Serine threonine-protein kinase
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 2 cut(s) | 674, 2681 |
| Acc36I | ACCTGC | 1 cut(s) | 1529 |
| Acc65I | GGTACC | 1 cut(s) | 640 |
| AccB1I | GGYRCC | 1 cut(s) | 640 |
| AccBSI | CCGCTC | 2 cut(s) | 38, 107 |
| AccII | CGCG | 2 cut(s) | 138, 830 |
| AclI | AACGTT | 1 cut(s) | 441 |
| AclWI | GGATC | 2 cut(s) | 467, 2124 |
| AcoI | YGGCCR | 1 cut(s) | 1354 |
| AcsI | RAATTY | 4 cut(s) | 968, 1289, 1508, 2486 |
| AcuI | CTGAAG | 3 cut(s) | 1052, 1800, 2722 |
| AdeI | CACNNNGTG | 2 cut(s) | 2537, 2576 |
| AfaI | GTAC | 4 cut(s) | 642, 1519, 1614, 2344 |
| AfiI | CCNNNNNNNGG | 8 cut(s) | 61, 196, 396, 1330, 1855, 1967, 2751, 2796 |
| AflII | CTTAAG | 1 cut(s) | 1513 |
| AjiI | CACGTC | 1 cut(s) | 2612 |
| AjnI | CCWGG | 4 cut(s) | 195, 2081, 2151, 2395 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 585, 617 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 1749, 1781 |
| AluBI | AGCT | 7 cut(s) | 211, 336, 1186, 1907, 2129, 2447, 2742 |
| AluI | AGCT | 7 cut(s) | 211, 336, 1186, 1907, 2129, 2447, 2742 |
| Alw21I | GWGCWC | 1 cut(s) | 268 |
| Alw26I | GTCTC | 1 cut(s) | 759 |
| AlwI | GGATC | 2 cut(s) | 467, 2124 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 2174, 2351 |
| Ama87I | CYCGRG | 1 cut(s) | 626 |
| AoxI | GGCC | 5 cut(s) | 7, 421, 1222, 1354, 2186 |
| ApoI | RAATTY | 4 cut(s) | 968, 1289, 1508, 2486 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 2086, 2118 |
| Asp718I | GGTACC | 1 cut(s) | 640 |
| AspLEI | GCGC | 4 cut(s) | 140, 364, 389, 1603 |
| AspS9I | GGNCC | 4 cut(s) | 399, 646, 1222, 2393 |
| AsuHPI | GGTGA | 5 cut(s) | 247, 1414, 2267, 2562, 2647 |
| AvaI | CYCGRG | 1 cut(s) | 626 |
| AvaII | GGWCC | 3 cut(s) | 399, 646, 2393 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 2163, 2196 |
| BalI | TGGCCA | 1 cut(s) | 1356 |
| BanI | GGYRCC | 1 cut(s) | 640 |
| BanII | GRGCYC | 1 cut(s) | 2067 |
| BbsI | GAAGAC | 5 cut(s) | 316, 1487, 1851, 2096, 2369 |
| Bbv12I | GWGCWC | 1 cut(s) | 268 |
| BccI | CCATC | 3 cut(s) | 389, 1226, 2301 |
| BceAI | ACGGC | 2 cut(s) | 352, 436 |
| BcgI | CGANNNNNNTGC | 4 cut(s) | 452, 486, 1152, 1186 |
| BciT130I | CCWGG | 4 cut(s) | 197, 2083, 2153, 2397 |
| BcoDI | GTCTC | 1 cut(s) | 759 |
| BfaI | CTAG | 8 cut(s) | 32, 582, 1208, 1344, 1805, 2141, 2535, 2718 |
