Rh4AG312200

Belongs to the short-chain dehydrogenases reductases (SDR) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0001251
Physical Location & Seq
Forward (+)
62306855 .. 62310392
3538 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG312200.1

Sequence Viewer

Length: 1167 bp
ATGTCCTTTTATACGTGCCAACATGCTTCTTTTAGTGAAAAATCTTCTACTTCAGTTCACTTGTTATTCACAGTGAAAACTTGCTTGAACTTCATTGCACCAAAAAAAAGAAGATATACTTGTAGGCTTGTAGCTAGTCAATTCTCTTTTGACCCTACTTACCGAATTGCTTTGCAGCTGATAACTGATTCAATAATATTGGCAATGGAATTGCAAGAAGCTTTCATCGTCACAGCAAGTTGTCTAGGCTTCATCTCTGTTTGTAAAGCTTTGGTCAATTTTTTGAGATGGGTGTGGGTCATGTTTCTGAGATCCCCCAAGAATCTCAAGGAGTATGGATCTTGGGCAATCATCACTGGCTCCACTGATGGAATTGGCAAAGCCCTTGCCTTTGAACTGGCTTCGAAGGGTCTCAACCTCGTCTTGGTTGGTCGAAACCCTTTGAAGCTCGAAGCTACCTCCAATGGAATCCGCGAGAAATACAGTCATAAACAAGTGGAAATTAAGAGCATTGTCGTCGACTTGGCCAAAGCTAGTGGGGAGGAAATAGCTAGGACCATAGAGGAAGGAATCAAAGGTCTTGATGTTGGCATTTTGATTAATAATGCAGGGATGTCTTACCCTTATGCAAGGTTCTTTCATGAGGTTGATTCGGAGCTGATGGAGAACATGTTAAAGGTGAACATGGAAGCCCCAACTTGGGTGACTAGGGCAGTGCTTCCAGGAATGCTCAAGAAGAAGAAAGGAGCAATTGTCAATATCGGCTCTGCTTCCTCCAAGCACCCTTCTCTGCCCCTCTACACACTATATGCTGCTACCAAATCGTACATTAAAATGTTCGCAAGATGCATTAATTTGGAATACAAGCAGCATGGAATTGATGTTCAGTGTCAGGTGCCTCATTTCGTGGCGACGAAGATGACAGAGGATTTGAAGAAGTCGGTTCCCTTGGTAGTGGCAACACTGGAGAGATTCACGAAAGCAAGCATTCGATGGATTGGTTATGAGCATCTATGTAATCCCTACTGGTTGCATTCAGTGGAGTGGTCCATAGTACATGCAGCTCCTGATGCATTAGTTAATGCCATCACGTTTTGGATCTGCAGAGGTATCAGAAAGAGAGGCCAAATGAAGGAGTACGTCACAGTAAAAAGATGCAGTGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00062 ko00140 ko01040 ko01100 ko01110 ko01212 map00062 map00140 map01040 map01100 map01110 map01212
Pfam Domains
Protein Families
adh_short

Protein Analysis

388

Amino Acids

43.71

Weight (kDa)

9.39

Isoelectric Point (pI)

