MD13G1107300.v1.1

Very-long-chain 3-oxoacyl-CoA reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0001251
Physical Location & Seq
Reverse (-)
7682548 .. 7683336
789 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1107300.v1.1.491

Sequence Viewer

Length: 468 bp
ATGGAATTCCAACAATTTTTCCTAGTGGCAGTAAGCACTCTAGGGTTCATCTCTCTTTGCAAATCATTCATCAATTTTCTCACATGGGTCTGGCTCTTGTTCCTCAGACCCCTAAAGAATCTCAAGGAGTATGGCTCGTGGGCTCTCATCACTGGGCCCACTGACGGGATTGAAAAATCCCTAGCCTTCGAAATGGCTTCAAAGGATCTTAATGTAGTTTTAGAGGTTGATTTGGAGCTGATGGAGAGCATTGTGAAGGTGAACATGGAGGGTGCCACGTGGATCACTAGGGCAGTGCTTCCAGTTATGCTGAGGAAGAAGAAGGGAGCAATTGTTAACATTGGTTCTGCTTCCTCTGAGACACTCCTTTCTTACCCTATTTACACTATTTATGCTGCATCTAAAACGGTGATGAACGGAGTGACAGTCACACTTAAAAGTTATTCTCTTTCGGGAATGCCCAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00062 ko00140 ko01040 ko01100 ko01110 ko01212 map00062 map00140 map01040 map01100 map01110 map01212
Pfam Domains
Protein Families
adh_short

Protein Analysis

156

Amino Acids

17.24

Weight (kDa)

8.66

Isoelectric Point (pI)

