Rorug04G0254800

Very-long-chain 3-oxoacyl-CoA reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0001251
Physical Location & Seq
Forward (+)
42751063 .. 42753142
2080 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0254800.1

Sequence Viewer

Length: 780 bp
ATGATCCGCATGAGACCAAAAGTGACGGTCCGTGATCCGTTAAACAATTGGAAGGCCAAATGGGTAACGACGGTGGAGAACCCGGAAAAACCAAGACAAATCCTATGGGTAGAATACATGGAAGCGCTGTCGGATGAGGATTTAGATATTACCTCCGTTGATATGATGACCACTGGCCTCCAACGTGGCCATACACCCGAGGCCAAAACGGCGAAACTCATACAGTCTAAGATGCAGGCTCAAGGTAGCAATGCAGAAGAACCAAAGGATAATGCTGCAGACTGTTTGGCTCAAGCCTCGAAACAAAGTGGTAACGTGTATAAGCCATTGACAATGCGTTTTGTCAAATTATTCACTGGTACACCTCCACCTGTTAAGACTATTGATGTTCGTCCAAGCTCACCAGAAAGGAAATTCAGTTGGTCGACGGACTTCCTTGTTGTGCCAAGGAAGTTTTTAGGTATGTCAAGATTCCAATGGGTGAAGCCTGGTGATGTGGATGAGGAATTCTTTGCTTCCCCAGCGGAGATGGTTGGATATATCATGGAGACAAACAAGAAGAGTAATCCAACAGGTAGTATGGCAGAAAAACCAGTGGTTGTTGGAGGTGCTTTTACTTCTCCACGACATCCTCTTGAGTACTGTAGGATTTGTTGCAAGTACATTGACCACTCAGCCTTTTTTTGTCCGTACCTGGAATATGTCCCACGTGGTGTGTTAATGTCTAAAATTTCGGCGGTAGCTGAACCTTTGTTAACGCTAATCCGGTGGGCGGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00062 ko00140 ko01040 ko01100 ko01110 ko01212 map00062 map00140 map01040 map01100 map01110 map01212
Pfam Domains
Protein Families
adh_short

Protein Analysis

259

Amino Acids

29.3

Weight (kDa)

8.71

Isoelectric Point (pI)

