Rw2G033300
ERF Family

Belongs to the protein kinase superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0000736
Physical Location & Seq
Reverse (-)
54572738 .. 54575496
2759 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G033300.1

Sequence Viewer

Length: 1596 bp
ATGGGTTGCGATTCTAAAAGAAGGCGTAGAGAGGATATGGGTGACACAACGATTTCATCATCCAAGAAGCAAAAGCTGATGCCGATGACAGGTTTGGAGCAGACCCAAAAGCTTCTTTGTTGCGGGATCTTGCAGGAGCTAATTGATCACCGTCATGGCTCTGTGGCTGTTCGAATCCCTGATTCTCAGGAGGTTCGAAGGCCTATGGATCTGTCCACCGTCAAATCCAAGTTGGAGAGAGACAACTACTCCAGTATTGATGCCTTTGTGGCCGATGTCAAGCTTACCTTCCAAAACGCGTCATGGTATTACTCTCTTGGAGAAAAACAGCGTGCAATGGCCAAGAATCTTGGTAAGGTTTTTGAGGCTAAGTGGAAATTACGGTTTCCTGAAACTAGGTCTTCAACCTTGAAAGCTTCTTGTTGTGCTAGTTTGGATAGGTCTGTGTCTGCTATTAGTCCTGTGAGTCCATTAAGTCTTCAACAACTCAACAACAACAACAACAAAAGAGGGGCCAAGAGGTCATCCAGAAACCTTGACGAAGAGAAGGGTTCTGCTTCTGCTATCACTTGTGATAAGGAACACCGAATCAAGCGCGGCCTTGACGAAGAGAAGGGTTCTGCTTCTGCTATCACTTGTGCAGTGGATAAAGATAAGGAACACCGAATCAAGCGCGGCCTTGACGAAGAGAAGGGTTCTGCTTCTGCTATCACTTGTGCAGTACTGGATAAAGATAAGGAACACCGAATCAAGCGCGGCCTTGACCAAGAGAAGGGTTCTGCTTCTGCTATCACTTGTGCAGTGAATAAATATAAGGAGCTCCGAATCAAACGGCTAAGGGAACAGGCTCGTAAGGAAATTTTGAGGGTTGAAGAGGCTGCTCGATTGAAAGTCGAGAATCCTTTACAAGATCTTCAACAACTCAACTTGTTGTGCAGTGGTGGCATCAAACAGGATTCTTGTTACAGGGTGTATCGTTGGTTGACACTTGACAAGTTGGGCATCTATTTGAAGAGGGATGAACTTGAAGATGTTTATGAAGAGGATGAATTTCGCATTGGAGATTGGGAAGAAGATGGAGAACAGAGTCCACAAACAACGAAATATTACATCCACATATGGTGGCATATCATCATCATCAAAGATAATAAGAATTTGACTGGGACAGCAAGATATGCAAGCATGAATACTCATCTTGGCATTGAACAAAGCCGAAGGGATGATCTCGAGCTCGAGTCACTTGGATATGTGCTTATGTACTTCCTAAGAGGAAGTCTTCCATGGCAGGGATTGAAGGCTGGAACTAAAAAGCAGAAGTATGAGAAGATCAGTGAAAAGAAAGTGTCAACATCAATTGAGGCCTTATGCCGTGGTTATCCCACCGAATTTGCCTCATACTTCCATTACTGTAGATCTCTACGTTTTGAGGATAAACCAGATTATGCTTATCCGAAGAGATTGTTTCGTGACCTTTTTATTCGCGAAGGTTTTCAGTTTGATTATGTCTTTGATTGGACGATCTTGAAATATCAGCAGTCGCAGATTGCCACACCTCCTACTCGTGCTCCTGTAAGTGACACAGATATTACTATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04011 ko04392 map04011 map04392
Pfam Domains
Protein Families
Pkinase

Protein Analysis

531

Amino Acids

61.16

Weight (kDa)

9.01

Isoelectric Point (pI)

