RchiOBHm_Chr7g0202561

flavin-containing monooxygenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0000323
Physical Location & Seq
Reverse (-)
20166964 .. 20170354
3391 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ18133

Sequence Viewer

Length: 1488 bp
ATGAAATATTTCTTAAAGAAAAGGCATATTTTGGCCGGTCAACTGTTTGGCAAAGTATGGTTGTCAATTGACCAAAATAAAACGGCGTCTGCTCTCTCTGCTCATCCGACAATGCAACCCCTATCCCGCCACGTAGCAGTGATCGGCGCCGGCGCCGCAGGCCTGGTCGCCGCACGTGAGCTCCGGCGGGAAGGTCACACTGTCGTCGTCTTCGAGCGAGGAGACCAAATCGGTGGCACGTGGGTGTACACTCCAGAAGTGGAGTCCGACCCAATTGGTCTCCACCCAAACAGAAACATAGTCCACTCGAGCATGTACCAGTCTCTCAGGACCAACCTCCCCAGAGAGATCATGGGCTTCCGGGACTACCCATTTGCGGCCAAGGAACAGGATGAAGATGGAGACCCGAGAAGGTTTCCGGGTCATAGAGAAGTGTTGAAGTACTTGAATGGTTTTGCTAAGGACTTTGGGATTTGTGAGATTGTGAGGTTTGAGAGTGAGGTGGTGTTTGTGGGTTTGGTGGAGGGTGGAAAATGGAAGGTGAAGTCCAAGAGGAAGAGAGGTGACGATATGGAGGAGGTTTATGATGCTGTGGTTGTCTGTAATGGTCATTTTACTGAGCCTCGTGTGGCCCAGATTCCAGGCATCAGCTCATGGAAAGGAAAGCAAGTTCACAGCCACAATTATCGGACTCCTGAGCCATTTCGAGATCAGGTTGTAATTTTGATAGGCAGCTCAGCTAGTGCTATTGATATTTCTCGGGACATAGCTGGAGTTGCCAAAGAAGTCCACATTGCATCTAGATCTGTTGCCACTGAAACCATGGAAAAGAAACCTGGCTATGATAACATGTGGCTTCATTCTATGATCAAAAGTGTCCATGAAGATGGTAGTGTGGTTTTCCCAGATGGGAGTGTTGTACTTGCTGACATCATTCTTCATTGCACAGGGTACAAATATCATTTCCCTTTTCTTGAAACCAATGGCATTGTAACTGTGGATGACAACCGTGTTGGGCCACTGTATAAACATATCTTTCCTCCAGCATTGGCTCCCTCACTTTCCTTTGTTGGGATACCTTTTAAGGTCGTTCCTTTTGCCATGTATGAATTTCAAAGTAAGTGGATAGCAGGTATTTTGTCGAATCGAATTGCACTTCCATCCAAAGAGGAGATGATGGAGGATGTCCAAATTTTCTGCTCGTTGCAGGAAGCTTCTGGTATACCTAAACGATTCACCCATATGATAGGTGATTACCAGTTTGAATATGATGATTGGCTTGCATCTCACTGTGGGTGTCCAGTCTCTGAGGAATGGAGACAGCAAATGTGGTTGGCCACTTTTAAGAATGCTCGTCTCCGAGGACCATTGTACCAGGAAGAGTGGGATGATCACCATTTAATATTGCAAGCTCAGGAGGACTTCAGGAAATACACCTTCAATGGAGTTAGAATGATAACTGATACAAGTGACAGACTGCCCAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004497 GO:0008150 GO:0008152 GO:0016491 GO:0055114
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FMO-like

Protein Analysis

495

Amino Acids

56.2

Weight (kDa)

6.58

Isoelectric Point (pI)

41.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FMO-like PF00743 43 - 269 7.3e-37 Flavin-binding monooxygenase-like
Pyr_redox_2 PF07992 44 - 332 2.3e-15 Pyridine nucleotide-disulphide oxidoreductase
DAO PF01266 44 - 80 9.9e-08 FAD dependent oxidoreductase
