MD16G1150000.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0000921
Physical Location & Seq
Forward (+)
11834447 .. 11834854
408 bp
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UTR
Exon/CDS
Intron
MD16G1150000.v1.1.491

Sequence Viewer

Length: 408 bp
ATGAAGAGTTCAACCTCTCCAACACTCATATTTCTCATTCAAATTGTTTGTCTAGTTGTCTTTCTTCCAACAAGCCATTGTCGATCGGTTTTCTTCCCAAGGGATGATGCCAATCTGATCGACCAAACATGCCAACAAACACCAAATTACGATCTCTGCGTCTCTTCTCTTCAATCAGACCCTCGAAGAGCCGACGCAGATGTCAAGGGCTTAGGCATCATAATGGTTGATGTAGTTAAGGCTAGGGTAACTGAAACCCTAAACCTAATCGAGCTGCTTAAGAAGAGCCCGGGAGCTGAAGCCTTAAGGTCTTGCAACAGAATCTACAATGCAGTTTTAGGAACTGATATCCTAGTAGCCAATCAAGCTTTTGTTAAAGGTGACTATGACATAGCAGGCAAGTCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

136

Amino Acids

14.78

Weight (kDa)

6.54

Isoelectric Point (pI)

43.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 38 - 113 2.9e-14 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 200
AcuI CTGAAG 1 cut(s) 318
AflII CTTAAG 2 cut(s) 278, 304
AgsI TTSAA 3 cut(s) 12, 41, 173
AluBI AGCT 3 cut(s) 274, 296, 368
AluI AGCT 3 cut(s) 274, 296, 368
Alw26I GTCTC 1 cut(s) 166
Ama87I CYCGRG 1 cut(s) 289
ApeKI GCWGC 1 cut(s) 274
ArsI GACNNNNNNTTYG 2 cut(s) 34, 66
AsuC2I CCSGG 2 cut(s) 290, 291
AsuHPI GGTGA 1 cut(s) 392
AvaI CYCGRG 1 cut(s) 289
BanII GRGCYC 1 cut(s) 290
BbvI GCAGC 1 cut(s) 261
BcnI CCSGG 2 cut(s) 290, 291
BcoDI GTCTC 1 cut(s) 166
BfaI CTAG 3 cut(s) 53, 243, 353
BfrI CTTAAG 2 cut(s) 278, 304
BisI GCNGC 1 cut(s) 275
BlsI GCNGC 1 cut(s) 276
Bme1390I CCNGG 2 cut(s) 290, 291
BmeT110I CYCGRG 1 cut(s) 289
BmrFI CCNGG 2 cut(s) 290, 291
BmsI GCATC 2 cut(s) 97, 225
Bpu10I CCTNAGC 1 cut(s) 211
BpuMI CCSGG 2 cut(s) 290, 291
BsaBI GATNNNNATC 1 cut(s) 111
BsaJI CCNNGG 2 cut(s) 98, 289
Bse8I GATNNNNATC 1 cut(s) 111
BseDI CCNNGG 2 cut(s) 98, 289
BseGI GGATG 1 cut(s) 109
BseJI GATNNNNATC 1 cut(s) 111
BseXI GCAGC 1 cut(s) 261
Bsh1285I CGRYCG 1 cut(s) 86
BsiEI CGRYCG 1 cut(s) 86
BsiHKCI CYCGRG 1 cut(s) 289
BsiSI CCGG 1 cut(s) 290
BsmAI GTCTC 1 cut(s) 166
BsmBI CGTCTC 1 cut(s) 166
BsoBI CYCGRG 1 cut(s) 289
Bsp1286I GDGCHC 1 cut(s) 290
Bsp143I GATC 3 cut(s) 83, 117, 151
BspQI GCTCTTC 2 cut(s) 181, 278
BspTI CTTAAG 2 cut(s) 278, 304
BssECI CCNNGG 2 cut(s) 98, 289
BssMI GATC 3 cut(s) 83, 117, 151
BssT1I CCWWGG 1 cut(s) 98
Bst6I CTCTTC 4 cut(s) 169, 174, 181, 278
BstAFI CTTAAG 2 cut(s) 278, 304
BstC8I GCNNGC 1 cut(s) 397
BstDEI CTNAG 1 cut(s) 211
BstF5I GGATG 1 cut(s) 109
BstKTI GATC 3 cut(s) 86, 120, 154
BstMAI GTCTC 1 cut(s) 166
BstMBI GATC 3 cut(s) 83, 117, 151
BstMCI CGRYCG 1 cut(s) 86
BstMWI GCNNNNNNNGC 1 cut(s) 365
BstNSI RCATGY 1 cut(s) 132
BstSCI CCNGG 2 cut(s) 288, 289
BstV1I GCAGC 1 cut(s) 261
BtsCI GGATG 1 cut(s) 109
Cac8I GCNNGC 1 cut(s) 397
Cfr9I CCCGGG 1 cut(s) 289
CseI GACGC 2 cut(s) 148, 203
CviAII CATG 1 cut(s) 129
DdeI CTNAG 1 cut(s) 211
DpnI GATC 3 cut(s) 85, 119, 153
DpnII GATC 3 cut(s) 83, 117, 151
DrdI GACNNNNNNGTC 1 cut(s) 200
