AT1G47960

cell wall vacuolar inhibitor of fructosidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0000921
Physical Location & Seq
Reverse (-)
17681157 .. 17683719
2563 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G47960.2

Sequence Viewer

Length: 618 bp
ATGAAGATGATGAAGGTGATGATGTTGATCGTGATGATGATGATGGTGATGGTGATGGTGAGTGAAGGAAGTATAATAGAGCCAACATGTAAAGAGACACCAGACTTCAATCTTTGTGTGTCTCTCCTTAACTCCGATCCACGTGGTTCCTCTGCCGACACTTCTGGCCTCGCTCTCATCCTCATTGATAAAATCAAGGGCCTGGCAACAAAAACTTTGAACGAGATCAACGGTCTATATAAGAAGAGACCAGAACTAAAACGGGCTTTGGATGAATGTAGTCGAAGATACAAAACGATCTTAAACGCTGATGTTCCCGAAGCCATTGAAGCTATCTCTAAAGGAGTCCCCAAATTCGGCGAAGACGGTGTGATCGACGCCGGTGTCGAAGCTTCTGTTTGTCAAGGAGGGTTTAATGGGAGTTCTCCGTTGACTAGTTTGACTAAATCAATGCAAAAAATCTCTAATGTGACTAGAGCCATTTTTTATAGTAATTCCATCGTCAAAGAAGAAGCTTGTGGATCTTCGTGGCCTTCTCTGGCGTTAAATATAGATTCGAAGGCTTGTGTTGTTTCATTGCAAAACATTCAATTCAATAGAGGAAGAACTTGTTGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0004857 GO:0005575 GO:0005618 GO:0005623 GO:0008150 GO:0030234 GO:0030312 GO:0043086 GO:0044092 GO:0044464 GO:0050790 GO:0065007 GO:0065009 GO:0071944 GO:0098772
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

205

Amino Acids

22.33

Weight (kDa)

8.44

Isoelectric Point (pI)

