Rorug04G0357600

1-acyl-sn-glycerol-3-phosphate acyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0001488
Physical Location & Seq
Forward (+)
52957249 .. 53070502
113254 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0357600.1

Sequence Viewer

Length: 1290 bp
ATGGGGTCTACAGAAGGGAGGCGTAGTCATCATAATTTGGTGCCTCTTGCTGCATTGATCAGCAGAGAGCTGAAGAATGAGAAGATGGAAAAGCCTACTGTCAGATACGGGCATGCGGCTCAATCCAGGAAGGGGGAGGATTATTTCCTGATCAAGACTGATGGTCAGCGAGTGCCCGGAAATTCTTCATCAACATTTTCTGCATTTGCGATCTTTGACGGGCACAATGGAAACGCAGCAGCCATATATACAAGGGAGAACTTGTTAAATCATGTGTTGGGTGCTATTCCCAGGGGGCTAGGAAGGGAGGAGTGGCTCCAAGCCTTACCTCGGGCCTTGGTTGCAGGGTTTGTCAAGGCTGATAAGGATTTCCAAAGTAGAGGAGAAACTTCTGGAACAACAGCTACTGTTGTAATAGTTGATGGATGGACTGTGACAGTAGCATCAGTAGGAGACTCCCGTTGTATTTTAGATACTCAGGGTGGTGCAGTTTCCACTTTAACTGTTGATCATAGACTTGAAGAGAATGTAGAAGAGAGGGAGCGTGTTGCTGCAAGTGGTGGAGAAGTAGGGAGGTTAAGCATTGTTGGTGGTGCGGAGATTGGTCCTCTTCGTTGTTGGCCAGGAGGTTTATGCCTTTCTAGGTCGATTGGAGACATGGATGTTGGAGAGTTCATAGTTCCAATACCATATGTGAAACAAGTCAAGCTATCAAATAGAGGAGGGAGGCTCATAATTGCTTCTGATGGCATCTGGGATGCCCTATCCTCAGAAGAGGCAGCAAAATCTTGCCGTGGGTTGCCCGCTGAACTTGCTGCTAGACAAGTGGTGAAGGAGGCTTTAAGGTCAAGGGGTTTAAAGGATGATACAACTTGTATAGTTGTTGACATAATCCCTCCTGATAACTCGGCACAGCCTACAACTCCCCCAAAAAAGCAGAACAAGCTCAGGGCCCTGTTGTTCAGGAAGAAGTCACGTGATTCTGCACATAAACTATCCAAGAAGCTATCAGCTGTGGGTATCGTGGAAGAACTATTTGAAGAAGGCTCTGCCATGCTTGCTGAAAGGCTGGGCAATGATGAGTGCACGGCACAATCAACAACATCTGGGCTGTTTATGTGTGCTGTATGCCAAGTTGACCTTGCACCGAGTGAGGGCATATCTGTTCATGCTGGTTCCATCTTCTCCATGAGTTCAAAGCCTTGGCAAGGGCCTTTCCTCTGTTCTGATTGTCGTAATAAGAAGGATGCCATGGAAGGAAAACGCCCTAGCGGAGTAAGAGTAGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00561 ko00564 ko01100 ko01110 ko04072 map00561 map00564 map01100 map01110 map04072
Pfam Domains
Protein Families
Acyltransf_C Acyltransferase

Protein Analysis

429

Amino Acids

46.1

Weight (kDa)

8.47

Isoelectric Point (pI)