32.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
adh_short PF00106 115 - 313 9.2e-42 short chain dehydrogenase
adh_short_C2 PF13561 122 - 314 1.2e-22 Enoyl-(Acyl carrier protein) reductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 895
AccI GTMKAC 1 cut(s) 519
AccII CGCG 1 cut(s) 474
AciI CCGC 1 cut(s) 472
AclWI GGATC 3 cut(s) 306, 346, 1106
AcoI YGGCCR 1 cut(s) 525
AcuI CTGAAG 1 cut(s) 36
AfaI GTAC 3 cut(s) 827, 1056, 1139
AfiI CCNNNNNNNGG 4 cut(s) 424, 699, 700, 1132
AflIII ACRYGT 1 cut(s) 669
AgsI TTSAA 5 cut(s) 88, 192, 395, 445, 934
AjnI CCWGG 1 cut(s) 721
AloI GAACNNNNNNTCC 2 cut(s) 867, 899
Alw26I GTCTC 1 cut(s) 416
AlwI GGATC 3 cut(s) 306, 346, 1106
AlwNI CAGNNNCTG 1 cut(s) 1067
AoxI GGCC 2 cut(s) 525, 1123
ApeKI GCWGC 4 cut(s) 175, 812, 868, 1061
ArsI GACNNNNNNTTYG 2 cut(s) 913, 945
AseI ATTAAT 2 cut(s) 600, 852
AspS9I GGNCC 2 cut(s) 555, 1047
AsuHPI GGTGA 2 cut(s) 691, 715
AsuII TTCGAA 1 cut(s) 404
AvaII GGWCC 2 cut(s) 555, 1047
BalI TGGCCA 1 cut(s) 527
BanI GGYRCC 1 cut(s) 895
BbvI GCAGC 4 cut(s) 187, 799, 880, 1073
BccI CCATC 5 cut(s) 282, 362, 655, 987, 1094
BcgI CGANNNNNNTGC 2 cut(s) 499, 533
BciT130I CCWGG 1 cut(s) 723
BcoDI GTCTC 1 cut(s) 416
BfaI CTAG 5 cut(s) 135, 245, 534, 552, 708
BfmI CTRYAG 1 cut(s) 1102
BisI GCNGC 4 cut(s) 176, 813, 869, 1062
BlsI GCNGC 4 cut(s) 177, 814, 870, 1063
Bme1390I CCNGG 1 cut(s) 723
Bme18I GGWCC 2 cut(s) 555, 1047
BmgT120I GGNCC 2 cut(s) 555, 1047
BmiI GGNNCC 3 cut(s) 361, 897, 945
BmrFI CCNGG 1 cut(s) 723
BmsI GCATC 4 cut(s) 836, 1018, 1060, 1145
BpmI CTGGAG 1 cut(s) 986
Bpu14I TTCGAA 1 cut(s) 404
BpuEI CTTGAG 2 cut(s) 311, 716
BsaAI YACGTR 1 cut(s) 15
BsaI GGTCTC 1 cut(s) 416
BsaJI CCNNGG 1 cut(s) 948
BsaXI ACNNNNNCTCC 4 cut(s) 656, 686, 738, 768
Bsc4I CCNNNNNNNGG 4 cut(s) 424, 699, 700, 1132
Bse1I ACTGG 4 cut(s) 361, 402, 969, 1031
Bse3DI GCAATG 2 cut(s) 93, 210
BseBI CCWGG 1 cut(s) 723
BseDI CCNNGG 1 cut(s) 948
BseGI GGATG 1 cut(s) 618
BseLI CCNNNNNNNGG 4 cut(s) 424, 699, 700, 1132
BseMI GCAATG 2 cut(s) 93, 210
BseMII CTCAG 1 cut(s) 299
BseNI ACTGG 4 cut(s) 361, 402, 969, 1031
BseXI GCAGC 4 cut(s) 187, 799, 880, 1073
Bsh1236I CGCG 1 cut(s) 474
BshFI GGCC 2 cut(s) 527, 1125
BshNI GGYRCC 1 cut(s) 895