HI0933_like PF03486 44 - 78 9.5e-06 HI0933-like protein Rossmann domain
FAD_binding_2 PF00890 45 - 82 3.8e-07 FAD binding domain
FAD_oxidored PF12831 45 - 113 1.6e-06 FAD dependent oxidoreductase
NAD_binding_8 PF13450 47 - 81 1.1e-10 NAD(P)-binding Rossmann-like domain
Amino_oxidase PF01593 53 - 145 4.2e-07 Flavin containing amine oxidoreductase
Pyr_redox_3 PF13738 111 - 282 1.9e-12 Pyridine nucleotide-disulphide oxidoreductase
FMO-like PF00743 286 - 398 3.7e-15 Flavin-binding monooxygenase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000323)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G62600 AT1G62600 AT1G62620 AT1G62620 AT1G63370 AT1G63370 AT1G63390
fragaria_vesca FvH4_5g00210 FvH4_5g00210 FvH4_5g00210 FvH4_5g00210 FvH4_5g00220 FvH4_5g00220 FvH4_5g00230 FvH4_5g36500 FvH4_5g36500 FvH4_5g36500 FvH4_5g36500 FvH4_5g36530 FvH4_5g36860
malus_domestica MD06G1063000.v1.1 MD06G1063100.v1.1
prunus_persica Prupe.5G074800_v2.0.a1 Prupe.5G074900_v2.0.a1 Prupe.8G005000_v2.0.a1
pyrus_communis pycom04g07630 pycom06g06030
rosa_chinensis RchiOBHm_Chr7g0202451 RchiOBHm_Chr7g0202461 RchiOBHm_Chr7g0202471 RchiOBHm_Chr7g0202481 RchiOBHm_Chr7g0202561 RchiOBHm_Chr7g0202571 RchiOBHm_Chr7g0202581 RchiOBHm_Chr7g0202631
rosa_laevigata RLG00000003585 RLG00000003586 RLG00000003588 RLG00000003589 RLG00000003590 RLG00000003594 RLG00000003595 RLG00000003596 RLG00000003597 RLG00000003599 RLG00000003600 RLG00000003601 RLG00000003602 RLG00000029627
rosa_multiflora Rmu_co8483553.1_g000001 Rmu_co8492061.1_g000001 Rmu_sc0000611.1_g000014 Rmu_sc0000611.1_g000020 Rmu_sc0000611.1_g000021 Rmu_sc0001389.1_g000002 Rmu_sc0001389.1_g000004 Rmu_sc0002365.1_g000025 Rmu_sc0031703.1_g000001 Rmu_sc0041226.1_g000001 Rmu_sc0042472.1_g000001 Rmu_sc0042472.1_g000002
rosa_roxburghii Rroxscaffold_3G00254490 Rroxscaffold_3G00254500 Rroxscaffold_3G00254510 Rroxscaffold_3G00254570 Rroxscaffold_3G00254580 Rroxscaffold_3G00254590 Rroxscaffold_3G00254600
rosa_rugosa Rorug07G0070700 Rorug07G0070800 Rorug07G0070900 Rorug07G0071000 Rorug07G0071100 Rorug07G0071300 Rorug07G0071400