37.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 61 - 282 6.6e-36 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 40
AccI GTMKAC 1 cut(s) 8
AciI CCGC 4 cut(s) 116, 596, 804, 1272
AcoI YGGCCR 1 cut(s) 620
AcsI RAATTY 1 cut(s) 181
AcuI CTGAAG 1 cut(s) 92
AcvI CACGTG 1 cut(s) 977
AdeI CACNNNGTG 1 cut(s) 1151
AfiI CCNNNNNNNGG 4 cut(s) 132, 330, 1154, 1208
AgsI TTSAA 3 cut(s) 521, 1040, 1197
AhdI GACNNNNNGTC 1 cut(s) 162
AjnI CCWGG 3 cut(s) 125, 290, 622
AluBI AGCT 6 cut(s) 70, 404, 709, 946, 1006, 1013
AluI AGCT 6 cut(s) 70, 404, 709, 946, 1006, 1013
Alw21I GWGCWC 1 cut(s) 1088
Alw26I GTCTC 2 cut(s) 447, 648
Alw44I GTGCAC 1 cut(s) 1084
AlwNI CAGNNNCTG 1 cut(s) 407
Ama87I CYCGRG 1 cut(s) 330
AoxI GGCC 4 cut(s) 333, 620, 951, 1211
ApaI GGGCCC 1 cut(s) 955
ApaLI GTGCAC 1 cut(s) 1084
ApeKI GCWGC 6 cut(s) 50, 236, 239, 551, 779, 815
ApoI RAATTY 1 cut(s) 181
Asp700I GAANNNNTTC 1 cut(s) 184
AspS9I GGNCC 5 cut(s) 333, 605, 951, 952, 1211
AsuC2I CCSGG 1 cut(s) 177
AsuHPI GGTGA 1 cut(s) 841
AvaI CYCGRG 1 cut(s) 330
AvaII GGWCC 1 cut(s) 605
BaeGI GKGCMC 4 cut(s) 177, 225, 955, 1088
BalI TGGCCA 1 cut(s) 622
BanI GGYRCC 1 cut(s) 40
BanII GRGCYC 1 cut(s) 955
BbrPI CACGTG 1 cut(s) 977
Bbv12I GWGCWC 1 cut(s) 1088
BbvI GCAGC 6 cut(s) 37, 248, 251, 538, 791, 802
BccI CCATC 6 cut(s) 79, 155, 416, 420, 740, 1187
BceAI ACGGC 2 cut(s) 777, 1104
BciT130I CCWGG 3 cut(s) 127, 292, 624
BclI TGATCA 3 cut(s) 57, 150, 508
BcnI CCSGG 1 cut(s) 177
BcoDI GTCTC 2 cut(s) 447, 648
BfaI CTAG 4 cut(s) 299, 642, 819, 1269
BfmI CTRYAG 1 cut(s) 9
BisI GCNGC 7 cut(s) 51, 117, 237, 240, 552, 780, 816
BlsI GCNGC 7 cut(s) 52, 118, 238, 241, 553, 781, 817
Bme1390I CCNGG 4 cut(s) 127, 177, 292, 624
Bme18I GGWCC 1 cut(s) 605
BmeRI GACNNNNNGTC 1 cut(s) 162
BmeT110I CYCGRG 1 cut(s) 330
BmgT120I GGNCC 5 cut(s) 333, 605, 951, 952, 1211
BmiI GGNNCC 4 cut(s) 42, 317, 953, 1177
BmrFI CCNGG 4 cut(s) 127, 177, 292, 624
BmsI GCATC 4 cut(s) 452, 748, 759, 1237
BplI GAGNNNNNCTC 2 cut(s) 714, 746
Bpu10I CCTNAGC 1 cut(s) 947
BpuMI CCSGG 1 cut(s) 177
BsaAI YACGTR 1 cut(s) 977
BsaJI CCNNGG 7 cut(s) 290, 291, 329, 336, 793, 1202, 1251
BsaXI ACNNNNNCTCC 5 cut(s) 10, 40, 248, 278, 1266
Bsc4I CCNNNNNNNGG 4 cut(s) 132, 330, 1154, 1208
Bse3DI GCAATG 1 cut(s) 1081
BseBI CCWGG 3 cut(s) 127, 292, 624
BseDI CCNNGG 7 cut(s) 290, 291, 329, 336, 793, 1202, 1251
BseGI GGATG 5 cut(s) 431, 667, 763, 868, 1252
BseLI CCNNNNNNNGG 4 cut(s) 132, 330, 1154, 1208