BslI CCNNNNNNNGG 4 cut(s) 424, 699, 700, 1132
BsmAI GTCTC 1 cut(s) 416
BsmI GAATGC 3 cut(s) 732, 987, 1033
BsnI GGCC 2 cut(s) 527, 1125
Bso31I GGTCTC 1 cut(s) 416
Bsp119I TTCGAA 1 cut(s) 404
Bsp143I GATC 3 cut(s) 311, 338, 1098
BspACI CCGC 1 cut(s) 472
BspANI GGCC 2 cut(s) 527, 1125
BspCNI CTCAG 1 cut(s) 300
BspFNI CGCG 1 cut(s) 474
BspHI TCATGA 1 cut(s) 640
BspLI GGNNCC 3 cut(s) 361, 897, 945
BspMAI CTGCAG 1 cut(s) 1106
BspPI GGATC 3 cut(s) 306, 346, 1106
BspT104I TTCGAA 1 cut(s) 404
BspT107I GGYRCC 1 cut(s) 895
BspTNI GGTCTC 1 cut(s) 416
BsrDI GCAATG 2 cut(s) 93, 210
BsrI ACTGG 4 cut(s) 361, 402, 969, 1031
BssECI CCNNGG 1 cut(s) 948
BssMI GATC 3 cut(s) 311, 338, 1098
BssT1I CCWWGG 1 cut(s) 948
Bst2UI CCWGG 1 cut(s) 723
Bst4CI ACNGT 3 cut(s) 73, 485, 1147
BstBAI YACGTR 1 cut(s) 15
BstBI TTCGAA 1 cut(s) 404
BstC8I GCNNGC 1 cut(s) 985
BstDEI CTNAG 1 cut(s) 308
BstF5I GGATG 1 cut(s) 618
BstFNI CGCG 1 cut(s) 474
BstKTI GATC 3 cut(s) 314, 341, 1101
BstMAI GTCTC 1 cut(s) 416
BstMBI GATC 3 cut(s) 311, 338, 1098
BstMWI GCNNNNNNNGC 1 cut(s) 1070
BstNI CCWGG 1 cut(s) 723
BstNSI RCATGY 3 cut(s) 26, 673, 1061
BstSCI CCNGG 1 cut(s) 721
BstSFI CTRYAG 1 cut(s) 1102
BstUI CGCG 1 cut(s) 474
BstV1I GCAGC 4 cut(s) 187, 799, 880, 1073
BstX2I RGATCY 3 cut(s) 311, 338, 1098
BstYI RGATCY 3 cut(s) 311, 338, 1098
BsuRI GGCC 2 cut(s) 527, 1125
BtsCI GGATG 1 cut(s) 618
BtsI GCAGTG 2 cut(s) 720, 1165
BtsIMutI CAGTG 8 cut(s) 78, 354, 363, 720, 893, 962, 1044, 1165
Cac8I GCNNGC 1 cut(s) 985
CaiI CAGNNNCTG 1 cut(s) 1067
CciI TCATGA 1 cut(s) 640
Cfr13I GGNCC 2 cut(s) 555, 1047
Csp6I GTAC 3 cut(s) 826, 1055, 1138
CspCI CAANNNNNGTGG 2 cut(s) 517, 552
CviAII CATG 7 cut(s) 23, 301, 641, 670, 685, 872, 1058
CviQI GTAC 3 cut(s) 826, 1055, 1138
DdeI CTNAG 1 cut(s) 308
DpnI GATC 3 cut(s) 313, 340, 1100
DpnII GATC 3 cut(s) 311, 338, 1098
EaeI YGGCCR 1 cut(s) 525
Eco130I CCWWGG 1 cut(s) 948
Eco31I GGTCTC 1 cut(s) 416
Eco47I GGWCC 2 cut(s) 555, 1047
Eco57I CTGAAG 1 cut(s) 36
EcoRII CCWGG 1 cut(s) 721
EcoT14I CCWWGG 1 cut(s) 948
EcoT22I ATGCAT 2 cut(s) 851, 1075
ErhI CCWWGG 1 cut(s) 948
FaeI CATG 7 cut(s) 26, 304, 644, 673, 688, 875, 1061
FalI AAGNNNNNCTT 2 cut(s) 103, 135
FatI CATG 7 cut(s) 22, 300, 640, 669, 684, 871, 1057
