pycom06g00270

Encoded by

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0000423
Physical Location & Seq
Reverse (-)
246336 .. 249306
2971 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1245 bp
ATGGAACTTCTGCAGCTGTTCGTGACTGCCTCAATTCCGGTGTTGAAAGTGCTTTTGATAACAGCACTTGGCTCTTATCTTGCTCTTGATCGTGTCGATATTTTGGGGGAAGTTACTAGAAAGAACTTAAACACTGTTGTATTTTATGTGTTCAGTCCTGCTCTGGTGGGTTCCAACCTTGCGAAGACAATTACATATGAAAGCATGGTTAAAATGTGGTTCATGCCAGTGAATATCCTCATCACGTTTATAATTGGTTCCGTGCTCGGATGGATTCTCATCCAATTAACAAGGCCTCCTGCCCATCTGCGAGGCCTTGTCTTGGGTTGCTGTGCTGCAGGAAATTTGGGTAACTTGCTCCTCATTATAGTCCCAGCAGTCTGTAAAGAAAAAGGGAGCCCGTTTGGAGCTCCTGATGTCTGTCACACATATGCTATGGCTTATGCTTCGCTCTCAATGGCGATAGGAGCCGTTTATTTGTGGTCCTATGTATATAATATTGTGCGGATATCTTCAACAAGGGGCACCCAAGATTCTAATCAGTCCACGGAAAGGTCCCCTACATCCGACCAAGTAAGTTGCACTGAGCCGCTGCTTTCTTCAAAGGAATATGTCATAGTTGAGGAAAATGGGGATCAATATTCACTGCCATACACTGCTGTGCCTGAAGAAAAACCAAAGGTGACAACTTCAGGTAAACTAAAGCAACGTATAGTGATGGTGTTTGAAAAGATCAATCTGAAAACAATATTTGCCCCTTCAACTATTGGAGCGATTGTTGGTTTTGCAATCGGAGTCATTCCTCAGATTCGAAAATTACTGATAGGAGATGGTGCTCCTCTACGGGTGATTCAAGATACTGCTTATTTGTTGGGAGACGGAGCCATCCCAGCTCTCACTTTGATAATAGGAGGAAACCTGCTTAAGGGTTTGAGAGGGTCAGGGATTCAGAAATCTCTTGTTGTTGGCATCATAGTTGTTCGTTATGTTGCCCTGCCTCTTATAGGCATATTGGTTATTAAGGGGGCACTGAAATTTGGGTTGGTGCACTCTGATCCATTATATCTGTTTGTTCTTCTGCTTCAGTTTTCACTCCCACCTGCGATGAACATAGGAACAATGACGCAATTATTCGGAGCAGGAGAGAATGAATGTTCAGTTATCATGCTGTGGACTTATGCTTTCGCGTCACTGTCACTTACATTCTGGTCCGCCGCCTTCATGTGGATTGTGACGCGACTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005215 GO:0005575 GO:0006810 GO:0008150 GO:0016020 GO:0016021 GO:0022857 GO:0031224 GO:0044425 GO:0051179 GO:0051234 GO:0055085
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Mem_trans

Protein Analysis

415

Amino Acids

44.88

Weight (kDa)

9.0

Isoelectric Point (pI)

