Rh3DG280000

Cell wall vacuolar inhibitor of fructosidase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3D
OG0000921
Physical Location & Seq
Reverse (-)
28451208 .. 28466960
15753 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3DG280000.1

Sequence Viewer

Length: 462 bp
ATGGGCCAATGCAGCAGTGTTTTCTTTCCGATGGATGCGAAACTCATTGACCAAACGTGTAGAAAAACACCGGATTACAACCTTTGTACCTCAATTCTCAAATCTAGCCCCGATAAAGATCTTAGAGGTTTAGCTATCGTAGCTATTGATGCAGTTGGGTCTAAAGCAACTACTACAATGAACAGAATTAATGAGTTGCTTAAACAAAGCCCAAAAGATACTGCCTTAAATCATTGTCATGATATGTACAATGTAATTTTAAATTCATATGTTCAAGGAGCCAGGGATACGCTTAAGCGGGGTTTCTCTTCTCATATTGCAACCTCAAGCATGAGTCTCACTCCCGATACTGCAAGGTCATGCGAGGAAGCTTTTAAAGGCAGGTCTCCTCTTACTAATTTGAACAAAGATGTCGAGAATATTGCGGCAGTGGCTGGAGCAGTGGCGGGGCAATTGCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

153

Amino Acids

16.51

Weight (kDa)

8.29

Isoelectric Point (pI)

