Belongs to the short-chain dehydrogenases reductases (SDR) family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccII | CGCG | 1 cut(s) | 270 |
| AciI | CCGC | 2 cut(s) | 268, 270 |
| AclWI | GGATC | 1 cut(s) | 142 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 220, 495, 496 |
| AgsI | TTSAA | 2 cut(s) | 191, 201 |
| AluBI | AGCT | 5 cut(s) | 7, 244, 251, 347, 454 |
| AluI | AGCT | 5 cut(s) | 7, 244, 251, 347, 454 |
| AlwI | GGATC | 1 cut(s) | 142 |
| ApeKI | GCWGC | 2 cut(s) | 29, 608 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 235 |
| AspS9I | GGNCC | 1 cut(s) | 577 |
| AsuC2I | CCSGG | 1 cut(s) | 519 |
| AsuHPI | GGTGA | 3 cut(s) | 28, 487, 511 |
| AsuII | TTCGAA | 1 cut(s) | 239 |
| AvaII | GGWCC | 1 cut(s) | 577 |
| BbvI | GCAGC | 2 cut(s) | 41, 595 |
| BccI | CCATC | 3 cut(s) | 78, 158, 451 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 295, 329 |
| BclI | TGATCA | 1 cut(s) | 69 |
| BcnI | CCSGG | 1 cut(s) | 519 |
| BfaI | CTAG | 1 cut(s) | 504 |
| BisI | GCNGC | 2 cut(s) | 30, 609 |
| BlsI | GCNGC | 2 cut(s) | 31, 610 |
| Bme1390I | CCNGG | 1 cut(s) | 519 |
| Bme18I | GGWCC | 1 cut(s) | 577 |
| BmgT120I | GGNCC | 1 cut(s) | 577 |
| BmiI | GGNNCC | 2 cut(s) | 157, 579 |
| BmrFI | CCNGG | 1 cut(s) | 519 |
| BmsI | GCATC | 1 cut(s) | 375 |
| Bpu14I | TTCGAA | 1 cut(s) | 239 |
| BpuEI | CTTGAG | 2 cut(s) | 107, 512 |
| BpuMI | CCSGG | 1 cut(s) | 519 |
| BsaJI | CCNNGG | 3 cut(s) | 113, 268, 573 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 220, 495, 496 |
| Bse1I | ACTGG | 1 cut(s) | 157 |
| BseDI | CCNNGG | 3 cut(s) | 113, 268, 573 |
| BseGI | GGATG | 1 cut(s) | 414 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 220, 495, 496 |
| BseNI | ACTGG | 1 cut(s) | 157 |
| BseRI | GAGGAG | 1 cut(s) | 353 |
| BseXI | GCAGC | 2 cut(s) | 41, 595 |
| Bsh1236I | CGCG | 1 cut(s) | 270 |
| Bsh1285I | CGRYCG | 1 cut(s) | 229 |
| BsiEI | CGRYCG | 1 cut(s) | 229 |
| BsiSI | CCGG | 1 cut(s) | 519 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 220, 495, 496 |
| Bsp119I | TTCGAA | 1 cut(s) | 239 |
| Bsp143I | GATC | 2 cut(s) | 69, 134 |
| BspACI | CCGC | 2 cut(s) | 268, 270 |
| BspFNI | CGCG | 1 cut(s) | 270 |
| BspHI | TCATGA | 2 cut(s) | 283, 436 |
| BspLI | GGNNCC | 2 cut(s) | 157, 579 |
| BspPI | GGATC | 1 cut(s) | 142 |
| BspT104I | TTCGAA | 1 cut(s) | 239 |
| BsrI | ACTGG | 1 cut(s) | 157 |
