pycom12g23290

Encoded by

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0001068
Physical Location & Seq
Forward (+)
23936288 .. 23939213
2926 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1209 bp
ATGGCTTACGTTCCACCACACAGGCGGCACTCGAAGGAACCGGAGATGCCATTGCTCACTCCAGAGCTGCTTGCTCCTCAGTTCAAGAAAAACTTAAATGTCAAGCCATATTATAAGTCTAAAGTGGATGAAAATATAGTTTATGCACACCATTCTACACATGGATGGTGCGCCATCCCTTTGGATGACGAGAACCAGTTCCCATCTTCTGTTAATCTTGAGCCCGTTACCTTGGAGTCTGATGAGCTGAATGTTGGAGGAAATCATCTAGCTTTGATCAATACTGGTCTAGATAATGGCGGTGAGGTGAAATGGAACTTGCCAAGGAGCCCTTGGGAATCTATAACAGAAAATGTGCTGGAAGACTTACTTTTGGCATTTAAACACGCGAGGAGTGAAATGAAGTCTGCAAAGCCCAAAGAAGCATATCCAACTTTGATTGCCAGAGTGGGGAAAGTACTCTTTCGCAGGAGCCCTTCGGTTAACATGGAATCCATTAGAAAAAATTTGTGTGCTGAAATCCCGAGACAATGGAGGCAATTATTTTACACAAACACTCCTGTTTCATATAAGGAAAAGATTGTGAACGAAGTTGTCCCGAAAATTGGAGTTGATTTCGAAGAGGAGGAAGATATATACGATGTACAGTTGTCCGATTCTACGAGTCCAGATTCAACTCTCTTCTGCAGATGTCGGGTAATGAAAGAACATGGAAAGCTACAACTCTGTGAGATCAAGTTGAAACCAGTGCGTAACATGGTAAAGGACATTTCATGCACTACTAAGAATCTGGACCTGAGACTAATTCTGTGCACGAAGAAACTCGTAACTGATCTGACTAATGATGAGATGCAAAGCATCACGGATCTGATTAATTCTGCGATTCTAGATCCAGACTTGAAGGGTGGATTGAGTTGGCCCCCGGGGAAGGAGTCTTCTGGAGATAAATACAGAGTTGTTAGGTTTACGCACGTAATAGTTAATACGTATAGAAACTCATCACTGAGACTGAAAGTAAAACACTATGATCGATTTGATTTGAGAACCTTAACTGGCGAAGCTTCTTGGGGGACAAGTCTGATGCTGGAAAACGTCGTGTCAAAGTTACAGGAAGAGAATGTCGAAGCCAGCACAATTTCTGAGATACTCAAGGAAGATATGCAGTTGATATGGGATAACTTCTTGAGCTGTGAACGTTTTCTATCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

403

Amino Acids

46.03

Weight (kDa)

6.12

Isoelectric Point (pI)

