RchiOBHm_Chr0c39g0503211

Methionine adenosyltransferase 2 subunit

Design Primers CRISPR Design

Basic Information

Type: Sequence Only
Biological Identity
rosa_chinensis
Unknown
OG0001020
Physical Location & Seq
Reverse (-)
0 .. 0
1 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 657 bp
ATGGATTTGAAGCCATTTCTCATCAGTTTGGTCCGGTCACCTGATGTGGTAGTAAACTGTGCTGCACTATCGGTTCATCGTGCCTGTGAAATGGATCCTGCAGCAGCTATGTCAGTTAATGTGCCATCTTCTCTTGTTAATTGGTTATCAAGCTTAGAAGAGAGTAATTATCTACTGATCCATTTGTCAACTGATCAAGTTTATGAAGGGGTGAAGTCTTTTTACAAGGAAGATGATGAAGTTTTCATTACTGAGAAATGCTCGAACTTTGCAATTTTGAGAAGCAGTATCATCTTTGGGCCACAGACAATCTCACCGGTTTCAAAATCTCTTCCGATTCAGTGGGTTGATGGTGTCCTCTCCAAAGGAAATACAACCGAATTCTTTCATGATGAGTTTCGATGCCCTGTGTATGTAAAGGATGTCATAGCAATCATACTTGCTTTGTCCAAGACATGGATATCAGAGGCTAAGCAAAGAAATTTGCTACTGAATGTTGGTGGACTGGACAGGGTATCCCGTGTTCAAATGGCTGAGACAGTTGCTGATATAAGGGGATACAACCTCTCATTAATTAAATCTGTATCTGCATCATCGACGCTTGGTATTTCTCCTATTTCATTTCGAGATGGTGTCAGATTGACGCTTGAACTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RmlD_sub_bind

Protein Analysis

218

Amino Acids

24.09

Weight (kDa)

5.42

Isoelectric Point (pI)