| BssECI | CCNNGG | 3 cut(s) | 113, 268, 573 |
| BssMI | GATC | 2 cut(s) | 69, 134 |
| BssT1I | CCWWGG | 1 cut(s) | 113 |
| BstBI | TTCGAA | 1 cut(s) | 239 |
| BstDSI | CCRYGG | 1 cut(s) | 268 |
| BstF5I | GGATG | 1 cut(s) | 414 |
| BstFNI | CGCG | 1 cut(s) | 270 |
| BstKTI | GATC | 2 cut(s) | 72, 137 |
| BstMBI | GATC | 2 cut(s) | 69, 134 |
| BstMCI | CGRYCG | 1 cut(s) | 229 |
| BstSCI | CCNGG | 1 cut(s) | 517 |
| BstUI | CGCG | 1 cut(s) | 270 |
| BstV1I | GCAGC | 2 cut(s) | 41, 595 |
| BstX2I | RGATCY | 1 cut(s) | 134 |
| BstYI | RGATCY | 1 cut(s) | 134 |
| BtgI | CCRYGG | 1 cut(s) | 268 |
| BtsCI | GGATG | 1 cut(s) | 414 |
| BtsI | GCAGTG | 1 cut(s) | 516 |
| BtsIMutI | CAGTG | 3 cut(s) | 150, 159, 516 |
| CciI | TCATGA | 2 cut(s) | 283, 436 |
| Cfr13I | GGNCC | 1 cut(s) | 577 |
| Cfr42I | CCGCGG | 1 cut(s) | 271 |
| CviAII | CATG | 4 cut(s) | 97, 284, 437, 481 |
| DpnI | GATC | 2 cut(s) | 71, 136 |
| DpnII | GATC | 2 cut(s) | 69, 134 |
| Eco130I | CCWWGG | 1 cut(s) | 113 |
| Eco47I | GGWCC | 1 cut(s) | 577 |
| EcoO109I | RGGNCCY | 1 cut(s) | 577 |
| EcoT14I | CCWWGG | 1 cut(s) | 113 |
| ErhI | CCWWGG | 1 cut(s) | 113 |
| FaeI | CATG | 4 cut(s) | 100, 287, 440, 484 |
| FatI | CATG | 4 cut(s) | 96, 283, 436, 480 |
| FauI | CCCGC | 1 cut(s) | 263 |
| FbaI | TGATCA | 1 cut(s) | 69 |
| Fnu4HI | GCNGC | 2 cut(s) | 30, 609 |
| FokI | GGATG | 1 cut(s) | 421 |
| Fsp4HI | GCNGC | 2 cut(s) | 30, 609 |
| FspBI | CTAG | 1 cut(s) | 504 |
| GluI | GCNGC | 2 cut(s) | 30, 609 |
| HapII | CCGG | 1 cut(s) | 519 |
| Hin1II | CATG | 4 cut(s) | 100, 287, 440, 484 |
| HinfI | GANTC | 2 cut(s) | 118, 264 |
| HpaII | CCGG | 1 cut(s) | 519 |
| HphI | GGTGA | 3 cut(s) | 28, 487, 511 |
| Hpy166II | GTNNAC | 1 cut(s) | 478 |
| Hpy188I | TCNGA | 2 cut(s) | 565, 574 |
| Hpy188III | TCNNGA | 4 cut(s) | 284, 437, 529, 621 |
| Hpy8I | GTNNAC | 1 cut(s) | 478 |
| HpyAV | CCTTC | 3 cut(s) | 246, 356, 591 |
| HpyCH4V | TGCA | 2 cut(s) | 404, 425 |
| Hsp92II | CATG | 4 cut(s) | 100, 287, 440, 484 |
| Ksp22I | TGATCA | 1 cut(s) | 69 |
| KspI | CCGCGG | 1 cut(s) | 271 |
| Kzo9I | GATC | 2 cut(s) | 69, 134 |
| LmnI | GCTCC | 5 cut(s) | 4, 161, 249, 451, 542 |
| LpnPI | CCDG | 3 cut(s) | 138, 390, 532 |
| Lsp1109I | GCAGC | 2 cut(s) | 41, 595 |
| LweI | GCATC | 1 cut(s) | 375 |
| MaeI | CTAG | 1 cut(s) | 504 |
| MaeIII | GTNAC | 1 cut(s) | 499 |
| MalI | GATC | 2 cut(s) | 71, 136 |