rosa_samantha Rh7AG198100 Rh7AG198200 Rh7AG198300 Rh7AG198400 Rh7AG198700 Rh7AG198800 Rh7AG198900 Rh7AG199000 Rh7AG199100 Rh7AG199200 Rh7AG199400 Rh7BG199400 Rh7BG199500 Rh7BG199600 Rh7BG199700 Rh7BG200000 Rh7BG200100 Rh7CG209200 Rh7CG209300 Rh7CG209400 Rh7CG209700 Rh7CG210300 Rh7CG210400 Rh7CG210500 Rh7CG210900 Rh7DG205000 Rh7DG205100 Rh7DG205200 Rh7DG205300 Rh7DG205800 Rh7DG205900 Rh7DG206000 Rh7DG206100 Rh7DG206300
rosa_wichuraiana Rw7G017380 Rw7G017390 Rw7G017400 Rw7G017440 Rw7G017460

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1121
AccB1I GGYRCC 2 cut(s) 146, 152
AccI GTMKAC 1 cut(s) 1222
AciI CCGC 5 cut(s) 127, 156, 171, 187, 377
AcoI YGGCCR 3 cut(s) 33, 378, 1335
AcsI RAATTY 2 cut(s) 1109, 1191
AcuI CTGAAG 1 cut(s) 1408
AcvI CACGTG 2 cut(s) 176, 240
AcyI GRCGYC 3 cut(s) 86, 147, 153
AfaI GTAC 6 cut(s) 248, 317, 443, 921, 953, 1373
AfiI CCNNNNNNNGG 2 cut(s) 376, 1071
AflIII ACRYGT 1 cut(s) 849
AgsI TTSAA 6 cut(s) 439, 448, 977, 1115, 1265, 1441
AjnI CCWGG 4 cut(s) 162, 640, 835, 1374
AleI CACNNNNGTG 1 cut(s) 242
AloI GAACNNNNNNTCC 2 cut(s) 654, 686
AluBI AGCT 7 cut(s) 181, 651, 735, 740, 770, 1214, 1412
AluI AGCT 7 cut(s) 181, 651, 735, 740, 770, 1214, 1412
Alw21I GWGCWC 1 cut(s) 183
Alw26I GTCTC 7 cut(s) 216, 284, 327, 396, 1309, 1312, 1361
AlwNI CAGNNNCTG 1 cut(s) 1307
Ama87I CYCGRG 3 cut(s) 307, 406, 759
AoxI GGCC 6 cut(s) 33, 160, 378, 630, 1016, 1335
ApeKI GCWGC 1 cut(s) 732
ApoI RAATTY 2 cut(s) 1109, 1191
ArsI GACNNNNNNTTYG 2 cut(s) 356, 388
Asp700I GAANNNNTTC 1 cut(s) 8
AspLEI GCGC 2 cut(s) 149, 155
AspS9I GGNCC 4 cut(s) 330, 631, 1016, 1364
AsuC2I CCSGG 2 cut(s) 362, 420
AsuHPI GGTGA 5 cut(s) 553, 575, 1228, 1262, 1385
AvaI CYCGRG 3 cut(s) 307, 406, 759
AvaII GGWCC 2 cut(s) 330, 1364
BaeI ACNNNNGTAYC 2 cut(s) 1355, 1388
BalI TGGCCA 1 cut(s) 1337
BanI GGYRCC 2 cut(s) 146, 152
BanII GRGCYC 1 cut(s) 183
BauI CACGAG 1 cut(s) 624
BbrPI CACGTG 2 cut(s) 176, 240
BbsI GAAGAC 1 cut(s) 202
Bbv12I GWGCWC 1 cut(s) 183
BbvI GCAGC 1 cut(s) 744
BccI CCATC 5 cut(s) 392, 881, 902, 1168, 1171
BceAI ACGGC 1 cut(s) 99
BciT130I CCWGG 4 cut(s) 164, 642, 837, 1376
BciVI GTATCC 1 cut(s) 1068
BclI TGATCA 2 cut(s) 867, 1390
BcnI CCSGG 2 cut(s) 362, 420
BcoDI GTCTC 7 cut(s) 216, 284, 327, 396, 1309, 1312, 1361
BfaI CTAG 2 cut(s) 741, 801
BfoI RGCGCY 2 cut(s) 150, 156
BfuAI ACCTGC 1 cut(s) 1121
BfuI GTATCC 1 cut(s) 1068
BglII AGATCT 1 cut(s) 803
BisI GCNGC 4 cut(s) 156, 171, 378, 733
BlpI GCTNAGC 1 cut(s) 736
BlsI GCNGC 4 cut(s) 157, 172, 379, 734
