Prupe.1G114500_v2.0.a1

Cell wall vacuolar inhibitor of fructosidase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0000921
Physical Location & Seq
Reverse (-)
9074127 .. 9074847
721 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G114500.1

Sequence Viewer

Length: 531 bp
ATGAACAACTCAACATCTCTCCTTCAACTTGTTTGTTGTACCCTACTTGTCTTTCTTCTTCCAGTGGCCCTCTGCAGCAGGGTTGTCTTCCCAACAGACGCCAATCTCATCGACCAAACCTGCAAACAGACACCGAACTATGCTCTCTGCGTCTCTTCTCTTAAATCGGACCCTCGGAGCTCCACCGCAGACGTATCGGGCCTAGGCGTAATACTGGTGGATATAGTGAAGGCCAAGTCGACCGACACTCTGAACAAAATCAACACTCTGCTTAAGCAGAGACCTGGAGACCAGGCCTTGAAATCTTGCGCTCGGTTTTACGACACGGTTGTAGAAGCTTTGGTGCCTGAAGCGCAACAGGGTTTTAGCTTAGGCAACCCCAAGTTTGCAGAGCAAGGCATGAACGACGCTGCCGGTGAGGCCAAGACGTGTGAAGATGGTTTCTCTGGCAAGTCTCCTCTGACGGATAACAACAAGGCTGTGCATGATATTGCGGCTGTGGCTGCAGCAATTGCCAGGACATTGCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

177

Amino Acids

18.63

Weight (kDa)

5.78

Isoelectric Point (pI)