56.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Bromodomain PF00439 63 - 114 2e-10 Bromodomain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 5 cut(s) 299, 597, 675, 756, 1482
AciI CCGC 4 cut(s) 123, 597, 675, 756
AclWI GGATC 2 cut(s) 134, 216
AcoI YGGCCR 2 cut(s) 270, 339
AcsI RAATTY 4 cut(s) 858, 1049, 1153, 1385
AfaI GTAC 2 cut(s) 723, 1259
AfiI CCNNNNNNNGG 3 cut(s) 89, 772, 1286
AflIII ACRYGT 1 cut(s) 297
AjuI GAANNNNNNNTTGG 2 cut(s) 1041, 1073
AluBI AGCT 7 cut(s) 76, 112, 139, 283, 416, 820, 1231
AluI AGCT 7 cut(s) 76, 112, 139, 283, 416, 820, 1231
Alw21I GWGCWC 3 cut(s) 822, 1233, 1567
Alw26I GTCTC 1 cut(s) 234
AlwI GGATC 2 cut(s) 134, 216
Ama87I CYCGRG 2 cut(s) 1226, 1232
AoxI GGCC 8 cut(s) 200, 270, 339, 513, 598, 676, 757, 1359
ApeKI GCWGC 1 cut(s) 878
ApoI RAATTY 4 cut(s) 858, 1049, 1153, 1385
Asp700I GAANNNNTTC 1 cut(s) 1488
AspLEI GCGC 3 cut(s) 597, 675, 756
AspS9I GGNCC 1 cut(s) 513
AsuHPI GGTGA 2 cut(s) 53, 140
AsuII TTCGAA 2 cut(s) 172, 196
AvaI CYCGRG 2 cut(s) 1226, 1232
BalI TGGCCA 1 cut(s) 341
BanII GRGCYC 2 cut(s) 822, 1233
BauI CACGAG 1 cut(s) 1560
BbsI GAAGAC 3 cut(s) 393, 470, 1268
Bbv12I GWGCWC 3 cut(s) 822, 1233, 1567
BbvI GCAGC 1 cut(s) 865
BccI CCATC 1 cut(s) 1070
BceAI ACGGC 2 cut(s) 848, 1353
BclI TGATCA 1 cut(s) 145
BcoDI GTCTC 1 cut(s) 234
BfaI CTAG 2 cut(s) 396, 429
BfmI CTRYAG 1 cut(s) 1408
BglI GCCNNNNNGGC 1 cut(s) 269
BglII AGATCT 2 cut(s) 910, 1412
BisI GCNGC 4 cut(s) 598, 676, 757, 879
BlsI GCNGC 4 cut(s) 599, 677, 758, 880
BmcAI AGTACT 1 cut(s) 723
BmeT110I CYCGRG 2 cut(s) 1226, 1232
BmgT120I GGNCC 1 cut(s) 513
BmiI GGNNCC 1 cut(s) 514
BmrI ACTGGG 1 cut(s) 1170
BmsI GCATC 4 cut(s) 69, 250, 954, 1011
BmuI ACTGGG 1 cut(s) 1170
BpiI GAAGAC 3 cut(s) 393, 470, 1268
BpmI CTGGAG 1 cut(s) 235
Bpu10I CCTNAGC 1 cut(s) 836
Bpu14I TTCGAA 2 cut(s) 172, 196
BsaJI CCNNGG 2 cut(s) 1280, 1369
BsaXI ACNNNNNCTCC 4 cut(s) 233, 263, 1549, 1579
Bsc4I CCNNNNNNNGG 3 cut(s) 89, 772, 1286
Bse1I ACTGG 3 cut(s) 252, 729, 1165
Bse3DI GCAATG 1 cut(s) 342
BseDI CCNNGG 2 cut(s) 1280, 1369
BseGI GGATG 6 cut(s) 59, 524, 1024, 1051, 1110, 1225
BseLI CCNNNNNNNGG 3 cut(s) 89, 772, 1286
BseMI GCAATG 1 cut(s) 342
BseMII CTCAG 1 cut(s) 200
BseNI ACTGG 3 cut(s) 252, 729, 1165
BseXI GCAGC 1 cut(s) 865
BsgI GTGCAG 4 cut(s) 660, 738, 819, 955
Bsh1236I CGCG 5 cut(s) 299, 597, 675, 756, 1482
BshFI GGCC 8 cut(s) 202, 272, 341, 515, 600, 678, 759, 1361
BsiHKAI GWGCWC 3 cut(s) 822, 1233, 1567
BsiHKCI CYCGRG 2 cut(s) 1226, 1232
BslFI GGGAC 1 cut(s) 1177
