RchiOBHm_Chr0c39g0503281

Methionine adenosyltransferase 2 subunit

Design Primers CRISPR Design

Basic Information

Type: Sequence Only
Biological Identity
rosa_chinensis
Unknown
OG0001020
Physical Location & Seq
Reverse (-)
0 .. 0
1 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 693 bp
ATGGAATTGAAGTCATTTCTCATCTGTTTGGTCCGGTCACCTGATGTGGTAGTAAACTGTGCTGCACTTTCGGTTCCTCGTGCCTGTGAAATGGATCCTGCTGCAGCTATGTCAGTTAATGTGCCATCTTCTCTTGTTAATTGGTTATCGAGCTTAGAAGAGACTCAAGAGAGTAATTACCTTCTGATCCATTTGTCAACTGATCAAGGGGTGAAGTCCTTTTACAAGGAAGATGGTGAAGTTGTTCCAGTAAATGTTTATGGGAAATCAAAAGTGGCAGCTGAGCAGTTCATTTCCGAGAAATGCTCAAACTTTGCAATTTTGAGAAGCAGTATCATCTTTGGGCCACAAACAATCTCACCGTGGGTTGATGGTGTCCTCTCCAAAGGAAATACAACGGAATTCTTTCATGATGAGTTTCGATGCCCTGTGTATGTAAAGGATGTCGTAGCAATCATACTTGCTTTGTCCAAGACATGGATATCAGGGTATCCCGTGTACAAATGGCTGGAGACGGTTGCTGATATAAGGGGACACAACCTCTCATTAATTAAATCTGTATCTGCATCATCGGTTGATCGTGGAGTTATGTCTCCTGCTGACATATCCATGGATATAACTAAGCTAGTTCAGACGCTTGGTATTTCTCCTATTTCATTTCGAGATGGTGTCAGATTGACGCTTGAACTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RmlD_sub_bind

Protein Analysis

230

Amino Acids

25.25

Weight (kDa)

5.25

Isoelectric Point (pI)