36.02

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 128
AccB1I GGYRCC 1 cut(s) 343
AccI GTMKAC 1 cut(s) 239
AciI CCGC 2 cut(s) 186, 494
AcuI CTGAAG 1 cut(s) 369
AcyI GRCGYC 1 cut(s) 99
AfaI GTAC 1 cut(s) 40
AflII CTTAAG 1 cut(s) 272
AflIII ACRYGT 1 cut(s) 428
AgsI TTSAA 2 cut(s) 26, 301
AjiI CACGTC 1 cut(s) 429
AjnI CCWGG 3 cut(s) 283, 291, 515
AluBI AGCT 3 cut(s) 180, 338, 369
AluI AGCT 3 cut(s) 180, 338, 369
Alw21I GWGCWC 1 cut(s) 182
Alw26I GTCTC 4 cut(s) 157, 274, 282, 459
AoxI GGCC 5 cut(s) 66, 199, 231, 294, 420
ApeKI GCWGC 4 cut(s) 75, 410, 503, 506
AspA2I CCTAGG 1 cut(s) 202
AspLEI GCGC 2 cut(s) 311, 355
AspS9I GGNCC 3 cut(s) 67, 169, 199
AsuHPI GGTGA 1 cut(s) 428
AvaII GGWCC 1 cut(s) 169
AvrII CCTAGG 1 cut(s) 202
BanI GGYRCC 1 cut(s) 343
BanII GRGCYC 1 cut(s) 182
BbsI GAAGAC 1 cut(s) 79
Bbv12I GWGCWC 1 cut(s) 182
BbvI GCAGC 4 cut(s) 87, 397, 490, 518
BccI CCATC 1 cut(s) 431
BcgI CGANNNNNNTGC 2 cut(s) 177, 211
BciT130I CCWGG 3 cut(s) 285, 293, 517
BcoDI GTCTC 4 cut(s) 157, 274, 282, 459
BfaI CTAG 1 cut(s) 203
BfmI CTRYAG 2 cut(s) 73, 504
BfrI CTTAAG 1 cut(s) 272
BfuAI ACCTGC 1 cut(s) 128
BglI GCCNNNNNGGC 1 cut(s) 419
BisI GCNGC 5 cut(s) 76, 411, 495, 504, 507
BlnI CCTAGG 1 cut(s) 202
BlsI GCNGC 5 cut(s) 77, 412, 496, 505, 508
Bme1390I CCNGG 3 cut(s) 285, 293, 517
Bme18I GGWCC 1 cut(s) 169
BmgBI CACGTC 1 cut(s) 429
BmgT120I GGNCC 3 cut(s) 67, 169, 199
BmiI GGNNCC 2 cut(s) 171, 345
BmrFI CCNGG 3 cut(s) 285, 293, 517
BpiI GAAGAC 1 cut(s) 79
BpmI CTGGAG 1 cut(s) 306
Bpu10I CCTNAGC 1 cut(s) 370
BsaHI GRCGYC 1 cut(s) 99
BsaI GGTCTC 2 cut(s) 274, 282
BsaJI CCNNGG 2 cut(s) 173, 202
Bse118I RCCGGY 1 cut(s) 413
Bse1I ACTGG 2 cut(s) 62, 219
Bse3DI GCAATG 1 cut(s) 521
BseBI CCWGG 3 cut(s) 285, 293, 517
BseDI CCNNGG 2 cut(s) 173, 202
BseMI GCAATG 1 cut(s) 521
BseNI ACTGG 2 cut(s) 62, 219
BseRI GAGGAG 1 cut(s) 447
BseXI GCAGC 4 cut(s) 87, 397, 490, 518
Bsh1285I CGRYCG 1 cut(s) 243
BshFI GGCC 5 cut(s) 68, 201, 233, 296, 422
BshNI GGYRCC 1 cut(s) 343
BsiEI CGRYCG 1 cut(s) 243
BsiHKAI GWGCWC 1 cut(s) 182
BsiSI CCGG 1 cut(s) 414
BsmAI GTCTC 4 cut(s) 157, 274, 282, 459
BsmBI CGTCTC 1 cut(s) 157
BsnI GGCC 5 cut(s) 68, 201, 233, 296, 422
Bso31I GGTCTC 2 cut(s) 274, 282
Bsp1286I GDGCHC 1 cut(s) 182
BspACI CCGC 2 cut(s) 186, 494
BspANI GGCC 5 cut(s) 68, 201, 233, 296, 422
BspLI GGNNCC 2 cut(s) 171, 345
BspMAI CTGCAG 2 cut(s) 77, 508
BspMI ACCTGC 1 cut(s) 128