35.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mem_trans PF03547 10 - 403 4.1e-78 Membrane transport protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000423)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G76520 AT1G76520 AT1G76530 AT1G76530 AT1G76530
fragaria_vesca FvH4_5g26311 FvH4_5g26311 FvH4_5g26311 FvH4_5g26311 FvH4_5g26311 FvH4_5g26311 FvH4_5g26330 FvH4_5g26330 FvH4_5g26330 FvH4_5g26330 FvH4_5g26330 FvH4_5g26330 FvH4_5g26330 FvH4_5g26330 FvH4_5g26330 FvH4_5g26330 FvH4_5g26330 FvH4_5g26370 FvH4_5g26370
malus_domestica MD05G1225800.v1.1 MD06G1002600.v1.1 MD06G1002700.v1.1
prunus_persica Prupe.5G004000_v2.0.a1 Prupe.5G004000_v2.0.a1 Prupe.5G004000_v2.0.a1 Prupe.5G004000_v2.0.a1 Prupe.5G004100_v2.0.a1 Prupe.5G004100_v2.0.a1 Prupe.5G004100_v2.0.a1 Prupe.5G004100_v2.0.a1 Prupe.5G004100_v2.0.a1 Prupe.5G004100_v2.0.a1 Prupe.5G004100_v2.0.a1 Prupe.5G004100_v2.0.a1 Prupe.5G004300_v2.0.a1
pyrus_communis pycom05g20790 pycom06g00270 pycom06g00280
rosa_chinensis RchiOBHm_Chr7g0217021 RchiOBHm_Chr7g0217081 RchiOBHm_Chr7g0217111 RchiOBHm_Chr7g0217141 RchiOBHm_Chr7g0217181 RchiOBHm_Chr7g0219341
rosa_laevigata RLG00000002504 RLG00000002506 RLG00000002510 RLG00000002512
rosa_multiflora Rmu_sc0001725.1_g000022 Rmu_sc0002460.1_g000002 Rmu_sc0003132.1_g000001 Rmu_sc0007359.1_g000005 Rmu_sc0007359.1_g000009 Rmu_sc0018492.1_g000001 Rmu_sc0039353.1_g000001
rosa_roxburghii Rroxscaffold_3G00242520 Rroxscaffold_3G00242540 Rroxscaffold_3G00242560 Rroxscaffold_3G00242600
rosa_rugosa Rorug07G0165700 Rorug07G0165700 Rorug07G0165800 Rorug07G0165800 Rorug07G0166400 Rorug07G0166500 Rorug07G0166600 Rorug07G0166700 Rorug07G0166800 Rorug07G0166900 Rorug07G0167000 Rorug07G0167000 Rorug07G0167100
rosa_samantha Rh7AG305200 Rh7AG305300 Rh7AG305700 Rh7AG305900 Rh7AG327400 Rh7CG324200 Rh7CG324400 Rh7CG324900 Rh7CG344700 Rh7DG305400 Rh7DG305500 Rh7DG305800 Rh7DG323000
rosa_wichuraiana Rw0G004530 Rw3G011620 Rw7G025930 Rw7G025960 Rw7G025970 Rw7G025990 Rw7G027580

