Rorug04G0212000

Cell wall vacuolar inhibitor of fructosidase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0000921
Physical Location & Seq
Reverse (-)
36841257 .. 36842282
1026 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0212000.1

Sequence Viewer

Length: 1026 bp
ATGGGGATCAGCCTTGAAGATTTGAAAAGGAGAGAGTATAGCGAGTCTTTTGATGAGCTTTTAGCAAAAGCTAAAGAATTTGCAACAGACCGAGAGGAAGCACTTGAAATTTTGAGAAATTGGATTCTGTATGATTTGAGTTGTAGAAAATCCACAAGAGAAGAAAAAAAATCGACTAATATTGATCCGAAGGAGAAGGCTGAAAATCGACGTGACAGATGCAAAATAGTGATCAAGAAGATGAAAGCTAGCTCTACGAAGCCATGCAATGATATAAGAAAAGATCTGGTCCCTTGCAATGACGAAGATGAAGAAGCTTCTCATCCTTCTTCTGCAAGGAATAATGATCAGCAGATGAAAACTTATGCATGCTGCTCCAAAATTAGTGAAGATCAAGATAAATGTTTCAATAAACTCAAAGATGCTTCTTCTCCTCATTCTTCTACAAGGAACAAAAGGAAGAGCCAAAGTATTGAAGATCAACATGCATATTACTATACGGTCTATAACTATGCAGGTAAAGCTGGCAATTTGATGAACTTGATGGAGACGAAGAAGAAGGTGAAGAAGCCACGAGCTTTACCATTTGATGGTTCACCACCAGAGTTGCCTATGGATTTAAGAAACAAGATCGAATCCCTGCATGGCTCCGAGGCCAAACTAGTAATACAGAAGGAGATTACAAATACTGATGTGAACCGCCATCATGATCGTCTGAATATGCCGAGGAACCAAGTACTAACCATGGACGTTCTCAATGAACAGGAGCGAAAAAAGGTTTTTGAACATGGTTTGGAAGTGAGAGTGATACAACCATGTCTAGAAGAGAGAAACTTACATTTAAGGAAGTGGAATTCGCACAACGGCGGCTATTCCTATGTCTTAAACAGTGGTTGGAAAGATGTTGTTAGTAAGTGTGAGGATCATATTAGGCCTGGTAATACAATTCAGGTTTGGTCATTTCGAGTTGATGATCGCGGAGAGGTCTGGTTTGCCCTTGTGTGCCATGGGCCAAAAAAACTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

341

Amino Acids

39.67

Weight (kDa)

8.83

Isoelectric Point (pI)