| BfmI | CTRYAG | 3 cut(s) | 1821, 2332, 2412 |
| BfoI | RGCGCY | 2 cut(s) | 365, 390 |
| BfrI | CTTAAG | 1 cut(s) | 1513 |
| BfuAI | ACCTGC | 1 cut(s) | 1529 |
| BglII | AGATCT | 2 cut(s) | 1783, 2191 |
| BlpI | GCTNAGC | 1 cut(s) | 374 |
| Bme1390I | CCNGG | 4 cut(s) | 197, 2083, 2153, 2397 |
| Bme18I | GGWCC | 3 cut(s) | 399, 646, 2393 |
| BmeT110I | CYCGRG | 1 cut(s) | 626 |
| BmgBI | CACGTC | 1 cut(s) | 2612 |
| BmgT120I | GGNCC | 4 cut(s) | 399, 646, 1222, 2393 |
| BmiI | GGNNCC | 5 cut(s) | 400, 642, 802, 1758, 2562 |
| BmrFI | CCNGG | 4 cut(s) | 197, 2083, 2153, 2397 |
| BmrI | ACTGGG | 1 cut(s) | 1722 |
| BmuI | ACTGGG | 1 cut(s) | 1722 |
| BpiI | GAAGAC | 5 cut(s) | 316, 1487, 1851, 2096, 2369 |
| BplI | GAGNNNNNCTC | 6 cut(s) | 506, 538, 2223, 2255, 2225, 2257 |
| BpmI | CTGGAG | 2 cut(s) | 1208, 2547 |
| Bpu10I | CCTNAGC | 1 cut(s) | 917 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 374 |
| BpuEI | CTTGAG | 3 cut(s) | 527, 547, 2427 |
| BsaBI | GATNNNNATC | 2 cut(s) | 949, 2311 |
| BsaJI | CCNNGG | 5 cut(s) | 60, 195, 196, 932, 2031 |
| BsaWI | WCCGGW | 1 cut(s) | 643 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 86, 116, 1175, 1205 |
| Bsc4I | CCNNNNNNNGG | 8 cut(s) | 61, 196, 396, 1330, 1855, 1967, 2751, 2796 |
| Bse1I | ACTGG | 6 cut(s) | 1717, 1884, 1984, 2168, 2562, 2777 |
| Bse3DI | GCAATG | 1 cut(s) | 1365 |
| Bse8I | GATNNNNATC | 2 cut(s) | 949, 2311 |
| BseBI | CCWGG | 4 cut(s) | 197, 2083, 2153, 2397 |
| BseDI | CCNNGG | 5 cut(s) | 60, 195, 196, 932, 2031 |
| BseJI | GATNNNNATC | 2 cut(s) | 949, 2311 |
| BseLI | CCNNNNNNNGG | 8 cut(s) | 61, 196, 396, 1330, 1855, 1967, 2751, 2796 |
| BseMI | GCAATG | 1 cut(s) | 1365 |
| BseMII | CTCAG | 3 cut(s) | 221, 1079, 1339 |
| BseNI | ACTGG | 6 cut(s) | 1717, 1884, 1984, 2168, 2562, 2777 |
| BseRI | GAGGAG | 2 cut(s) | 155, 1196 |
| BseYI | CCCAGC | 2 cut(s) | 2111, 2210 |
| Bsh1236I | CGCG | 2 cut(s) | 138, 830 |
| BshFI | GGCC | 5 cut(s) | 9, 423, 1224, 1356, 2188 |
| BshNI | GGYRCC | 1 cut(s) | 640 |
| BsiHKAI | GWGCWC | 1 cut(s) | 268 |
| BsiHKCI | CYCGRG | 1 cut(s) | 626 |
| BsiSI | CCGG | 2 cut(s) | 390, 644 |
| BslFI | GGGAC | 2 cut(s) | 412, 722 |
| BslI | CCNNNNNNNGG | 8 cut(s) | 61, 196, 396, 1330, 1855, 1967, 2751, 2796 |
| BsmAI | GTCTC | 1 cut(s) | 759 |
| BsmFI | GGGAC | 2 cut(s) | 412, 722 |