BmcAI AGTACT 1 cut(s) 443
Bme1390I CCNGG 6 cut(s) 164, 362, 420, 642, 837, 1376
Bme18I GGWCC 2 cut(s) 330, 1364
BmeT110I CYCGRG 3 cut(s) 307, 406, 759
BmgT120I GGNCC 4 cut(s) 330, 631, 1016, 1364
BmiI GGNNCC 3 cut(s) 148, 154, 1053
BmrFI CCNGG 6 cut(s) 164, 362, 420, 642, 837, 1376
BmsI GCATC 4 cut(s) 577, 654, 806, 1292
BpiI GAAGAC 1 cut(s) 202
BpmI CTGGAG 3 cut(s) 237, 792, 1026
Bpu10I CCTNAGC 3 cut(s) 459, 696, 1413
Bpu1102I GCTNAGC 1 cut(s) 736
BpuMI CCSGG 2 cut(s) 362, 420
BsaAI YACGTR 3 cut(s) 133, 176, 240
BsaHI GRCGYC 3 cut(s) 86, 147, 153
BsaI GGTCTC 3 cut(s) 216, 284, 396
BsaJI CCNNGG 3 cut(s) 381, 822, 1360
BsaXI ACNNNNNCTCC 2 cut(s) 165, 195
Bsc4I CCNNNNNNNGG 2 cut(s) 376, 1071
Bse118I RCCGGY 2 cut(s) 35, 149
Bse1I ACTGG 3 cut(s) 319, 1258, 1301
Bse3DI GCAATG 2 cut(s) 792, 940
BseBI CCWGG 4 cut(s) 164, 642, 837, 1376
BseDI CCNNGG 3 cut(s) 381, 822, 1360
BseGI GGATG 6 cut(s) 103, 397, 1006, 1160, 1189, 1393
BseLI CCNNNNNNNGG 2 cut(s) 376, 1071
BseMI GCAATG 2 cut(s) 792, 940
BseMII CTCAG 6 cut(s) 340, 609, 687, 750, 1299, 1427
BseNI ACTGG 3 cut(s) 319, 1258, 1301
BseRI GAGGAG 3 cut(s) 234, 590, 1184
BseXI GCAGC 1 cut(s) 744
BshFI GGCC 6 cut(s) 35, 162, 380, 632, 1018, 1337
BshNI GGYRCC 2 cut(s) 146, 152
BsiHKAI GWGCWC 1 cut(s) 183
BsiHKCI CYCGRG 3 cut(s) 307, 406, 759
BsiSI CCGG 5 cut(s) 36, 150, 184, 361, 419
BslFI GGGAC 2 cut(s) 377, 776
BslI CCNNNNNNNGG 2 cut(s) 376, 1071
BsmAI GTCTC 7 cut(s) 216, 284, 327, 396, 1309, 1312, 1361
BsmBI CGTCTC 1 cut(s) 1361
BsmFI GGGAC 2 cut(s) 377, 776
BsmI GAATGC 1 cut(s) 1354
BsnI GGCC 6 cut(s) 35, 162, 380, 632, 1018, 1337
Bso31I GGTCTC 3 cut(s) 216, 284, 396
BsoBI CYCGRG 3 cut(s) 307, 406, 759
Bsp1286I GDGCHC 1 cut(s) 183
Bsp1407I TGTACA 1 cut(s) 246
Bsp143I GATC 6 cut(s) 141, 348, 709, 803, 867, 1390
Bsp1720I GCTNAGC 1 cut(s) 736
Bsp19I CCATGG 1 cut(s) 822
BspACI CCGC 5 cut(s) 127, 156, 171, 187, 377
BspANI GGCC 6 cut(s) 35, 162, 380, 632, 1018, 1337
BspCNI CTCAG 6 cut(s) 339, 610, 688, 749, 1300, 1426
BspLI GGNNCC 3 cut(s) 148, 154, 1053
BspMI ACCTGC 1 cut(s) 1121
BspT107I GGYRCC 2 cut(s) 146, 152
BspTNI GGTCTC 3 cut(s) 216, 284, 396
BsrDI GCAATG 2 cut(s) 792, 940
BsrFI RCCGGY 2 cut(s) 35, 149
BsrGI TGTACA 1 cut(s) 246
BsrI ACTGG 3 cut(s) 319, 1258, 1301
BssAI RCCGGY 2 cut(s) 35, 149