51.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
At2g31720-like PF03754 207 - 305 5e-19 B3 domain-containing protein At2g31720-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 506
AccB7I CCANNNNNTGG 1 cut(s) 590
AccII CGCG 1 cut(s) 978
AciI CCGC 3 cut(s) 700, 867, 978
AclWI GGATC 3 cut(s) 14, 179, 930
AcsI RAATTY 3 cut(s) 77, 108, 853
AfaI GTAC 1 cut(s) 738
AfiI CCNNNNNNNGG 1 cut(s) 590
AgsI TTSAA 6 cut(s) 17, 25, 107, 409, 476, 785
AhlI ACTAGT 1 cut(s) 661
AjiI CACGTC 1 cut(s) 212
AjnI CCWGG 1 cut(s) 934
AluBI AGCT 7 cut(s) 58, 71, 248, 252, 317, 524, 578
AluI AGCT 7 cut(s) 58, 71, 248, 252, 317, 524, 578
Alw26I GTCTC 1 cut(s) 542
AlwI GGATC 3 cut(s) 14, 179, 930
AoxI GGCC 3 cut(s) 654, 932, 1010
ApeKI GCWGC 1 cut(s) 372
ApoI RAATTY 3 cut(s) 77, 108, 853
AspS9I GGNCC 2 cut(s) 289, 1010
AsuHPI GGTGA 2 cut(s) 574, 588
AsuNHI GCTAGC 1 cut(s) 248
AvaII GGWCC 1 cut(s) 289
BauI CACGAG 1 cut(s) 573
BbvI GCAGC 1 cut(s) 359
BccI CCATC 3 cut(s) 538, 584, 711
BceAI ACGGC 1 cut(s) 880
BciT130I CCWGG 1 cut(s) 936
BclI TGATCA 2 cut(s) 231, 346
BcoDI GTCTC 1 cut(s) 542
BcuI ACTAGT 1 cut(s) 661
BfaI CTAG 3 cut(s) 249, 662, 821
BfuAI ACCTGC 1 cut(s) 506
BglII AGATCT 1 cut(s) 283
BisI GCNGC 2 cut(s) 373, 868
BlsI GCNGC 2 cut(s) 374, 869
BmcAI AGTACT 1 cut(s) 738
Bme1390I CCNGG 1 cut(s) 936
Bme18I GGWCC 1 cut(s) 289
BmgBI CACGTC 1 cut(s) 212
BmgT120I GGNCC 2 cut(s) 289, 1010
BmiI GGNNCC 3 cut(s) 291, 649, 731
BmrFI CCNGG 1 cut(s) 936
BmsI GCATC 2 cut(s) 209, 412
BmtI GCTAGC 1 cut(s) 252
BsaJI CCNNGG 4 cut(s) 651, 725, 744, 1006
Bsc4I CCNNNNNNNGG 1 cut(s) 590
Bse3DI GCAATG 2 cut(s) 274, 304
BseBI CCWGG 1 cut(s) 936
BseDI CCNNGG 4 cut(s) 651, 725, 744, 1006
BseGI GGATG 1 cut(s) 322
BseLI CCNNNNNNNGG 1 cut(s) 590
BseMI GCAATG 2 cut(s) 274, 304
BseRI GAGGAG 1 cut(s) 423
BseXI GCAGC 1 cut(s) 359
Bsh1236I CGCG 1 cut(s) 978
BshFI GGCC 3 cut(s) 656, 934, 1012
BslFI GGGAC 1 cut(s) 275
BslI CCNNNNNNNGG 1 cut(s) 590
BsmAI GTCTC 1 cut(s) 542
BsmBI CGTCTC 1 cut(s) 542
BsmFI GGGAC 1 cut(s) 275
BsnI GGCC 3 cut(s) 656, 934, 1012
Bsp19I CCATGG 2 cut(s) 744, 1006
BspACI CCGC 3 cut(s) 700, 867, 978
BspANI GGCC 3 cut(s) 656, 934, 1012
BspFNI CGCG 1 cut(s) 978
BspHI TCATGA 1 cut(s) 706
BspLI GGNNCC 3 cut(s) 291, 649, 731
BspMI ACCTGC 1 cut(s) 506
BspOI GCTAGC 1 cut(s) 252
BspPI GGATC 3 cut(s) 14, 179, 930
BspQI GCTCTTC 1 cut(s) 455
BsrDI GCAATG 2 cut(s) 274, 304
BssECI CCNNGG 4 cut(s) 651, 725, 744, 1006
BssSI CACGAG 1 cut(s) 573