| BsnI | GGCC | 5 cut(s) | 9, 423, 1224, 1356, 2188 |
| BsoBI | CYCGRG | 1 cut(s) | 626 |
| Bsp1286I | GDGCHC | 2 cut(s) | 268, 2067 |
| Bsp143I | GATC | 5 cut(s) | 459, 616, 1783, 2116, 2191 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 374 |
| BspANI | GGCC | 5 cut(s) | 9, 423, 1224, 1356, 2188 |
| BspCNI | CTCAG | 3 cut(s) | 220, 1078, 1338 |
| BspFNI | CGCG | 2 cut(s) | 138, 830 |
| BspLI | GGNNCC | 5 cut(s) | 400, 642, 802, 1758, 2562 |
| BspMAI | CTGCAG | 2 cut(s) | 1825, 2336 |
| BspMI | ACCTGC | 1 cut(s) | 1529 |
| BspPI | GGATC | 2 cut(s) | 467, 2124 |
| BspQI | GCTCTTC | 1 cut(s) | 45 |
| BspT107I | GGYRCC | 1 cut(s) | 640 |
| BspTI | CTTAAG | 1 cut(s) | 1513 |
| BsrBI | CCGCTC | 2 cut(s) | 38, 107 |
| BsrDI | GCAATG | 1 cut(s) | 1365 |
| BsrI | ACTGG | 6 cut(s) | 1717, 1884, 1984, 2168, 2562, 2777 |
| BssECI | CCNNGG | 5 cut(s) | 60, 195, 196, 932, 2031 |
| BssMI | GATC | 5 cut(s) | 459, 616, 1783, 2116, 2191 |
| BssT1I | CCWWGG | 2 cut(s) | 60, 2031 |
| Bst2UI | CCWGG | 4 cut(s) | 197, 2083, 2153, 2397 |
| Bst4CI | ACNGT | 7 cut(s) | 693, 853, 1501, 1692, 1802, 1888, 2835 |
| Bst6I | CTCTTC | 2 cut(s) | 45, 79 |
| BstAFI | CTTAAG | 1 cut(s) | 1513 |
| BstAPI | GCANNNNNTGC | 2 cut(s) | 263, 2096 |
| BstC8I | GCNNGC | 5 cut(s) | 425, 493, 828, 2014, 2088 |
| BstDEI | CTNAG | 5 cut(s) | 207, 374, 917, 1065, 1325 |
| BstENI | CCTNNNNNAGG | 3 cut(s) | 59, 194, 1853 |
| BstFNI | CGCG | 2 cut(s) | 138, 830 |
| BstH2I | RGCGCY | 2 cut(s) | 365, 390 |
| BstHHI | GCGC | 4 cut(s) | 140, 364, 389, 1603 |
| BstKTI | GATC | 5 cut(s) | 462, 619, 1786, 2119, 2194 |
| BstMAI | GTCTC | 1 cut(s) | 759 |
| BstMBI | GATC | 5 cut(s) | 459, 616, 1783, 2116, 2191 |
| BstNI | CCWGG | 4 cut(s) | 197, 2083, 2153, 2397 |
| BstNSI | RCATGY | 3 cut(s) | 1248, 1723, 2714 |
| BstSCI | CCNGG | 4 cut(s) | 195, 2081, 2151, 2395 |
| BstSFI | CTRYAG | 3 cut(s) | 1821, 2332, 2412 |
| BstUI | CGCG | 2 cut(s) | 138, 830 |
| BstV2I | GAAGAC | 5 cut(s) | 316, 1487, 1851, 2096, 2369 |
| BstX2I | RGATCY | 3 cut(s) | 1783, 2116, 2191 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 1232 |
| BstYI | RGATCY | 3 cut(s) | 1783, 2116, 2191 |
| BsuRI | GGCC | 5 cut(s) | 9, 423, 1224, 1356, 2188 |
| BtrI | CACGTC | 1 cut(s) | 2612 |
| BtsI | GCAGTG | 1 cut(s) | 864 |
| BtsIMutI | CAGTG | 7 cut(s) | 864, 1163, 1333, 1877, 2596, 2666, 2784 |