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 251
AarI CACCTGC 1 cut(s) 1108
Acc36I ACCTGC 2 cut(s) 927, 1108
AccB1I GGYRCC 1 cut(s) 524
AccII CGCG 2 cut(s) 1187, 1237
AciI CCGC 4 cut(s) 505, 590, 1212, 1215
AclWI GGATC 2 cut(s) 642, 1049
AcsI RAATTY 2 cut(s) 343, 1034
AcuI CTGAAG 3 cut(s) 675, 687, 1067
AfiI CCNNNNNNNGG 5 cut(s) 322, 552, 925, 1004, 1224
AflII CTTAAG 1 cut(s) 923
AgsI TTSAA 6 cut(s) 46, 516, 603, 728, 762, 854
AjuI GAANNNNNNNTTGG 2 cut(s) 1025, 1057
AleI CACNNNNGTG 1 cut(s) 659
AluBI AGCT 3 cut(s) 16, 410, 893
AluI AGCT 3 cut(s) 16, 410, 893
Alw21I GWGCWC 4 cut(s) 267, 412, 838, 1050
Alw26I GTCTC 1 cut(s) 870
Alw44I GTGCAC 1 cut(s) 1046
AlwI GGATC 2 cut(s) 642, 1049
AoxI GGCC 2 cut(s) 293, 313
ApaLI GTGCAC 1 cut(s) 1046
ApeKI GCWGC 3 cut(s) 13, 335, 592
ApoI RAATTY 2 cut(s) 343, 1034
AspS9I GGNCC 3 cut(s) 483, 555, 1209
AsuHPI GGTGA 2 cut(s) 694, 859
AsuII TTCGAA 1 cut(s) 811
AvaII GGWCC 3 cut(s) 483, 555, 1209
BaeGI GKGCMC 3 cut(s) 527, 1030, 1050
BanI GGYRCC 1 cut(s) 524
BanII GRGCYC 2 cut(s) 401, 412
BbsI GAAGAC 1 cut(s) 191
Bbv12I GWGCWC 4 cut(s) 267, 412, 838, 1050
BbvI GCAGC 3 cut(s) 25, 322, 579
BccI CCATC 5 cut(s) 264, 312, 712, 824, 893
BceAI ACGGC 1 cut(s) 455
BcoDI GTCTC 1 cut(s) 870
BfaI CTAG 1 cut(s) 117
BfmI CTRYAG 2 cut(s) 11, 336
BfrI CTTAAG 1 cut(s) 923
BfuAI ACCTGC 2 cut(s) 927, 1108
BisI GCNGC 5 cut(s) 14, 336, 590, 593, 1215
BlsI GCNGC 5 cut(s) 15, 337, 591, 594, 1216
Bme18I GGWCC 3 cut(s) 483, 555, 1209
BmgT120I GGNCC 3 cut(s) 483, 555, 1209
BmiI GGNNCC 7 cut(s) 172, 259, 398, 469, 526, 557, 883
BmsI GCATC 1 cut(s) 978
BpiI GAAGAC 1 cut(s) 191
Bpu14I TTCGAA 1 cut(s) 811
BsaBI GATNNNNATC 2 cut(s) 278, 537
BsaJI CCNNGG 1 cut(s) 546
BsaWI WCCGGW 1 cut(s) 37
BsaXI ACNNNNNCTCC 4 cut(s) 280, 310, 762, 792
Bsc4I CCNNNNNNNGG 5 cut(s) 322, 552, 925, 1004, 1224
Bse1I ACTGG 1 cut(s) 227
Bse8I GATNNNNATC 2 cut(s) 278, 537
BseDI CCNNGG 1 cut(s) 546
BseGI GGATG 4 cut(s) 275, 279, 563, 885
BseJI GATNNNNATC 2 cut(s) 278, 537
BseLI CCNNNNNNNGG 5 cut(s) 322, 552, 925, 1004, 1224
BseMII CTCAG 2 cut(s) 576, 818
BseNI ACTGG 1 cut(s) 227
BseRI GAGGAG 2 cut(s) 350, 828
BseSI GKGCMC 3 cut(s) 527, 1030, 1050
BseXI GCAGC 3 cut(s) 25, 322, 579
BseYI CCCAGC 2 cut(s) 373, 889
