Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0000921

Physical Location & Seq

Genomic Coordinates Reverse (-)

33768499 .. 33768939

Gene Structure 441 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1249700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 441 bp

ATGGATGCCAATCTGATCGCACAAACATGCAACCAAACACCATATCCCGATCTTTGCGTCTCTACACTTCAATCAGACCCTCGAAGCGCCAAAGCAGATGTCAAAGGCTTAGGCATCATAATGGTTGATGCAGTTAAGGCTAAGGCACACGAAGCTAGTCTCCAAGTCGAGGAGCTGGTCGATAAAGATCCGGGAAATGTAGCCTTACAAGACTGCAGATTTAACTACGACGATATTTTCCAACTTGATATTCCCCAAGCTGTTGAAGCTTTCGATAAAGATGATTATAAGTCAGCAGAGCTAGGCATGACCAATGTCGTCGCAAACGAAGATTCATGCGAAAGACGTTTTTCCGGCGGAAGTCCCTTCAAAGATGAAAGCCAAGCTCTCCATGATGTTGCTTATGTGGCTGCAGCAATTGCCAAAGTATTGCGCTCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PMEI

Protein Analysis

147

Amino Acids

15.88

Weight (kDa)

4.44

Isoelectric Point (pI)

39.63

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PMEI	PF04043	4 - 139	7.3e-23	Plant invertase/pectin methylesterase inhibitor

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0000921)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G47960 AT1G47960 AT3G17140
fragaria_vesca	FvH4_1g05890 FvH4_4g14140 FvH4_4g20440 FvH4_5g04330 FvH4_6g33790
malus_domestica	MD03G1248700.v1.1 MD03G1249600.v1.1 MD03G1249700.v1.1 MD03G1250000.v1.1 MD13G1149700.v1.1 MD13G1150200.v1.1 MD13G1150300.v1.1 MD13G1150400.v1.1 MD16G1150000.v1.1 MD16G1150100.v1.1 MD16G1150600.v1.1
prunus_persica	Prupe.1G114500_v2.0.a1 Prupe.3G146800_v2.0.a1 Prupe.5G112600_v2.0.a1
pyrus_communis	pycom16g12860
rosa_chinensis	RchiOBHm_Chr2g0143761 RchiOBHm_Chr2g0144321 RchiOBHm_Chr3g0482891 RchiOBHm_Chr4g0399701 RchiOBHm_Chr4g0426481 RchiOBHm_Chr7g0200071
rosa_laevigata	RLG00000020073
rosa_multiflora	Rmu_sc0000590.1_g000011 Rmu_sc0003475.1_g000029 Rmu_ssc0000118.1_g000013 Rmu_ssc0000175.1_g000025
rosa_roxburghii	Rroxscaffold_2G00101760 Rroxscaffold_5G00344640 Rroxscaffold_5G00368490 Rroxscaffold_6G00398850 Rroxscaffold_6G00399210
rosa_rugosa	Rorug02G0382900 Rorug02G0386000 Rorug03G0196600 Rorug04G0018400 Rorug04G0211400 Rorug04G0212000
rosa_samantha	Rh2AG435200 Rh2AG438200 Rh2BG448600 Rh2CG425000 Rh2DG458200 Rh3BG287200 Rh3CG280900 Rh3CG284500 Rh3DG276900 Rh3DG277000 Rh3DG280000 Rh4BG274400 Rh4CG104700 Rh4CG288400 Rh4DG088700 Rh7DG186900
rosa_wichuraiana	Rw3G022400 Rw3G022410 Rw4G007920