DseDI GACNNNNNNGTC 1 cut(s) 200
Eam1104I CTCTTC 4 cut(s) 169, 174, 181, 278
EarI CTCTTC 4 cut(s) 169, 174, 181, 278
Eco130I CCWWGG 1 cut(s) 98
Eco24I GRGCYC 1 cut(s) 290
Eco32I GATATC 1 cut(s) 349
Eco57I CTGAAG 1 cut(s) 318
Eco88I CYCGRG 1 cut(s) 289
EcoRV GATATC 1 cut(s) 349
EcoT14I CCWWGG 1 cut(s) 98
EcoT38I GRGCYC 1 cut(s) 290
ErhI CCWWGG 1 cut(s) 98
Esp3I CGTCTC 1 cut(s) 166
FaeI CATG 1 cut(s) 132
FaiI YATR 6 cut(s) 29, 130, 221, 387, 392, 406
FatI CATG 1 cut(s) 128
Fnu4HI GCNGC 1 cut(s) 275
FokI GGATG 1 cut(s) 116
FriOI GRGCYC 1 cut(s) 290
Fsp4HI GCNGC 1 cut(s) 275
FspBI CTAG 3 cut(s) 53, 243, 353
GluI GCNGC 1 cut(s) 275
HapII CCGG 1 cut(s) 290
HgaI GACGC 2 cut(s) 148, 203
Hin1II CATG 1 cut(s) 132
HindIII AAGCTT 1 cut(s) 366
HinfI GANTC 1 cut(s) 321
HpaII CCGG 1 cut(s) 290
HphI GGTGA 1 cut(s) 392
Hpy188I TCNGA 2 cut(s) 117, 178
Hpy99I CGWCG 1 cut(s) 197
HpyCH4V TGCA 2 cut(s) 315, 332
HpyF10VI GCNNNNNNNGC 1 cut(s) 365
HpyF3I CTNAG 1 cut(s) 211
Hsp92II CATG 1 cut(s) 132
Kzo9I GATC 3 cut(s) 83, 117, 151
LguI GCTCTTC 2 cut(s) 181, 278
LmnI GCTCC 1 cut(s) 293
LpnPI CCDG 2 cut(s) 303, 381
Lsp1109I GCAGC 1 cut(s) 261
LweI GCATC 2 cut(s) 97, 225
MaeI CTAG 3 cut(s) 53, 243, 353
MaeIII GTNAC 2 cut(s) 247, 380
MalI GATC 3 cut(s) 85, 119, 153
MboI GATC 3 cut(s) 83, 117, 151
MboII GAAGA 7 cut(s) 16, 56, 85, 156, 161, 198, 295
MhlI GDGCHC 1 cut(s) 290
MluCI AATT 2 cut(s) 42, 145
MmeI TCCRAC 2 cut(s) 44, 92
MnlI CCTC 2 cut(s) 25, 192
MseI TTAA 4 cut(s) 237, 279, 305, 375
MslI CAYNNNNRTG 1 cut(s) 221
MspCI CTTAAG 2 cut(s) 278, 304
MspI CCGG 1 cut(s) 290
MspR9I CCNGG 2 cut(s) 290, 291
MwoI GCNNNNNNNGC 1 cut(s) 365
NciI CCSGG 2 cut(s) 290, 291
NdeII GATC 3 cut(s) 83, 117, 151
NlaIII CATG 1 cut(s) 132
NmuCI GTSAC 1 cut(s) 380
NspI RCATGY 1 cut(s) 132
PciSI GCTCTTC 2 cut(s) 181, 278
PcsI WCGNNNNNNNCGW 1 cut(s) 156
PfeI GAWTC 1 cut(s) 321
PkrI GCNGC 1 cut(s) 276
Ple19I CGATCG 1 cut(s) 86
PvuI CGATCG 1 cut(s) 86
RseI CAYNNNNRTG 1 cut(s) 221
SapI GCTCTTC 2 cut(s) 181, 278
SaqAI TTAA 4 cut(s) 237, 279, 305, 375
SatI GCNGC 1 cut(s) 275
Sau3AI GATC 3 cut(s) 83, 117, 151
ScrFI CCNGG 2 cut(s) 290, 291
SduI GDGCHC 1 cut(s) 290
SetI ASST 7 cut(s) 17, 267, 276, 298, 311, 370, 382
SfaNI GCATC 2 cut(s) 97, 225
SmaI CCCGGG 1 cut(s) 291
SmiMI CAYNNNNRTG 1 cut(s) 221
SmlI CTYRAG 2 cut(s) 278, 304
SmoI CTYRAG 2 cut(s) 278, 304
Sse9I AATT 2 cut(s) 42, 145
SspMI CTAG 3 cut(s) 53, 243, 353
StyD4I CCNGG 2 cut(s) 288, 289
StyI CCWWGG 1 cut(s) 98
TaqI TCGA 4 cut(s) 82, 120, 184, 270
TasI AATT 2 cut(s) 42, 145
TfiI GAWTC 1 cut(s) 321
Tru1I TTAA 4 cut(s) 237, 279, 305, 375
Tru9I TTAA 4 cut(s) 237, 279, 305, 375
TseFI GTSAC 1 cut(s) 380
TseI GCWGC 1 cut(s) 274
Tsp45I GTSAC 1 cut(s) 380
TspDTI ATGAA 1 cut(s) 17
TspMI CCCGGG 1 cut(s) 289
Vha464I CTTAAG 2 cut(s) 278, 304
XceI RCATGY 1 cut(s) 132
XmaI CCCGGG 1 cut(s) 289
XspI CTAG 3 cut(s) 53, 243, 353
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.