Bsh1236I CGCG 2 cut(s) 1187, 1237
BshFI GGCC 2 cut(s) 295, 315
BshNI GGYRCC 1 cut(s) 524
BsiHKAI GWGCWC 4 cut(s) 267, 412, 838, 1050
BsiSI CCGG 1 cut(s) 38
BslFI GGGAC 2 cut(s) 356, 541
BslI CCNNNNNNNGG 5 cut(s) 322, 552, 925, 1004, 1224
BsmAI GTCTC 1 cut(s) 870
BsmBI CGTCTC 1 cut(s) 870
BsmFI GGGAC 2 cut(s) 356, 541
BsnI GGCC 2 cut(s) 295, 315
Bsp119I TTCGAA 1 cut(s) 811
Bsp1286I GDGCHC 7 cut(s) 267, 401, 412, 527, 838, 1030, 1050
Bsp143I GATC 4 cut(s) 88, 634, 732, 1054
BspACI CCGC 4 cut(s) 505, 590, 1212, 1215
BspANI GGCC 2 cut(s) 295, 315
BspCNI CTCAG 2 cut(s) 577, 817
BspFNI CGCG 2 cut(s) 1187, 1237
BspLI GGNNCC 7 cut(s) 172, 259, 398, 469, 526, 557, 883
BspMAI CTGCAG 2 cut(s) 15, 340
BspMI ACCTGC 2 cut(s) 927, 1108
BspPI GGATC 2 cut(s) 642, 1049
BspT104I TTCGAA 1 cut(s) 811
BspT107I GGYRCC 1 cut(s) 524
BspTI CTTAAG 1 cut(s) 923
BsrI ACTGG 1 cut(s) 227
BssECI CCNNGG 1 cut(s) 546
BssMI GATC 4 cut(s) 88, 634, 732, 1054
Bst4CI ACNGT 3 cut(s) 136, 1194, 1242
BstAFI CTTAAG 1 cut(s) 923
BstBI TTCGAA 1 cut(s) 811
BstDEI CTNAG 2 cut(s) 585, 804
BstDSI CCRYGG 1 cut(s) 546
BstENI CCTNNNNNAGG 2 cut(s) 923, 1002
BstF5I GGATG 4 cut(s) 275, 279, 563, 885
BstFNI CGCG 2 cut(s) 1187, 1237
BstKTI GATC 4 cut(s) 91, 637, 735, 1057
BstMAI GTCTC 1 cut(s) 870
BstMBI GATC 4 cut(s) 88, 634, 732, 1054
BstMWI GCNNNNNNNGC 2 cut(s) 467, 890
BstSFI CTRYAG 2 cut(s) 11, 336
BstSLI GKGCMC 3 cut(s) 527, 1030, 1050
BstUI CGCG 2 cut(s) 1187, 1237
BstV1I GCAGC 3 cut(s) 25, 322, 579
BstV2I GAAGAC 1 cut(s) 191
BsuRI GGCC 2 cut(s) 295, 315
BtgI CCRYGG 1 cut(s) 546
BtgZI GCGATG 1 cut(s) 1118
BtsCI GGATG 4 cut(s) 275, 279, 563, 885
BtsI GCAGTG 2 cut(s) 644, 654
BtsIMutI CAGTG 7 cut(s) 132, 234, 582, 644, 654, 1028, 1190
BveI ACCTGC 2 cut(s) 927, 1108
Cfr13I GGNCC 3 cut(s) 483, 555, 1209
CseI GACGC 2 cut(s) 1132, 1176
CviAII CATG 4 cut(s) 205, 223, 1165, 1222
DdeI CTNAG 2 cut(s) 585, 804
DpnI GATC 4 cut(s) 90, 636, 734, 1056
DpnII GATC 4 cut(s) 88, 634, 732, 1054
EciI GGCGGA 1 cut(s) 1201
Ecl136II GAGCTC 1 cut(s) 410
Eco147I AGGCCT 2 cut(s) 295, 315
Eco24I GRGCYC 2 cut(s) 401, 412
Eco32I GATATC 1 cut(s) 510
Eco47I GGWCC 3 cut(s) 483, 555, 1209
Eco53kI GAGCTC 1 cut(s) 410
Eco57I CTGAAG 3 cut(s) 675, 687, 1067
EcoICRI GAGCTC 1 cut(s) 410