18.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
adh_short_C2 PF13561 55 - 142 2.2e-08 Enoyl-(Acyl carrier protein) reductase
adh_short PF00106 74 - 146 2.3e-13 short chain dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 272
AclWI GGATC 2 cut(s) 213, 290
AcsI RAATTY 1 cut(s) 5
AcvI CACGTG 1 cut(s) 279
AfiI CCNNNNNNNGG 2 cut(s) 164, 165
AgsI TTSAA 2 cut(s) 173, 201
AluBI AGCT 1 cut(s) 238
AluI AGCT 1 cut(s) 238
Alw26I GTCTC 1 cut(s) 353
AlwI GGATC 2 cut(s) 213, 290
AoxI GGCC 1 cut(s) 155
ApaI GGGCCC 1 cut(s) 159
ApeKI GCWGC 1 cut(s) 395
ApoI RAATTY 1 cut(s) 5
AspS9I GGNCC 2 cut(s) 155, 156
AsuHPI GGTGA 2 cut(s) 271, 421
AsuII TTCGAA 1 cut(s) 189
BaeGI GKGCMC 1 cut(s) 159
BanI GGYRCC 1 cut(s) 272
BanII GRGCYC 2 cut(s) 145, 159
BauI CACGAG 1 cut(s) 136
BbrPI CACGTG 1 cut(s) 279
BbvCI CCTCAGC 1 cut(s) 311
BbvI GCAGC 1 cut(s) 382
BccI CCATC 1 cut(s) 235
BcoDI GTCTC 1 cut(s) 353
BfaI CTAG 4 cut(s) 23, 41, 182, 288
BisI GCNGC 1 cut(s) 396
BlsI GCNGC 1 cut(s) 397
BmgT120I GGNCC 2 cut(s) 155, 156
BmiI GGNNCC 2 cut(s) 157, 274
BmrI ACTGGG 1 cut(s) 162
BmsI GCATC 1 cut(s) 407
BmuI ACTGGG 1 cut(s) 162
BplI GAGNNNNNCTC 2 cut(s) 119, 151
Bpu10I CCTNAGC 1 cut(s) 311
Bpu14I TTCGAA 1 cut(s) 189
BpuEI CTTGAG 1 cut(s) 107
BsaAI YACGTR 1 cut(s) 279
BsaXI ACNNNNNCTCC 4 cut(s) 318, 348, 411, 441
Bsc4I CCNNNNNNNGG 2 cut(s) 164, 165
Bse1I ACTGG 2 cut(s) 157, 302
BseLI CCNNNNNNNGG 2 cut(s) 164, 165
BseMII CTCAG 3 cut(s) 118, 302, 348
BseNI ACTGG 2 cut(s) 157, 302
BseSI GKGCMC 1 cut(s) 159
BseXI GCAGC 1 cut(s) 382
BshFI GGCC 1 cut(s) 157
BshNI GGYRCC 1 cut(s) 272
BslI CCNNNNNNNGG 2 cut(s) 164, 165
BsmAI GTCTC 1 cut(s) 353
BsmI GAATGC 1 cut(s) 462
BsnI GGCC 1 cut(s) 157
Bsp119I TTCGAA 1 cut(s) 189
Bsp120I GGGCCC 1 cut(s) 155
Bsp1286I GDGCHC 2 cut(s) 145, 159
Bsp143I GATC 2 cut(s) 205, 282
BspANI GGCC 1 cut(s) 157
BspCNI CTCAG 3 cut(s) 117, 303, 349
BspLI GGNNCC 2 cut(s) 157, 274
BspPI GGATC 2 cut(s) 213, 290
BspT104I TTCGAA 1 cut(s) 189
BspT107I GGYRCC 1 cut(s) 272
BsrI ACTGG 2 cut(s) 157, 302
BssMI GATC 2 cut(s) 205, 282
BssSI CACGAG 1 cut(s) 136
Bst2BI CACGAG 1 cut(s) 136
Bst4CI ACNGT 2 cut(s) 409, 427
BstBAI YACGTR 1 cut(s) 279
BstBI TTCGAA 1 cut(s) 189
BstDEI CTNAG 3 cut(s) 104, 311, 357
BstKTI GATC 2 cut(s) 208, 285
BstMAI GTCTC 1 cut(s) 353
BstMBI GATC 2 cut(s) 205, 282
BstSLI GKGCMC 1 cut(s) 159
BstV1I GCAGC 1 cut(s) 382
BstX2I RGATCY 1 cut(s) 205
BstYI RGATCY 1 cut(s) 205
BsuRI GGCC 1 cut(s) 157
BtsI GCAGTG 1 cut(s) 300
BtsIMutI CAGTG 3 cut(s) 150, 159, 300
Cfr13I GGNCC 2 cut(s) 155, 156
CviAII CATG 2 cut(s) 84, 265
CviJI RGCY 7 cut(s) 94, 135, 143, 157, 185, 197, 238
CviKI_1 RGCY 7 cut(s) 94, 135, 143, 157, 185, 197, 238
DdeI CTNAG 3 cut(s) 104, 311, 357
DpnI GATC 2 cut(s) 207, 284
DpnII GATC 2 cut(s) 205, 282
Eco24I GRGCYC 2 cut(s) 145, 159