43.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7903 PF25475 43 - 394 6.7e-141 Domain of unknown function (DUF7903)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 114
AccII CGCG 1 cut(s) 389
AciI CCGC 2 cut(s) 25, 300
AclI AACGTT 1 cut(s) 1195
AclWI GGATC 2 cut(s) 873, 884
AcsI RAATTY 1 cut(s) 505
AfaI GTAC 2 cut(s) 459, 645
AfiI CCNNNNNNNGG 3 cut(s) 450, 605, 928
AgsI TTSAA 4 cut(s) 85, 675, 742, 901
AluBI AGCT 6 cut(s) 67, 247, 272, 718, 1061, 1188
AluI AGCT 6 cut(s) 67, 247, 272, 718, 1061, 1188
Alw21I GWGCWC 1 cut(s) 815
Alw26I GTCTC 3 cut(s) 520, 793, 1000
Alw44I GTGCAC 1 cut(s) 811
AlwI GGATC 2 cut(s) 873, 884
Ama87I CYCGRG 2 cut(s) 523, 922
AoxI GGCC 1 cut(s) 917
ApaLI GTGCAC 1 cut(s) 811
ApeKI GCWGC 1 cut(s) 67
ApoI RAATTY 1 cut(s) 505
AseI ATTAAT 1 cut(s) 873
Asp700I GAANNNNTTC 2 cut(s) 197, 1197
AspLEI GCGC 1 cut(s) 173
AspS9I GGNCC 2 cut(s) 793, 918
AsuC2I CCSGG 2 cut(s) 923, 924
AsuHPI GGTGA 2 cut(s) 314, 319
AsuII TTCGAA 1 cut(s) 618
AvaI CYCGRG 2 cut(s) 523, 922
AvaII GGWCC 1 cut(s) 793
BaeGI GKGCMC 1 cut(s) 815
BanII GRGCYC 3 cut(s) 225, 332, 476
BbsI GAAGAC 2 cut(s) 369, 927
Bbv12I GWGCWC 1 cut(s) 815
BbvI GCAGC 1 cut(s) 54
BccI CCATC 3 cut(s) 159, 182, 211
BclI TGATCA 1 cut(s) 276
BcnI CCSGG 2 cut(s) 923, 924
BcoDI GTCTC 3 cut(s) 520, 793, 1000
BfaI CTAG 3 cut(s) 269, 290, 887
BfmI CTRYAG 1 cut(s) 685
BisI GCNGC 2 cut(s) 26, 68
BlsI GCNGC 2 cut(s) 27, 69
BmcAI AGTACT 1 cut(s) 459
Bme1390I CCNGG 2 cut(s) 923, 924
Bme18I GGWCC 1 cut(s) 793
BmeT110I CYCGRG 2 cut(s) 523, 922
BmgT120I GGNCC 2 cut(s) 793, 918
BmiI GGNNCC 4 cut(s) 39, 329, 473, 920
BmrFI CCNGG 2 cut(s) 923, 924
BmsI GCATC 4 cut(s) 36, 840, 867, 1071
BpiI GAAGAC 2 cut(s) 369, 927
BpmI CTGGAG 2 cut(s) 45, 960
Bpu14I TTCGAA 1 cut(s) 618
BpuEI CTTGAG 3 cut(s) 239, 1133, 1204
BpuMI CCSGG 2 cut(s) 923, 924
Bsa29I ATCGAT 1 cut(s) 1030
BsaAI YACGTR 2 cut(s) 973, 987
BsaJI CCNNGG 6 cut(s) 231, 323, 332, 921, 922, 923
BsaWI WCCGGW 1 cut(s) 40
BsaXI ACNNNNNCTCC 2 cut(s) 541, 571
Bsc4I CCNNNNNNNGG 3 cut(s) 450, 605, 928
Bse1I ACTGG 4 cut(s) 196, 289, 746, 1057
Bse3DI GCAATG 1 cut(s) 50
BseCI ATCGAT 1 cut(s) 1030
BseDI CCNNGG 6 cut(s) 231, 323, 332, 921, 922, 923
BseGI GGATG 4 cut(s) 133, 170, 174, 190
BseLI CCNNNNNNNGG 3 cut(s) 450, 605, 928
BseMI GCAATG 1 cut(s) 50
BseMII CTCAG 4 cut(s) 92, 788, 995, 1131
BseNI ACTGG 4 cut(s) 196, 289, 746, 1057
BseRI GAGGAG 3 cut(s) 66, 406, 638
BseSI GKGCMC 1 cut(s) 815
BseXI GCAGC 1 cut(s) 54