EcoNI CCTNNNNNAGG 2 cut(s) 923, 1002
EcoO109I RGGNCCY 1 cut(s) 555
EcoRV GATATC 1 cut(s) 510
EcoT38I GRGCYC 2 cut(s) 401, 412
Esp3I CGTCTC 1 cut(s) 870
FaeI CATG 4 cut(s) 208, 226, 1168, 1225
FaqI GGGAC 2 cut(s) 356, 541
FatI CATG 4 cut(s) 204, 222, 1164, 1221
FauNDI CATATG 2 cut(s) 196, 430
Fnu4HI GCNGC 5 cut(s) 14, 336, 590, 593, 1215
FokI GGATG 4 cut(s) 266, 282, 550, 872
FriOI GRGCYC 2 cut(s) 401, 412
Fsp4HI GCNGC 5 cut(s) 14, 336, 590, 593, 1215
FspBI CTAG 1 cut(s) 117
GluI GCNGC 5 cut(s) 14, 336, 590, 593, 1215
GsaI CCCAGC 2 cut(s) 377, 893
HaeIII GGCC 2 cut(s) 295, 315
HapII CCGG 1 cut(s) 38
HgaI GACGC 2 cut(s) 1132, 1176
Hin1II CATG 4 cut(s) 208, 226, 1168, 1225
HinfI GANTC 6 cut(s) 274, 533, 795, 808, 850, 946
HpaII CCGG 1 cut(s) 38
HphI GGTGA 2 cut(s) 694, 859
Hpy166II GTNNAC 4 cut(s) 546, 698, 1048, 1173
Hpy188I TCNGA 8 cut(s) 269, 568, 741, 794, 807, 951, 1054, 1136
Hpy188III TCNNGA 4 cut(s) 22, 86, 413, 854
Hpy8I GTNNAC 4 cut(s) 546, 698, 1048, 1173
HpyAV CCTTC 2 cut(s) 768, 1228
HpyCH4III ACNGT 3 cut(s) 136, 1194, 1242
HpyCH4IV ACGT 2 cut(s) 245, 709
HpyCH4V TGCA 5 cut(s) 13, 338, 582, 788, 1048
HpyF10VI GCNNNNNNNGC 2 cut(s) 467, 890
HpyF3I CTNAG 2 cut(s) 585, 804
HpySE526I ACGT 2 cut(s) 245, 709
Hsp92II CATG 4 cut(s) 208, 226, 1168, 1225
Kzo9I GATC 4 cut(s) 88, 634, 732, 1054
LmnI GCTCC 9 cut(s) 363, 396, 407, 415, 467, 770, 841, 881, 1136
Lsp1109I GCAGC 3 cut(s) 25, 322, 579
LweI GCATC 1 cut(s) 978
MaeI CTAG 1 cut(s) 117
MaeII ACGT 2 cut(s) 245, 709
MaeIII GTNAC 8 cut(s) 22, 112, 350, 422, 682, 1188, 1194, 1231
MalI GATC 4 cut(s) 90, 636, 734, 1056
MboI GATC 4 cut(s) 88, 634, 732, 1054
MboII GAAGA 5 cut(s) 196, 504, 591, 680, 1067
MhlI GDGCHC 7 cut(s) 267, 401, 412, 527, 838, 1030, 1050
MluCI AATT 8 cut(s) 33, 189, 252, 284, 343, 815, 1034, 1127
MlyI GAGTC 1 cut(s) 804
MmeI TCCRAC 2 cut(s) 198, 591
MseI TTAA 5 cut(s) 128, 210, 287, 924, 1020
MslI CAYNNNNRTG 3 cut(s) 227, 429, 659
MspA1I CMGCKG 2 cut(s) 16, 592
MspCI CTTAAG 1 cut(s) 923
MspI CCGG 1 cut(s) 38
MvnI CGCG 2 cut(s) 1187, 1237
MwoI GCNNNNNNNGC 2 cut(s) 467, 890
NdeI CATATG 2 cut(s) 196, 430
NdeII GATC 4 cut(s) 88, 634, 732, 1054
NlaIII CATG 4 cut(s) 208, 226, 1168, 1225
NlaIV GGNNCC 7 cut(s) 172, 259, 398, 469, 526, 557, 883