36.46

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 425
AciI CCGC 4 cut(s) 7, 524, 737, 773
AclWI GGATC 1 cut(s) 29
AcoI YGGCCR 1 cut(s) 187
AcsI RAATTY 3 cut(s) 413, 506, 729
AcvI CACGTG 1 cut(s) 710
AdeI CACNNNGTG 1 cut(s) 713
AfaI GTAC 4 cut(s) 361, 641, 662, 692
AfeI AGCGCT 1 cut(s) 126
AfiI CCNNNNNNNGG 1 cut(s) 772
AflIII ACRYGT 1 cut(s) 315
AjnI CCWGG 2 cut(s) 487, 693
AluBI AGCT 2 cut(s) 399, 743
AluI AGCT 2 cut(s) 399, 743
Alw26I GTCTC 2 cut(s) 7, 542
AlwI GGATC 1 cut(s) 29
Ama87I CYCGRG 1 cut(s) 197
Aor51HI AGCGCT 1 cut(s) 126
AoxI GGCC 4 cut(s) 54, 175, 187, 201
ApeKI GCWGC 1 cut(s) 275
ApoI RAATTY 3 cut(s) 413, 506, 729
AspLEI GCGC 1 cut(s) 127
AspS9I GGNCC 1 cut(s) 28
AsuC2I CCSGG 1 cut(s) 83
AsuHPI GGTGA 3 cut(s) 393, 493, 503
AvaI CYCGRG 1 cut(s) 197
AvaII GGWCC 1 cut(s) 28
BalI TGGCCA 1 cut(s) 189
BbrPI CACGTG 1 cut(s) 710
BbvI GCAGC 1 cut(s) 262
BccI CCATC 1 cut(s) 523
BceAI ACGGC 1 cut(s) 225
BciT130I CCWGG 2 cut(s) 489, 695
BcnI CCSGG 1 cut(s) 83
BcoDI GTCTC 2 cut(s) 7, 542
BfmI CTRYAG 2 cut(s) 276, 643
BfoI RGCGCY 1 cut(s) 128
BglI GCCNNNNNGGC 1 cut(s) 209
BisI GCNGC 1 cut(s) 276
BlsI GCNGC 1 cut(s) 277
BmcAI AGTACT 1 cut(s) 641
Bme1390I CCNGG 3 cut(s) 83, 489, 695
Bme18I GGWCC 1 cut(s) 28
BmeT110I CYCGRG 1 cut(s) 197
BmgT120I GGNCC 1 cut(s) 28
BmrFI CCNGG 3 cut(s) 83, 489, 695
BmsI GCATC 1 cut(s) 222
BpuEI CTTGAG 3 cut(s) 225, 276, 656
BpuMI CCSGG 1 cut(s) 83
BsaAI YACGTR 1 cut(s) 710
BsaI GGTCTC 1 cut(s) 7
BsaJI CCNNGG 2 cut(s) 198, 446
BsaWI WCCGGW 1 cut(s) 765
Bsc4I CCNNNNNNNGG 1 cut(s) 772
Bse1I ACTGG 3 cut(s) 178, 361, 593
Bse3DI GCAATG 1 cut(s) 256
BseBI CCWGG 2 cut(s) 489, 695
BseDI CCNNGG 2 cut(s) 198, 446
BseGI GGATG 3 cut(s) 139, 505, 628
BseLI CCNNNNNNNGG 1 cut(s) 772
BseMI GCAATG 1 cut(s) 256
BseMII CTCAG 1 cut(s) 687
BseNI ACTGG 3 cut(s) 178, 361, 593
BseXI GCAGC 1 cut(s) 262
BseYI CCCAGC 1 cut(s) 520
BshFI GGCC 4 cut(s) 56, 177, 189, 203
BsiHKCI CYCGRG 1 cut(s) 197
BsiSI CCGG 2 cut(s) 83, 766
BslFI GGGAC 1 cut(s) 689
BslI CCNNNNNNNGG 1 cut(s) 772
BsmAI GTCTC 2 cut(s) 7, 542
BsmFI GGGAC 1 cut(s) 689
BsnI GGCC 4 cut(s) 56, 177, 189, 203
Bso31I GGTCTC 1 cut(s) 7
BsoBI CYCGRG 1 cut(s) 197
Bsp143I GATC 2 cut(s) 3, 34
BspACI CCGC 4 cut(s) 7, 524, 737, 773
BspANI GGCC 4 cut(s) 56, 177, 189, 203
BspCNI CTCAG 1 cut(s) 686
BspMAI CTGCAG 1 cut(s) 280
BspPI GGATC 1 cut(s) 29
BspTNI GGTCTC 1 cut(s) 7
BsrDI GCAATG 1 cut(s) 256
BsrI ACTGG 3 cut(s) 178, 361, 593
BssECI CCNNGG 2 cut(s) 198, 446