| BveI | ACCTGC | 1 cut(s) | 1529 |
| Cac8I | GCNNGC | 5 cut(s) | 425, 493, 828, 2014, 2088 |
| CaiI | CAGNNNCTG | 2 cut(s) | 2174, 2351 |
| CfoI | GCGC | 4 cut(s) | 140, 364, 389, 1603 |
| Cfr13I | GGNCC | 4 cut(s) | 399, 646, 1222, 2393 |
| CsiI | ACCWGGT | 1 cut(s) | 2395 |
| Csp6I | GTAC | 4 cut(s) | 641, 1518, 1613, 2343 |
| CviQI | GTAC | 4 cut(s) | 641, 1518, 1613, 2343 |
| DdeI | CTNAG | 5 cut(s) | 207, 374, 917, 1065, 1325 |
| DpnI | GATC | 5 cut(s) | 461, 618, 1785, 2118, 2193 |
| DpnII | GATC | 5 cut(s) | 459, 616, 1783, 2116, 2191 |
| DraI | TTTAAA | 1 cut(s) | 1998 |
| DraIII | CACNNNGTG | 2 cut(s) | 2537, 2576 |
| EaeI | YGGCCR | 1 cut(s) | 1354 |
| Eam1104I | CTCTTC | 2 cut(s) | 45, 79 |
| EarI | CTCTTC | 2 cut(s) | 45, 79 |
| EciI | GGCGGA | 1 cut(s) | 169 |
| Eco130I | CCWWGG | 2 cut(s) | 60, 2031 |
| Eco147I | AGGCCT | 1 cut(s) | 9 |
| Eco24I | GRGCYC | 1 cut(s) | 2067 |
| Eco47I | GGWCC | 3 cut(s) | 399, 646, 2393 |
| Eco57I | CTGAAG | 3 cut(s) | 1052, 1800, 2722 |
| Eco88I | CYCGRG | 1 cut(s) | 626 |
| EcoNI | CCTNNNNNAGG | 3 cut(s) | 59, 194, 1853 |
| EcoRII | CCWGG | 4 cut(s) | 195, 2081, 2151, 2395 |
| EcoT14I | CCWWGG | 2 cut(s) | 60, 2031 |
| EcoT38I | GRGCYC | 1 cut(s) | 2067 |
| ErhI | CCWWGG | 2 cut(s) | 60, 2031 |
| FalI | AAGNNNNNCTT | 6 cut(s) | 420, 452, 489, 521, 1413, 1445 |
| FaqI | GGGAC | 2 cut(s) | 412, 722 |
| FauI | CCCGC | 1 cut(s) | 449 |
| FauNDI | CATATG | 1 cut(s) | 259 |
| FriOI | GRGCYC | 1 cut(s) | 2067 |
| FspBI | CTAG | 8 cut(s) | 32, 582, 1208, 1344, 1805, 2141, 2535, 2718 |
| GlaI | GCGC | 4 cut(s) | 139, 363, 388, 1602 |
| GsaI | CCCAGC | 2 cut(s) | 2115, 2214 |
| GsuI | CTGGAG | 2 cut(s) | 1208, 2547 |
| HaeII | RGCGCY | 2 cut(s) | 365, 390 |
| HaeIII | GGCC | 5 cut(s) | 9, 423, 1224, 1356, 2188 |
| HapII | CCGG | 2 cut(s) | 390, 644 |
| HhaI | GCGC | 4 cut(s) | 140, 364, 389, 1603 |
| Hin6I | GCGC | 4 cut(s) | 138, 362, 387, 1601 |
| HinP1I | GCGC | 4 cut(s) | 138, 362, 387, 1601 |
| HincII | GTYRAC | 3 cut(s) | 343, 1176, 2079 |
| HindII | GTYRAC | 3 cut(s) | 343, 1176, 2079 |
| HpaI | GTTAAC | 1 cut(s) | 1176 |
| HpaII | CCGG | 2 cut(s) | 390, 644 |
| HphI | GGTGA | 5 cut(s) | 247, 1414, 2267, 2562, 2647 |
| Hpy166II | GTNNAC | 6 cut(s) | 343, 864, 1176, 1405, 1798, 2079 |
| Hpy188III | TCNNGA | 7 cut(s) | 463, 506, 582, 605, 785, 2132, 2600 |