BslI CCNNNNNNNGG 3 cut(s) 89, 772, 1286
BsmAI GTCTC 1 cut(s) 234
BsmFI GGGAC 1 cut(s) 1177
BsnI GGCC 8 cut(s) 202, 272, 341, 515, 600, 678, 759, 1361
BsoBI CYCGRG 2 cut(s) 1226, 1232
Bsp119I TTCGAA 2 cut(s) 172, 196
Bsp1286I GDGCHC 3 cut(s) 822, 1233, 1567
Bsp143I GATC 8 cut(s) 126, 145, 208, 910, 1222, 1326, 1412, 1518
Bsp19I CCATGG 1 cut(s) 1280
Bsp68I TCGCGA 1 cut(s) 1482
BspACI CCGC 4 cut(s) 123, 597, 675, 756
BspANI GGCC 8 cut(s) 202, 272, 341, 515, 600, 678, 759, 1361
BspCNI CTCAG 1 cut(s) 199
BspFNI CGCG 5 cut(s) 299, 597, 675, 756, 1482
BspLI GGNNCC 1 cut(s) 514
BspPI GGATC 2 cut(s) 134, 216
BspT104I TTCGAA 2 cut(s) 172, 196
BsrDI GCAATG 1 cut(s) 342
BsrI ACTGG 3 cut(s) 252, 729, 1165
BssECI CCNNGG 2 cut(s) 1280, 1369
BssMI GATC 8 cut(s) 126, 145, 208, 910, 1222, 1326, 1412, 1518
BssSI CACGAG 1 cut(s) 1560
BssT1I CCWWGG 1 cut(s) 1280
Bst2BI CACGAG 1 cut(s) 1560
Bst4CI ACNGT 4 cut(s) 152, 220, 384, 1409
Bst6I CTCTTC 7 cut(s) 537, 603, 681, 867, 1007, 1035, 1448
BstAPI GCANNNNNTGC 1 cut(s) 1175
BstBI TTCGAA 2 cut(s) 172, 196
BstC8I GCNNGC 2 cut(s) 333, 1180
BstDEI CTNAG 4 cut(s) 186, 369, 836, 1265
BstDSI CCRYGG 2 cut(s) 1280, 1369
BstF5I GGATG 6 cut(s) 59, 524, 1024, 1051, 1110, 1225
BstFNI CGCG 5 cut(s) 299, 597, 675, 756, 1482
BstHHI GCGC 3 cut(s) 597, 675, 756
BstKTI GATC 8 cut(s) 129, 148, 211, 913, 1225, 1329, 1415, 1521
BstMAI GTCTC 1 cut(s) 234
BstMBI GATC 8 cut(s) 126, 145, 208, 910, 1222, 1326, 1412, 1518
BstMWI GCNNNNNNNGC 3 cut(s) 269, 942, 1175
BstSFI CTRYAG 1 cut(s) 1408
BstUI CGCG 5 cut(s) 299, 597, 675, 756, 1482
BstV1I GCAGC 1 cut(s) 865
BstV2I GAAGAC 3 cut(s) 393, 470, 1268
BstX2I RGATCY 4 cut(s) 126, 208, 910, 1412
BstYI RGATCY 4 cut(s) 126, 208, 910, 1412
BsuRI GGCC 8 cut(s) 202, 272, 341, 515, 600, 678, 759, 1361
BtgI CCRYGG 2 cut(s) 1280, 1369
BtsCI GGATG 6 cut(s) 59, 524, 1024, 1051, 1110, 1225
BtsI GCAGTG 3 cut(s) 648, 807, 943
BtsIMutI CAGTG 4 cut(s) 648, 807, 943, 1336
BtuMI TCGCGA 1 cut(s) 1482
Cac8I GCNNGC 2 cut(s) 333, 1180
CfoI GCGC 3 cut(s) 597, 675, 756
Cfr13I GGNCC 1 cut(s) 513
CseI GACGC 1 cut(s) 288
Csp6I GTAC 2 cut(s) 722, 1258
CspCI CAANNNNNGTGG 2 cut(s) 1369, 1404
CviAII CATG 4 cut(s) 155, 303, 1183, 1281
CviQI GTAC 2 cut(s) 722, 1258
DdeI CTNAG 4 cut(s) 186, 369, 836, 1265
DpnI GATC 8 cut(s) 128, 147, 210, 912, 1224, 1328, 1414, 1520
DpnII GATC 8 cut(s) 126, 145, 208, 910, 1222, 1326, 1412, 1518
EaeI YGGCCR 2 cut(s) 270, 339
Eam1104I CTCTTC 7 cut(s) 537, 603, 681, 867, 1007, 1035, 1448
EarI CTCTTC 7 cut(s) 537, 603, 681, 867, 1007, 1035, 1448