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	288
AciI	CCGC	1 cut(s)	357
AclWI	GGATC	1 cut(s)	182
AfiI	CCNNNNNNNGG	1 cut(s)	169
AgsI	TTSAA	3 cut(s)	71, 266, 370
AjuI	GAANNNNNNNTTGG	2 cut(s)	234, 266
AluBI	AGCT	6 cut(s)	155, 175, 260, 269, 301, 386
AluI	AGCT	6 cut(s)	155, 175, 260, 269, 301, 386
Alw26I	GTCTC	2 cut(s)	64, 164
AlwI	GGATC	1 cut(s)	182
ApeKI	GCWGC	2 cut(s)	410, 413
ArsI	GACNNNNNNTTYG	2 cut(s)	84, 116
AspLEI	GCGC	2 cut(s)	89, 435
AsuC2I	CCSGG	1 cut(s)	192
BbvI	GCAGC	2 cut(s)	397, 425
BcnI	CCSGG	1 cut(s)	192
BcoDI	GTCTC	2 cut(s)	64, 164
BfaI	CTAG	2 cut(s)	156, 302
BfmI	CTRYAG	2 cut(s)	214, 411
BfoI	RGCGCY	1 cut(s)	90
BisI	GCNGC	2 cut(s)	411, 414
BlsI	GCNGC	2 cut(s)	412, 415
Bme1390I	CCNGG	1 cut(s)	192
BmrFI	CCNGG	1 cut(s)	192
BmsI	GCATC	2 cut(s)	118, 123
BoxI	GACNNNNGTC	1 cut(s)	314
Bpu10I	CCTNAGC	2 cut(s)	109, 141
BpuMI	CCSGG	1 cut(s)	192
BsaBI	GATNNNNATC	2 cut(s)	9, 186
Bsc4I	CCNNNNNNNGG	1 cut(s)	169
Bse8I	GATNNNNATC	2 cut(s)	9, 186
BseGI	GGATG	1 cut(s)	10
BseJI	GATNNNNATC	2 cut(s)	9, 186
BseLI	CCNNNNNNNGG	1 cut(s)	169
BseRI	GAGGAG	1 cut(s)	185
BseXI	GCAGC	2 cut(s)	397, 425
BsiSI	CCGG	2 cut(s)	191, 354
BslFI	GGGAC	1 cut(s)	348
BslI	CCNNNNNNNGG	1 cut(s)	169
BsmAI	GTCTC	2 cut(s)	64, 164
BsmBI	CGTCTC	1 cut(s)	64
BsmFI	GGGAC	1 cut(s)	348
Bsp143I	GATC	3 cut(s)	15, 49, 187
BspACI	CCGC	1 cut(s)	357
BspMAI	CTGCAG	2 cut(s)	218, 415
BspPI	GGATC	1 cut(s)	182
BssMI	GATC	3 cut(s)	15, 49, 187
BstAPI	GCANNNNNTGC	1 cut(s)	419
BstDEI	CTNAG	2 cut(s)	109, 141
BstF5I	GGATG	1 cut(s)	10
BstH2I	RGCGCY	1 cut(s)	90
BstHHI	GCGC	2 cut(s)	89, 435
BstKTI	GATC	3 cut(s)	18, 52, 190
BstMAI	GTCTC	2 cut(s)	64, 164
BstMBI	GATC	3 cut(s)	15, 49, 187
BstMWI	GCNNNNNNNGC	5 cut(s)	137, 152, 266, 407, 419
BstNSI	RCATGY	1 cut(s)	30
BstPAI	GACNNNNGTC	1 cut(s)	314
BstSCI	CCNGG	1 cut(s)	190
BstSFI	CTRYAG	2 cut(s)	214, 411
BstV1I	GCAGC	2 cut(s)	397, 425
BstX2I	RGATCY	1 cut(s)	187
BstYI	RGATCY	1 cut(s)	187
BtsCI	GGATG	1 cut(s)	10
CfoI	GCGC	2 cut(s)	89, 435
CseI	GACGC	1 cut(s)	46
CviAII	CATG	4 cut(s)	27, 307, 336, 392
DdeI	CTNAG	2 cut(s)	109, 141
DpnI	GATC	3 cut(s)	17, 51, 189
DpnII	GATC	3 cut(s)	15, 49, 187
EciI	GGCGGA	1 cut(s)	372
Esp3I	CGTCTC	1 cut(s)	64
FaeI	CATG	4 cut(s)	30, 310, 339, 395
FaiI	YATR	8 cut(s)	28, 43, 119, 288, 308, 337, 393, 405
FaqI	GGGAC	1 cut(s)	348
FatI	CATG	4 cut(s)	26, 306, 335, 391
Fnu4HI	GCNGC	2 cut(s)	411, 414
FokI	GGATG	1 cut(s)	17
Fsp4HI	GCNGC	2 cut(s)	411, 414
