Rh4DG088700

Cell wall vacuolar inhibitor of fructosidase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0000921
Physical Location & Seq
Forward (+)
13496514 .. 13496963
450 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG088700.1

Sequence Viewer

Length: 450 bp
ATGGATGCCAATCTCATTACCAAAACATGTAAGGAAACGCCAAGTTACGATCTTTGTGATTCTTCTCCTCAATCAGACCCTTGGAGCTTCGATGCAGATGTCAAAGGGTTAGCCATATTAATGGTCGATGTTGTTAAGGCTAAGGCAACTGATACTGTAAACCATATCAATGATCTTCTTAAACAGACGTCGGGAGCTCAACCCTTAAGTTCTTGCCTCGATGTATATAATGTAGTTATACAGGCTAAAGTCCCAAAAGCTAGTGAAGCTCTGTCTAAAGGTGGCCCTAAGTTTGCAGAGCAAGGCATGAATGATGTTGGCCTGGGGGCAGATTTATGTGAAAATAGTTTTTCGGGCAGCTCATCTCCTCTCACATATTTGAACAAAACTGTTAGGGATGTTGCGGGTGTGGCTTCAGCAATTGTTCAGCATTTTGATATGCATATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

149

Amino Acids

15.84

Weight (kDa)

4.83

Isoelectric Point (pI)