41.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RmlD_sub_bind PF04321 13 - 228 1.3e-24 RmlD substrate binding domain
Epimerase PF01370 13 - 156 2e-07 NAD dependent epimerase/dehydratase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 477
AclWI GGATC 3 cut(s) 89, 102, 181
AcsI RAATTY 1 cut(s) 401
AfaI GTAC 1 cut(s) 500
AfiI CCNNNNNNNGG 1 cut(s) 477
AgsI TTSAA 2 cut(s) 10, 686
AluBI AGCT 4 cut(s) 107, 153, 281, 625
AluI AGCT 4 cut(s) 107, 153, 281, 625
Alw26I GTCTC 3 cut(s) 155, 506, 597
AlwI GGATC 3 cut(s) 89, 102, 181
AoxI GGCC 1 cut(s) 344
ApeKI GCWGC 4 cut(s) 62, 101, 104, 278
ApoI RAATTY 1 cut(s) 401
AseI ATTAAT 1 cut(s) 548
Asp700I GAANNNNTTC 2 cut(s) 243, 405
AspS9I GGNCC 2 cut(s) 31, 344
AsuHPI GGTGA 4 cut(s) 30, 223, 248, 351
AvaII GGWCC 1 cut(s) 31
BamHI GGATCC 1 cut(s) 94
BarI GAAGNNNNNNTAC 2 cut(s) 206, 238
BauI CACGAG 1 cut(s) 78
BbvI GCAGC 4 cut(s) 49, 88, 116, 290
BccI CCATC 4 cut(s) 133, 227, 365, 659
BciVI GTATCC 1 cut(s) 501
BclI TGATCA 1 cut(s) 202
BcoDI GTCTC 3 cut(s) 155, 506, 597
BfaI CTAG 1 cut(s) 626
BfmI CTRYAG 1 cut(s) 102
BfuI GTATCC 1 cut(s) 501
BisI GCNGC 4 cut(s) 63, 102, 105, 279
BlpI GCTNAGC 1 cut(s) 282
BlsI GCNGC 4 cut(s) 64, 103, 106, 280
Bme18I GGWCC 1 cut(s) 31
BmgT120I GGNCC 2 cut(s) 31, 344
BmiI GGNNCC 2 cut(s) 75, 96
BmsI GCATC 2 cut(s) 413, 575
BplI GAGNNNNNCTC 2 cut(s) 290, 322
BpmI CTGGAG 1 cut(s) 530
Bpu1102I GCTNAGC 1 cut(s) 282
BpuEI CTTGAG 1 cut(s) 150
BsaJI CCNNGG 2 cut(s) 362, 609
BsaWI WCCGGW 1 cut(s) 33
Bsc4I CCNNNNNNNGG 1 cut(s) 477
Bse1I ACTGG 1 cut(s) 248
BseDI CCNNGG 2 cut(s) 362, 609
BseGI GGATG 1 cut(s) 448
BseLI CCNNNNNNNGG 1 cut(s) 477
BseMII CTCAG 1 cut(s) 273
BseNI ACTGG 1 cut(s) 248
BseXI GCAGC 4 cut(s) 49, 88, 116, 290
BsgI GTGCAG 1 cut(s) 48
BshFI GGCC 1 cut(s) 346
BsiSI CCGG 1 cut(s) 34
BslFI GGGAC 1 cut(s) 546
BslI CCNNNNNNNGG 1 cut(s) 477
BsmAI GTCTC 3 cut(s) 155, 506, 597
BsmBI CGTCTC 1 cut(s) 506
BsmFI GGGAC 1 cut(s) 546
BsnI GGCC 1 cut(s) 346
Bsp1407I TGTACA 1 cut(s) 498
Bsp143I GATC 4 cut(s) 94, 186, 202, 577
Bsp1720I GCTNAGC 1 cut(s) 282
Bsp19I CCATGG 1 cut(s) 609
BspANI GGCC 1 cut(s) 346
BspCNI CTCAG 1 cut(s) 274
BspHI TCATGA 1 cut(s) 409
BspLI GGNNCC 2 cut(s) 75, 96
BspMAI CTGCAG 1 cut(s) 106
BspPI GGATC 3 cut(s) 89, 102, 181
BsrGI TGTACA 1 cut(s) 498
BsrI ACTGG 1 cut(s) 248
BssECI CCNNGG 2 cut(s) 362, 609
BssMI GATC 4 cut(s) 94, 186, 202, 577
BssSI CACGAG 1 cut(s) 78
BssT1I CCWWGG 1 cut(s) 609
Bst2BI CACGAG 1 cut(s) 78
Bst4CI ACNGT 4 cut(s) 59, 363, 517, 690
Bst6I CTCTTC 1 cut(s) 153
BstAUI TGTACA 1 cut(s) 498
BstDEI CTNAG 3 cut(s) 154, 282, 621
BstDSI CCRYGG 2 cut(s) 362, 609
BstEII GGTNACC 1 cut(s) 36
BstF5I GGATG 1 cut(s) 448
BstKTI GATC 4 cut(s) 97, 189, 205, 580
BstMAI GTCTC 3 cut(s) 155, 506, 597
BstMBI GATC 4 cut(s) 94, 186, 202, 577
BstPI GGTNACC 1 cut(s) 36
BstSFI CTRYAG 1 cut(s) 102
BstV1I GCAGC 4 cut(s) 49, 88, 116, 290
BstX2I RGATCY 1 cut(s) 94
BstYI RGATCY 1 cut(s) 94
BsuI GTATCC 1 cut(s) 501
BsuRI GGCC 1 cut(s) 346
BtgI CCRYGG 2 cut(s) 362, 609
BtsCI GGATG 1 cut(s) 448
CciI TCATGA 1 cut(s) 409
Cfr13I GGNCC 2 cut(s) 31, 344
CseI GACGC 2 cut(s) 643, 688
Csp6I GTAC 1 cut(s) 499
CviAII CATG 3 cut(s) 410, 477, 610
CviJI RGCY 6 cut(s) 107, 153, 281, 346, 508, 625
CviKI_1 RGCY 6 cut(s) 107, 153, 281, 346, 508, 625
CviQI GTAC 1 cut(s) 499
DdeI CTNAG 3 cut(s) 154, 282, 621
DpnI GATC 4 cut(s) 96, 188, 204, 579
DpnII GATC 4 cut(s) 94, 186, 202, 577
Eam1104I CTCTTC 1 cut(s) 153
EarI CTCTTC 1 cut(s) 153