BssECI CCNNGG 3 cut(s) 381, 822, 1360
BssMI GATC 6 cut(s) 141, 348, 709, 803, 867, 1390
BssNAI GTATAC 1 cut(s) 1223
BssNI GRCGYC 3 cut(s) 86, 147, 153
BssSI CACGAG 1 cut(s) 624
BssT1I CCWWGG 2 cut(s) 381, 822
Bst1107I GTATAC 1 cut(s) 1223
Bst2BI CACGAG 1 cut(s) 624
Bst2UI CCWGG 4 cut(s) 164, 642, 837, 1376
Bst4CI ACNGT 6 cut(s) 45, 202, 997, 1010, 1023, 1292
Bst6I CTCTTC 2 cut(s) 551, 1374
BstACI GRCGYC 3 cut(s) 86, 147, 153
BstAUI TGTACA 1 cut(s) 246
BstBAI YACGTR 3 cut(s) 133, 176, 240
BstC8I GCNNGC 4 cut(s) 151, 160, 1281, 1410
BstDEI CTNAG 7 cut(s) 326, 459, 618, 696, 736, 1308, 1413
BstDSI CCRYGG 1 cut(s) 822
BstF5I GGATG 6 cut(s) 103, 397, 1006, 1160, 1189, 1393
BstH2I RGCGCY 2 cut(s) 150, 156
BstHHI GCGC 2 cut(s) 149, 155
BstKTI GATC 6 cut(s) 144, 351, 712, 806, 870, 1393
BstMAI GTCTC 7 cut(s) 216, 284, 327, 396, 1309, 1312, 1361
BstMBI GATC 6 cut(s) 141, 348, 709, 803, 867, 1390
BstMWI GCNNNNNNNGC 4 cut(s) 98, 155, 159, 776
BstNI CCWGG 4 cut(s) 164, 642, 837, 1376
BstNSI RCATGY 2 cut(s) 316, 853
BstSCI CCNGG 6 cut(s) 162, 360, 418, 640, 835, 1374
BstV1I GCAGC 1 cut(s) 744
BstV2I GAAGAC 1 cut(s) 202
BstX2I RGATCY 1 cut(s) 803
BstXI CCANNNNNNTGG 2 cut(s) 233, 887
BstYI RGATCY 1 cut(s) 803
BstZ17I GTATAC 1 cut(s) 1223
BsuI GTATCC 1 cut(s) 1068
BsuRI GGCC 6 cut(s) 35, 162, 380, 632, 1018, 1337
BtgI CCRYGG 1 cut(s) 822
BtsCI GGATG 6 cut(s) 103, 397, 1006, 1160, 1189, 1393
BtsI GCAGTG 1 cut(s) 144
BtsIMutI CAGTG 5 cut(s) 144, 198, 813, 1019, 1288
BveI ACCTGC 1 cut(s) 1121
Cac8I GCNNGC 4 cut(s) 151, 160, 1281, 1410
CaiI CAGNNNCTG 1 cut(s) 1307
CfoI GCGC 2 cut(s) 149, 155
Cfr10I RCCGGY 2 cut(s) 35, 149
Cfr13I GGNCC 4 cut(s) 330, 631, 1016, 1364
CseI GACGC 1 cut(s) 75
Csp6I GTAC 6 cut(s) 247, 316, 442, 920, 952, 1372
CspCI CAANNNNNGTGG 2 cut(s) 1103, 1138
CviAII CATG 7 cut(s) 313, 352, 654, 823, 850, 881, 1102
CviQI GTAC 6 cut(s) 247, 316, 442, 920, 952, 1372
DdeI CTNAG 7 cut(s) 326, 459, 618, 696, 736, 1308, 1413
DinI GGCGCC 2 cut(s) 148, 154
DpnI GATC 6 cut(s) 143, 350, 711, 805, 869, 1392
DpnII GATC 6 cut(s) 141, 348, 709, 803, 867, 1390
EaeI YGGCCR 3 cut(s) 33, 378, 1335
Eam1104I CTCTTC 2 cut(s) 551, 1374
EarI CTCTTC 2 cut(s) 551, 1374
Ecl136II GAGCTC 1 cut(s) 181
Eco130I CCWWGG 2 cut(s) 381, 822
Eco147I AGGCCT 1 cut(s) 162