43.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 4 - 140 1.7e-15 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 191
AciI CCGC 1 cut(s) 404
AcuI CTGAAG 1 cut(s) 399
AcyI GRCGYC 1 cut(s) 188
AflII CTTAAG 1 cut(s) 205
AflIII ACRYGT 1 cut(s) 26
AgsI TTSAA 1 cut(s) 382
AjnI CCWGG 1 cut(s) 321
AluBI AGCT 5 cut(s) 87, 197, 260, 269, 360
AluI AGCT 5 cut(s) 87, 197, 260, 269, 360
Alw21I GWGCWC 1 cut(s) 199
AoxI GGCC 2 cut(s) 283, 319
ApeKI GCWGC 1 cut(s) 357
AseI ATTAAT 1 cut(s) 119
AspS9I GGNCC 1 cut(s) 284
BanII GRGCYC 1 cut(s) 199
Bbv12I GWGCWC 1 cut(s) 199
BbvI GCAGC 1 cut(s) 369
BciT130I CCWGG 1 cut(s) 323
BfaI CTAG 1 cut(s) 261
BfrI CTTAAG 1 cut(s) 205
BisI GCNGC 1 cut(s) 358
BlsI GCNGC 1 cut(s) 359
Bme1390I CCNGG 1 cut(s) 323
BmgT120I GGNCC 1 cut(s) 284
BmrFI CCNGG 1 cut(s) 323
BmsI GCATC 1 cut(s) 82
Bpu10I CCTNAGC 1 cut(s) 141
BsaBI GATNNNNATC 1 cut(s) 9
BsaHI GRCGYC 1 cut(s) 188
BsaJI CCNNGG 2 cut(s) 80, 322
Bse8I GATNNNNATC 1 cut(s) 9
BseBI CCWGG 1 cut(s) 323
BseDI CCNNGG 2 cut(s) 80, 322
BseGI GGATG 2 cut(s) 10, 403
BseJI GATNNNNATC 1 cut(s) 9
BseRI GAGGAG 2 cut(s) 57, 357
BseXI GCAGC 1 cut(s) 369
BshFI GGCC 2 cut(s) 285, 321
BsiHKAI GWGCWC 1 cut(s) 199
BslFI GGGAC 1 cut(s) 236
BsmFI GGGAC 1 cut(s) 236
BsnI GGCC 2 cut(s) 285, 321
Bsp1286I GDGCHC 1 cut(s) 199
Bsp143I GATC 2 cut(s) 49, 172
BspACI CCGC 1 cut(s) 404
BspANI GGCC 2 cut(s) 285, 321
BspTI CTTAAG 1 cut(s) 205
BssECI CCNNGG 2 cut(s) 80, 322
BssMI GATC 2 cut(s) 49, 172
BssNI GRCGYC 1 cut(s) 188
BssT1I CCWWGG 1 cut(s) 80
Bst2UI CCWGG 1 cut(s) 323
Bst4CI ACNGT 2 cut(s) 157, 391
BstACI GRCGYC 1 cut(s) 188
BstAFI CTTAAG 1 cut(s) 205
BstDEI CTNAG 2 cut(s) 141, 288
BstF5I GGATG 2 cut(s) 10, 403
BstKTI GATC 2 cut(s) 52, 175
BstMBI GATC 2 cut(s) 49, 172
BstMWI GCNNNNNNNGC 2 cut(s) 266, 410
BstNI CCWGG 1 cut(s) 323
BstNSI RCATGY 1 cut(s) 30
BstSCI CCNGG 1 cut(s) 321
BstV1I GCAGC 1 cut(s) 369
BstXI CCANNNNNNTGG 1 cut(s) 121
BsuRI GGCC 2 cut(s) 285, 321
BtsCI GGATG 2 cut(s) 10, 403
Cfr13I GGNCC 1 cut(s) 284
CviAII CATG 2 cut(s) 27, 307
DdeI CTNAG 2 cut(s) 141, 288
DpnI GATC 2 cut(s) 51, 174
DpnII GATC 2 cut(s) 49, 172
Ecl136II GAGCTC 1 cut(s) 197
Eco130I CCWWGG 1 cut(s) 80
Eco24I GRGCYC 1 cut(s) 199
Eco53kI GAGCTC 1 cut(s) 197
Eco57I CTGAAG 1 cut(s) 399
EcoICRI GAGCTC 1 cut(s) 197
EcoRII CCWGG 1 cut(s) 321
EcoT14I CCWWGG 1 cut(s) 80
EcoT22I ATGCAT 1 cut(s) 444
EcoT38I GRGCYC 1 cut(s) 199
ErhI CCWWGG 1 cut(s) 80
FaeI CATG 2 cut(s) 30, 310
FaqI GGGAC 1 cut(s) 236
FatI CATG 2 cut(s) 26, 306
FauI CCCGC 1 cut(s) 397
FauNDI CATATG 1 cut(s) 444
Fnu4HI GCNGC 1 cut(s) 358
FokI GGATG 2 cut(s) 17, 410
FriOI GRGCYC 1 cut(s) 199
Fsp4HI GCNGC 1 cut(s) 358
FspBI CTAG 1 cut(s) 261
GluI GCNGC 1 cut(s) 358
HaeIII GGCC 2 cut(s) 285, 321
Hin1I GRCGYC 1 cut(s) 188
Hin1II CATG 2 cut(s) 30, 310
HinfI GANTC 1 cut(s) 59
Hpy166II GTNNAC 1 cut(s) 160
Hpy188I TCNGA 1 cut(s) 76
Hpy188III TCNNGA 1 cut(s) 192
Hpy8I GTNNAC 1 cut(s) 160
Hpy99I CGWCG 1 cut(s) 193
HpyCH4III ACNGT 2 cut(s) 157, 391
HpyCH4IV ACGT 1 cut(s) 188
HpyCH4V TGCA 3 cut(s) 95, 296, 442
HpyF10VI GCNNNNNNNGC 2 cut(s) 266, 410
HpyF3I CTNAG 2 cut(s) 141, 288
HpySE526I ACGT 1 cut(s) 188
Hsp92I GRCGYC 1 cut(s) 188
Hsp92II CATG 2 cut(s) 30, 310
Kzo9I GATC 2 cut(s) 49, 172
LmnI GCTCC 2 cut(s) 84, 194
LpnPI CCDG 3 cut(s) 227, 308, 335
Lsp1109I GCAGC 1 cut(s) 369
LweI GCATC 1 cut(s) 82
MaeI CTAG 1 cut(s) 261
MaeII ACGT 1 cut(s) 188
MaeIII GTNAC 1 cut(s) 44
MalI GATC 2 cut(s) 51, 174
MboI GATC 2 cut(s) 49, 172
MboII GAAGA 2 cut(s) 54, 167
MfeI CAATTG 1 cut(s) 420
MhlI GDGCHC 1 cut(s) 199
MluCI AATT 1 cut(s) 420
MnlI CCTC 3 cut(s) 78, 227, 378
Mph1103I ATGCAT 1 cut(s) 444
MseI TTAA 4 cut(s) 119, 135, 180, 206
MslI CAYNNNNRTG 2 cut(s) 119, 168
MspCI CTTAAG 1 cut(s) 205
MspR9I CCNGG 1 cut(s) 323
MunI CAATTG 1 cut(s) 420
MvaI CCWGG 1 cut(s) 323
MwoI GCNNNNNNNGC 2 cut(s) 266, 410
NdeI CATATG 1 cut(s) 444
NdeII GATC 2 cut(s) 49, 172
NlaIII CATG 2 cut(s) 30, 310
NsiI ATGCAT 1 cut(s) 444
NspI RCATGY 1 cut(s) 30
PciI ACATGT 1 cut(s) 26
PfeI GAWTC 1 cut(s) 59
PkrI GCNGC 1 cut(s) 359
PscI ACATGT 1 cut(s) 26
PshBI ATTAAT 1 cut(s) 119
Psp124BI GAGCTC 1 cut(s) 199
Psp6I CCWGG 1 cut(s) 321
PspGI CCWGG 1 cut(s) 321
PspPI GGNCC 1 cut(s) 284
RseI CAYNNNNRTG 2 cut(s) 119, 168
SacI GAGCTC 1 cut(s) 199
SaqAI TTAA 4 cut(s) 119, 135, 180, 206
SatI GCNGC 1 cut(s) 358
Sau3AI GATC 2 cut(s) 49, 172
Sau96I GGNCC 1 cut(s) 284
ScrFI CCNGG 1 cut(s) 323
SduI GDGCHC 1 cut(s) 199
SetI ASST 7 cut(s) 89, 191, 199, 262, 271, 283, 362
SfaNI GCATC 1 cut(s) 82
SmiMI CAYNNNNRTG 2 cut(s) 119, 168
SmlI CTYRAG 1 cut(s) 205
SmoI CTYRAG 1 cut(s) 205
Sse9I AATT 1 cut(s) 420
SsiI CCGC 1 cut(s) 404
SspMI CTAG 1 cut(s) 261
SstI GAGCTC 1 cut(s) 199
StyD4I CCNGG 1 cut(s) 321
StyI CCWWGG 1 cut(s) 80
TaaI ACNGT 2 cut(s) 157, 391
TaiI ACGT 1 cut(s) 191
TaqI TCGA 3 cut(s) 90, 126, 219
TasI AATT 1 cut(s) 420
TfiI GAWTC 1 cut(s) 59
Tru1I TTAA 4 cut(s) 119, 135, 180, 206
Tru9I TTAA 4 cut(s) 119, 135, 180, 206
TseI GCWGC 1 cut(s) 357
TspDTI ATGAA 1 cut(s) 323
Vha464I CTTAAG 1 cut(s) 205
VspI ATTAAT 1 cut(s) 119
XceI RCATGY 1 cut(s) 30
XspI CTAG 1 cut(s) 261
ZraI GACGTC 1 cut(s) 189
Zsp2I ATGCAT 1 cut(s) 444
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.