 cut(s) 692, 695, 847
Tsp45I GTSAC 1 cut(s) 428
TspGWI ACGGA 1 cut(s) 1667
TspRI CASTG 6 cut(s) 304, 385, 409, 610, 1542, 1797
Van91I CCANNNNNTGG 1 cut(s) 1394
Vha464I CTTAAG 2 cut(s) 71, 1937
VpaK11BI GGWCC 4 cut(s) 545, 677, 1261, 1582
XagI CCTNNNNNAGG 1 cut(s) 1715
XapI RAATTY 4 cut(s) 1049, 1105, 1294, 1783
XbaI TCTAGA 2 cut(s) 776, 1675
XceI RCATGY 1 cut(s) 898
XspI CTAG 7 cut(s) 168, 771, 777, 1055, 1071, 1233, 1676
ZrmI AGTACT 1 cut(s) 1248
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.