44.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 25 - 156 6.6e-15 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 383
AclWI GGATC 2 cut(s) 131, 529
AcsI RAATTY 1 cut(s) 353
AcvI CACGTG 1 cut(s) 143
AcyI GRCGYC 1 cut(s) 378
AfiI CCNNNNNNNGG 1 cut(s) 356
AflIII ACRYGT 1 cut(s) 86
AgsI TTSAA 5 cut(s) 109, 220, 329, 590, 595
AhlI ACTAGT 1 cut(s) 434
AjnI CCWGG 1 cut(s) 201
AjuI GAANNNNNNNTTGG 1 cut(s) 595
AluBI AGCT 3 cut(s) 332, 392, 515
AluI AGCT 3 cut(s) 332, 392, 515
Alw26I GTCTC 3 cut(s) 89, 126, 241
AlwI GGATC 2 cut(s) 131, 529
AoxI GGCC 3 cut(s) 166, 199, 530
ApoI RAATTY 1 cut(s) 353
AspS9I GGNCC 1 cut(s) 199
AsuHPI GGTGA 4 cut(s) 28, 58, 64, 70
AsuII TTCGAA 1 cut(s) 557
BbrPI CACGTG 1 cut(s) 143
BbsI GAAGAC 1 cut(s) 369
BccI CCATC 4 cut(s) 37, 43, 49, 506
BciT130I CCWGG 1 cut(s) 203
BcoDI GTCTC 3 cut(s) 89, 126, 241
BcuI ACTAGT 1 cut(s) 434
BfaI CTAG 2 cut(s) 435, 474
Bme1390I CCNGG 1 cut(s) 203
BmgT120I GGNCC 1 cut(s) 199
BmiI GGNNCC 1 cut(s) 148
BmrFI CCNGG 1 cut(s) 203
BpiI GAAGAC 1 cut(s) 369
Bpu14I TTCGAA 1 cut(s) 557
BsaAI YACGTR 1 cut(s) 143
BsaBI GATNNNNATC 1 cut(s) 26
BsaHI GRCGYC 1 cut(s) 378
BsaI GGTCTC 1 cut(s) 241
Bsc4I CCNNNNNNNGG 1 cut(s) 356
Bse118I RCCGGY 1 cut(s) 380
Bse3DI GCAATG 1 cut(s) 575
Bse8I GATNNNNATC 1 cut(s) 26
BseBI CCWGG 1 cut(s) 203
BseGI GGATG 2 cut(s) 177, 277
BseJI GATNNNNATC 1 cut(s) 26
BseLI CCNNNNNNNGG 1 cut(s) 356
BseMI GCAATG 1 cut(s) 575
BshFI GGCC 3 cut(s) 168, 201, 532
BsiSI CCGG 1 cut(s) 381
BslFI GGGAC 1 cut(s) 332
BslI CCNNNNNNNGG 1 cut(s) 356
BsmAI GTCTC 3 cut(s) 89, 126, 241
BsmFI GGGAC 1 cut(s) 332
BsnI GGCC 3 cut(s) 168, 201, 532
Bso31I GGTCTC 1 cut(s) 241
Bsp119I TTCGAA 1 cut(s) 557
Bsp143I GATC 6 cut(s) 27, 136, 225, 297, 372, 521
BspANI GGCC 3 cut(s) 168, 201, 532
BspLI GGNNCC 1 cut(s) 148
BspPI GGATC 2 cut(s) 131, 529
BspT104I TTCGAA 1 cut(s) 557
BspTNI GGTCTC 1 cut(s) 241
BsrDI GCAATG 1 cut(s) 575
BsrFI RCCGGY 1 cut(s) 380
BssAI RCCGGY 1 cut(s) 380
BssMI GATC 6 cut(s) 27, 136, 225, 297, 372, 521
BssNI GRCGYC 1 cut(s) 378
Bst2UI CCWGG 1 cut(s) 203
Bst4CI ACNGT 2 cut(s) 233, 368
Bst6I CTCTTC 1 cut(s) 239
BstACI GRCGYC 1 cut(s) 378
BstBAI YACGTR 1 cut(s) 143
BstBI TTCGAA 1 cut(s) 557
BstF5I GGATG 2 cut(s) 177, 277
BstKTI GATC 6 cut(s) 30, 139, 228, 300, 375, 524
BstMAI GTCTC 3 cut(s) 89, 126, 241
BstMBI GATC 6 cut(s) 27, 136, 225, 297, 372, 521
BstMWI GCNNNNNNNGC 1 cut(s) 329
BstNI CCWGG 1 cut(s) 203
BstNSI RCATGY 1 cut(s) 90
BstSCI CCNGG 1 cut(s) 201
BstV2I GAAGAC 1 cut(s) 369
BstX2I RGATCY 1 cut(s) 521
BstYI RGATCY 1 cut(s) 521
BsuRI GGCC 3 cut(s) 168, 201, 532
BtsCI GGATG 2 cut(s) 177, 277
Cfr10I RCCGGY 1 cut(s) 380
Cfr13I GGNCC 1 cut(s) 199
CseI GACGC 1 cut(s) 386
CviAII CATG 1 cut(s) 87
DpnI GATC 6 cut(s) 29, 138, 227, 299, 374, 523
DpnII GATC 6 cut(s) 27, 136, 225, 297, 372, 521
DrdI GACNNNNNNGTC 1 cut(s) 383
DseDI GACNNNNNNGTC 1 cut(s) 383
Eam1104I CTCTTC 1 cut(s) 239
EarI CTCTTC 1 cut(s) 239
Eco31I GGTCTC 1 cut(s) 241