Ecl136II GAGCTC 2 cut(s) 820, 1231
Eco130I CCWWGG 1 cut(s) 1280
Eco147I AGGCCT 2 cut(s) 202, 1361
Eco24I GRGCYC 2 cut(s) 822, 1233
Eco53kI GAGCTC 2 cut(s) 820, 1231
Eco88I CYCGRG 2 cut(s) 1226, 1232
EcoICRI GAGCTC 2 cut(s) 820, 1231
EcoT14I CCWWGG 1 cut(s) 1280
EcoT38I GRGCYC 2 cut(s) 822, 1233
ErhI CCWWGG 1 cut(s) 1280
FaeI CATG 4 cut(s) 158, 306, 1186, 1284
FalI AAGNNNNNCTT 2 cut(s) 272, 304
FaqI GGGAC 1 cut(s) 1177
FatI CATG 4 cut(s) 154, 302, 1182, 1280
FauI CCCGC 1 cut(s) 116
FauNDI CATATG 1 cut(s) 1118
FbaI TGATCA 1 cut(s) 145
Fnu4HI GCNGC 4 cut(s) 598, 676, 757, 879
FokI GGATG 6 cut(s) 46, 511, 1031, 1058, 1097, 1232
FriOI GRGCYC 2 cut(s) 822, 1233
Fsp4HI GCNGC 4 cut(s) 598, 676, 757, 879
FspBI CTAG 2 cut(s) 396, 429
GlaI GCGC 3 cut(s) 596, 674, 755
GluI GCNGC 4 cut(s) 598, 676, 757, 879
GsuI CTGGAG 1 cut(s) 235
HaeIII GGCC 8 cut(s) 202, 272, 341, 515, 600, 678, 759, 1361
HgaI GACGC 1 cut(s) 288
HhaI GCGC 3 cut(s) 597, 675, 756
Hin1II CATG 4 cut(s) 158, 306, 1186, 1284
Hin6I GCGC 3 cut(s) 595, 673, 754
HinP1I GCGC 3 cut(s) 595, 673, 754
HincII GTYRAC 2 cut(s) 984, 1347
HindII GTYRAC 2 cut(s) 984, 1347
HindIII AAGCTT 3 cut(s) 110, 281, 414
HphI GGTGA 2 cut(s) 53, 140
Hpy166II GTNNAC 4 cut(s) 216, 984, 1091, 1347
Hpy188I TCNGA 2 cut(s) 824, 1452
Hpy188III TCNNGA 8 cut(s) 188, 389, 528, 895, 1226, 1466, 1481, 1522
Hpy8I GTNNAC 4 cut(s) 216, 984, 1091, 1347
HpyCH4III ACNGT 4 cut(s) 152, 220, 384, 1409
HpyCH4IV ACGT 1 cut(s) 1420
HpyCH4V TGCA 7 cut(s) 133, 335, 641, 719, 800, 936, 1178
HpyF10VI GCNNNNNNNGC 3 cut(s) 269, 942, 1175
HpyF3I CTNAG 4 cut(s) 186, 369, 836, 1265
HpySE526I ACGT 1 cut(s) 1420
Hsp92II CATG 4 cut(s) 158, 306, 1186, 1284
HspAI GCGC 3 cut(s) 595, 673, 754
Ksp22I TGATCA 1 cut(s) 145
Kzo9I GATC 8 cut(s) 126, 145, 208, 910, 1222, 1326, 1412, 1518
LmnI GCTCC 5 cut(s) 97, 136, 817, 825, 1570
Lsp1109I GCAGC 1 cut(s) 865
LweI GCATC 4 cut(s) 69, 250, 954, 1011
MaeI CTAG 2 cut(s) 396, 429
MaeII ACGT 1 cut(s) 1420
MaeIII GTNAC 5 cut(s) 41, 962, 1236, 1466, 1574
MalI GATC 8 cut(s) 128, 147, 210, 912, 1224, 1328, 1414, 1520
MboI GATC 8 cut(s) 126, 145, 208, 910, 1222, 1326, 1412, 1518
MfeI CAATTG 1 cut(s) 1353
MflI RGATCY 4 cut(s) 126, 208, 910, 1412
MhlI GDGCHC 3 cut(s) 822, 1233, 1567
MlsI TGGCCA 1 cut(s) 341
MluCI AATT 7 cut(s) 141, 377, 858, 1049, 1153, 1353, 1385
MluI ACGCGT 1 cut(s) 297
MluNI TGGCCA 1 cut(s) 341
MlyI GAGTC 3 cut(s) 475, 1096, 1244
MmeI TCCRAC 1 cut(s) 213
Mox20I TGGCCA 1 cut(s) 341
MroXI GAANNNNTTC 1 cut(s) 1488
