Rroxscaffold_5G00344640

Cell wall vacuolar inhibitor of fructosidase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0000921
Physical Location & Seq
Forward (+)
14773750 .. 14777450
3701 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00344640.1

Sequence Viewer

Length: 408 bp
ATGGTCGACGTTGTTAAGCCTAAGGCAACTGATACTCTAAACCATATCAATGACCTTCTTAAACTGACGCCGGGAGCTCAACCGTTAAGTTCTTGCGTCGATGTATATAATGTAGTTATACAGGCTAAAGTCTCAGAAGCCAATGAAGCTCTGTCTAAAGGTGACCCCAAGTTTGCAGAGCAAGGAATGAATGATGTTGGCCTCGAGTCCACTTTTAGGGGAAGTTACGCCAAATTTTTGGTAGAATTTCTTCTAAGGTGGGGCCGCATGGCCACTTTGGATTTCGGGATGAAATTCGAAGCGGGTCCTATCACTTGGTATCCGGTGTGCGAATCAAACCAAGGAGAGGGTGACTCAAACCTCGGTCGATCACAACGGAGTCTTGAAGTCTCGGTGGTCCGAGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

135

Amino Acids

14.8

Weight (kDa)

5.14

Isoelectric Point (pI)

31.92

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 6
AciI CCGC 2 cut(s) 265, 302
AcoI YGGCCR 1 cut(s) 270
AcsI RAATTY 3 cut(s) 233, 245, 293
AcyI GRCGYC 1 cut(s) 68
AfiI CCNNNNNNNGG 2 cut(s) 216, 346
AgsI TTSAA 1 cut(s) 386
AluBI AGCT 2 cut(s) 77, 149
AluI AGCT 2 cut(s) 77, 149
Alw21I GWGCWC 1 cut(s) 79
Alw26I GTCTC 2 cut(s) 136, 394
Ama87I CYCGRG 1 cut(s) 203
AoxI GGCC 3 cut(s) 199, 262, 270
ApoI RAATTY 3 cut(s) 233, 245, 293
ArsI GACNNNNNNTTYG 2 cut(s) 155, 187
Asp700I GAANNNNTTC 1 cut(s) 249
AspS9I GGNCC 3 cut(s) 262, 305, 397
AsuC2I CCSGG 1 cut(s) 72
AsuHPI GGTGA 2 cut(s) 173, 362
AsuII TTCGAA 1 cut(s) 297
AvaI CYCGRG 1 cut(s) 203
AvaII GGWCC 2 cut(s) 305, 397
AxyI CCTNAGG 1 cut(s) 21
BalI TGGCCA 1 cut(s) 272
BanII GRGCYC 1 cut(s) 79
BarI GAAGNNNNNNTAC 2 cut(s) 234, 266
Bbv12I GWGCWC 1 cut(s) 79
BciVI GTATCC 1 cut(s) 330
BcnI CCSGG 1 cut(s) 72
BcoDI GTCTC 2 cut(s) 136, 394
BfuI GTATCC 1 cut(s) 330
BisI GCNGC 1 cut(s) 265
BlsI GCNGC 1 cut(s) 266
Bme1390I CCNGG 1 cut(s) 72
Bme18I GGWCC 2 cut(s) 305, 397
BmeT110I CYCGRG 1 cut(s) 203
BmgT120I GGNCC 3 cut(s) 262, 305, 397
BmiI GGNNCC 2 cut(s) 263, 306
BmrFI CCNGG 1 cut(s) 72
BplI GAGNNNNNCTC 2 cut(s) 338, 370
Bpu14I TTCGAA 1 cut(s) 297
BpuMI CCSGG 1 cut(s) 72
BsaHI GRCGYC 1 cut(s) 68
BsaJI CCNNGG 2 cut(s) 340, 361
BsaWI WCCGGW 1 cut(s) 322
Bsc4I CCNNNNNNNGG 2 cut(s) 216, 346
Bse21I CCTNAGG 1 cut(s) 21
BseDI CCNNGG 2 cut(s) 340, 361
BseGI GGATG 1 cut(s) 294