BseMI GCAATG 1 cut(s) 1081
BseMII CTCAG 3 cut(s) 491, 783, 961
BseRI GAGGAG 3 cut(s) 323, 396, 735
BseSI GKGCMC 4 cut(s) 177, 225, 955, 1088
BseXI GCAGC 6 cut(s) 37, 248, 251, 538, 791, 802
BseYI CCCAGC 1 cut(s) 1069
BsgI GTGCAG 2 cut(s) 507, 969
BshFI GGCC 4 cut(s) 335, 622, 953, 1213
BshNI GGYRCC 1 cut(s) 40
BsiHKAI GWGCWC 1 cut(s) 1088
BsiHKCI CYCGRG 1 cut(s) 330
BsiSI CCGG 1 cut(s) 177
BslI CCNNNNNNNGG 4 cut(s) 132, 330, 1154, 1208
BsmAI GTCTC 2 cut(s) 447, 648
BsnI GGCC 4 cut(s) 335, 622, 953, 1213
BsoBI CYCGRG 1 cut(s) 330
Bsp120I GGGCCC 1 cut(s) 951
Bsp1286I GDGCHC 4 cut(s) 177, 225, 955, 1088
Bsp143I GATC 4 cut(s) 57, 150, 210, 508
Bsp19I CCATGG 1 cut(s) 1251
BspACI CCGC 4 cut(s) 116, 596, 804, 1272
BspANI GGCC 4 cut(s) 335, 622, 953, 1213
BspCNI CTCAG 3 cut(s) 490, 782, 960
BspLI GGNNCC 4 cut(s) 42, 317, 953, 1177
BspT107I GGYRCC 1 cut(s) 40
BsrDI GCAATG 1 cut(s) 1081
BssECI CCNNGG 7 cut(s) 290, 291, 329, 336, 793, 1202, 1251
BssMI GATC 4 cut(s) 57, 150, 210, 508
BssT1I CCWWGG 3 cut(s) 336, 1202, 1251
Bst2UI CCWGG 3 cut(s) 127, 292, 624
Bst4CI ACNGT 5 cut(s) 100, 409, 433, 439, 505
Bst6I CTCTTC 4 cut(s) 516, 528, 615, 768
BstBAI YACGTR 1 cut(s) 977
BstC8I GCNNGC 3 cut(s) 114, 804, 1059
BstDEI CTNAG 3 cut(s) 477, 769, 947
BstDSI CCRYGG 2 cut(s) 793, 1251
BstENI CCTNNNNNAGG 1 cut(s) 1206
BstF5I GGATG 5 cut(s) 431, 667, 763, 868, 1252
BstKTI GATC 4 cut(s) 60, 153, 213, 511
BstMAI GTCTC 2 cut(s) 447, 648
BstMBI GATC 4 cut(s) 57, 150, 210, 508
BstMWI GCNNNNNNNGC 4 cut(s) 341, 812, 943, 1058
BstNI CCWGG 3 cut(s) 127, 292, 624
BstNSI RCATGY 1 cut(s) 116
BstSCI CCNGG 4 cut(s) 125, 175, 290, 622
BstSFI CTRYAG 1 cut(s) 9
BstSLI GKGCMC 4 cut(s) 177, 225, 955, 1088
BstV1I GCAGC 6 cut(s) 37, 248, 251, 538, 791, 802
BsuRI GGCC 4 cut(s) 335, 622, 953, 1213
BtgI CCRYGG 2 cut(s) 793, 1251
BtsCI GGATG 5 cut(s) 431, 667, 763, 868, 1252
Cac8I GCNNGC 3 cut(s) 114, 804, 1059
CaiI CAGNNNCTG 1 cut(s) 407
Cfr13I GGNCC 5 cut(s) 333, 605, 951, 952, 1211
CviAII CATG 7 cut(s) 113, 272, 658, 1054, 1169, 1189, 1252
DdeI CTNAG 3 cut(s) 477, 769, 947
DpnI GATC 4 cut(s) 59, 152, 212, 510
DpnII GATC 4 cut(s) 57, 150, 210, 508
DraI TTTAAA 1 cut(s) 858
DraIII CACNNNGTG 1 cut(s) 1151
DriI GACNNNNNGTC 1 cut(s) 162
EaeI YGGCCR 1 cut(s) 620
Eam1104I CTCTTC 4 cut(s) 516, 528, 615, 768
Eam1105I GACNNNNNGTC 1 cut(s) 162
EarI CTCTTC 4 cut(s) 516, 528, 615, 768