FspBI	CTAG	2 cut(s)	156, 302
GlaI	GCGC	2 cut(s)	88, 434
GluI	GCNGC	2 cut(s)	411, 414
HaeII	RGCGCY	1 cut(s)	90
HapII	CCGG	2 cut(s)	191, 354
HgaI	GACGC	1 cut(s)	46
HhaI	GCGC	2 cut(s)	89, 435
Hin1II	CATG	4 cut(s)	30, 310, 339, 395
Hin6I	GCGC	2 cut(s)	87, 433
HinP1I	GCGC	2 cut(s)	87, 433
HindIII	AAGCTT	1 cut(s)	267
HinfI	GANTC	1 cut(s)	332
HpaII	CCGG	2 cut(s)	191, 354
Hpy188I	TCNGA	2 cut(s)	15, 76
Hpy188III	TCNNGA	2 cut(s)	47, 438
Hpy99I	CGWCG	2 cut(s)	233, 323
HpyAV	CCTTC	1 cut(s)	376
HpyCH4IV	ACGT	1 cut(s)	346
HpyCH4V	TGCA	4 cut(s)	30, 131, 216, 413
HpyF10VI	GCNNNNNNNGC	5 cut(s)	137, 152, 266, 407, 419
HpyF3I	CTNAG	2 cut(s)	109, 141
HpySE526I	ACGT	1 cut(s)	346
Hsp92II	CATG	4 cut(s)	30, 310, 339, 395
HspAI	GCGC	2 cut(s)	87, 433
Kzo9I	GATC	3 cut(s)	15, 49, 187
LmnI	GCTCC	1 cut(s)	172
LpnPI	CCDG	3 cut(s)	161, 204, 367
Lsp1109I	GCAGC	2 cut(s)	397, 425
LweI	GCATC	2 cut(s)	118, 123
MaeI	CTAG	2 cut(s)	156, 302
MaeII	ACGT	1 cut(s)	346
MalI	GATC	3 cut(s)	17, 51, 189
MboI	GATC	3 cut(s)	15, 49, 187
MboII	GAAGA	1 cut(s)	341
MfeI	CAATTG	1 cut(s)	417
MflI	RGATCY	1 cut(s)	187
MluCI	AATT	1 cut(s)	417
MmeI	TCCRAC	1 cut(s)	265
MnlI	CCTC	2 cut(s)	90, 163
MseI	TTAA	2 cut(s)	135, 222
MslI	CAYNNNNRTG	2 cut(s)	25, 119
MspI	CCGG	2 cut(s)	191, 354
MspR9I	CCNGG	1 cut(s)	192
MunI	CAATTG	1 cut(s)	417
MwoI	GCNNNNNNNGC	5 cut(s)	137, 152, 266, 407, 419
NciI	CCSGG	1 cut(s)	192
NdeII	GATC	3 cut(s)	15, 49, 187
NlaIII	CATG	4 cut(s)	30, 310, 339, 395
NspI	RCATGY	1 cut(s)	30
PcsI	WCGNNNNNNNCGW	1 cut(s)	324
PfeI	GAWTC	1 cut(s)	332
PfoI	TCCNGGA	1 cut(s)	190
PkrI	GCNGC	2 cut(s)	412, 415
PshAI	GACNNNNGTC	1 cut(s)	314
PsiI	TTATAA	1 cut(s)	288
PstI	CTGCAG	2 cut(s)	218, 415
PsuI	RGATCY	1 cut(s)	187
RseI	CAYNNNNRTG	2 cut(s)	25, 119
SaqAI	TTAA	2 cut(s)	135, 222
SatI	GCNGC	2 cut(s)	411, 414
Sau3AI	GATC	3 cut(s)	15, 49, 187
ScrFI	CCNGG	1 cut(s)	192
SetI	ASST	7 cut(s)	157, 177, 262, 271, 303, 349, 388
SfaNI	GCATC	2 cut(s)	118, 123
SfcI	CTRYAG	2 cut(s)	214, 411
SmiMI	CAYNNNNRTG	2 cut(s)	25, 119
Sse9I	AATT	1 cut(s)	417
SsiI	CCGC	1 cut(s)	357
SspMI	CTAG	2 cut(s)	156, 302
StyD4I	CCNGG	1 cut(s)	190
TaiI	ACGT	1 cut(s)	349
TaqI	TCGA	4 cut(s)	82, 168, 180, 273
TasI	AATT	1 cut(s)	417
TfiI	GAWTC	1 cut(s)	332
Tru1I	TTAA	2 cut(s)	135, 222
Tru9I	TTAA	2 cut(s)	135, 222
TseI	GCWGC	2 cut(s)	410, 413
TspDTI	ATGAA	2 cut(s)	324, 390
XceI	RCATGY	1 cut(s)	30
XspI	CTAG	2 cut(s)	156, 302

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.