BseLI CCNNNNNNNGG 2 cut(s) 216, 346
BseMII CTCAG 1 cut(s) 147
Bsh1285I CGRYCG 1 cut(s) 367
BshFI GGCC 3 cut(s) 201, 264, 272
BsiEI CGRYCG 1 cut(s) 367
BsiHKAI GWGCWC 1 cut(s) 79
BsiHKCI CYCGRG 1 cut(s) 203
BsiSI CCGG 2 cut(s) 71, 323
BslI CCNNNNNNNGG 2 cut(s) 216, 346
BsmAI GTCTC 2 cut(s) 136, 394
BsnI GGCC 3 cut(s) 201, 264, 272
BsoBI CYCGRG 1 cut(s) 203
Bsp119I TTCGAA 1 cut(s) 297
Bsp1286I GDGCHC 1 cut(s) 79
Bsp143I GATC 1 cut(s) 368
BspACI CCGC 2 cut(s) 265, 302
BspANI GGCC 3 cut(s) 201, 264, 272
BspCNI CTCAG 1 cut(s) 146
BspLI GGNNCC 2 cut(s) 263, 306
BspT104I TTCGAA 1 cut(s) 297
BssECI CCNNGG 2 cut(s) 340, 361
BssMI GATC 1 cut(s) 368
BssNI GRCGYC 1 cut(s) 68
BssT1I CCWWGG 1 cut(s) 340
Bst4CI ACNGT 1 cut(s) 84
BstACI GRCGYC 1 cut(s) 68
BstBI TTCGAA 1 cut(s) 297
BstDEI CTNAG 3 cut(s) 21, 133, 254
BstEII GGTNACC 1 cut(s) 161
BstF5I GGATG 1 cut(s) 294
BstKTI GATC 1 cut(s) 371
BstMAI GTCTC 2 cut(s) 136, 394
BstMBI GATC 1 cut(s) 368
BstMCI CGRYCG 1 cut(s) 367
BstMWI GCNNNNNNNGC 1 cut(s) 146
BstPI GGTNACC 1 cut(s) 161
BstSCI CCNGG 1 cut(s) 70
BstXI CCANNNNNNTGG 1 cut(s) 238
Bsu36I CCTNAGG 1 cut(s) 21
BsuI GTATCC 1 cut(s) 330
BsuRI GGCC 3 cut(s) 201, 264, 272
BtsCI GGATG 1 cut(s) 294
Cfr13I GGNCC 3 cut(s) 262, 305, 397
CseI GACGC 2 cut(s) 76, 85
CviAII CATG 1 cut(s) 268
CviJI RGCY 8 cut(s) 19, 77, 125, 140, 149, 201, 264, 272
CviKI_1 RGCY 8 cut(s) 19, 77, 125, 140, 149, 201, 264, 272
DdeI CTNAG 3 cut(s) 21, 133, 254
DpnI GATC 1 cut(s) 370
DpnII GATC 1 cut(s) 368
EaeI YGGCCR 1 cut(s) 270
Ecl136II GAGCTC 1 cut(s) 77
Eco130I CCWWGG 1 cut(s) 340
Eco24I GRGCYC 1 cut(s) 79
Eco47I GGWCC 2 cut(s) 305, 397
Eco53kI GAGCTC 1 cut(s) 77
Eco81I CCTNAGG 1 cut(s) 21
Eco88I CYCGRG 1 cut(s) 203
Eco91I GGTNACC 1 cut(s) 161
EcoICRI GAGCTC 1 cut(s) 77
EcoO109I RGGNCCY 1 cut(s) 305
EcoO65I GGTNACC 1 cut(s) 161
EcoT14I CCWWGG 1 cut(s) 340
EcoT38I GRGCYC 1 cut(s) 79
ErhI CCWWGG 1 cut(s) 340
FaeI CATG 1 cut(s) 271
FaiI YATR 6 cut(s) 45, 106, 108, 119, 269, 406
FatI CATG 1 cut(s) 267
FauI CCCGC 1 cut(s) 295
FblI GTMKAC 1 cut(s) 6
Fnu4HI GCNGC 1 cut(s) 265
FokI GGATG 1 cut(s) 301
FriOI GRGCYC 1 cut(s) 79
Fsp4HI GCNGC 1 cut(s) 265
GluI GCNGC 1 cut(s) 265
HaeIII GGCC 3 cut(s) 201, 264, 272
HapII CCGG 2 cut(s) 71, 323