48.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 14 - 143 1.1e-18 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 372
AciI CCGC 3 cut(s) 298, 425, 446
AcsI RAATTY 1 cut(s) 262
AfaI GTAC 2 cut(s) 88, 248
AflII CTTAAG 1 cut(s) 293
AflIII ACRYGT 1 cut(s) 56
AgsI TTSAA 2 cut(s) 275, 403
AjnI CCWGG 1 cut(s) 281
AluBI AGCT 3 cut(s) 134, 143, 371
AluI AGCT 3 cut(s) 134, 143, 371
Alw26I GTCTC 2 cut(s) 341, 390
AlwNI CAGNNNCTG 1 cut(s) 434
AoxI GGCC 1 cut(s) 4
ApeKI GCWGC 1 cut(s) 12
ApoI RAATTY 1 cut(s) 262
AseI ATTAAT 1 cut(s) 189
AspS9I GGNCC 1 cut(s) 4
BaeI ACNNNNGTAYC 2 cut(s) 70, 103
BbvI GCAGC 1 cut(s) 24
BccI CCATC 1 cut(s) 25
BcgI CGANNNNNNTGC 2 cut(s) 404, 438
BciT130I CCWGG 1 cut(s) 283
BciVI GTATCC 1 cut(s) 280
BcoDI GTCTC 2 cut(s) 341, 390
BfaI CTAG 1 cut(s) 105
BfrI CTTAAG 1 cut(s) 293
BfuAI ACCTGC 1 cut(s) 372
BfuI GTATCC 1 cut(s) 280
BglII AGATCT 1 cut(s) 118
BisI GCNGC 2 cut(s) 13, 426
BlsI GCNGC 2 cut(s) 14, 427
Bme1390I CCNGG 1 cut(s) 283
BmgT120I GGNCC 1 cut(s) 4
BmiI GGNNCC 1 cut(s) 280
BmrFI CCNGG 1 cut(s) 283
BmsI GCATC 2 cut(s) 25, 139
BplI GAGNNNNNCTC 2 cut(s) 325, 357
BpmI CTGGAG 1 cut(s) 456
BpuEI CTTGAG 1 cut(s) 310
BsaBI GATNNNNATC 1 cut(s) 117
BsaI GGTCTC 1 cut(s) 390
BsaJI CCNNGG 1 cut(s) 282
BsaWI WCCGGW 1 cut(s) 70
Bse8I GATNNNNATC 1 cut(s) 117
BseBI CCWGG 1 cut(s) 283
BseDI CCNNGG 1 cut(s) 282
BseGI GGATG 1 cut(s) 40
BseJI GATNNNNATC 1 cut(s) 117
BseRI GAGGAG 1 cut(s) 378
BseXI GCAGC 1 cut(s) 24
BshFI GGCC 1 cut(s) 6
BsiSI CCGG 1 cut(s) 71
BsmAI GTCTC 2 cut(s) 341, 390
BsnI GGCC 1 cut(s) 6
Bso31I GGTCTC 1 cut(s) 390
Bsp1407I TGTACA 1 cut(s) 246
Bsp143I GATC 1 cut(s) 118
BspACI CCGC 3 cut(s) 298, 425, 446
BspANI GGCC 1 cut(s) 6
BspHI TCATGA 1 cut(s) 238
BspLI GGNNCC 1 cut(s) 280
BspMI ACCTGC 1 cut(s) 372
BspTI CTTAAG 1 cut(s) 293
BspTNI GGTCTC 1 cut(s) 390
BsrGI TGTACA 1 cut(s) 246
BssECI CCNNGG 1 cut(s) 282
BssMI GATC 1 cut(s) 118
Bst2UI CCWGG 1 cut(s) 283
Bst6I CTCTTC 1 cut(s) 313
BstAFI CTTAAG 1 cut(s) 293
BstAUI TGTACA 1 cut(s) 246
BstDEI CTNAG 1 cut(s) 122
BstF5I GGATG 1 cut(s) 40
BstKTI GATC 1 cut(s) 121
BstMAI GTCTC 2 cut(s) 341, 390
BstMBI GATC 1 cut(s) 118
BstMWI GCNNNNNNNGC 4 cut(s) 12, 140, 149, 431
BstNI CCWGG 1 cut(s) 283
BstSCI CCNGG 1 cut(s) 281
BstV1I GCAGC 1 cut(s) 24
BstX2I RGATCY 1 cut(s) 118
BstYI RGATCY 1 cut(s) 118
BsuI GTATCC 1 cut(s) 280
BsuRI GGCC 1 cut(s) 6
BtsCI GGATG 1 cut(s) 40
BtsI GCAGTG 3 cut(s) 22, 435, 447
BtsIMutI CAGTG 3 cut(s) 22, 435, 447
BveI ACCTGC 1 cut(s) 372
CaiI CAGNNNCTG 1 cut(s) 434
CciI TCATGA 1 cut(s) 238
Cfr13I GGNCC 1 cut(s) 4
Csp6I GTAC 2 cut(s) 87, 247
CviAII CATG 3 cut(s) 239, 331, 360
CviJI RGCY 8 cut(s) 6, 108, 134, 143, 210, 281, 371, 434
CviKI_1 RGCY 8 cut(s) 6, 108, 134, 143, 210, 281, 371, 434
CviQI GTAC 2 cut(s) 87, 247
DdeI CTNAG 1 cut(s) 122
DpnI GATC 1 cut(s) 120
DpnII GATC 1 cut(s) 118
DraI TTTAAA 2 cut(s) 261, 376
Eam1104I CTCTTC 1 cut(s) 313
EarI CTCTTC 1 cut(s) 313