Eco130I CCWWGG 1 cut(s) 609
Eco32I GATATC 1 cut(s) 483
Eco47I GGWCC 1 cut(s) 31
Eco91I GGTNACC 1 cut(s) 36
EcoO65I GGTNACC 1 cut(s) 36
EcoRI GAATTC 1 cut(s) 401
EcoRV GATATC 1 cut(s) 483
EcoT14I CCWWGG 1 cut(s) 609
ErhI CCWWGG 1 cut(s) 609
Esp3I CGTCTC 1 cut(s) 506
FaeI CATG 3 cut(s) 413, 480, 613
FaqI GGGAC 1 cut(s) 546
FatI CATG 3 cut(s) 409, 476, 609
FbaI TGATCA 1 cut(s) 202
Fnu4HI GCNGC 4 cut(s) 63, 102, 105, 279
FokI GGATG 1 cut(s) 455
Fsp4HI GCNGC 4 cut(s) 63, 102, 105, 279
FspBI CTAG 1 cut(s) 626
GluI GCNGC 4 cut(s) 63, 102, 105, 279
GsuI CTGGAG 1 cut(s) 530
HaeIII GGCC 1 cut(s) 346
HapII CCGG 1 cut(s) 34
HgaI GACGC 2 cut(s) 643, 688
Hin1II CATG 3 cut(s) 413, 480, 613
HincII GTYRAC 1 cut(s) 198
HindII GTYRAC 1 cut(s) 198
HinfI GANTC 1 cut(s) 163
HpaII CCGG 1 cut(s) 34
HphI GGTGA 4 cut(s) 30, 223, 248, 351
Hpy166II GTNNAC 3 cut(s) 55, 198, 499
Hpy188I TCNGA 4 cut(s) 186, 298, 633, 674
Hpy188III TCNNGA 3 cut(s) 167, 410, 662
Hpy8I GTNNAC 3 cut(s) 55, 198, 499
HpyAV CCTTC 1 cut(s) 191
HpyCH4III ACNGT 4 cut(s) 59, 363, 517, 690
HpyCH4V TGCA 4 cut(s) 65, 104, 317, 566
HpyF3I CTNAG 3 cut(s) 154, 282, 621
Hsp92II CATG 3 cut(s) 413, 480, 613
Ksp22I TGATCA 1 cut(s) 202
Kzo9I GATC 4 cut(s) 94, 186, 202, 577
LpnPI CCDG 9 cut(s) 47, 54, 97, 111, 261, 441, 471, 494, 609
Lsp1109I GCAGC 4 cut(s) 49, 88, 116, 290
LweI GCATC 2 cut(s) 413, 575
MaeI CTAG 1 cut(s) 626
MaeIII GTNAC 1 cut(s) 36
MalI GATC 4 cut(s) 96, 188, 204, 579
MboI GATC 4 cut(s) 94, 186, 202, 577
MboII GAAGA 3 cut(s) 120, 170, 242
MflI RGATCY 1 cut(s) 94
MluCI AATT 6 cut(s) 5, 139, 175, 318, 401, 549
MlyI GAGTC 1 cut(s) 157
MnlI CCTC 3 cut(s) 87, 389, 551
MroXI GAANNNNTTC 2 cut(s) 243, 405
MseI TTAA 4 cut(s) 117, 138, 548, 552
MslI CAYNNNNRTG 1 cut(s) 608
MspA1I CMGCKG 1 cut(s) 281
MspI CCGG 1 cut(s) 34
NcoI CCATGG 1 cut(s) 609
NdeII GATC 4 cut(s) 94, 186, 202, 577
NlaIII CATG 3 cut(s) 413, 480, 613
NlaIV GGNNCC 2 cut(s) 75, 96
NmuCI GTSAC 1 cut(s) 36
PacI TTAATTAA 1 cut(s) 552
PagI TCATGA 1 cut(s) 409
PdmI GAANNNNTTC 2 cut(s) 243, 405
PflMI CCANNNNNTGG 1 cut(s) 477
PkrI GCNGC 4 cut(s) 64, 103, 106, 280
PleI GAGTC 1 cut(s) 157
PpsI GAGTC 1 cut(s) 157
PshBI ATTAAT 1 cut(s) 548
PspEI GGTNACC 1 cut(s) 36
PspN4I GGNNCC 2 cut(s) 75, 96
PspPI GGNCC 2 cut(s) 31, 344
PstI CTGCAG 1 cut(s) 106
PsuI RGATCY 1 cut(s) 94
PvuII CAGCTG 1 cut(s) 281
RsaI GTAC 1 cut(s) 500
RsaNI GTAC 1 cut(s) 499
RseI CAYNNNNRTG 1 cut(s) 608
SaqAI TTAA 4 cut(s) 117, 138, 548, 552
SatI GCNGC 4 cut(s) 63, 102, 105, 279
Sau3AI GATC 4 cut(s) 94, 186, 202, 577
Sau96I GGNCC 2 cut(s) 31, 344
SchI GAGTC 1 cut(s) 157
SetI ASST 7 cut(s) 43, 109, 155, 183, 283, 543, 627
SfaNI GCATC 2 cut(s) 413, 575
SfcI CTRYAG 1 cut(s) 102
SinI GGWCC 1 cut(s) 31
SmiMI CAYNNNNRTG 1 cut(s) 608
SmlI CTYRAG 1 cut(s) 165
SmoI CTYRAG 1 cut(s) 165
Sse9I AATT 6 cut(s) 5, 139, 175, 318, 401, 549
SspMI CTAG 1 cut(s) 626
StyI CCWWGG 1 cut(s) 609
TaaI ACNGT 4 cut(s) 59, 363, 517, 690
TaqI TCGA 3 cut(s) 149, 421, 661
TasI AATT 6 cut(s) 5, 139, 175, 318, 401, 549
TatI WGTACW 1 cut(s) 498
Tru1I TTAA 4 cut(s) 117, 138, 548, 552
Tru9I TTAA 4 cut(s) 117, 138, 548, 552
TseFI GTSAC 1 cut(s) 36
TseI GCWGC 4 cut(s) 62, 101, 104, 278
Tsp45I GTSAC 1 cut(s) 36
TspDTI ATGAA 3 cut(s) 280, 398, 645
TspGWI ACGGA 1 cut(s) 413
Van91I CCANNNNNTGG 1 cut(s) 477
VpaK11BI GGWCC 1 cut(s) 31
VspI ATTAAT 1 cut(s) 548
XapI RAATTY 1 cut(s) 401
XmnI GAANNNNTTC 2 cut(s) 243, 405
XspI CTAG 1 cut(s) 626
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.