| MboI | GATC | 2 cut(s) | 69, 134 |
| MboII | GAAGA | 2 cut(s) | 544, 547 |
| MflI | RGATCY | 1 cut(s) | 134 |
| MluCI | AATT | 4 cut(s) | 73, 144, 326, 546 |
| MnlI | CCTC | 7 cut(s) | 120, 265, 331, 352, 433, 568, 600 |
| MroXI | GAANNNNTTC | 1 cut(s) | 235 |
| MseI | TTAA | 4 cut(s) | 104, 210, 300, 396 |
| MspA1I | CMGCKG | 1 cut(s) | 270 |
| MspI | CCGG | 1 cut(s) | 519 |
| MspR9I | CCNGG | 1 cut(s) | 519 |
| MvnI | CGCG | 1 cut(s) | 270 |
| NciI | CCSGG | 1 cut(s) | 519 |
| NdeII | GATC | 2 cut(s) | 69, 134 |
| NlaIII | CATG | 4 cut(s) | 100, 287, 440, 484 |
| NlaIV | GGNNCC | 2 cut(s) | 157, 579 |
| NmuCI | GTSAC | 1 cut(s) | 499 |
| NspV | TTCGAA | 1 cut(s) | 239 |
| PagI | TCATGA | 2 cut(s) | 283, 436 |
| PdmI | GAANNNNTTC | 1 cut(s) | 235 |
| PfeI | GAWTC | 2 cut(s) | 118, 264 |
| PkrI | GCNGC | 2 cut(s) | 31, 610 |
| PpuMI | RGGWCCY | 1 cut(s) | 577 |
| Psp5II | RGGWCCY | 1 cut(s) | 577 |
| PspN4I | GGNNCC | 2 cut(s) | 157, 579 |
| PspPI | GGNCC | 1 cut(s) | 577 |
| PspPPI | RGGWCCY | 1 cut(s) | 577 |
| PsuI | RGATCY | 1 cut(s) | 134 |
| SacII | CCGCGG | 1 cut(s) | 271 |
| SaqAI | TTAA | 4 cut(s) | 104, 210, 300, 396 |
| SatI | GCNGC | 2 cut(s) | 30, 609 |
| Sau3AI | GATC | 2 cut(s) | 69, 134 |
| Sau96I | GGNCC | 1 cut(s) | 577 |
| ScrFI | CCNGG | 1 cut(s) | 519 |
| SfaNI | GCATC | 1 cut(s) | 375 |
| Sfr303I | CCGCGG | 1 cut(s) | 271 |
| SfuI | TTCGAA | 1 cut(s) | 239 |
| SgrBI | CCGCGG | 1 cut(s) | 271 |
| SinI | GGWCC | 1 cut(s) | 577 |
| SmlI | CTYRAG | 2 cut(s) | 122, 527 |
| SmoI | CTYRAG | 2 cut(s) | 122, 527 |
| Sse9I | AATT | 4 cut(s) | 73, 144, 326, 546 |
| SsiI | CCGC | 2 cut(s) | 268, 270 |
| SspMI | CTAG | 1 cut(s) | 504 |
| StyD4I | CCNGG | 1 cut(s) | 517 |
| StyI | CCWWGG | 1 cut(s) | 113 |
| TaqI | TCGA | 3 cut(s) | 229, 239, 315 |
| TaqII | GACCGA | 1 cut(s) | 215 |
| TasI | AATT | 4 cut(s) | 73, 144, 326, 546 |
| TfiI | GAWTC | 2 cut(s) | 118, 264 |
| Tru1I | TTAA | 4 cut(s) | 104, 210, 300, 396 |
| Tru9I | TTAA | 4 cut(s) | 104, 210, 300, 396 |
| TscAI | CASTG | 3 cut(s) | 157, 166, 516 |
| TseFI | GTSAC | 1 cut(s) | 499 |
| TseI | GCWGC | 2 cut(s) | 29, 608 |
| Tsp45I | GTSAC | 1 cut(s) | 499 |
| TspDTI | ATGAA | 4 cut(s) | 10, 272, 300, 425 |
| TspRI | CASTG | 3 cut(s) | 157, 166, 516 |
| VpaK11BI | GGWCC | 1 cut(s) | 577 |
| XmnI | GAANNNNTTC | 1 cut(s) | 235 |
| XspI | CTAG | 1 cut(s) | 504 |