BspT107I GGYRCC 1 cut(s) 343
BspTI CTTAAG 1 cut(s) 272
BspTNI GGTCTC 2 cut(s) 274, 282
BsrDI GCAATG 1 cut(s) 521
BsrFI RCCGGY 1 cut(s) 413
BsrI ACTGG 2 cut(s) 62, 219
BssAI RCCGGY 1 cut(s) 413
BssECI CCNNGG 2 cut(s) 173, 202
BssNI GRCGYC 1 cut(s) 99
BssT1I CCWWGG 1 cut(s) 202
Bst2UI CCWGG 3 cut(s) 285, 293, 517
Bst4CI ACNGT 1 cut(s) 328
Bst6I CTCTTC 1 cut(s) 160
BstACI GRCGYC 1 cut(s) 99
BstAFI CTTAAG 1 cut(s) 272
BstAPI GCANNNNNTGC 1 cut(s) 512
BstDEI CTNAG 1 cut(s) 370
BstHHI GCGC 2 cut(s) 311, 355
BstMAI GTCTC 4 cut(s) 157, 274, 282, 459
BstMCI CGRYCG 1 cut(s) 243
BstMWI GCNNNNNNNGC 5 cut(s) 352, 419, 500, 503, 512
BstNI CCWGG 3 cut(s) 285, 293, 517
BstSCI CCNGG 3 cut(s) 283, 291, 515
BstSFI CTRYAG 2 cut(s) 73, 504
BstV1I GCAGC 4 cut(s) 87, 397, 490, 518
BstV2I GAAGAC 1 cut(s) 79
BsuRI GGCC 5 cut(s) 68, 201, 233, 296, 422
BtrI CACGTC 1 cut(s) 429
BtsIMutI CAGTG 1 cut(s) 69
BveI ACCTGC 1 cut(s) 128
CfoI GCGC 2 cut(s) 311, 355
Cfr10I RCCGGY 1 cut(s) 413
Cfr13I GGNCC 3 cut(s) 67, 169, 199
CseI GACGC 3 cut(s) 107, 139, 416
Csp6I GTAC 1 cut(s) 39
CviAII CATG 2 cut(s) 400, 485
CviQI GTAC 1 cut(s) 39
DdeI CTNAG 1 cut(s) 370
Eam1104I CTCTTC 1 cut(s) 160
EarI CTCTTC 1 cut(s) 160
Ecl136II GAGCTC 1 cut(s) 180
Eco130I CCWWGG 1 cut(s) 202
Eco147I AGGCCT 1 cut(s) 296
Eco24I GRGCYC 1 cut(s) 182
Eco31I GGTCTC 2 cut(s) 274, 282
Eco47I GGWCC 1 cut(s) 169
Eco53kI GAGCTC 1 cut(s) 180
Eco57I CTGAAG 1 cut(s) 369
EcoICRI GAGCTC 1 cut(s) 180
EcoRII CCWGG 3 cut(s) 283, 291, 515
EcoT14I CCWWGG 1 cut(s) 202
EcoT38I GRGCYC 1 cut(s) 182
ErhI CCWWGG 1 cut(s) 202
Esp3I CGTCTC 1 cut(s) 157
FaeI CATG 2 cut(s) 403, 488
FaiI YATR 4 cut(s) 141, 224, 401, 486
FatI CATG 2 cut(s) 399, 484
FblI GTMKAC 1 cut(s) 239
Fnu4HI GCNGC 5 cut(s) 76, 411, 495, 504, 507
FriOI GRGCYC 1 cut(s) 182
Fsp4HI GCNGC 5 cut(s) 76, 411, 495, 504, 507
FspBI CTAG 1 cut(s) 203
GlaI GCGC 2 cut(s) 310, 354
GluI GCNGC 5 cut(s) 76, 411, 495, 504, 507
GsuI CTGGAG 1 cut(s) 306
HaeIII GGCC 5 cut(s) 68, 201, 233, 296, 422
HapII CCGG 1 cut(s) 414
HgaI GACGC 3 cut(s) 107, 139, 416
HhaI GCGC 2 cut(s) 311, 355
Hin1I GRCGYC 1 cut(s) 99
Hin1II CATG 2 cut(s) 403, 488
Hin6I GCGC 2 cut(s) 309, 353
HinP1I GCGC 2 cut(s) 309, 353
HincII GTYRAC 1 cut(s) 240
HindII GTYRAC 1 cut(s) 240
HindIII AAGCTT 1 cut(s) 336
HpaII CCGG 1 cut(s) 414
HphI GGTGA 1 cut(s) 428
Hpy166II GTNNAC 1 cut(s) 240
Hpy188I TCNGA 4 cut(s) 169, 177, 252, 462