BssT1I CCWWGG 2 cut(s) 744, 1006
Bst2BI CACGAG 1 cut(s) 573
Bst2UI CCWGG 1 cut(s) 936
Bst4CI ACNGT 3 cut(s) 502, 890, 1023
Bst6I CTCTTC 2 cut(s) 455, 819
BstC8I GCNNGC 3 cut(s) 250, 370, 526
BstDSI CCRYGG 2 cut(s) 744, 1006
BstF5I GGATG 1 cut(s) 322
BstFNI CGCG 1 cut(s) 978
BstMAI GTCTC 1 cut(s) 542
BstMWI GCNNNNNNNGC 1 cut(s) 521
BstNI CCWGG 1 cut(s) 936
BstNSI RCATGY 2 cut(s) 372, 488
BstSCI CCNGG 1 cut(s) 934
BstUI CGCG 1 cut(s) 978
BstV1I GCAGC 1 cut(s) 359
BstX2I RGATCY 1 cut(s) 283
BstYI RGATCY 1 cut(s) 283
BsuRI GGCC 3 cut(s) 656, 934, 1012
BtgI CCRYGG 2 cut(s) 744, 1006
BtrI CACGTC 1 cut(s) 212
BtsCI GGATG 1 cut(s) 322
BtsIMutI CAGTG 1 cut(s) 895
BveI ACCTGC 1 cut(s) 506
Cac8I GCNNGC 3 cut(s) 250, 370, 526
CciI TCATGA 1 cut(s) 706
Cfr13I GGNCC 2 cut(s) 289, 1010
Csp6I GTAC 1 cut(s) 737
CspCI CAANNNNNGTGG 2 cut(s) 588, 623
CviAII CATG 9 cut(s) 264, 369, 485, 644, 707, 745, 788, 816, 1007
CviQI GTAC 1 cut(s) 737
Eam1104I CTCTTC 2 cut(s) 455, 819
EarI CTCTTC 2 cut(s) 455, 819
Eco130I CCWWGG 2 cut(s) 744, 1006
Eco147I AGGCCT 1 cut(s) 934
Eco47I GGWCC 1 cut(s) 289
EcoRI GAATTC 1 cut(s) 853
EcoRII CCWGG 1 cut(s) 934
EcoT14I CCWWGG 2 cut(s) 744, 1006
EcoT22I ATGCAT 2 cut(s) 370, 490
ErhI CCWWGG 2 cut(s) 744, 1006
Esp3I CGTCTC 1 cut(s) 542
FaeI CATG 9 cut(s) 267, 372, 488, 647, 710, 748, 791, 819, 1010
FaqI GGGAC 1 cut(s) 275
FatI CATG 9 cut(s) 263, 368, 484, 643, 706, 744, 787, 815, 1006
FbaI TGATCA 2 cut(s) 231, 346
Fnu4HI GCNGC 2 cut(s) 373, 868
FokI GGATG 1 cut(s) 309
Fsp4HI GCNGC 2 cut(s) 373, 868
FspBI CTAG 3 cut(s) 249, 662, 821
GluI GCNGC 2 cut(s) 373, 868
HaeIII GGCC 3 cut(s) 656, 934, 1012
Hin1II CATG 9 cut(s) 267, 372, 488, 647, 710, 748, 791, 819, 1010
HindIII AAGCTT 1 cut(s) 315
HinfI GANTC 3 cut(s) 44, 124, 635
HphI GGTGA 2 cut(s) 574, 588
Hpy166II GTNNAC 2 cut(s) 596, 697
Hpy188I TCNGA 3 cut(s) 189, 652, 717
Hpy188III TCNNGA 4 cut(s) 235, 395, 707, 821
Hpy8I GTNNAC 2 cut(s) 596, 697
Hpy99I CGWCG 1 cut(s) 213
HpyAV CCTTC 5 cut(s) 184, 190, 336, 553, 667
HpyCH4III ACNGT 3 cut(s) 502, 890, 1023
HpyCH4IV ACGT 2 cut(s) 211, 750
HpyCH4V TGCA 9 cut(s) 83, 222, 267, 297, 335, 368, 488, 515, 643
HpyF10VI GCNNNNNNNGC 1 cut(s) 521
HpySE526I ACGT 2 cut(s) 211, 750
Hsp92II CATG 9 cut(s) 267, 372, 488, 647, 710, 748, 791, 819, 1010
Ksp22I TGATCA 2 cut(s) 231, 346
LguI GCTCTTC 1 cut(s) 455
LmnI GCTCC 3 cut(s) 380, 653, 766
Lsp1109I GCAGC 1 cut(s) 359
LweI GCATC 2 cut(s) 209, 412
MaeI CTAG 3 cut(s) 249, 662, 821