Bsh1236I CGCG 1 cut(s) 389
BshFI GGCC 1 cut(s) 919
BshVI ATCGAT 1 cut(s) 1030
BsiHKAI GWGCWC 1 cut(s) 815
BsiHKCI CYCGRG 2 cut(s) 523, 922
BsiSI CCGG 2 cut(s) 41, 923
BslFI GGGAC 2 cut(s) 581, 1084
BslI CCNNNNNNNGG 3 cut(s) 450, 605, 928
BsmAI GTCTC 3 cut(s) 520, 793, 1000
BsmFI GGGAC 2 cut(s) 581, 1084
BsnI GGCC 1 cut(s) 919
BsoBI CYCGRG 2 cut(s) 523, 922
Bsp119I TTCGAA 1 cut(s) 618
Bsp1286I GDGCHC 4 cut(s) 225, 332, 476, 815
Bsp1407I TGTACA 1 cut(s) 643
Bsp143I GATC 6 cut(s) 276, 732, 832, 865, 889, 1027
BspACI CCGC 2 cut(s) 25, 300
BspANI GGCC 1 cut(s) 919
BspCNI CTCAG 4 cut(s) 91, 789, 996, 1132
BspDI ATCGAT 1 cut(s) 1030
BspFNI CGCG 1 cut(s) 389
BspHI TCATGA 1 cut(s) 1205
BspLI GGNNCC 4 cut(s) 39, 329, 473, 920
BspMAI CTGCAG 1 cut(s) 689
BspPI GGATC 2 cut(s) 873, 884
BspT104I TTCGAA 1 cut(s) 618
BsrDI GCAATG 1 cut(s) 50
BsrGI TGTACA 1 cut(s) 643
BsrI ACTGG 4 cut(s) 196, 289, 746, 1057
BssECI CCNNGG 6 cut(s) 231, 323, 332, 921, 922, 923
BssMI GATC 6 cut(s) 276, 732, 832, 865, 889, 1027
BssT1I CCWWGG 3 cut(s) 231, 323, 332
Bst4CI ACNGT 1 cut(s) 648
Bst6I CTCTTC 3 cut(s) 615, 686, 1107
BstAUI TGTACA 1 cut(s) 643
BstBAI YACGTR 2 cut(s) 973, 987
BstBI TTCGAA 1 cut(s) 618
BstC8I GCNNGC 2 cut(s) 72, 1129
BstDEI CTNAG 5 cut(s) 78, 783, 797, 1004, 1140
BstF5I GGATG 4 cut(s) 133, 170, 174, 190
BstFNI CGCG 1 cut(s) 389
BstHHI GCGC 1 cut(s) 173
BstKTI GATC 6 cut(s) 279, 735, 835, 868, 892, 1030
BstMAI GTCTC 3 cut(s) 520, 793, 1000
BstMBI GATC 6 cut(s) 276, 732, 832, 865, 889, 1027
BstSCI CCNGG 2 cut(s) 921, 922
BstSFI CTRYAG 1 cut(s) 685
BstSLI GKGCMC 1 cut(s) 815
BstSNI TACGTA 1 cut(s) 987
BstUI CGCG 1 cut(s) 389
BstV1I GCAGC 1 cut(s) 54
BstV2I GAAGAC 2 cut(s) 369, 927
BstX2I RGATCY 2 cut(s) 865, 889
BstXI CCANNNNNNTGG 1 cut(s) 181
BstYI RGATCY 2 cut(s) 865, 889
Bsu15I ATCGAT 1 cut(s) 1030
BsuRI GGCC 1 cut(s) 919
BsuTUI ATCGAT 1 cut(s) 1030
BtsCI GGATG 4 cut(s) 133, 170, 174, 190
BtsIMutI CAGTG 2 cut(s) 753, 1001
Cac8I GCNNGC 2 cut(s) 72, 1129
CciI TCATGA 1 cut(s) 1205
CfoI GCGC 1 cut(s) 173
Cfr13I GGNCC 2 cut(s) 793, 918
Cfr9I CCCGGG 1 cut(s) 922
ClaI ATCGAT 1 cut(s) 1030
Csp6I GTAC 2 cut(s) 458, 644
CviAII CATG 6 cut(s) 161, 487, 710, 757, 774, 1206
CviQI GTAC 2 cut(s) 458, 644
DdeI CTNAG 5 cut(s) 78, 783, 797, 1004, 1140
DpnI GATC 6 cut(s) 278, 734, 834, 867, 891, 1029
DpnII GATC 6 cut(s) 276, 732, 832, 865, 889, 1027
DraI TTTAAA 1 cut(s) 382
Eam1104I CTCTTC 3 cut(s) 615, 686, 1107
EarI CTCTTC 3 cut(s) 615, 686, 1107