FblI GTMKAC 1 cut(s) 519
Fnu4HI GCNGC 4 cut(s) 176, 813, 869, 1062
FokI GGATG 1 cut(s) 625
Fsp4HI GCNGC 4 cut(s) 176, 813, 869, 1062
FspBI CTAG 5 cut(s) 135, 245, 534, 552, 708
GluI GCNGC 4 cut(s) 176, 813, 869, 1062
GsuI CTGGAG 1 cut(s) 986
HaeIII GGCC 2 cut(s) 527, 1125
Hin1II CATG 7 cut(s) 26, 304, 644, 673, 688, 875, 1061
HincII GTYRAC 1 cut(s) 520
HindII GTYRAC 1 cut(s) 520
HindIII AAGCTT 2 cut(s) 219, 267
HinfI GANTC 6 cut(s) 188, 322, 468, 570, 650, 972
HphI GGTGA 2 cut(s) 691, 715
Hpy166II GTNNAC 3 cut(s) 58, 520, 682
Hpy188I TCNGA 3 cut(s) 309, 655, 1115
Hpy188III TCNNGA 5 cut(s) 581, 641, 733, 976, 1067
Hpy8I GTNNAC 3 cut(s) 58, 520, 682
Hpy99I CGWCG 2 cut(s) 521, 916
HpyAV CCTTC 4 cut(s) 400, 560, 795, 1126
HpyCH4III ACNGT 3 cut(s) 73, 485, 1147
HpyCH4IV ACGT 3 cut(s) 14, 1091, 1140
HpyF10VI GCNNNNNNNGC 1 cut(s) 1070
HpyF3I CTNAG 1 cut(s) 308
HpySE526I ACGT 3 cut(s) 14, 1091, 1140
Hsp92II CATG 7 cut(s) 26, 304, 644, 673, 688, 875, 1061
Kzo9I GATC 3 cut(s) 311, 338, 1098
LmnI GCTCC 4 cut(s) 365, 655, 746, 1069
LpnPI CCDG 9 cut(s) 342, 383, 594, 708, 735, 878, 950, 1012, 1080
Lsp1109I GCAGC 4 cut(s) 187, 799, 880, 1073
LweI GCATC 4 cut(s) 836, 1018, 1060, 1145
MaeI CTAG 5 cut(s) 135, 245, 534, 552, 708
MaeII ACGT 3 cut(s) 14, 1091, 1140
MaeIII GTNAC 3 cut(s) 229, 703, 1141
MalI GATC 3 cut(s) 313, 340, 1100
MboI GATC 3 cut(s) 311, 338, 1098
MboII GAAGA 6 cut(s) 36, 123, 748, 751, 928, 946
MfeI CAATTG 1 cut(s) 750
MflI RGATCY 3 cut(s) 311, 338, 1098
MlsI TGGCCA 1 cut(s) 527
MluCI AATT 9 cut(s) 140, 165, 209, 277, 372, 501, 750, 853, 876
MluNI TGGCCA 1 cut(s) 527
Mox20I TGGCCA 1 cut(s) 527
Mph1103I ATGCAT 2 cut(s) 851, 1075
MscI TGGCCA 1 cut(s) 527
MseI TTAA 7 cut(s) 504, 600, 674, 831, 852, 1080, 1165
MslI CAYNNNNRTG 1 cut(s) 833
Msp20I TGGCCA 1 cut(s) 527
MspA1I CMGCKG 1 cut(s) 178
MspR9I CCNGG 1 cut(s) 723
MunI CAATTG 1 cut(s) 750
Mva1269I GAATGC 3 cut(s) 732, 987, 1033
MvaI CCWGG 1 cut(s) 723
MvnI CGCG 1 cut(s) 474
MwoI GCNNNNNNNGC 1 cut(s) 1070
NdeII GATC 3 cut(s) 311, 338, 1098
NlaIII CATG 7 cut(s) 26, 304, 644, 673, 688, 875, 1061
NlaIV GGNNCC 3 cut(s) 361, 897, 945
NmuCI GTSAC 3 cut(s) 229, 703, 1141
NsiI ATGCAT 2 cut(s) 851, 1075