MaeII ACGT 2 cut(s) 211, 750
MaeIII GTNAC 1 cut(s) 212
MflI RGATCY 1 cut(s) 283
MluCI AATT 7 cut(s) 77, 108, 118, 381, 529, 853, 945
MlyI GAGTC 1 cut(s) 53
MmeI TCCRAC 1 cut(s) 875
MnlI CCTC 6 cut(s) 88, 444, 646, 720, 913, 976
Mph1103I ATGCAT 2 cut(s) 370, 490
MseI TTAA 3 cut(s) 620, 842, 884
MspR9I CCNGG 1 cut(s) 936
MvaI CCWGG 1 cut(s) 936
MvnI CGCG 1 cut(s) 978
MwoI GCNNNNNNNGC 1 cut(s) 521
NcoI CCATGG 2 cut(s) 744, 1006
NheI GCTAGC 1 cut(s) 248
NlaIII CATG 9 cut(s) 267, 372, 488, 647, 710, 748, 791, 819, 1010
NlaIV GGNNCC 3 cut(s) 291, 649, 731
NmeAIII GCCGAG 1 cut(s) 750
NmuCI GTSAC 1 cut(s) 212
NsiI ATGCAT 2 cut(s) 370, 490
NspI RCATGY 2 cut(s) 372, 488
PaeI GCATGC 1 cut(s) 372
PagI TCATGA 1 cut(s) 706
PceI AGGCCT 1 cut(s) 934
PciSI GCTCTTC 1 cut(s) 455
PfeI GAWTC 2 cut(s) 124, 635
PflMI CCANNNNNTGG 1 cut(s) 590
PkrI GCNGC 2 cut(s) 374, 869
PleI GAGTC 1 cut(s) 52
PpsI GAGTC 1 cut(s) 52
Psp6I CCWGG 1 cut(s) 934
PspGI CCWGG 1 cut(s) 934
PspN4I GGNNCC 3 cut(s) 291, 649, 731
PspPI GGNCC 2 cut(s) 289, 1010
PsuI RGATCY 1 cut(s) 283
RsaI GTAC 1 cut(s) 738
RsaNI GTAC 1 cut(s) 737
SapI GCTCTTC 1 cut(s) 455
SaqAI TTAA 3 cut(s) 620, 842, 884
SatI GCNGC 2 cut(s) 373, 868
Sau96I GGNCC 2 cut(s) 289, 1010
ScaI AGTACT 1 cut(s) 738
SchI GAGTC 1 cut(s) 53
ScrFI CCNGG 1 cut(s) 936
SfaNI GCATC 2 cut(s) 209, 412
SinI GGWCC 1 cut(s) 289
SpeI ACTAGT 1 cut(s) 661
SphI GCATGC 1 cut(s) 372
Sse9I AATT 7 cut(s) 77, 108, 118, 381, 529, 853, 945
SseBI AGGCCT 1 cut(s) 934
SsiI CCGC 3 cut(s) 700, 867, 978
SspI AATATT 1 cut(s) 181
SspMI CTAG 3 cut(s) 249, 662, 821
StuI AGGCCT 1 cut(s) 934
StyD4I CCNGG 1 cut(s) 934
StyI CCWWGG 2 cut(s) 744, 1006
TaaI ACNGT 3 cut(s) 502, 890, 1023
TaiI ACGT 2 cut(s) 214, 753
TaqI TCGA 4 cut(s) 173, 208, 633, 964
TaqII GACCGA 1 cut(s) 105
TasI AATT 7 cut(s) 77, 108, 118, 381, 529, 853, 945
TatI WGTACW 1 cut(s) 736
TauI GCSGC 1 cut(s) 870
TfiI GAWTC 2 cut(s) 124, 635
Tru1I TTAA 3 cut(s) 620, 842, 884
Tru9I TTAA 3 cut(s) 620, 842, 884
TscAI CASTG 1 cut(s) 895
TseFI GTSAC 1 cut(s) 212
TseI GCWGC 1 cut(s) 372
Tsp45I GTSAC 1 cut(s) 212
TspDTI ATGAA 5 cut(s) 257, 324, 371, 551, 774
TspRI CASTG 1 cut(s) 895
Van91I CCANNNNNTGG 1 cut(s) 590
VpaK11BI GGWCC 1 cut(s) 289
XapI RAATTY 3 cut(s) 77, 108, 853
XbaI TCTAGA 1 cut(s) 820
XceI RCATGY 2 cut(s) 372, 488
XspI CTAG 3 cut(s) 249, 662, 821
ZrmI AGTACT 1 cut(s) 738
Zsp2I ATGCAT 2 cut(s) 370, 490
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.