Rh6BG397100

Domain of unknown function (DUF4218)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0001939
Physical Location & Seq
Reverse (-)
62516825 .. 62528743
11919 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG397100.1

Sequence Viewer

Length: 543 bp
ATGAGGTTTGATTATGATGGGAAAGATACGAGTTATGGTAGCAATACTTATTATATACTAGGACTGAACATGGATAGAAAATGGATCAAAAATCCGAACAAGTTGAGTGATGAGTACAAGTGCGGTGTTGAGTCATTTCTTGAAGTTGCAAGGAAGCATGTCAATGTTAGGGGCAGAACTAAATGTCCTTGCATCAAGTGCCTAAATCATGATCGTCAAACTATACCAGTTGTGAAGATACATTTGAGGAACTATGGGATATCTACAAGTTATGACATTTGGACATCTCATGGAGAAACAATAGGACAGCGATCACAGAATTTTTCTCCCCCAATAGTTGAAGAGATTGCTGGAGCCAATGATGTTATCCATGATATTTTGAATGATGCATTCCCACCCATTGACATGGATCACATGGGTCAAGATCTAGAGGAGGATGTGCTACAAGGAGGCAATGAAGATGTTATGGATGATCATGGGTCTCATGGGATGAGGAGTCAACACGGCATATTTGGTTTAGAAAAGGGAAAGATTAGAGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4216 DUF4218 Transposase_21

Protein Analysis

180

Amino Acids

20.46

Weight (kDa)

5.81

Isoelectric Point (pI)