Eco24I GRGCYC 1 cut(s) 183
Eco31I GGTCTC 3 cut(s) 216, 284, 396
Eco47I GGWCC 2 cut(s) 330, 1364
Eco53kI GAGCTC 1 cut(s) 181
Eco57I CTGAAG 1 cut(s) 1408
Eco72I CACGTG 2 cut(s) 176, 240
Eco88I CYCGRG 3 cut(s) 307, 406, 759
EcoICRI GAGCTC 1 cut(s) 181
EcoRII CCWGG 4 cut(s) 162, 640, 835, 1374
EcoT14I CCWWGG 2 cut(s) 381, 822
EcoT38I GRGCYC 1 cut(s) 183
EgeI GGCGCC 2 cut(s) 148, 154
EheI GGCGCC 2 cut(s) 148, 154
ErhI CCWWGG 2 cut(s) 381, 822
Esp3I CGTCTC 1 cut(s) 1361
FaeI CATG 7 cut(s) 316, 355, 657, 826, 853, 884, 1105
FaqI GGGAC 2 cut(s) 377, 776
FatI CATG 7 cut(s) 312, 351, 653, 822, 849, 880, 1101
FauI CCCGC 2 cut(s) 134, 180
FauNDI CATATG 1 cut(s) 1242
FbaI TGATCA 2 cut(s) 867, 1390
FblI GTMKAC 1 cut(s) 1222
Fnu4HI GCNGC 4 cut(s) 156, 171, 378, 733
FokI GGATG 6 cut(s) 90, 404, 1013, 1147, 1196, 1400
FriOI GRGCYC 1 cut(s) 183
Fsp4HI GCNGC 4 cut(s) 156, 171, 378, 733
FspBI CTAG 2 cut(s) 741, 801
GlaI GCGC 2 cut(s) 148, 154
GluI GCNGC 4 cut(s) 156, 171, 378, 733
GsuI CTGGAG 3 cut(s) 237, 792, 1026
HaeII RGCGCY 2 cut(s) 150, 156
HaeIII GGCC 6 cut(s) 35, 162, 380, 632, 1018, 1337
HapII CCGG 5 cut(s) 36, 150, 184, 361, 419
HgaI GACGC 1 cut(s) 75
HhaI GCGC 2 cut(s) 149, 155
Hin1I GRCGYC 3 cut(s) 86, 147, 153
Hin1II CATG 7 cut(s) 316, 355, 657, 826, 853, 884, 1105
Hin6I GCGC 2 cut(s) 147, 153
HinP1I GCGC 2 cut(s) 147, 153
HincII GTYRAC 1 cut(s) 41
HindII GTYRAC 1 cut(s) 41
HindIII AAGCTT 1 cut(s) 1212
HinfI GANTC 5 cut(s) 263, 637, 691, 1144, 1233
HpaII CCGG 5 cut(s) 36, 150, 184, 361, 419
HphI GGTGA 5 cut(s) 553, 575, 1228, 1262, 1385
Hpy166II GTNNAC 7 cut(s) 41, 247, 249, 304, 673, 790, 1223
Hpy188I TCNGA 5 cut(s) 108, 268, 690, 1309, 1361
Hpy188III TCNNGA 9 cut(s) 254, 328, 695, 707, 761, 801, 974, 1415, 1426
Hpy8I GTNNAC 7 cut(s) 41, 247, 249, 304, 673, 790, 1223
Hpy99I CGWCG 1 cut(s) 209
HpyAV CCTTC 4 cut(s) 185, 405, 532, 1447
HpyCH4III ACNGT 6 cut(s) 45, 202, 997, 1010, 1023, 1292
HpyCH4IV ACGT 3 cut(s) 132, 175, 239
HpyCH4V TGCA 7 cut(s) 115, 797, 945, 1154, 1207, 1283, 1408
HpyF10VI GCNNNNNNNGC 4 cut(s) 98, 155, 159, 776
HpyF3I CTNAG 7 cut(s) 326, 459, 618, 696, 736, 1308, 1413
HpySE526I ACGT 3 cut(s) 132, 175, 239
Hsp92I GRCGYC 3 cut(s) 86, 147, 153
Hsp92II CATG 7 cut(s) 316, 355, 657, 826, 853, 884, 1105
