Rorug04G0254900

Belongs to the short-chain dehydrogenases reductases (SDR) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0001251
Physical Location & Seq
Forward (+)
42765358 .. 42765909
552 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0254900.1

Sequence Viewer

Length: 504 bp
ATGGTGCACACTCCCCTTGAGACAGTAGCAGACCTCATTGATCCTACATCTAGAACCTGGATTTCCACCTTGGTTCATACTTTATTTAGCCCTCAGGTGGCTGCCAGTATTTTGTGCATTCCATTGCGACATCATCCTATTCCTGATAGAAGATGTTGGACCCCTGAAAAGAGAGGGTTATTTACGGTGAAAAGTGCCTACTGGATCGCTAGGCAGCAGATGCTATGGAAATGTATTTGGAAGGCCAAGATACCAGGGAAAGTTCAAATATGTTGCTGGAGGGCAGTTAATAATTTGCTACCTACAAGGGAACAACTTTCGAAGAGAGGTTATGATGGAGAAATGGGATGCTTACTCTGCAATTACAGAATGGAGAGCACTGATCATCTGTTTTGCAAATGTCCTACTGCTACGTCATTACTTTCAAGTACCCCGTTCTTGTTACAGTCCTCTATTCTACCCAACTTGGACTTTAAGGAATGGATGTTAGAACGAGCTCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00062 ko00140 ko01040 ko01100 ko01110 ko01212 map00062 map00140 map01040 map01100 map01110 map01212
Pfam Domains
Protein Families
adh_short

Protein Analysis

167

Amino Acids

19.27

Weight (kDa)

9.05

Isoelectric Point (pI)