40.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RmlD_sub_bind PF04321 13 - 80 1.1e-09 RmlD substrate binding domain
RmlD_sub_bind PF04321 77 - 198 2.8e-06 RmlD substrate binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 456
AclWI GGATC 3 cut(s) 89, 102, 172
AcsI RAATTY 2 cut(s) 380, 481
AfiI CCNNNNNNNGG 1 cut(s) 456
AgeI ACCGGT 1 cut(s) 316
AgsI TTSAA 4 cut(s) 10, 324, 527, 650
AluBI AGCT 2 cut(s) 107, 153
AluI AGCT 2 cut(s) 107, 153
Alw26I GTCTC 1 cut(s) 530
AlwI GGATC 3 cut(s) 89, 102, 172
AlwNI CAGNNNCTG 1 cut(s) 545
AoxI GGCC 1 cut(s) 299
ApeKI GCWGC 3 cut(s) 62, 101, 104
ApoI RAATTY 2 cut(s) 380, 481
AseI ATTAAT 1 cut(s) 572
AsiGI ACCGGT 1 cut(s) 316
Asp700I GAANNNNTTC 1 cut(s) 384
AspS9I GGNCC 2 cut(s) 31, 299
AsuHPI GGTGA 3 cut(s) 30, 223, 306
AvaII GGWCC 1 cut(s) 31
BamHI GGATCC 1 cut(s) 94
BarI GAAGNNNNNNTAC 2 cut(s) 206, 238
BbvI GCAGC 3 cut(s) 49, 113, 116
BccI CCATC 3 cut(s) 133, 344, 623
BciVI GTATCC 2 cut(s) 526, 551
BclI TGATCA 1 cut(s) 193
BcoDI GTCTC 1 cut(s) 530
BfmI CTRYAG 1 cut(s) 99
BfuI GTATCC 2 cut(s) 526, 551
BisI GCNGC 3 cut(s) 63, 102, 105
BlpI GCTNAGC 1 cut(s) 471
BlsI GCNGC 3 cut(s) 64, 103, 106
Bme18I GGWCC 1 cut(s) 31
BmgT120I GGNCC 2 cut(s) 31, 299
BmiI GGNNCC 1 cut(s) 96
BmsI GCATC 2 cut(s) 392, 599
BplI GAGNNNNNCTC 2 cut(s) 245, 277
Bpu1102I GCTNAGC 1 cut(s) 471
BsaWI WCCGGW 2 cut(s) 33, 316
Bsc4I CCNNNNNNNGG 1 cut(s) 456
Bse118I RCCGGY 1 cut(s) 316
Bse1I ACTGG 1 cut(s) 510
BseGI GGATG 1 cut(s) 427
BseLI CCNNNNNNNGG 1 cut(s) 456
BseMII CTCAG 2 cut(s) 243, 525
BseNI ACTGG 1 cut(s) 510
BseXI GCAGC 3 cut(s) 49, 113, 116
BsgI GTGCAG 1 cut(s) 48
BshFI GGCC 1 cut(s) 301
BshTI ACCGGT 1 cut(s) 316
BsiSI CCGG 2 cut(s) 34, 317
BslI CCNNNNNNNGG 1 cut(s) 456
BsmAI GTCTC 1 cut(s) 530
BsnI GGCC 1 cut(s) 301
Bsp143I GATC 3 cut(s) 94, 177, 193
Bsp1720I GCTNAGC 1 cut(s) 471
BspANI GGCC 1 cut(s) 301
BspCNI CTCAG 2 cut(s) 244, 526
BspHI TCATGA 1 cut(s) 388
BspLI GGNNCC 1 cut(s) 96
BspMAI CTGCAG 1 cut(s) 103
BspPI GGATC 3 cut(s) 89, 102, 172
BsrFI RCCGGY 1 cut(s) 316
BsrI ACTGG 1 cut(s) 510
BssAI RCCGGY 1 cut(s) 316
BssMI GATC 3 cut(s) 94, 177, 193
Bst4CI ACNGT 3 cut(s) 59, 541, 654
Bst6I CTCTTC 2 cut(s) 153, 336
BstDEI CTNAG 4 cut(s) 154, 252, 471, 534
BstEII GGTNACC 1 cut(s) 36
BstF5I GGATG 1 cut(s) 427
BstKTI GATC 3 cut(s) 97, 180, 196
BstMAI GTCTC 1 cut(s) 530
BstMBI GATC 3 cut(s) 94, 177, 193
BstPI GGTNACC 1 cut(s) 36
BstSFI CTRYAG 1 cut(s) 99
BstV1I GCAGC 3 cut(s) 49, 113, 116
BstX2I RGATCY 1 cut(s) 94
BstYI RGATCY 1 cut(s) 94
BsuI GTATCC 2 cut(s) 526, 551
BsuRI GGCC 1 cut(s) 301
BtsCI GGATG 1 cut(s) 427
BtsIMutI CAGTG 1 cut(s) 347
CaiI CAGNNNCTG 1 cut(s) 545
CciI TCATGA 1 cut(s) 388
Cfr10I RCCGGY 1 cut(s) 316
Cfr13I GGNCC 2 cut(s) 31, 299
CseI GACGC 2 cut(s) 607, 652
CspAI ACCGGT 1 cut(s) 316
CviAII CATG 2 cut(s) 389, 456
CviJI RGCY 6 cut(s) 13, 107, 153, 301, 470, 533
CviKI_1 RGCY 6 cut(s) 13, 107, 153, 301, 470, 533
DdeI CTNAG 4 cut(s) 154, 252, 471, 534
DpnI GATC 3 cut(s) 96, 179, 195
DpnII GATC 3 cut(s) 94, 177, 193
Eam1104I CTCTTC 2 cut(s) 153, 336