BssMI GATC 2 cut(s) 3, 34
BssT1I CCWWGG 1 cut(s) 446
Bst2UI CCWGG 2 cut(s) 489, 695
Bst4CI ACNGT 5 cut(s) 28, 73, 225, 284, 644
Bst6I CTCTTC 1 cut(s) 554
BstBAI YACGTR 1 cut(s) 710
BstC8I GCNNGC 1 cut(s) 237
BstDEI CTNAG 2 cut(s) 228, 673
BstF5I GGATG 3 cut(s) 139, 505, 628
BstH2I RGCGCY 1 cut(s) 128
BstHHI GCGC 1 cut(s) 127
BstKTI GATC 2 cut(s) 6, 37
BstMAI GTCTC 2 cut(s) 7, 542
BstMBI GATC 2 cut(s) 3, 34
BstMWI GCNNNNNNNGC 2 cut(s) 209, 521
BstNI CCWGG 2 cut(s) 489, 695
BstSCI CCNGG 3 cut(s) 81, 487, 693
BstSFI CTRYAG 2 cut(s) 276, 643
BstV1I GCAGC 1 cut(s) 262
BsuRI GGCC 4 cut(s) 56, 177, 189, 203
BtsCI GGATG 3 cut(s) 139, 505, 628
BtsIMutI CAGTG 3 cut(s) 171, 354, 600
Cac8I GCNNGC 1 cut(s) 237
CfoI GCGC 1 cut(s) 127
Cfr13I GGNCC 1 cut(s) 28
CpoI CGGWCCG 1 cut(s) 28
Csp6I GTAC 4 cut(s) 360, 640, 661, 691
CspI CGGWCCG 1 cut(s) 28
CviAII CATG 3 cut(s) 10, 118, 544
CviQI GTAC 4 cut(s) 360, 640, 661, 691
DdeI CTNAG 2 cut(s) 228, 673
DpnI GATC 2 cut(s) 5, 36
DpnII GATC 2 cut(s) 3, 34
DraIII CACNNNGTG 1 cut(s) 713
EaeI YGGCCR 1 cut(s) 187
Eam1104I CTCTTC 1 cut(s) 554
EarI CTCTTC 1 cut(s) 554
Eco130I CCWWGG 1 cut(s) 446
Eco31I GGTCTC 1 cut(s) 7
Eco47I GGWCC 1 cut(s) 28
Eco47III AGCGCT 1 cut(s) 126
Eco72I CACGTG 1 cut(s) 710
Eco88I CYCGRG 1 cut(s) 197
EcoRI GAATTC 1 cut(s) 506
EcoRII CCWGG 2 cut(s) 487, 693
EcoT14I CCWWGG 1 cut(s) 446
ErhI CCWWGG 1 cut(s) 446
FaeI CATG 3 cut(s) 13, 121, 547
FaqI GGGAC 1 cut(s) 689
FatI CATG 3 cut(s) 9, 117, 543
FblI GTMKAC 1 cut(s) 425
Fnu4HI GCNGC 1 cut(s) 276
FokI GGATG 3 cut(s) 146, 512, 615
Fsp4HI GCNGC 1 cut(s) 276
GlaI GCGC 1 cut(s) 126
GluI GCNGC 1 cut(s) 276
GsaI CCCAGC 1 cut(s) 524
HaeII RGCGCY 1 cut(s) 128
HaeIII GGCC 4 cut(s) 56, 177, 189, 203
HapII CCGG 2 cut(s) 83, 766
HhaI GCGC 1 cut(s) 127
Hin1II CATG 3 cut(s) 13, 121, 547
Hin6I GCGC 1 cut(s) 125
HinP1I GCGC 1 cut(s) 125
HincII GTYRAC 2 cut(s) 426, 756
HindII GTYRAC 2 cut(s) 426, 756
HinfI GANTC 1 cut(s) 471
HpaI GTTAAC 1 cut(s) 756
HpaII CCGG 2 cut(s) 83, 766
HphI GGTGA 3 cut(s) 393, 493, 503
Hpy166II GTNNAC 3 cut(s) 362, 426, 756
Hpy188I TCNGA 1 cut(s) 133
Hpy188III TCNNGA 2 cut(s) 468, 635
Hpy8I GTNNAC 3 cut(s) 362, 426, 756
Hpy99I CGWCG 2 cut(s) 73, 430
HpyAV CCTTC 1 cut(s) 46
HpyCH4III ACNGT 5 cut(s) 28, 73, 225, 284, 644
HpyCH4IV ACGT 3 cut(s) 184, 315, 709
HpyCH4V TGCA 4 cut(s) 235, 254, 278, 657
HpyF10VI GCNNNNNNNGC 2 cut(s) 209, 521
HpyF3I CTNAG 2 cut(s) 228, 673
HpySE526I ACGT 3 cut(s) 184, 315, 709