39.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RVT PF13966 61 - 139 8.4e-20 zinc-binding in reverse transcriptase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 2 cut(s) 35, 212
AfaI GTAC 1 cut(s) 430
AfiI CCNNNNNNNGG 1 cut(s) 97
AgsI TTSAA 2 cut(s) 266, 426
AjnI CCWGG 2 cut(s) 56, 253
AluBI AGCT 1 cut(s) 497
AluI AGCT 1 cut(s) 497
Alw21I GWGCWC 3 cut(s) 9, 380, 499
Alw26I GTCTC 1 cut(s) 14
Alw44I GTGCAC 1 cut(s) 5
AlwI GGATC 2 cut(s) 35, 212
AoxI GGCC 1 cut(s) 243
ApaLI GTGCAC 1 cut(s) 5
ApeKI GCWGC 2 cut(s) 101, 214
AspS9I GGNCC 1 cut(s) 159
AsuHPI GGTGA 1 cut(s) 199
AsuII TTCGAA 1 cut(s) 320
AvaII GGWCC 1 cut(s) 159
AxyI CCTNAGG 1 cut(s) 93
BaeGI GKGCMC 1 cut(s) 9
BanII GRGCYC 1 cut(s) 499
Bbv12I GWGCWC 3 cut(s) 9, 380, 499
BbvI GCAGC 2 cut(s) 88, 226
BccI CCATC 1 cut(s) 329
BciT130I CCWGG 2 cut(s) 58, 255
BclI TGATCA 1 cut(s) 382
BcoDI GTCTC 1 cut(s) 14
BfaI CTAG 2 cut(s) 51, 210
BisI GCNGC 2 cut(s) 102, 215
BlsI GCNGC 2 cut(s) 103, 216
Bme1390I CCNGG 2 cut(s) 58, 255
Bme18I GGWCC 1 cut(s) 159
BmgT120I GGNCC 1 cut(s) 159
BmiI GGNNCC 1 cut(s) 161
BmrFI CCNGG 2 cut(s) 58, 255
BmsI GCATC 2 cut(s) 210, 338
BpmI CTGGAG 1 cut(s) 298
Bpu14I TTCGAA 1 cut(s) 320
BpuEI CTTGAG 1 cut(s) 38
BsaJI CCNNGG 2 cut(s) 69, 254
Bsc4I CCNNNNNNNGG 1 cut(s) 97
Bse1I ACTGG 2 cut(s) 105, 206
Bse21I CCTNAGG 1 cut(s) 93
Bse3DI GCAATG 1 cut(s) 122
BseBI CCWGG 2 cut(s) 58, 255
BseDI CCNNGG 2 cut(s) 69, 254
BseGI GGATG 3 cut(s) 133, 353, 489
BseLI CCNNNNNNNGG 1 cut(s) 97
BseMI GCAATG 1 cut(s) 122
BseMII CTCAG 1 cut(s) 107
BseNI ACTGG 2 cut(s) 105, 206
BseSI GKGCMC 1 cut(s) 9
BseXI GCAGC 2 cut(s) 88, 226
BshFI GGCC 1 cut(s) 245
BsiHKAI GWGCWC 3 cut(s) 9, 380, 499
BslI CCNNNNNNNGG 1 cut(s) 97
BsmAI GTCTC 1 cut(s) 14
BsmI GAATGC 1 cut(s) 117
BsnI GGCC 1 cut(s) 245
Bsp119I TTCGAA 1 cut(s) 320
Bsp1286I GDGCHC 3 cut(s) 9, 380, 499
Bsp143I GATC 3 cut(s) 40, 204, 382
BspANI GGCC 1 cut(s) 245
BspCNI CTCAG 1 cut(s) 106
BspLI GGNNCC 1 cut(s) 161
BspPI GGATC 2 cut(s) 35, 212
BspT104I TTCGAA 1 cut(s) 320
BsrDI GCAATG 1 cut(s) 122
BsrI ACTGG 2 cut(s) 105, 206
BssECI CCNNGG 2 cut(s) 69, 254
BssMI GATC 3 cut(s) 40, 204, 382
BssT1I CCWWGG 1 cut(s) 69
Bst2UI CCWGG 2 cut(s) 58, 255
Bst4CI ACNGT 3 cut(s) 25, 187, 447
Bst6I CTCTTC 1 cut(s) 317
BstAPI GCANNNNNTGC 1 cut(s) 220
BstBI TTCGAA 1 cut(s) 320
BstDEI CTNAG 1 cut(s) 93
BstF5I GGATG 3 cut(s) 133, 353, 489
BstKTI GATC 3 cut(s) 43, 207, 385
BstMAI GTCTC 1 cut(s) 14
BstMBI GATC 3 cut(s) 40, 204, 382
BstMWI GCNNNNNNNGC 2 cut(s) 220, 357
BstNI CCWGG 2 cut(s) 58, 255
BstSCI CCNGG 2 cut(s) 56, 253
BstSLI GKGCMC 1 cut(s) 9
BstV1I GCAGC 2 cut(s) 88, 226
Bsu36I CCTNAGG 1 cut(s) 93
BsuRI GGCC 1 cut(s) 245
BtsCI GGATG 3 cut(s) 133, 353, 489
BtsIMutI CAGTG 1 cut(s) 378
Cfr13I GGNCC 1 cut(s) 159
Csp6I GTAC 1 cut(s) 429
CviJI RGCY 4 cut(s) 90, 101, 245, 497
CviKI_1 RGCY 4 cut(s) 90, 101, 245, 497
CviQI GTAC 1 cut(s) 429
DdeI CTNAG 1 cut(s) 93