| Hpy8I | GTNNAC | 6 cut(s) | 343, 864, 1176, 1405, 1798, 2079 |
| Hpy99I | CGWCG | 3 cut(s) | 53, 95, 98 |
| HpyCH4III | ACNGT | 7 cut(s) | 693, 853, 1501, 1692, 1802, 1888, 2835 |
| HpyCH4IV | ACGT | 3 cut(s) | 441, 1267, 2611 |
| HpyF3I | CTNAG | 5 cut(s) | 207, 374, 917, 1065, 1325 |
| HpySE526I | ACGT | 3 cut(s) | 441, 1267, 2611 |
| HspAI | GCGC | 4 cut(s) | 138, 362, 387, 1601 |
| KpnI | GGTACC | 1 cut(s) | 644 |
| KspAI | GTTAAC | 1 cut(s) | 1176 |
| Kzo9I | GATC | 5 cut(s) | 459, 616, 1783, 2116, 2191 |
| LguI | GCTCTTC | 1 cut(s) | 45 |
| LmnI | GCTCC | 5 cut(s) | 333, 1183, 2566, 2747, 2754 |
| MabI | ACCWGGT | 1 cut(s) | 2395 |
| MaeI | CTAG | 8 cut(s) | 32, 582, 1208, 1344, 1805, 2141, 2535, 2718 |
| MaeII | ACGT | 3 cut(s) | 441, 1267, 2611 |
| MaeIII | GTNAC | 8 cut(s) | 169, 831, 1093, 1564, 1594, 1939, 2170, 2537 |
| MalI | GATC | 5 cut(s) | 461, 618, 1785, 2118, 2193 |
| MbiI | CCGCTC | 2 cut(s) | 38, 107 |
| MboI | GATC | 5 cut(s) | 459, 616, 1783, 2116, 2191 |
| MflI | RGATCY | 3 cut(s) | 1783, 2116, 2191 |
| MhlI | GDGCHC | 2 cut(s) | 268, 2067 |
| MlsI | TGGCCA | 1 cut(s) | 1356 |
| MluNI | TGGCCA | 1 cut(s) | 1356 |
| MlyI | GAGTC | 4 cut(s) | 95, 105, 538, 2383 |
| MmeI | TCCRAC | 5 cut(s) | 243, 616, 940, 961, 2445 |
| Mox20I | TGGCCA | 1 cut(s) | 1356 |
| MscI | TGGCCA | 1 cut(s) | 1356 |
| MslI | CAYNNNNRTG | 1 cut(s) | 1230 |
| Msp20I | TGGCCA | 1 cut(s) | 1356 |
| MspCI | CTTAAG | 1 cut(s) | 1513 |
| MspI | CCGG | 2 cut(s) | 390, 644 |
| MspR9I | CCNGG | 4 cut(s) | 197, 2083, 2153, 2397 |
| MvaI | CCWGG | 4 cut(s) | 197, 2083, 2153, 2397 |
| MvnI | CGCG | 2 cut(s) | 138, 830 |
| NdeI | CATATG | 1 cut(s) | 259 |
| NdeII | GATC | 5 cut(s) | 459, 616, 1783, 2116, 2191 |
| NlaIV | GGNNCC | 5 cut(s) | 400, 642, 802, 1758, 2562 |
| NmuCI | GTSAC | 3 cut(s) | 169, 1939, 2537 |
| NspI | RCATGY | 3 cut(s) | 1248, 1723, 2714 |
| PasI | CCCWGGG | 1 cut(s) | 196 |
| PceI | AGGCCT | 1 cut(s) | 9 |
| PciSI | GCTCTTC | 1 cut(s) | 45 |
| PflFI | GACNNNGTC | 1 cut(s) | 2585 |
| PleI | GAGTC | 4 cut(s) | 95, 105, 537, 2382 |
| PpsI | GAGTC | 4 cut(s) | 95, 105, 537, 2382 |
| PsiI | TTATAA | 2 cut(s) | 674, 2681 |
| Psp1406I | AACGTT | 1 cut(s) | 441 |
| Psp6I | CCWGG | 4 cut(s) | 195, 2081, 2151, 2395 |
| PspFI | CCCAGC | 2 cut(s) | 2111, 2210 |
| PspGI | CCWGG | 4 cut(s) | 195, 2081, 2151, 2395 |