NmuCI GTSAC 6 cut(s) 22, 422, 682, 1188, 1194, 1231
NspV TTCGAA 1 cut(s) 811
OliI CACNNNNGTG 1 cut(s) 659
PaqCI CACCTGC 1 cut(s) 1108
PceI AGGCCT 2 cut(s) 295, 315
PfeI GAWTC 5 cut(s) 274, 533, 808, 850, 946
PkrI GCNGC 5 cut(s) 15, 337, 591, 594, 1216
PleI GAGTC 1 cut(s) 803
PpsI GAGTC 1 cut(s) 803
PpuMI RGGWCCY 1 cut(s) 555
PsiI TTATAA 1 cut(s) 251
Psp124BI GAGCTC 1 cut(s) 412
Psp5II RGGWCCY 1 cut(s) 555
PspFI CCCAGC 2 cut(s) 373, 889
PspN4I GGNNCC 7 cut(s) 172, 259, 398, 469, 526, 557, 883
PspPI GGNCC 3 cut(s) 483, 555, 1209
PspPPI RGGWCCY 1 cut(s) 555
PstI CTGCAG 2 cut(s) 15, 340
PvuII CAGCTG 1 cut(s) 16
RseI CAYNNNNRTG 3 cut(s) 227, 429, 659
SacI GAGCTC 1 cut(s) 412
SaqAI TTAA 5 cut(s) 128, 210, 287, 924, 1020
SatI GCNGC 5 cut(s) 14, 336, 590, 593, 1215
Sau3AI GATC 4 cut(s) 88, 634, 732, 1054
Sau96I GGNCC 3 cut(s) 483, 555, 1209
SchI GAGTC 1 cut(s) 804
SduI GDGCHC 7 cut(s) 267, 401, 412, 527, 838, 1030, 1050
SfaNI GCATC 1 cut(s) 978
SfcI CTRYAG 2 cut(s) 11, 336
SfuI TTCGAA 1 cut(s) 811
SinI GGWCC 3 cut(s) 483, 555, 1209
SmiMI CAYNNNNRTG 3 cut(s) 227, 429, 659
SmlI CTYRAG 1 cut(s) 923
SmoI CTYRAG 1 cut(s) 923
Sse9I AATT 8 cut(s) 33, 189, 252, 284, 343, 815, 1034, 1127
SseBI AGGCCT 2 cut(s) 295, 315
SsiI CCGC 4 cut(s) 505, 590, 1212, 1215
SspI AATATT 3 cut(s) 499, 641, 750
SspMI CTAG 1 cut(s) 117
SstI GAGCTC 1 cut(s) 412
StuI AGGCCT 2 cut(s) 295, 315
TaaI ACNGT 3 cut(s) 136, 1194, 1242
TaiI ACGT 2 cut(s) 248, 712
TaqI TCGA 2 cut(s) 96, 811
TasI AATT 8 cut(s) 33, 189, 252, 284, 343, 815, 1034, 1127
TauI GCSGC 2 cut(s) 592, 1217
TfiI GAWTC 5 cut(s) 274, 533, 808, 850, 946
Tru1I TTAA 5 cut(s) 128, 210, 287, 924, 1020
Tru9I TTAA 5 cut(s) 128, 210, 287, 924, 1020
TscAI CASTG 7 cut(s) 139, 234, 589, 651, 661, 1035, 1197
TseFI GTSAC 6 cut(s) 22, 422, 682, 1188, 1194, 1231
TseI GCWGC 3 cut(s) 13, 335, 592
Tsp45I GTSAC 6 cut(s) 22, 422, 682, 1188, 1194, 1231
TspDTI ATGAA 5 cut(s) 211, 213, 1121, 1164, 1210
TspGWI ACGGA 3 cut(s) 250, 563, 894
TspRI CASTG 7 cut(s) 139, 234, 589, 651, 661, 1035, 1197
Vha464I CTTAAG 1 cut(s) 923
VneI GTGCAC 1 cut(s) 1046
VpaK11BI GGWCC 3 cut(s) 483, 555, 1209
XagI CCTNNNNNAGG 2 cut(s) 923, 1002
XapI RAATTY 2 cut(s) 343, 1034
XspI CTAG 1 cut(s) 117
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.