Eco72I CACGTG 1 cut(s) 143
EcoO109I RGGNCCY 1 cut(s) 199
EcoRII CCWGG 1 cut(s) 201
FaeI CATG 1 cut(s) 90
FaiI YATR 6 cut(s) 74, 88, 238, 240, 489, 551
FaqI GGGAC 1 cut(s) 332
FatI CATG 1 cut(s) 86
FokI GGATG 2 cut(s) 164, 284
FspBI CTAG 2 cut(s) 435, 474
HaeIII GGCC 3 cut(s) 168, 201, 532
HapII CCGG 1 cut(s) 381
HgaI GACGC 1 cut(s) 386
Hin1I GRCGYC 1 cut(s) 378
Hin1II CATG 1 cut(s) 90
HincII GTYRAC 1 cut(s) 432
HindII GTYRAC 1 cut(s) 432
HindIII AAGCTT 2 cut(s) 390, 513
HinfI GANTC 2 cut(s) 345, 554
HpaII CCGG 1 cut(s) 381
HphI GGTGA 4 cut(s) 28, 58, 64, 70
Hpy166II GTNNAC 1 cut(s) 432
Hpy188I TCNGA 1 cut(s) 136
Hpy188III TCNNGA 2 cut(s) 31, 317
Hpy8I GTNNAC 1 cut(s) 432
Hpy99I CGWCG 1 cut(s) 380
HpyAV CCTTC 4 cut(s) 7, 59, 543, 553
HpyCH4III ACNGT 2 cut(s) 233, 368
HpyCH4IV ACGT 1 cut(s) 142
HpyCH4V TGCA 2 cut(s) 454, 580
HpyF10VI GCNNNNNNNGC 1 cut(s) 329
HpySE526I ACGT 1 cut(s) 142
Hsp92I GRCGYC 1 cut(s) 378
Hsp92II CATG 1 cut(s) 90
Kzo9I GATC 6 cut(s) 27, 136, 225, 297, 372, 521
LpnPI CCDG 7 cut(s) 114, 150, 188, 215, 264, 394, 524
MaeI CTAG 2 cut(s) 435, 474
MaeII ACGT 1 cut(s) 142
MaeIII GTNAC 1 cut(s) 469
MalI GATC 6 cut(s) 29, 138, 227, 299, 374, 523
MboI GATC 6 cut(s) 27, 136, 225, 297, 372, 521
MboII GAAGA 7 cut(s) 16, 256, 297, 374, 516, 521, 615
MflI RGATCY 1 cut(s) 521
MluCI AATT 3 cut(s) 353, 493, 590
MlyI GAGTC 1 cut(s) 354
MnlI CCTC 5 cut(s) 160, 179, 191, 401, 593
MseI TTAA 4 cut(s) 129, 302, 414, 545
MspI CCGG 1 cut(s) 381
MspR9I CCNGG 1 cut(s) 203
MvaI CCWGG 1 cut(s) 203
MwoI GCNNNNNNNGC 1 cut(s) 329
NdeII GATC 6 cut(s) 27, 136, 225, 297, 372, 521
NlaIII CATG 1 cut(s) 90
NlaIV GGNNCC 1 cut(s) 148
NmuCI GTSAC 1 cut(s) 469
NspI RCATGY 1 cut(s) 90
NspV TTCGAA 1 cut(s) 557
PciI ACATGT 1 cut(s) 86
PcsI WCGNNNNNNNCGW 2 cut(s) 372, 384
PfeI GAWTC 1 cut(s) 554
PleI GAGTC 1 cut(s) 353
PmaCI CACGTG 1 cut(s) 143
PmlI CACGTG 1 cut(s) 143
PpsI GAGTC 1 cut(s) 353
Ppu21I YACGTR 1 cut(s) 143
PscI ACATGT 1 cut(s) 86
Psp6I CCWGG 1 cut(s) 201
PspCI CACGTG 1 cut(s) 143
PspGI CCWGG 1 cut(s) 201
PspN4I GGNNCC 1 cut(s) 148
PspPI GGNCC 1 cut(s) 199
PsuI RGATCY 1 cut(s) 521
SaqAI TTAA 4 cut(s) 129, 302, 414, 545
Sau3AI GATC 6 cut(s) 27, 136, 225, 297, 372, 521
Sau96I GGNCC 1 cut(s) 199
SchI GAGTC 1 cut(s) 354
ScrFI CCNGG 1 cut(s) 203
SetI ASST 5 cut(s) 18, 145, 334, 394, 517
SfuI TTCGAA 1 cut(s) 557
SgrAI CRCCGGYG 1 cut(s) 380
SpeI ACTAGT 1 cut(s) 434
Sse9I AATT 3 cut(s) 353, 493, 590
SspMI CTAG 2 cut(s) 435, 474
StyD4I CCNGG 1 cut(s) 201
TaaI ACNGT 2 cut(s) 233, 368
TaiI ACGT 1 cut(s) 145
TaqI TCGA 4 cut(s) 283, 375, 387, 557
TasI AATT 3 cut(s) 353, 493, 590
TfiI GAWTC 1 cut(s) 554
Tru1I TTAA 4 cut(s) 129, 302, 414, 545
Tru9I TTAA 4 cut(s) 129, 302, 414, 545
TseFI GTSAC 1 cut(s) 469
Tsp45I GTSAC 1 cut(s) 469
TspDTI ATGAA 4 cut(s) 17, 26, 288, 564
TspGWI ACGGA 1 cut(s) 417
XapI RAATTY 1 cut(s) 353
XceI RCATGY 1 cut(s) 90
XspI CTAG 2 cut(s) 435, 474
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.