Eco31I GGTCTC 1 cut(s) 390
EcoRII CCWGG 1 cut(s) 281
FaeI CATG 3 cut(s) 242, 334, 363
FaiI YATR 7 cut(s) 240, 245, 268, 270, 315, 332, 361
FatI CATG 3 cut(s) 238, 330, 359
FauI CCCGC 2 cut(s) 291, 439
FauNDI CATATG 1 cut(s) 268
Fnu4HI GCNGC 2 cut(s) 13, 426
FokI GGATG 1 cut(s) 47
Fsp4HI GCNGC 2 cut(s) 13, 426
FspBI CTAG 1 cut(s) 105
GluI GCNGC 2 cut(s) 13, 426
GsuI CTGGAG 1 cut(s) 456
HaeIII GGCC 1 cut(s) 6
HapII CCGG 1 cut(s) 71
Hin1II CATG 3 cut(s) 242, 334, 363
HindIII AAGCTT 1 cut(s) 369
HinfI GANTC 1 cut(s) 334
HpaII CCGG 1 cut(s) 71
Hpy188I TCNGA 1 cut(s) 30
Hpy188III TCNNGA 3 cut(s) 239, 344, 415
HpyCH4IV ACGT 1 cut(s) 56
HpyCH4V TGCA 4 cut(s) 12, 152, 320, 353
HpyF10VI GCNNNNNNNGC 4 cut(s) 12, 140, 149, 431
HpyF3I CTNAG 1 cut(s) 122
HpySE526I ACGT 1 cut(s) 56
Hsp92II CATG 3 cut(s) 242, 334, 363
Kzo9I GATC 1 cut(s) 118
LmnI GCTCC 2 cut(s) 278, 437
LpnPI CCDG 5 cut(s) 84, 268, 295, 367, 420
Lsp1109I GCAGC 1 cut(s) 24
LweI GCATC 2 cut(s) 25, 139
MaeI CTAG 1 cut(s) 105
MaeII ACGT 1 cut(s) 56
MalI GATC 1 cut(s) 120
MboI GATC 1 cut(s) 118
MboII GAAGA 1 cut(s) 300
MfeI CAATTG 1 cut(s) 452
MflI RGATCY 1 cut(s) 118
MluCI AATT 6 cut(s) 93, 186, 255, 262, 397, 452
MlyI GAGTC 1 cut(s) 343
MnlI CCTC 5 cut(s) 100, 119, 334, 358, 399
MseI TTAA 6 cut(s) 189, 201, 227, 260, 294, 375
MslI CAYNNNNRTG 1 cut(s) 237
MspCI CTTAAG 1 cut(s) 293
MspI CCGG 1 cut(s) 71
MspR9I CCNGG 1 cut(s) 283
MunI CAATTG 1 cut(s) 452
MvaI CCWGG 1 cut(s) 283
MwoI GCNNNNNNNGC 4 cut(s) 12, 140, 149, 431
NdeI CATATG 1 cut(s) 268
NdeII GATC 1 cut(s) 118
NlaIII CATG 3 cut(s) 242, 334, 363
NlaIV GGNNCC 1 cut(s) 280
PagI TCATGA 1 cut(s) 238
PkrI GCNGC 2 cut(s) 14, 427
PleI GAGTC 1 cut(s) 342
PpsI GAGTC 1 cut(s) 342
PshBI ATTAAT 1 cut(s) 189
Psp6I CCWGG 1 cut(s) 281
PspGI CCWGG 1 cut(s) 281
PspN4I GGNNCC 1 cut(s) 280
PspPI GGNCC 1 cut(s) 4
PstNI CAGNNNCTG 1 cut(s) 434
PsuI RGATCY 1 cut(s) 118
RsaI GTAC 2 cut(s) 88, 248
RsaNI GTAC 2 cut(s) 87, 247
RseI CAYNNNNRTG 1 cut(s) 237
SaqAI TTAA 6 cut(s) 189, 201, 227, 260, 294, 375
SatI GCNGC 2 cut(s) 13, 426
Sau3AI GATC 1 cut(s) 118
Sau96I GGNCC 1 cut(s) 4
SchI GAGTC 1 cut(s) 343
ScrFI CCNGG 1 cut(s) 283
SfaNI GCATC 2 cut(s) 25, 139
SmiMI CAYNNNNRTG 1 cut(s) 237
SmlI CTYRAG 2 cut(s) 293, 325
SmoI CTYRAG 2 cut(s) 293, 325
Sse9I AATT 6 cut(s) 93, 186, 255, 262, 397, 452
SsiI CCGC 3 cut(s) 298, 425, 446
SspI AATATT 1 cut(s) 421
SspMI CTAG 1 cut(s) 105
StyD4I CCNGG 1 cut(s) 281
TaiI ACGT 1 cut(s) 59
TaqI TCGA 1 cut(s) 414
TasI AATT 6 cut(s) 93, 186, 255, 262, 397, 452
TatI WGTACW 1 cut(s) 246
TauI GCSGC 1 cut(s) 428
Tru1I TTAA 6 cut(s) 189, 201, 227, 260, 294, 375
Tru9I TTAA 6 cut(s) 189, 201, 227, 260, 294, 375
TscAI CASTG 3 cut(s) 22, 435, 447
TseI GCWGC 1 cut(s) 12
TspDTI ATGAA 2 cut(s) 194, 255
TspRI CASTG 3 cut(s) 22, 435, 447
Vha464I CTTAAG 1 cut(s) 293
VspI ATTAAT 1 cut(s) 189
XapI RAATTY 1 cut(s) 262
XspI CTAG 1 cut(s) 105
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.