Hsp92II CATG 3 cut(s) 13, 121, 547
HspAI GCGC 1 cut(s) 125
KspAI GTTAAC 1 cut(s) 756
Kzo9I GATC 2 cut(s) 3, 34
Lsp1109I GCAGC 1 cut(s) 262
LweI GCATC 1 cut(s) 222
MaeII ACGT 3 cut(s) 184, 315, 709
MaeIII GTNAC 3 cut(s) 22, 64, 311
MalI GATC 2 cut(s) 5, 36
MboI GATC 2 cut(s) 3, 34
MboII GAAGA 2 cut(s) 269, 571
MfeI CAATTG 1 cut(s) 46
MlsI TGGCCA 1 cut(s) 189
MluCI AATT 5 cut(s) 46, 347, 413, 506, 729
MluNI TGGCCA 1 cut(s) 189
MmeI TCCRAC 5 cut(s) 111, 205, 514, 583, 593
MnlI CCTC 9 cut(s) 130, 163, 188, 193, 307, 375, 496, 599, 642
Mox20I TGGCCA 1 cut(s) 189
MscI TGGCCA 1 cut(s) 189
MseI TTAA 4 cut(s) 41, 375, 719, 755
Msp20I TGGCCA 1 cut(s) 189
MspA1I CMGCKG 1 cut(s) 524
MspI CCGG 2 cut(s) 83, 766
MspR9I CCNGG 3 cut(s) 83, 489, 695
MunI CAATTG 1 cut(s) 46
MvaI CCWGG 2 cut(s) 489, 695
MwoI GCNNNNNNNGC 2 cut(s) 209, 521
NciI CCSGG 1 cut(s) 83
NdeII GATC 2 cut(s) 3, 34
NlaIII CATG 3 cut(s) 13, 121, 547
NmuCI GTSAC 1 cut(s) 22
PfeI GAWTC 1 cut(s) 471
PkrI GCNGC 1 cut(s) 277
PmaCI CACGTG 1 cut(s) 710
PmlI CACGTG 1 cut(s) 710
Ppu21I YACGTR 1 cut(s) 710
Psp6I CCWGG 2 cut(s) 487, 693
PspCI CACGTG 1 cut(s) 710
PspFI CCCAGC 1 cut(s) 520
PspGI CCWGG 2 cut(s) 487, 693
PspPI GGNCC 1 cut(s) 28
PstI CTGCAG 1 cut(s) 280
RsaI GTAC 4 cut(s) 361, 641, 662, 692
RsaNI GTAC 4 cut(s) 360, 640, 661, 691
Rsr2I CGGWCCG 1 cut(s) 28
RsrII CGGWCCG 1 cut(s) 28
SalI GTCGAC 1 cut(s) 424
SaqAI TTAA 4 cut(s) 41, 375, 719, 755
SatI GCNGC 1 cut(s) 276
Sau3AI GATC 2 cut(s) 3, 34
Sau96I GGNCC 1 cut(s) 28
ScaI AGTACT 1 cut(s) 641
ScrFI CCNGG 3 cut(s) 83, 489, 695
SfaNI GCATC 1 cut(s) 222
SfcI CTRYAG 2 cut(s) 276, 643
SinI GGWCC 1 cut(s) 28
SmlI CTYRAG 3 cut(s) 240, 291, 635
SmoI CTYRAG 3 cut(s) 240, 291, 635
Sse9I AATT 5 cut(s) 46, 347, 413, 506, 729
SsiI CCGC 4 cut(s) 7, 524, 737, 773
StyD4I CCNGG 3 cut(s) 81, 487, 693
StyI CCWWGG 1 cut(s) 446
TaaI ACNGT 5 cut(s) 28, 73, 225, 284, 644
TaiI ACGT 3 cut(s) 187, 318, 712
TaqI TCGA 2 cut(s) 299, 425
TasI AATT 5 cut(s) 46, 347, 413, 506, 729
TatI WGTACW 2 cut(s) 639, 660
TfiI GAWTC 1 cut(s) 471
Tru1I TTAA 4 cut(s) 41, 375, 719, 755
Tru9I TTAA 4 cut(s) 41, 375, 719, 755
TscAI CASTG 3 cut(s) 178, 361, 600
TseFI GTSAC 1 cut(s) 22
TseI GCWGC 1 cut(s) 275
Tsp45I GTSAC 1 cut(s) 22
TspGWI ACGGA 5 cut(s) 20, 27, 145, 443, 678
TspRI CASTG 3 cut(s) 178, 361, 600
VpaK11BI GGWCC 1 cut(s) 28
XapI RAATTY 3 cut(s) 413, 506, 729
XmiI GTMKAC 1 cut(s) 425
ZrmI AGTACT 1 cut(s) 641
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.