Eco130I CCWWGG 3 cut(s) 336, 1202, 1251
Eco24I GRGCYC 1 cut(s) 955
Eco47I GGWCC 1 cut(s) 605
Eco57I CTGAAG 1 cut(s) 92
Eco72I CACGTG 1 cut(s) 977
Eco88I CYCGRG 1 cut(s) 330
EcoNI CCTNNNNNAGG 1 cut(s) 1206
EcoO109I RGGNCCY 3 cut(s) 951, 952, 1211
EcoRII CCWGG 3 cut(s) 125, 290, 622
EcoT14I CCWWGG 3 cut(s) 336, 1202, 1251
EcoT38I GRGCYC 1 cut(s) 955
ErhI CCWWGG 3 cut(s) 336, 1202, 1251
FaeI CATG 7 cut(s) 116, 275, 661, 1057, 1172, 1192, 1255
FalI AAGNNNNNCTT 2 cut(s) 1125, 1157
FatI CATG 7 cut(s) 112, 271, 657, 1053, 1168, 1188, 1251
FauI CCCGC 1 cut(s) 811
FauNDI CATATG 1 cut(s) 691
FbaI TGATCA 3 cut(s) 57, 150, 508
FblI GTMKAC 1 cut(s) 8
Fnu4HI GCNGC 7 cut(s) 51, 117, 237, 240, 552, 780, 816
FokI GGATG 5 cut(s) 438, 674, 770, 875, 1259
FriOI GRGCYC 1 cut(s) 955
Fsp4HI GCNGC 7 cut(s) 51, 117, 237, 240, 552, 780, 816
FspBI CTAG 4 cut(s) 299, 642, 819, 1269
GluI GCNGC 7 cut(s) 51, 117, 237, 240, 552, 780, 816
GsaI CCCAGC 1 cut(s) 1073
HaeIII GGCC 4 cut(s) 335, 622, 953, 1213
HapII CCGG 1 cut(s) 177
Hin1II CATG 7 cut(s) 116, 275, 661, 1057, 1172, 1192, 1255
HincII GTYRAC 2 cut(s) 886, 1138
HindII GTYRAC 2 cut(s) 886, 1138
HinfI GANTC 2 cut(s) 455, 980
HpaII CCGG 1 cut(s) 177
HphI GGTGA 1 cut(s) 841
Hpy166II GTNNAC 4 cut(s) 9, 886, 1086, 1138
Hpy188I TCNGA 4 cut(s) 104, 745, 772, 1228
Hpy188III TCNNGA 5 cut(s) 148, 154, 393, 899, 964
Hpy8I GTNNAC 4 cut(s) 9, 886, 1086, 1138
HpyAV CCTTC 7 cut(s) 8, 124, 297, 826, 1037, 1237, 1250
HpyCH4III ACNGT 5 cut(s) 100, 409, 433, 439, 505
HpyCH4IV ACGT 1 cut(s) 976
HpyCH4V TGCA 8 cut(s) 53, 203, 344, 488, 554, 986, 1086, 1145
HpyF10VI GCNNNNNNNGC 4 cut(s) 341, 812, 943, 1058
HpyF3I CTNAG 3 cut(s) 477, 769, 947
HpySE526I ACGT 1 cut(s) 976
Hsp92II CATG 7 cut(s) 116, 275, 661, 1057, 1172, 1192, 1255
Ksp22I TGATCA 3 cut(s) 57, 150, 508
Kzo9I GATC 4 cut(s) 57, 150, 210, 508
LmnI GCTCC 2 cut(s) 321, 541
Lsp1109I GCAGC 6 cut(s) 37, 248, 251, 538, 791, 802
LweI GCATC 4 cut(s) 452, 748, 759, 1237
MaeI CTAG 4 cut(s) 299, 642, 819, 1269
MaeII ACGT 1 cut(s) 976
MaeIII GTNAC 2 cut(s) 433, 972
MalI GATC 4 cut(s) 59, 152, 212, 510
MboI GATC 4 cut(s) 57, 150, 210, 508
MhlI GDGCHC 4 cut(s) 177, 225, 955, 1088
MlsI TGGCCA 1 cut(s) 622
MluCI AATT 3 cut(s) 34, 181, 735
MluNI TGGCCA 1 cut(s) 622
MlyI GAGTC 1 cut(s) 449
MmeI TCCRAC 1 cut(s) 646
Mox20I TGGCCA 1 cut(s) 622
MroXI GAANNNNTTC 1 cut(s) 184
MscI TGGCCA 1 cut(s) 622
MseI TTAA 5 cut(s) 266, 500, 578, 842, 857
