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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccI | GTMKAC | 1 cut(s) | 228 |
| AclWI | GGATC | 3 cut(s) | 24, 37, 195 |
| AcsI | RAATTY | 2 cut(s) | 148, 176 |
| AgsI | TTSAA | 1 cut(s) | 182 |
| AlwI | GGATC | 3 cut(s) | 24, 37, 195 |
| ApoI | RAATTY | 2 cut(s) | 148, 176 |
| AseI | ATTAAT | 1 cut(s) | 125 |
| BamHI | GGATCC | 1 cut(s) | 29 |
| BccI | CCATC | 1 cut(s) | 83 |
| BfaI | CTAG | 1 cut(s) | 201 |
| BmiI | GGNNCC | 1 cut(s) | 31 |
| BmsI | GCATC | 1 cut(s) | 128 |
| Bsa29I | ATCGAT | 1 cut(s) | 190 |
| Bse1I | ACTGG | 1 cut(s) | 124 |
| BseCI | ATCGAT | 1 cut(s) | 190 |
| BseGI | GGATG | 2 cut(s) | 94, 143 |
| BseMII | CTCAG | 1 cut(s) | 85 |
| BseNI | ACTGG | 1 cut(s) | 124 |
| BshVI | ATCGAT | 1 cut(s) | 190 |
| Bsp143I | GATC | 3 cut(s) | 22, 29, 187 |
| BspCNI | CTCAG | 1 cut(s) | 84 |
| BspDI | ATCGAT | 1 cut(s) | 190 |
| BspLI | GGNNCC | 1 cut(s) | 31 |
| BspPI | GGATC | 3 cut(s) | 24, 37, 195 |
| BsrI | ACTGG | 1 cut(s) | 124 |
| BssMI | GATC | 3 cut(s) | 22, 29, 187 |
| BssNAI | GTATAC | 1 cut(s) | 229 |
| Bst1107I | GTATAC | 1 cut(s) | 229 |
| BstDEI | CTNAG | 1 cut(s) | 71 |
| BstF5I | GGATG | 2 cut(s) | 94, 143 |
| BstKTI | GATC | 3 cut(s) | 25, 32, 190 |
| BstMBI | GATC | 3 cut(s) | 22, 29, 187 |
| BstX2I | RGATCY | 1 cut(s) | 29 |
| BstYI | RGATCY | 1 cut(s) | 29 |
| BstZ17I | GTATAC | 1 cut(s) | 229 |
| Bsu15I | ATCGAT | 1 cut(s) | 190 |
| BsuTUI | ATCGAT | 1 cut(s) | 190 |
| BtsCI | GGATG | 2 cut(s) | 94, 143 |
| ClaI | ATCGAT | 1 cut(s) | 190 |
| CviAII | CATG | 3 cut(s) | 7, 168, 208 |
| CviJI | RGCY | 2 cut(s) | 75, 200 |
| CviKI_1 | RGCY | 2 cut(s) | 75, 200 |
| DdeI | CTNAG | 1 cut(s) | 71 |
| DpnI | GATC | 3 cut(s) | 24, 31, 189 |
| DpnII | GATC | 3 cut(s) | 22, 29, 187 |
| DraI | TTTAAA | 1 cut(s) | 175 |
| EcoRI | GAATTC | 1 cut(s) | 148 |
| FaeI | CATG | 3 cut(s) | 10, 171, 211 |
| FaiI | YATR | 6 cut(s) | 8, 169, 195, 209, 222, 229 |
| FatI | CATG | 3 cut(s) | 6, 167, 207 |
| FblI | GTMKAC | 1 cut(s) | 228 |
| FokI | GGATG | 2 cut(s) | 101, 150 |
| FspBI | CTAG | 1 cut(s) | 201 |
| Hin1II | CATG | 3 cut(s) | 10, 171, 211 |
| HinfI | GANTC | 1 cut(s) | 52 |
| Hpy166II | GTNNAC | 1 cut(s) | 229 |
| Hpy188I | TCNGA | 1 cut(s) | 137 |
| Hpy8I | GTNNAC | 1 cut(s) | 229 |
| HpyAV | CCTTC | 1 cut(s) | 205 |
| HpyF3I | CTNAG | 1 cut(s) | 71 |
| Hsp92II | CATG | 3 cut(s) | 10, 171, 211 |
| Kzo9I | GATC | 3 cut(s) | 22, 29, 187 |
| LmnI | GCTCC | 1 cut(s) | 204 |
| LpnPI | CCDG | 2 cut(s) | 11, 105 |
| LweI | GCATC | 1 cut(s) | 128 |
| MaeI | CTAG | 1 cut(s) | 201 |
| MalI | GATC | 3 cut(s) | 24, 31, 189 |
| MboI | GATC | 3 cut(s) | 22, 29, 187 |
| MboII | GAAGA | 1 cut(s) | 22 |
| MfeI | CAATTG | 1 cut(s) | 182 |
| MflI | RGATCY | 1 cut(s) | 29 |
| MluCI | AATT | 4 cut(s) | 148, 176, 182, 217 |
| MmeI | TCCRAC | 1 cut(s) | 115 |
| MseI | TTAA | 5 cut(s) | 113, 125, 164, 174, 216 |
| MunI | CAATTG | 1 cut(s) | 182 |
| NdeII | GATC | 3 cut(s) | 22, 29, 187 |
| NlaIII | CATG | 3 cut(s) | 10, 171, 211 |
| NlaIV | GGNNCC | 1 cut(s) | 31 |
| PfeI | GAWTC | 1 cut(s) | 52 |
| PshBI | ATTAAT | 1 cut(s) | 125 |
| PspN4I | GGNNCC | 1 cut(s) | 31 |
| PsuI | RGATCY | 1 cut(s) | 29 |
| SaqAI | TTAA | 5 cut(s) | 113, 125, 164, 174, 216 |
| Sau3AI | GATC | 3 cut(s) | 22, 29, 187 |
| SetI | ASST | 1 cut(s) | 216 |
| SfaNI | GCATC | 1 cut(s) | 128 |
| SgeI | CNNG | 8 cut(s) | 19, 38, 89, 97, 132, 180, 213, 220 |
| Sse9I | AATT | 4 cut(s) | 148, 176, 182, 217 |
| SspMI | CTAG | 1 cut(s) | 201 |
| TaqI | TCGA | 1 cut(s) | 190 |
| TasI | AATT | 4 cut(s) | 148, 176, 182, 217 |
| TfiI | GAWTC | 1 cut(s) | 52 |
| Tru1I | TTAA | 5 cut(s) | 113, 125, 164, 174, 216 |
| Tru9I | TTAA | 5 cut(s) | 113, 125, 164, 174, 216 |
| TspDTI | ATGAA | 5 cut(s) | 23, 44, 53, 94, 224 |
| VspI | ATTAAT | 1 cut(s) | 125 |
| XapI | RAATTY | 2 cut(s) | 148, 176 |
| XmiI | GTMKAC | 1 cut(s) | 228 |
| XspI | CTAG | 1 cut(s) | 201 |