HspAI GCGC 2 cut(s) 147, 153
KasI GGCGCC 2 cut(s) 146, 152
KroI GCCGGC 1 cut(s) 149
KroNI GCCGGC 1 cut(s) 151
Ksp22I TGATCA 2 cut(s) 867, 1390
Kzo9I GATC 6 cut(s) 141, 348, 709, 803, 867, 1390
LmnI GCTCC 2 cut(s) 186, 1057
Lsp1109I GCAGC 1 cut(s) 744
LweI GCATC 4 cut(s) 577, 654, 806, 1292
MaeI CTAG 2 cut(s) 741, 801
MaeII ACGT 3 cut(s) 132, 175, 239
MaeIII GTNAC 4 cut(s) 194, 563, 991, 1469
MalI GATC 6 cut(s) 143, 350, 711, 805, 869, 1392
MboI GATC 6 cut(s) 141, 348, 709, 803, 867, 1390
MboII GAAGA 6 cut(s) 202, 407, 568, 896, 929, 1391
MfeI CAATTG 2 cut(s) 66, 273
MflI RGATCY 1 cut(s) 803
MhlI GDGCHC 1 cut(s) 183
MlsI TGGCCA 1 cut(s) 1337
MluCI AATT 7 cut(s) 66, 273, 682, 720, 1109, 1149, 1191
MluNI TGGCCA 1 cut(s) 1337
Mly113I GGCGCC 2 cut(s) 147, 153
MlyI GAGTC 2 cut(s) 272, 685
MmeI TCCRAC 2 cut(s) 131, 291
Mox20I TGGCCA 1 cut(s) 1337
MreI CGCCGGCG 1 cut(s) 149
MroNI GCCGGC 1 cut(s) 149
MroXI GAANNNNTTC 1 cut(s) 8
MscI TGGCCA 1 cut(s) 1337
MseI TTAA 4 cut(s) 14, 1083, 1344, 1400
MslI CAYNNNNRTG 3 cut(s) 242, 885, 1241
Msp20I TGGCCA 1 cut(s) 1337
MspI CCGG 5 cut(s) 36, 150, 184, 361, 419
MspR9I CCNGG 6 cut(s) 164, 362, 420, 642, 837, 1376
MunI CAATTG 2 cut(s) 66, 273
Mva1269I GAATGC 1 cut(s) 1354
MvaI CCWGG 4 cut(s) 164, 642, 837, 1376
MwoI GCNNNNNNNGC 4 cut(s) 98, 155, 159, 776
NaeI GCCGGC 1 cut(s) 151
NarI GGCGCC 2 cut(s) 147, 153
NciI CCSGG 2 cut(s) 362, 420
NcoI CCATGG 1 cut(s) 822
NdeI CATATG 1 cut(s) 1242
NdeII GATC 6 cut(s) 141, 348, 709, 803, 867, 1390
NgoMIV GCCGGC 1 cut(s) 149
NlaIII CATG 7 cut(s) 316, 355, 657, 826, 853, 884, 1105
NlaIV GGNNCC 3 cut(s) 148, 154, 1053
NmuCI GTSAC 3 cut(s) 194, 563, 1469
NspI RCATGY 2 cut(s) 316, 853
OliI CACNNNNGTG 1 cut(s) 242
PaeR7I CTCGAG 1 cut(s) 307
PceI AGGCCT 1 cut(s) 162
PciI ACATGT 1 cut(s) 849
PcsI WCGNNNNNNNCGW 1 cut(s) 210
PctI GAATGC 1 cut(s) 1354
PdiI GCCGGC 1 cut(s) 151
PdmI GAANNNNTTC 1 cut(s) 8
PfeI GAWTC 3 cut(s) 637, 1144, 1233
PfoI TCCNGGA 1 cut(s) 360
PkrI GCNGC 4 cut(s) 157, 172, 379, 734
PleI GAGTC 2 cut(s) 271, 685
PluTI GGCGCC 2 cut(s) 150, 156
PmaCI CACGTG 2 cut(s) 176, 240
PmlI CACGTG 2 cut(s) 176, 240
PpsI GAGTC 2 cut(s) 271, 685
Ppu21I YACGTR 3 cut(s) 133, 176, 240
PscI ACATGT 1 cut(s) 849
Psp124BI GAGCTC 1 cut(s) 183
Psp6I CCWGG 4 cut(s) 162, 640, 835, 1374