Msp20I TGGCCA 1 cut(s) 622
MspA1I CMGCKG 2 cut(s) 806, 1013
MspI CCGG 1 cut(s) 177
MspR9I CCNGG 4 cut(s) 127, 177, 292, 624
MvaI CCWGG 3 cut(s) 127, 292, 624
MwoI GCNNNNNNNGC 4 cut(s) 341, 812, 943, 1058
NciI CCSGG 1 cut(s) 177
NcoI CCATGG 1 cut(s) 1251
NdeI CATATG 1 cut(s) 691
NdeII GATC 4 cut(s) 57, 150, 210, 508
NlaIII CATG 7 cut(s) 116, 275, 661, 1057, 1172, 1192, 1255
NlaIV GGNNCC 4 cut(s) 42, 317, 953, 1177
NmeAIII GCCGAG 1 cut(s) 887
NmuCI GTSAC 2 cut(s) 433, 972
NspI RCATGY 1 cut(s) 116
PaeI GCATGC 1 cut(s) 116
PasI CCCWGGG 1 cut(s) 291
PdmI GAANNNNTTC 1 cut(s) 184
PfeI GAWTC 1 cut(s) 980
PfoI TCCNGGA 1 cut(s) 125
PkrI GCNGC 7 cut(s) 52, 118, 238, 241, 553, 781, 817
PleI GAGTC 1 cut(s) 449
PmaCI CACGTG 1 cut(s) 977
PmlI CACGTG 1 cut(s) 977
PpsI GAGTC 1 cut(s) 449
Ppu21I YACGTR 1 cut(s) 977
Psp6I CCWGG 3 cut(s) 125, 290, 622
PspCI CACGTG 1 cut(s) 977
PspFI CCCAGC 1 cut(s) 1069
PspGI CCWGG 3 cut(s) 125, 290, 622
PspN4I GGNNCC 4 cut(s) 42, 317, 953, 1177
PspOMI GGGCCC 1 cut(s) 951
PspPI GGNCC 5 cut(s) 333, 605, 951, 952, 1211
PsrI GAACNNNNNNTAC 2 cut(s) 388, 420
PstNI CAGNNNCTG 1 cut(s) 407
PvuII CAGCTG 1 cut(s) 1013
SaqAI TTAA 5 cut(s) 266, 500, 578, 842, 857
SatI GCNGC 7 cut(s) 51, 117, 237, 240, 552, 780, 816
Sau3AI GATC 4 cut(s) 57, 150, 210, 508
Sau96I GGNCC 5 cut(s) 333, 605, 951, 952, 1211
SchI GAGTC 1 cut(s) 449
ScrFI CCNGG 4 cut(s) 127, 177, 292, 624
SduI GDGCHC 4 cut(s) 177, 225, 955, 1088
SfaNI GCATC 4 cut(s) 452, 748, 759, 1237
SfcI CTRYAG 1 cut(s) 9
SinI GGWCC 1 cut(s) 605
SphI GCATGC 1 cut(s) 116
Sse9I AATT 3 cut(s) 34, 181, 735
SsiI CCGC 4 cut(s) 116, 596, 804, 1272
SspMI CTAG 4 cut(s) 299, 642, 819, 1269
StyD4I CCNGG 4 cut(s) 125, 175, 290, 622
StyI CCWWGG 3 cut(s) 336, 1202, 1251
TaaI ACNGT 5 cut(s) 100, 409, 433, 439, 505
TaiI ACGT 1 cut(s) 979
TaqI TCGA 1 cut(s) 647
TasI AATT 3 cut(s) 34, 181, 735
TauI GCSGC 1 cut(s) 119
TfiI GAWTC 1 cut(s) 980
Tru1I TTAA 5 cut(s) 266, 500, 578, 842, 857
Tru9I TTAA 5 cut(s) 266, 500, 578, 842, 857
TseFI GTSAC 2 cut(s) 433, 972
TseI GCWGC 6 cut(s) 50, 236, 239, 551, 779, 815
Tsp45I GTSAC 2 cut(s) 433, 972
TspDTI ATGAA 3 cut(s) 177, 664, 1157
VneI GTGCAC 1 cut(s) 1084
VpaK11BI GGWCC 1 cut(s) 605
XagI CCTNNNNNAGG 1 cut(s) 1206
XapI RAATTY 1 cut(s) 181
XceI RCATGY 1 cut(s) 116
XmiI GTMKAC 1 cut(s) 8
XmnI GAANNNNTTC 1 cut(s) 184
XspI CTAG 4 cut(s) 299, 642, 819, 1269
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.