| PspN4I | GGNNCC | 5 cut(s) | 400, 642, 802, 1758, 2562 |
| PspPI | GGNCC | 4 cut(s) | 399, 646, 1222, 2393 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 1709, 1741 |
| PstI | CTGCAG | 2 cut(s) | 1825, 2336 |
| PstNI | CAGNNNCTG | 2 cut(s) | 2174, 2351 |
| PsuI | RGATCY | 3 cut(s) | 1783, 2116, 2191 |
| PsyI | GACNNNGTC | 1 cut(s) | 2585 |
| RsaI | GTAC | 4 cut(s) | 642, 1519, 1614, 2344 |
| RsaNI | GTAC | 4 cut(s) | 641, 1518, 1613, 2343 |
| RseI | CAYNNNNRTG | 1 cut(s) | 1230 |
| SapI | GCTCTTC | 1 cut(s) | 45 |
| Sau3AI | GATC | 5 cut(s) | 459, 616, 1783, 2116, 2191 |
| Sau96I | GGNCC | 4 cut(s) | 399, 646, 1222, 2393 |
| SchI | GAGTC | 4 cut(s) | 95, 105, 538, 2383 |
| ScrFI | CCNGG | 4 cut(s) | 197, 2083, 2153, 2397 |
| SduI | GDGCHC | 2 cut(s) | 268, 2067 |
| SexAI | ACCWGGT | 1 cut(s) | 2395 |
| SfcI | CTRYAG | 3 cut(s) | 1821, 2332, 2412 |
| SinI | GGWCC | 3 cut(s) | 399, 646, 2393 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 1230 |
| SmlI | CTYRAG | 4 cut(s) | 506, 526, 1513, 2442 |
| SmoI | CTYRAG | 4 cut(s) | 506, 526, 1513, 2442 |
| SseBI | AGGCCT | 1 cut(s) | 9 |
| SspI | AATATT | 1 cut(s) | 1588 |
| SspMI | CTAG | 8 cut(s) | 32, 582, 1208, 1344, 1805, 2141, 2535, 2718 |
| StuI | AGGCCT | 1 cut(s) | 9 |
| StyD4I | CCNGG | 4 cut(s) | 195, 2081, 2151, 2395 |
| StyI | CCWWGG | 2 cut(s) | 60, 2031 |
| TaaI | ACNGT | 7 cut(s) | 693, 853, 1501, 1692, 1802, 1888, 2835 |
| TaiI | ACGT | 3 cut(s) | 444, 1270, 2614 |
| TaqI | TCGA | 4 cut(s) | 109, 462, 752, 2722 |
| TatI | WGTACW | 1 cut(s) | 2342 |
| TauI | GCSGC | 2 cut(s) | 38, 119 |
| TscAI | CASTG | 7 cut(s) | 871, 1170, 1333, 1884, 2596, 2673, 2784 |
| TseFI | GTSAC | 3 cut(s) | 169, 1939, 2537 |
| Tsp45I | GTSAC | 3 cut(s) | 169, 1939, 2537 |
| TspGWI | ACGGA | 3 cut(s) | 37, 2406, 2585 |
| TspRI | CASTG | 7 cut(s) | 871, 1170, 1333, 1884, 2596, 2673, 2784 |
| Tth111I | GACNNNGTC | 1 cut(s) | 2585 |
| Vha464I | CTTAAG | 1 cut(s) | 1513 |
| VpaK11BI | GGWCC | 3 cut(s) | 399, 646, 2393 |
| XagI | CCTNNNNNAGG | 3 cut(s) | 59, 194, 1853 |
| XapI | RAATTY | 4 cut(s) | 968, 1289, 1508, 2486 |
| XbaI | TCTAGA | 1 cut(s) | 581 |
| XceI | RCATGY | 3 cut(s) | 1248, 1723, 2714 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 326 |
| XspI | CTAG | 8 cut(s) | 32, 582, 1208, 1344, 1805, 2141, 2535, 2718 |