PspCI CACGTG 2 cut(s) 176, 240
PspGI CCWGG 4 cut(s) 162, 640, 835, 1374
PspN4I GGNNCC 3 cut(s) 148, 154, 1053
PspPI GGNCC 4 cut(s) 330, 631, 1016, 1364
PspXI VCTCGAGB 1 cut(s) 307
PstNI CAGNNNCTG 1 cut(s) 1307
PsuI RGATCY 1 cut(s) 803
RsaI GTAC 6 cut(s) 248, 317, 443, 921, 953, 1373
RsaNI GTAC 6 cut(s) 247, 316, 442, 920, 952, 1372
RseI CAYNNNNRTG 3 cut(s) 242, 885, 1241
SacI GAGCTC 1 cut(s) 183
SaqAI TTAA 4 cut(s) 14, 1083, 1344, 1400
SatI GCNGC 4 cut(s) 156, 171, 378, 733
Sau3AI GATC 6 cut(s) 141, 348, 709, 803, 867, 1390
Sau96I GGNCC 4 cut(s) 330, 631, 1016, 1364
ScaI AGTACT 1 cut(s) 443
SchI GAGTC 2 cut(s) 272, 685
ScrFI CCNGG 6 cut(s) 164, 362, 420, 642, 837, 1376
SduI GDGCHC 1 cut(s) 183
SfaNI GCATC 4 cut(s) 577, 654, 806, 1292
SfoI GGCGCC 2 cut(s) 148, 154
Sfr274I CTCGAG 1 cut(s) 307
SgrAI CRCCGGYG 1 cut(s) 149
SinI GGWCC 2 cut(s) 330, 1364
SlaI CTCGAG 1 cut(s) 307
SmiMI CAYNNNNRTG 3 cut(s) 242, 885, 1241
SmlI CTYRAG 1 cut(s) 307
SmoI CTYRAG 1 cut(s) 307
Sse9I AATT 7 cut(s) 66, 273, 682, 720, 1109, 1149, 1191
SseBI AGGCCT 1 cut(s) 162
SsiI CCGC 5 cut(s) 127, 156, 171, 187, 377
SspDI GGCGCC 2 cut(s) 146, 152
SspI AATATT 2 cut(s) 8, 1404
SspMI CTAG 2 cut(s) 741, 801
SstI GAGCTC 1 cut(s) 183
StuI AGGCCT 1 cut(s) 162
StyD4I CCNGG 6 cut(s) 162, 360, 418, 640, 835, 1374
StyI CCWWGG 2 cut(s) 381, 822
TaaI ACNGT 6 cut(s) 45, 202, 997, 1010, 1023, 1292
TaiI ACGT 3 cut(s) 135, 178, 242
TaqI TCGA 5 cut(s) 213, 308, 706, 1142, 1147
TasI AATT 7 cut(s) 66, 273, 682, 720, 1109, 1149, 1191
TatI WGTACW 3 cut(s) 246, 441, 919
TauI GCSGC 3 cut(s) 158, 173, 380
TfiI GAWTC 3 cut(s) 637, 1144, 1233
Tru1I TTAA 4 cut(s) 14, 1083, 1344, 1400
Tru9I TTAA 4 cut(s) 14, 1083, 1344, 1400
TscAI CASTG 5 cut(s) 144, 205, 820, 1026, 1295
TseFI GTSAC 3 cut(s) 194, 563, 1469
TseI GCWGC 1 cut(s) 732
Tsp45I GTSAC 3 cut(s) 194, 563, 1469
TspDTI ATGAA 6 cut(s) 17, 408, 848, 897, 929, 1122
TspRI CASTG 5 cut(s) 144, 205, 820, 1026, 1295
VpaK11BI GGWCC 2 cut(s) 330, 1364
XapI RAATTY 2 cut(s) 1109, 1191
XbaI TCTAGA 1 cut(s) 800
XceI RCATGY 2 cut(s) 316, 853
XcmI CCANNNNNNNNNTGG 2 cut(s) 349, 820
XhoI CTCGAG 1 cut(s) 307
XmiI GTMKAC 1 cut(s) 1222
XmnI GAANNNNTTC 1 cut(s) 8
XspI CTAG 2 cut(s) 741, 801
ZrmI AGTACT 1 cut(s) 443
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.