DpnI GATC 3 cut(s) 42, 206, 384
DpnII GATC 3 cut(s) 40, 204, 382
Eam1104I CTCTTC 1 cut(s) 317
EarI CTCTTC 1 cut(s) 317
Ecl136II GAGCTC 1 cut(s) 497
Eco130I CCWWGG 1 cut(s) 69
Eco24I GRGCYC 1 cut(s) 499
Eco47I GGWCC 1 cut(s) 159
Eco53kI GAGCTC 1 cut(s) 497
Eco81I CCTNAGG 1 cut(s) 93
EcoICRI GAGCTC 1 cut(s) 497
EcoRII CCWGG 2 cut(s) 56, 253
EcoT14I CCWWGG 1 cut(s) 69
EcoT38I GRGCYC 1 cut(s) 499
ErhI CCWWGG 1 cut(s) 69
FaiI YATR 4 cut(s) 78, 226, 271, 333
FbaI TGATCA 1 cut(s) 382
Fnu4HI GCNGC 2 cut(s) 102, 215
FokI GGATG 3 cut(s) 120, 360, 496
FriOI GRGCYC 1 cut(s) 499
Fsp4HI GCNGC 2 cut(s) 102, 215
FspBI CTAG 2 cut(s) 51, 210
GluI GCNGC 2 cut(s) 102, 215
GsuI CTGGAG 1 cut(s) 298
HaeIII GGCC 1 cut(s) 245
HphI GGTGA 1 cut(s) 199
Hpy166II GTNNAC 1 cut(s) 7
Hpy188III TCNNGA 2 cut(s) 51, 143
Hpy8I GTNNAC 1 cut(s) 7
HpyAV CCTTC 1 cut(s) 235
HpyCH4III ACNGT 3 cut(s) 25, 187, 447
HpyCH4IV ACGT 1 cut(s) 413
HpyCH4V TGCA 4 cut(s) 7, 117, 360, 396
HpyF10VI GCNNNNNNNGC 2 cut(s) 220, 357
HpyF3I CTNAG 1 cut(s) 93
HpySE526I ACGT 1 cut(s) 413
Ksp22I TGATCA 1 cut(s) 382
Kzo9I GATC 3 cut(s) 40, 204, 382
Lsp1109I GCAGC 2 cut(s) 88, 226
LweI GCATC 2 cut(s) 210, 338
MaeI CTAG 2 cut(s) 51, 210
MaeII ACGT 1 cut(s) 413
MaeIII GTNAC 1 cut(s) 441
MalI GATC 3 cut(s) 42, 206, 384
MboI GATC 3 cut(s) 40, 204, 382
MboII GAAGA 2 cut(s) 162, 334
MhlI GDGCHC 3 cut(s) 9, 380, 499
MluCI AATT 2 cut(s) 292, 361
MmeI TCCRAC 1 cut(s) 137
MnlI CCTC 6 cut(s) 44, 102, 167, 273, 320, 460
MseI TTAA 2 cut(s) 288, 474
MspR9I CCNGG 2 cut(s) 58, 255
Mva1269I GAATGC 1 cut(s) 117
MvaI CCWGG 2 cut(s) 58, 255
MwoI GCNNNNNNNGC 2 cut(s) 220, 357
NdeII GATC 3 cut(s) 40, 204, 382
NlaIV GGNNCC 1 cut(s) 161
NspV TTCGAA 1 cut(s) 320
PctI GAATGC 1 cut(s) 117
PkrI GCNGC 2 cut(s) 103, 216
Psp124BI GAGCTC 1 cut(s) 499
Psp6I CCWGG 2 cut(s) 56, 253
PspGI CCWGG 2 cut(s) 56, 253
PspN4I GGNNCC 1 cut(s) 161
PspPI GGNCC 1 cut(s) 159
RsaI GTAC 1 cut(s) 430
RsaNI GTAC 1 cut(s) 429
SacI GAGCTC 1 cut(s) 499
SaqAI TTAA 2 cut(s) 288, 474
SatI GCNGC 2 cut(s) 102, 215
Sau3AI GATC 3 cut(s) 40, 204, 382
Sau96I GGNCC 1 cut(s) 159
ScrFI CCNGG 2 cut(s) 58, 255
SduI GDGCHC 3 cut(s) 9, 380, 499
SetI ASST 8 cut(s) 36, 59, 71, 99, 304, 331, 416, 499
SfaNI GCATC 2 cut(s) 210, 338
SfuI TTCGAA 1 cut(s) 320
SinI GGWCC 1 cut(s) 159
SmlI CTYRAG 1 cut(s) 17
SmoI CTYRAG 1 cut(s) 17
Sse9I AATT 2 cut(s) 292, 361
SspMI CTAG 2 cut(s) 51, 210
SstI GAGCTC 1 cut(s) 499
StyD4I CCNGG 2 cut(s) 56, 253
StyI CCWWGG 1 cut(s) 69
TaaI ACNGT 3 cut(s) 25, 187, 447
TaiI ACGT 1 cut(s) 416
TaqI TCGA 1 cut(s) 320
TasI AATT 2 cut(s) 292, 361
Tru1I TTAA 2 cut(s) 288, 474
Tru9I TTAA 2 cut(s) 288, 474
TscAI CASTG 1 cut(s) 385
TseI GCWGC 2 cut(s) 101, 214
TspDTI ATGAA 1 cut(s) 65
TspRI CASTG 1 cut(s) 385
VneI GTGCAC 1 cut(s) 5
VpaK11BI GGWCC 1 cut(s) 159
XbaI TCTAGA 1 cut(s) 50
XspI CTAG 2 cut(s) 51, 210
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.