Hpy8I GTNNAC 1 cut(s) 240
Hpy99I CGWCG 1 cut(s) 410
HpyAV CCTTC 2 cut(s) 32, 223
HpyCH4III ACNGT 1 cut(s) 328
HpyCH4IV ACGT 2 cut(s) 192, 428
HpyCH4V TGCA 5 cut(s) 75, 123, 389, 484, 506
HpyF10VI GCNNNNNNNGC 5 cut(s) 352, 419, 500, 503, 512
HpyF3I CTNAG 1 cut(s) 370
HpySE526I ACGT 2 cut(s) 192, 428
Hsp92I GRCGYC 1 cut(s) 99
Hsp92II CATG 2 cut(s) 403, 488
HspAI GCGC 2 cut(s) 309, 353
LmnI GCTCC 2 cut(s) 177, 185
Lsp1109I GCAGC 4 cut(s) 87, 397, 490, 518
MaeI CTAG 1 cut(s) 203
MaeII ACGT 2 cut(s) 192, 428
MboII GAAGA 5 cut(s) 47, 50, 79, 147, 446
MfeI CAATTG 1 cut(s) 510
MhlI GDGCHC 1 cut(s) 182
MluCI AATT 1 cut(s) 510
MnlI CCTC 4 cut(s) 80, 183, 412, 468
MseI TTAA 2 cut(s) 162, 273
MspCI CTTAAG 1 cut(s) 272
MspI CCGG 1 cut(s) 414
MspR9I CCNGG 3 cut(s) 285, 293, 517
MunI CAATTG 1 cut(s) 510
MvaI CCWGG 3 cut(s) 285, 293, 517
MwoI GCNNNNNNNGC 5 cut(s) 352, 419, 500, 503, 512
NlaIII CATG 2 cut(s) 403, 488
NlaIV GGNNCC 2 cut(s) 171, 345
PceI AGGCCT 1 cut(s) 296
PkrI GCNGC 5 cut(s) 77, 412, 496, 505, 508
Psp124BI GAGCTC 1 cut(s) 182
Psp6I CCWGG 3 cut(s) 283, 291, 515
PspGI CCWGG 3 cut(s) 283, 291, 515
PspN4I GGNNCC 2 cut(s) 171, 345
PspPI GGNCC 3 cut(s) 67, 169, 199
PstI CTGCAG 2 cut(s) 77, 508
RsaI GTAC 1 cut(s) 40
RsaNI GTAC 1 cut(s) 39
SacI GAGCTC 1 cut(s) 182
SalI GTCGAC 1 cut(s) 238
SaqAI TTAA 2 cut(s) 162, 273
SatI GCNGC 5 cut(s) 76, 411, 495, 504, 507
Sau96I GGNCC 3 cut(s) 67, 169, 199
ScrFI CCNGG 3 cut(s) 285, 293, 517
SduI GDGCHC 1 cut(s) 182
SetI ASST 7 cut(s) 122, 182, 195, 286, 340, 371, 431
SfcI CTRYAG 2 cut(s) 73, 504
SinI GGWCC 1 cut(s) 169
SmlI CTYRAG 1 cut(s) 272
SmoI CTYRAG 1 cut(s) 272
Sse9I AATT 1 cut(s) 510
SseBI AGGCCT 1 cut(s) 296
SsiI CCGC 2 cut(s) 186, 494
SspMI CTAG 1 cut(s) 203
SstI GAGCTC 1 cut(s) 182
StuI AGGCCT 1 cut(s) 296
StyD4I CCNGG 3 cut(s) 283, 291, 515
StyI CCWWGG 1 cut(s) 202
TaaI ACNGT 1 cut(s) 328
TaiI ACGT 2 cut(s) 195, 431
TaqI TCGA 2 cut(s) 111, 239
TaqII GACCGA 1 cut(s) 257
TasI AATT 1 cut(s) 510
TauI GCSGC 1 cut(s) 497
Tru1I TTAA 2 cut(s) 162, 273
Tru9I TTAA 2 cut(s) 162, 273
TscAI CASTG 1 cut(s) 69
TseI GCWGC 4 cut(s) 75, 410, 503, 506
TspDTI ATGAA 2 cut(s) 17, 416
TspGWI ACGGA 1 cut(s) 479
TspRI CASTG 1 cut(s) 69
Vha464I CTTAAG 1 cut(s) 272
VpaK11BI GGWCC 1 cut(s) 169
XmaJI CCTAGG 1 cut(s) 202
XmiI GTMKAC 1 cut(s) 239
XspI CTAG 1 cut(s) 203
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.