Eco72I CACGTG 1 cut(s) 279
EcoRI GAATTC 1 cut(s) 5
EcoT38I GRGCYC 2 cut(s) 145, 159
FaeI CATG 2 cut(s) 87, 268
FaiI YATR 5 cut(s) 85, 132, 266, 308, 393
FatI CATG 2 cut(s) 83, 264
Fnu4HI GCNGC 1 cut(s) 396
FriOI GRGCYC 2 cut(s) 145, 159
Fsp4HI GCNGC 1 cut(s) 396
FspBI CTAG 4 cut(s) 23, 41, 182, 288
GluI GCNGC 1 cut(s) 396
HaeIII GGCC 1 cut(s) 157
Hin1II CATG 2 cut(s) 87, 268
HincII GTYRAC 1 cut(s) 337
HindII GTYRAC 1 cut(s) 337
HinfI GANTC 1 cut(s) 118
HpaI GTTAAC 1 cut(s) 337
HphI GGTGA 2 cut(s) 271, 421
Hpy166II GTNNAC 2 cut(s) 262, 337
Hpy188I TCNGA 2 cut(s) 107, 358
Hpy188III TCNNGA 1 cut(s) 453
Hpy8I GTNNAC 2 cut(s) 262, 337
HpyAV CCTTC 3 cut(s) 196, 250, 316
HpyCH4III ACNGT 2 cut(s) 409, 427
HpyCH4IV ACGT 1 cut(s) 278
HpyCH4V TGCA 2 cut(s) 60, 398
HpyF3I CTNAG 3 cut(s) 104, 311, 357
HpySE526I ACGT 1 cut(s) 278
Hsp92II CATG 2 cut(s) 87, 268
KspAI GTTAAC 1 cut(s) 337
Kzo9I GATC 2 cut(s) 205, 282
LmnI GCTCC 2 cut(s) 235, 326
LpnPI CCDG 3 cut(s) 76, 138, 315
Lsp1109I GCAGC 1 cut(s) 382
LweI GCATC 1 cut(s) 407
MaeI CTAG 4 cut(s) 23, 41, 182, 288
MaeII ACGT 1 cut(s) 278
MaeIII GTNAC 2 cut(s) 421, 427
MalI GATC 2 cut(s) 207, 284
MboI GATC 2 cut(s) 205, 282
MboII GAAGA 2 cut(s) 328, 331
MfeI CAATTG 1 cut(s) 330
MflI RGATCY 1 cut(s) 205
MhlI GDGCHC 2 cut(s) 145, 159
MluCI AATT 5 cut(s) 5, 14, 73, 330, 463
MmeI TCCRAC 1 cut(s) 34
MnlI CCTC 5 cut(s) 113, 217, 262, 306, 364
MseI TTAA 3 cut(s) 210, 336, 435
MunI CAATTG 1 cut(s) 330
Mva1269I GAATGC 1 cut(s) 462
NdeII GATC 2 cut(s) 205, 282
NlaIII CATG 2 cut(s) 87, 268
NlaIV GGNNCC 2 cut(s) 157, 274
NmuCI GTSAC 2 cut(s) 421, 427
NspV TTCGAA 1 cut(s) 189
PctI GAATGC 1 cut(s) 462
PfeI GAWTC 1 cut(s) 118
PkrI GCNGC 1 cut(s) 397
PmaCI CACGTG 1 cut(s) 279
PmlI CACGTG 1 cut(s) 279
Ppu21I YACGTR 1 cut(s) 279
PspCI CACGTG 1 cut(s) 279
PspN4I GGNNCC 2 cut(s) 157, 274
PspOMI GGGCCC 1 cut(s) 155
PspPI GGNCC 2 cut(s) 155, 156
PsuI RGATCY 1 cut(s) 205
SaqAI TTAA 3 cut(s) 210, 336, 435
SatI GCNGC 1 cut(s) 396
Sau3AI GATC 2 cut(s) 205, 282
Sau96I GGNCC 2 cut(s) 155, 156
SduI GDGCHC 2 cut(s) 145, 159
SetI ASST 4 cut(s) 228, 240, 261, 281
SfaNI GCATC 1 cut(s) 407
SfuI TTCGAA 1 cut(s) 189
SmlI CTYRAG 1 cut(s) 122
SmoI CTYRAG 1 cut(s) 122
Sse9I AATT 5 cut(s) 5, 14, 73, 330, 463
SspMI CTAG 4 cut(s) 23, 41, 182, 288
TaaI ACNGT 2 cut(s) 409, 427
TaiI ACGT 1 cut(s) 281
TaqI TCGA 1 cut(s) 189
TasI AATT 5 cut(s) 5, 14, 73, 330, 463
TfiI GAWTC 1 cut(s) 118
Tru1I TTAA 3 cut(s) 210, 336, 435
Tru9I TTAA 3 cut(s) 210, 336, 435
TscAI CASTG 3 cut(s) 157, 166, 300
TseFI GTSAC 2 cut(s) 421, 427
TseI GCWGC 1 cut(s) 395
Tsp45I GTSAC 2 cut(s) 421, 427
TspDTI ATGAA 3 cut(s) 37, 58, 428
TspGWI ACGGA 1 cut(s) 432
TspRI CASTG 3 cut(s) 157, 166, 300
XapI RAATTY 1 cut(s) 5
XspI CTAG 4 cut(s) 23, 41, 182, 288
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.