Eco105I TACGTA 1 cut(s) 987
Eco130I CCWWGG 3 cut(s) 231, 323, 332
Eco24I GRGCYC 3 cut(s) 225, 332, 476
Eco47I GGWCC 1 cut(s) 793
Eco88I CYCGRG 2 cut(s) 523, 922
EcoT14I CCWWGG 3 cut(s) 231, 323, 332
EcoT38I GRGCYC 3 cut(s) 225, 332, 476
ErhI CCWWGG 3 cut(s) 231, 323, 332
FaeI CATG 6 cut(s) 164, 490, 713, 760, 777, 1209
FalI AAGNNNNNCTT 6 cut(s) 77, 109, 316, 348, 354, 386
FaqI GGGAC 2 cut(s) 581, 1084
FatI CATG 6 cut(s) 160, 486, 709, 756, 773, 1205
FbaI TGATCA 1 cut(s) 276
Fnu4HI GCNGC 2 cut(s) 26, 68
FokI GGATG 4 cut(s) 140, 161, 177, 197
FriOI GRGCYC 3 cut(s) 225, 332, 476
Fsp4HI GCNGC 2 cut(s) 26, 68
FspBI CTAG 3 cut(s) 269, 290, 887
GlaI GCGC 1 cut(s) 172
GluI GCNGC 2 cut(s) 26, 68
GsuI CTGGAG 2 cut(s) 45, 960
HaeIII GGCC 1 cut(s) 919
HapII CCGG 2 cut(s) 41, 923
HhaI GCGC 1 cut(s) 173
Hin1II CATG 6 cut(s) 164, 490, 713, 760, 777, 1209
Hin6I GCGC 1 cut(s) 171
HinP1I GCGC 1 cut(s) 171
HincII GTYRAC 1 cut(s) 484
HindII GTYRAC 1 cut(s) 484
HindIII AAGCTT 1 cut(s) 1059
HinfI GANTC 9 cut(s) 236, 338, 491, 656, 664, 671, 787, 883, 932
HpaI GTTAAC 1 cut(s) 484
HpaII CCGG 2 cut(s) 41, 923
HphI GGTGA 2 cut(s) 314, 319
Hpy166II GTNNAC 5 cut(s) 484, 586, 813, 966, 1193
Hpy188I TCNGA 6 cut(s) 241, 655, 837, 870, 1080, 1141
Hpy8I GTNNAC 5 cut(s) 484, 586, 813, 966, 1193
Hpy99I CGWCG 1 cut(s) 1097
HpyAV CCTTC 4 cut(s) 28, 486, 895, 922
HpyCH4III ACNGT 1 cut(s) 648
HpyCH4IV ACGT 5 cut(s) 9, 972, 986, 1092, 1195
HpyCH4V TGCA 7 cut(s) 146, 410, 687, 777, 813, 853, 1162
HpyF3I CTNAG 5 cut(s) 78, 783, 797, 1004, 1140
HpySE526I ACGT 5 cut(s) 9, 972, 986, 1092, 1195
Hsp92II CATG 6 cut(s) 164, 490, 713, 760, 777, 1209
HspAI GCGC 1 cut(s) 171
Ksp22I TGATCA 1 cut(s) 276
KspAI GTTAAC 1 cut(s) 484
Kzo9I GATC 6 cut(s) 276, 732, 832, 865, 889, 1027
LmnI GCTCC 3 cut(s) 79, 327, 471
Lsp1109I GCAGC 1 cut(s) 54
LweI GCATC 4 cut(s) 36, 840, 867, 1071
MaeI CTAG 3 cut(s) 269, 290, 887
MaeII ACGT 5 cut(s) 9, 972, 986, 1092, 1195
MaeIII GTNAC 4 cut(s) 226, 752, 826, 1104
MalI GATC 6 cut(s) 278, 734, 834, 867, 891, 1029
MboI GATC 6 cut(s) 276, 732, 832, 865, 889, 1027
MboII GAAGA 9 cut(s) 198, 374, 632, 641, 673, 829, 927, 1124, 1166
MflI RGATCY 2 cut(s) 865, 889
MhlI GDGCHC 4 cut(s) 225, 332, 476, 815
MluCI AATT 6 cut(s) 505, 539, 603, 804, 874, 1134
MlyI GAGTC 3 cut(s) 245, 673, 941
MmeI TCCRAC 2 cut(s) 235, 455
MnlI CCTC 7 cut(s) 87, 251, 298, 384, 528, 616, 619
MroXI GAANNNNTTC 2 cut(s) 197, 1197
MseI TTAA 7 cut(s) 95, 213, 381, 483, 873, 981, 1049