EarI CTCTTC 2 cut(s) 153, 336
Eco32I GATATC 1 cut(s) 462
Eco47I GGWCC 1 cut(s) 31
Eco91I GGTNACC 1 cut(s) 36
EcoO65I GGTNACC 1 cut(s) 36
EcoRI GAATTC 1 cut(s) 380
EcoRV GATATC 1 cut(s) 462
FaeI CATG 2 cut(s) 392, 459
FaiI YATR 8 cut(s) 110, 204, 390, 414, 428, 437, 457, 551
FatI CATG 2 cut(s) 388, 455
FbaI TGATCA 1 cut(s) 193
Fnu4HI GCNGC 3 cut(s) 63, 102, 105
FokI GGATG 1 cut(s) 434
Fsp4HI GCNGC 3 cut(s) 63, 102, 105
GluI GCNGC 3 cut(s) 63, 102, 105
HaeIII GGCC 1 cut(s) 301
HapII CCGG 2 cut(s) 34, 317
HgaI GACGC 2 cut(s) 607, 652
Hin1II CATG 2 cut(s) 392, 459
HincII GTYRAC 1 cut(s) 189
HindII GTYRAC 1 cut(s) 189
HindIII AAGCTT 1 cut(s) 151
HinfI GANTC 1 cut(s) 337
HpaII CCGG 2 cut(s) 34, 317
HphI GGTGA 3 cut(s) 30, 223, 306
Hpy166II GTNNAC 3 cut(s) 55, 189, 503
Hpy188I TCNGA 3 cut(s) 336, 466, 638
Hpy188III TCNNGA 2 cut(s) 389, 626
Hpy8I GTNNAC 3 cut(s) 55, 189, 503
Hpy99I CGWCG 1 cut(s) 601
HpyAV CCTTC 1 cut(s) 200
HpyCH4III ACNGT 3 cut(s) 59, 541, 654
HpyCH4V TGCA 4 cut(s) 65, 101, 272, 590
HpyF3I CTNAG 4 cut(s) 154, 252, 471, 534
Hsp92II CATG 2 cut(s) 392, 459
Ksp22I TGATCA 1 cut(s) 193
Kzo9I GATC 3 cut(s) 94, 177, 193
LpnPI CCDG 8 cut(s) 47, 54, 97, 111, 330, 420, 491, 496
Lsp1109I GCAGC 3 cut(s) 49, 113, 116
LweI GCATC 2 cut(s) 392, 599
MaeIII GTNAC 1 cut(s) 36
MalI GATC 3 cut(s) 96, 179, 195
MboI GATC 3 cut(s) 94, 177, 193
MboII GAAGA 4 cut(s) 120, 170, 242, 323
MflI RGATCY 1 cut(s) 94
MluCI AATT 6 cut(s) 139, 166, 273, 380, 481, 573
MnlI CCTC 3 cut(s) 368, 460, 575
MroXI GAANNNNTTC 1 cut(s) 384
MseI TTAA 4 cut(s) 117, 138, 572, 576
MspI CCGG 2 cut(s) 34, 317
NdeII GATC 3 cut(s) 94, 177, 193
NlaIII CATG 2 cut(s) 392, 459
NlaIV GGNNCC 1 cut(s) 96
NmuCI GTSAC 1 cut(s) 36
PacI TTAATTAA 1 cut(s) 576
PagI TCATGA 1 cut(s) 388
PdmI GAANNNNTTC 1 cut(s) 384
PfeI GAWTC 1 cut(s) 337
PflMI CCANNNNNTGG 1 cut(s) 456
PinAI ACCGGT 1 cut(s) 316
PkrI GCNGC 3 cut(s) 64, 103, 106
PshBI ATTAAT 1 cut(s) 572
PspEI GGTNACC 1 cut(s) 36
PspN4I GGNNCC 1 cut(s) 96
PspPI GGNCC 2 cut(s) 31, 299
PsrI GAACNNNNNNTAC 2 cut(s) 507, 539
PstI CTGCAG 1 cut(s) 103
PstNI CAGNNNCTG 1 cut(s) 545
PsuI RGATCY 1 cut(s) 94
SaqAI TTAA 4 cut(s) 117, 138, 572, 576
SatI GCNGC 3 cut(s) 63, 102, 105
Sau3AI GATC 3 cut(s) 94, 177, 193
Sau96I GGNCC 2 cut(s) 31, 299
SetI ASST 4 cut(s) 43, 109, 155, 567
SfaNI GCATC 2 cut(s) 392, 599
SfcI CTRYAG 1 cut(s) 99
SinI GGWCC 1 cut(s) 31
Sse9I AATT 6 cut(s) 139, 166, 273, 380, 481, 573
TaaI ACNGT 3 cut(s) 59, 541, 654
TaqI TCGA 4 cut(s) 263, 400, 596, 625
TasI AATT 6 cut(s) 139, 166, 273, 380, 481, 573
TfiI GAWTC 1 cut(s) 337
Tru1I TTAA 4 cut(s) 117, 138, 572, 576
Tru9I TTAA 4 cut(s) 117, 138, 572, 576
TscAI CASTG 1 cut(s) 347
TseFI GTSAC 1 cut(s) 36
TseI GCWGC 3 cut(s) 62, 101, 104
Tsp45I GTSAC 1 cut(s) 36
TspDTI ATGAA 6 cut(s) 65, 219, 235, 252, 377, 609
TspRI CASTG 1 cut(s) 347
Van91I CCANNNNNTGG 1 cut(s) 456
VpaK11BI GGWCC 1 cut(s) 31
VspI ATTAAT 1 cut(s) 572
XapI RAATTY 2 cut(s) 380, 481
XmnI GAANNNNTTC 1 cut(s) 384
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.