MscI TGGCCA 1 cut(s) 341
MseI TTAA 1 cut(s) 473
MslI CAYNNNNRTG 1 cut(s) 153
Msp20I TGGCCA 1 cut(s) 341
MunI CAATTG 1 cut(s) 1353
MvnI CGCG 5 cut(s) 299, 597, 675, 756, 1482
MwoI GCNNNNNNNGC 3 cut(s) 269, 942, 1175
NcoI CCATGG 1 cut(s) 1280
NdeI CATATG 1 cut(s) 1118
NdeII GATC 8 cut(s) 126, 145, 208, 910, 1222, 1326, 1412, 1518
NlaIII CATG 4 cut(s) 158, 306, 1186, 1284
NlaIV GGNNCC 1 cut(s) 514
NmuCI GTSAC 4 cut(s) 41, 1236, 1466, 1574
NruI TCGCGA 1 cut(s) 1482
NspV TTCGAA 2 cut(s) 172, 196
PaeR7I CTCGAG 2 cut(s) 1226, 1232
PceI AGGCCT 2 cut(s) 202, 1361
PdmI GAANNNNTTC 1 cut(s) 1488
PkrI GCNGC 4 cut(s) 599, 677, 758, 880
PleI GAGTC 3 cut(s) 474, 1095, 1243
PpsI GAGTC 3 cut(s) 474, 1095, 1243
Psp124BI GAGCTC 2 cut(s) 822, 1233
PspN4I GGNNCC 1 cut(s) 514
PspPI GGNCC 1 cut(s) 513
PspXI VCTCGAGB 1 cut(s) 1232
PsuI RGATCY 4 cut(s) 126, 208, 910, 1412
RruI TCGCGA 1 cut(s) 1482
RsaI GTAC 2 cut(s) 723, 1259
RsaNI GTAC 2 cut(s) 722, 1258
RseI CAYNNNNRTG 1 cut(s) 153
SacI GAGCTC 2 cut(s) 822, 1233
SaqAI TTAA 1 cut(s) 473
SatI GCNGC 4 cut(s) 598, 676, 757, 879
Sau3AI GATC 8 cut(s) 126, 145, 208, 910, 1222, 1326, 1412, 1518
Sau96I GGNCC 1 cut(s) 513
ScaI AGTACT 1 cut(s) 723
SchI GAGTC 3 cut(s) 475, 1096, 1244
SduI GDGCHC 3 cut(s) 822, 1233, 1567
SfaNI GCATC 4 cut(s) 69, 250, 954, 1011
SfcI CTRYAG 1 cut(s) 1408
Sfr274I CTCGAG 2 cut(s) 1226, 1232
SfuI TTCGAA 2 cut(s) 172, 196
SlaI CTCGAG 2 cut(s) 1226, 1232
SmiMI CAYNNNNRTG 1 cut(s) 153
SmlI CTYRAG 2 cut(s) 1226, 1232
SmoI CTYRAG 2 cut(s) 1226, 1232
Sse9I AATT 7 cut(s) 141, 377, 858, 1049, 1153, 1353, 1385
SseBI AGGCCT 2 cut(s) 202, 1361
SsiI CCGC 4 cut(s) 123, 597, 675, 756
SspI AATATT 1 cut(s) 1106
SspMI CTAG 2 cut(s) 396, 429
SstI GAGCTC 2 cut(s) 822, 1233
StuI AGGCCT 2 cut(s) 202, 1361
StyI CCWWGG 1 cut(s) 1280
TaaI ACNGT 4 cut(s) 152, 220, 384, 1409
TaiI ACGT 1 cut(s) 1423
TaqI TCGA 6 cut(s) 172, 196, 883, 894, 1227, 1233
TasI AATT 7 cut(s) 141, 377, 858, 1049, 1153, 1353, 1385
TatI WGTACW 2 cut(s) 721, 1257
TauI GCSGC 3 cut(s) 600, 678, 759
Tru1I TTAA 1 cut(s) 473
Tru9I TTAA 1 cut(s) 473
TscAI CASTG 4 cut(s) 648, 807, 943, 1336
TseFI GTSAC 4 cut(s) 41, 1236, 1466, 1574
TseI GCWGC 1 cut(s) 878
Tsp45I GTSAC 4 cut(s) 41, 1236, 1466, 1574
TspDTI ATGAA 5 cut(s) 45, 1035, 1053, 1062, 1199
TspRI CASTG 4 cut(s) 648, 807, 943, 1336
XapI RAATTY 4 cut(s) 858, 1049, 1153, 1385
XhoI CTCGAG 2 cut(s) 1226, 1232
XmnI GAANNNNTTC 1 cut(s) 1488
XspI CTAG 2 cut(s) 396, 429
ZrmI AGTACT 1 cut(s) 723
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.