NspI RCATGY 3 cut(s) 26, 673, 1061
NspV TTCGAA 1 cut(s) 404
PagI TCATGA 1 cut(s) 640
PciI ACATGT 1 cut(s) 669
PctI GAATGC 3 cut(s) 732, 987, 1033
PfeI GAWTC 6 cut(s) 188, 322, 468, 570, 650, 972
PfoI TCCNGGA 1 cut(s) 721
PkrI GCNGC 4 cut(s) 177, 814, 870, 1063
Ppu21I YACGTR 1 cut(s) 15
PscI ACATGT 1 cut(s) 669
PshBI ATTAAT 2 cut(s) 600, 852
Psp6I CCWGG 1 cut(s) 721
PspGI CCWGG 1 cut(s) 721
PspN4I GGNNCC 3 cut(s) 361, 897, 945
PspPI GGNCC 2 cut(s) 555, 1047
PstI CTGCAG 1 cut(s) 1106
PstNI CAGNNNCTG 1 cut(s) 1067
PsuI RGATCY 3 cut(s) 311, 338, 1098
PvuII CAGCTG 1 cut(s) 178
RsaI GTAC 3 cut(s) 827, 1056, 1139
RsaNI GTAC 3 cut(s) 826, 1055, 1138
RseI CAYNNNNRTG 1 cut(s) 833
SalI GTCGAC 1 cut(s) 518
SaqAI TTAA 7 cut(s) 504, 600, 674, 831, 852, 1080, 1165
SatI GCNGC 4 cut(s) 176, 813, 869, 1062
Sau3AI GATC 3 cut(s) 311, 338, 1098
Sau96I GGNCC 2 cut(s) 555, 1047
ScrFI CCNGG 1 cut(s) 723
SfaNI GCATC 4 cut(s) 836, 1018, 1060, 1145
SfcI CTRYAG 1 cut(s) 1102
SfuI TTCGAA 1 cut(s) 404
SinI GGWCC 2 cut(s) 555, 1047
SmiMI CAYNNNNRTG 1 cut(s) 833
SmlI CTYRAG 2 cut(s) 326, 731
SmoI CTYRAG 2 cut(s) 326, 731
Sse9I AATT 9 cut(s) 140, 165, 209, 277, 372, 501, 750, 853, 876
SsiI CCGC 1 cut(s) 472
SspI AATATT 1 cut(s) 198
SspMI CTAG 5 cut(s) 135, 245, 534, 552, 708
StyD4I CCNGG 1 cut(s) 721
StyI CCWWGG 1 cut(s) 948
TaaI ACNGT 3 cut(s) 73, 485, 1147
TaiI ACGT 3 cut(s) 17, 1094, 1143
TaqI TCGA 5 cut(s) 404, 433, 450, 519, 991
TasI AATT 9 cut(s) 140, 165, 209, 277, 372, 501, 750, 853, 876
TatI WGTACW 1 cut(s) 1054
TfiI GAWTC 6 cut(s) 188, 322, 468, 570, 650, 972
Tru1I TTAA 7 cut(s) 504, 600, 674, 831, 852, 1080, 1165
Tru9I TTAA 7 cut(s) 504, 600, 674, 831, 852, 1080, 1165
TscAI CASTG 8 cut(s) 78, 361, 370, 720, 893, 969, 1044, 1165
TseFI GTSAC 3 cut(s) 229, 703, 1141
TseI GCWGC 4 cut(s) 175, 812, 868, 1061
Tsp45I GTSAC 3 cut(s) 229, 703, 1141
TspDTI ATGAA 5 cut(s) 82, 214, 241, 629, 1145
TspRI CASTG 8 cut(s) 78, 361, 370, 720, 893, 969, 1044, 1165
VpaK11BI GGWCC 2 cut(s) 555, 1047
VspI ATTAAT 2 cut(s) 600, 852
XceI RCATGY 3 cut(s) 26, 673, 1061
XmiI GTMKAC 1 cut(s) 519
XspI CTAG 5 cut(s) 135, 245, 534, 552, 708
Zsp2I ATGCAT 2 cut(s) 851, 1075
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.