33.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transpos_assoc PF13963 26 - 99 1e-18 Transposase-associated domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 123
AclWI GGATC 2 cut(s) 92, 417
AcsI RAATTY 1 cut(s) 319
AfaI GTAC 1 cut(s) 116
AgsI TTSAA 3 cut(s) 143, 341, 382
AloI GAACNNNNNNTCC 2 cut(s) 169, 201
Alw26I GTCTC 1 cut(s) 486
AlwI GGATC 2 cut(s) 92, 417
ApoI RAATTY 1 cut(s) 319
BccI CCATC 1 cut(s) 11
BceAI ACGGC 1 cut(s) 520
BclI TGATCA 1 cut(s) 472
BcoDI GTCTC 1 cut(s) 486
BfaI CTAG 2 cut(s) 59, 428
BglII AGATCT 1 cut(s) 424
BmiI GGNNCC 1 cut(s) 355
BmsI GCATC 2 cut(s) 201, 376
BpmI CTGGAG 1 cut(s) 372
BsaI GGTCTC 1 cut(s) 486
Bse1I ACTGG 1 cut(s) 227
Bse3DI GCAATG 1 cut(s) 460
BseGI GGATG 3 cut(s) 442, 475, 495
BseMI GCAATG 1 cut(s) 460
BseNI ACTGG 1 cut(s) 227
BseRI GAGGAG 2 cut(s) 446, 508
BsmAI GTCTC 1 cut(s) 486
BsmI GAATGC 1 cut(s) 389
Bso31I GGTCTC 1 cut(s) 486
Bsp143I GATC 6 cut(s) 84, 211, 311, 409, 424, 472
BspACI CCGC 1 cut(s) 123
BspHI TCATGA 1 cut(s) 208
BspLI GGNNCC 1 cut(s) 355
BspPI GGATC 2 cut(s) 92, 417
BspTNI GGTCTC 1 cut(s) 486
BsrDI GCAATG 1 cut(s) 460
BsrI ACTGG 1 cut(s) 227
BssMI GATC 6 cut(s) 84, 211, 311, 409, 424, 472
Bst6I CTCTTC 1 cut(s) 336
BstAPI GCANNNNNTGC 1 cut(s) 198
BstF5I GGATG 3 cut(s) 442, 475, 495
BstKTI GATC 6 cut(s) 87, 214, 314, 412, 427, 475
BstMAI GTCTC 1 cut(s) 486
BstMBI GATC 6 cut(s) 84, 211, 311, 409, 424, 472
BstMWI GCNNNNNNNGC 1 cut(s) 198
BstNSI RCATGY 1 cut(s) 161
BstX2I RGATCY 1 cut(s) 424
BstXI CCANNNNNNTGG 1 cut(s) 406
BstYI RGATCY 1 cut(s) 424
BtsCI GGATG 3 cut(s) 442, 475, 495
CciI TCATGA 1 cut(s) 208
Csp6I GTAC 1 cut(s) 115
CviAII CATG 9 cut(s) 70, 158, 209, 290, 371, 406, 415, 476, 485
CviJI RGCY 1 cut(s) 356
CviKI_1 RGCY 1 cut(s) 356
CviQI GTAC 1 cut(s) 115
DpnI GATC 6 cut(s) 86, 213, 313, 411, 426, 474
DpnII GATC 6 cut(s) 84, 211, 311, 409, 424, 472
Eam1104I CTCTTC 1 cut(s) 336
EarI CTCTTC 1 cut(s) 336
Eco31I GGTCTC 1 cut(s) 486
Eco32I GATATC 1 cut(s) 261
EcoRV GATATC 1 cut(s) 261
EcoT22I ATGCAT 1 cut(s) 391
FaeI CATG 9 cut(s) 73, 161, 212, 293, 374, 409, 418, 479, 488
FatI CATG 9 cut(s) 69, 157, 208, 289, 370, 405, 414, 475, 484
FbaI TGATCA 1 cut(s) 472
FokI GGATG 3 cut(s) 449, 482, 502
FspBI CTAG 2 cut(s) 59, 428
GsuI CTGGAG 1 cut(s) 372
Hin1II CATG 9 cut(s) 73, 161, 212, 293, 374, 409, 418, 479, 488
HincII GTYRAC 1 cut(s) 500
HindII GTYRAC 1 cut(s) 500
HinfI GANTC 2 cut(s) 131, 496
Hpy166II GTNNAC 1 cut(s) 500
Hpy188I TCNGA 1 cut(s) 96
Hpy188III TCNNGA 4 cut(s) 140, 209, 422, 428
Hpy8I GTNNAC 1 cut(s) 500
HpyCH4V TGCA 3 cut(s) 149, 192, 389
HpyF10VI GCNNNNNNNGC 1 cut(s) 198
Hsp92II CATG 9 cut(s) 73, 161, 212, 293, 374, 409, 418, 479, 488
Ksp22I TGATCA 1 cut(s) 472
Kzo9I GATC 6 cut(s) 84, 211, 311, 409, 424, 472
LmnI GCTCC 1 cut(s) 353
LpnPI CCDG 2 cut(s) 240, 336
LweI GCATC 2 cut(s) 201, 376
MaeI CTAG 2 cut(s) 59, 428
MalI GATC 6 cut(s) 86, 213, 313, 411, 426, 474
MboI GATC 6 cut(s) 84, 211, 311, 409, 424, 472
MboII GAAGA 3 cut(s) 247, 353, 470
MflI RGATCY 1 cut(s) 424
MluCI AATT 1 cut(s) 319
MlyI GAGTC 2 cut(s) 140, 505
MnlI CCTC 5 cut(s) 240, 424, 427, 443, 486
Mph1103I ATGCAT 1 cut(s) 391
MseI TTAA 1 cut(s) 541
MslI CAYNNNNRTG 2 cut(s) 162, 404
Mva1269I GAATGC 1 cut(s) 389
MwoI GCNNNNNNNGC 1 cut(s) 198
NdeII GATC 6 cut(s) 84, 211, 311, 409, 424, 472
NlaIII CATG 9 cut(s) 73, 161, 212, 293, 374, 409, 418, 479, 488
NlaIV GGNNCC 1 cut(s) 355
NsiI ATGCAT 1 cut(s) 391
NspI RCATGY 1 cut(s) 161
PagI TCATGA 1 cut(s) 208
PctI GAATGC 1 cut(s) 389
PleI GAGTC 2 cut(s) 139, 504
PpsI GAGTC 2 cut(s) 139, 504
PspN4I GGNNCC 1 cut(s) 355
PsuI RGATCY 1 cut(s) 424
RsaI GTAC 1 cut(s) 116
RsaNI GTAC 1 cut(s) 115
RseI CAYNNNNRTG 2 cut(s) 162, 404
SaqAI TTAA 1 cut(s) 541
Sau3AI GATC 6 cut(s) 84, 211, 311, 409, 424, 472
SchI GAGTC 2 cut(s) 140, 505
SetI ASST 1 cut(s) 8
SfaNI GCATC 2 cut(s) 201, 376
SmiMI CAYNNNNRTG 2 cut(s) 162, 404
Sse9I AATT 1 cut(s) 319
SsiI CCGC 1 cut(s) 123
SspMI CTAG 2 cut(s) 59, 428
TasI AATT 1 cut(s) 319
TatI WGTACW 1 cut(s) 114
Tru1I TTAA 1 cut(s) 541
Tru9I TTAA 1 cut(s) 541
TspDTI ATGAA 1 cut(s) 471
XapI RAATTY 1 cut(s) 319
XbaI TCTAGA 1 cut(s) 427
XceI RCATGY 1 cut(s) 161
XspI CTAG 2 cut(s) 59, 428
Zsp2I ATGCAT 1 cut(s) 391
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.