MslI CAYNNNNRTG 1 cut(s) 163
MspI CCGG 2 cut(s) 41, 923
MspR9I CCNGG 2 cut(s) 923, 924
MvnI CGCG 1 cut(s) 389
NciI CCSGG 2 cut(s) 923, 924
NdeII GATC 6 cut(s) 276, 732, 832, 865, 889, 1027
NlaIII CATG 6 cut(s) 164, 490, 713, 760, 777, 1209
NlaIV GGNNCC 4 cut(s) 39, 329, 473, 920
NspV TTCGAA 1 cut(s) 618
PagI TCATGA 1 cut(s) 1205
PdmI GAANNNNTTC 2 cut(s) 197, 1197
PfeI GAWTC 6 cut(s) 338, 491, 656, 671, 787, 883
PkrI GCNGC 2 cut(s) 27, 69
PleI GAGTC 3 cut(s) 244, 672, 940
PpsI GAGTC 3 cut(s) 244, 672, 940
Ppu21I YACGTR 2 cut(s) 973, 987
PshBI ATTAAT 1 cut(s) 873
PsiI TTATAA 1 cut(s) 114
Psp1406I AACGTT 1 cut(s) 1195
PspN4I GGNNCC 4 cut(s) 39, 329, 473, 920
PspPI GGNCC 2 cut(s) 793, 918
PstI CTGCAG 1 cut(s) 689
PsuI RGATCY 2 cut(s) 865, 889
RsaI GTAC 2 cut(s) 459, 645
RsaNI GTAC 2 cut(s) 458, 644
RseI CAYNNNNRTG 1 cut(s) 163
SaqAI TTAA 7 cut(s) 95, 213, 381, 483, 873, 981, 1049
SatI GCNGC 2 cut(s) 26, 68
Sau3AI GATC 6 cut(s) 276, 732, 832, 865, 889, 1027
Sau96I GGNCC 2 cut(s) 793, 918
ScaI AGTACT 1 cut(s) 459
SchI GAGTC 3 cut(s) 245, 673, 941
ScrFI CCNGG 2 cut(s) 923, 924
SduI GDGCHC 4 cut(s) 225, 332, 476, 815
SfaNI GCATC 4 cut(s) 36, 840, 867, 1071
SfcI CTRYAG 1 cut(s) 685
SfuI TTCGAA 1 cut(s) 618
SinI GGWCC 1 cut(s) 793
SmaI CCCGGG 1 cut(s) 924
SmiMI CAYNNNNRTG 1 cut(s) 163
SmlI CTYRAG 3 cut(s) 218, 1148, 1183
SmoI CTYRAG 3 cut(s) 218, 1148, 1183
SnaBI TACGTA 1 cut(s) 987
Sse9I AATT 6 cut(s) 505, 539, 603, 804, 874, 1134
SsiI CCGC 2 cut(s) 25, 300
SspMI CTAG 3 cut(s) 269, 290, 887
StyD4I CCNGG 2 cut(s) 921, 922
StyI CCWWGG 3 cut(s) 231, 323, 332
TaaI ACNGT 1 cut(s) 648
TaiI ACGT 5 cut(s) 12, 975, 989, 1095, 1198
TaqI TCGA 4 cut(s) 32, 618, 1030, 1122
TasI AATT 6 cut(s) 505, 539, 603, 804, 874, 1134
TatI WGTACW 2 cut(s) 457, 643
TauI GCSGC 1 cut(s) 28
TfiI GAWTC 6 cut(s) 338, 491, 656, 671, 787, 883
Tru1I TTAA 7 cut(s) 95, 213, 381, 483, 873, 981, 1049
Tru9I TTAA 7 cut(s) 95, 213, 381, 483, 873, 981, 1049
TscAI CASTG 2 cut(s) 753, 1008
TseI GCWGC 1 cut(s) 67
TspDTI ATGAA 5 cut(s) 144, 416, 555, 716, 762
TspGWI ACGGA 1 cut(s) 878
TspMI CCCGGG 1 cut(s) 922
TspRI CASTG 2 cut(s) 753, 1008
VneI GTGCAC 1 cut(s) 811
VpaK11BI GGWCC 1 cut(s) 793
VspI ATTAAT 1 cut(s) 873
XapI RAATTY 1 cut(s) 505
XbaI TCTAGA 2 cut(s) 289, 886
XcmI CCANNNNNNNNNTGG 2 cut(s) 158, 330
XmaI CCCGGG 1 cut(s) 922
XmnI GAANNNNTTC 2 cut(s) 197, 1197
XspI CTAG 3 cut(s) 269, 290, 887
ZrmI AGTACT 1 cut(s) 459
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.