HgaI GACGC 2 cut(s) 76, 85
Hin1I GRCGYC 1 cut(s) 68
Hin1II CATG 1 cut(s) 271
HincII GTYRAC 1 cut(s) 7
HindII GTYRAC 1 cut(s) 7
HinfI GANTC 4 cut(s) 206, 332, 353, 379
HpaII CCGG 2 cut(s) 71, 323
HphI GGTGA 2 cut(s) 173, 362
Hpy166II GTNNAC 2 cut(s) 7, 210
Hpy188I TCNGA 2 cut(s) 136, 401
Hpy188III TCNNGA 2 cut(s) 286, 383
Hpy8I GTNNAC 2 cut(s) 7, 210
Hpy99I CGWCG 2 cut(s) 11, 101
HpyAV CCTTC 1 cut(s) 65
HpyCH4III ACNGT 1 cut(s) 84
HpyCH4IV ACGT 1 cut(s) 9
HpyCH4V TGCA 1 cut(s) 176
HpyF10VI GCNNNNNNNGC 1 cut(s) 146
HpyF3I CTNAG 3 cut(s) 21, 133, 254
HpySE526I ACGT 1 cut(s) 9
Hsp92I GRCGYC 1 cut(s) 68
Hsp92II CATG 1 cut(s) 271
Kzo9I GATC 1 cut(s) 368
LmnI GCTCC 1 cut(s) 74
LpnPI CCDG 3 cut(s) 84, 107, 336
MaeII ACGT 1 cut(s) 9
MaeIII GTNAC 3 cut(s) 161, 224, 350
MalI GATC 1 cut(s) 370
MboI GATC 1 cut(s) 368
MboII GAAGA 1 cut(s) 242
MhlI GDGCHC 1 cut(s) 79
MlsI TGGCCA 1 cut(s) 272
MluCI AATT 3 cut(s) 233, 245, 293
MluNI TGGCCA 1 cut(s) 272
MlyI GAGTC 3 cut(s) 215, 347, 388
MnlI CCTC 3 cut(s) 212, 340, 371
Mox20I TGGCCA 1 cut(s) 272
MroXI GAANNNNTTC 1 cut(s) 249
MscI TGGCCA 1 cut(s) 272
MseI TTAA 3 cut(s) 15, 60, 86
MslI CAYNNNNRTG 1 cut(s) 48
Msp20I TGGCCA 1 cut(s) 272
MspI CCGG 2 cut(s) 71, 323
MspR9I CCNGG 1 cut(s) 72
MwoI GCNNNNNNNGC 1 cut(s) 146
NciI CCSGG 1 cut(s) 72
NdeII GATC 1 cut(s) 368
NlaIII CATG 1 cut(s) 271
NlaIV GGNNCC 2 cut(s) 263, 306
NmuCI GTSAC 2 cut(s) 161, 350
NspV TTCGAA 1 cut(s) 297
PaeR7I CTCGAG 1 cut(s) 203
PdmI GAANNNNTTC 1 cut(s) 249
PfeI GAWTC 1 cut(s) 332
PkrI GCNGC 1 cut(s) 266
PleI GAGTC 3 cut(s) 214, 347, 387
PpsI GAGTC 3 cut(s) 214, 347, 387
PpuMI RGGWCCY 1 cut(s) 305
Psp124BI GAGCTC 1 cut(s) 79
Psp5II RGGWCCY 1 cut(s) 305
PspEI GGTNACC 1 cut(s) 161
PspN4I GGNNCC 2 cut(s) 263, 306
PspPI GGNCC 3 cut(s) 262, 305, 397
PspPPI RGGWCCY 1 cut(s) 305
PspXI VCTCGAGB 1 cut(s) 203
RseI CAYNNNNRTG 1 cut(s) 48
SacI GAGCTC 1 cut(s) 79
SalI GTCGAC 1 cut(s) 5
SaqAI TTAA 3 cut(s) 15, 60, 86
SatI GCNGC 1 cut(s) 265
Sau3AI GATC 1 cut(s) 368
Sau96I GGNCC 3 cut(s) 262, 305, 397
SchI GAGTC 3 cut(s) 215, 347, 388
ScrFI CCNGG 1 cut(s) 72
SduI GDGCHC 1 cut(s) 79
SetI ASST 7 cut(s) 12, 57, 79, 151, 163, 260, 363
Sfr274I CTCGAG 1 cut(s) 203
SfuI TTCGAA 1 cut(s) 297
SinI GGWCC 2 cut(s) 305, 397
SlaI CTCGAG 1 cut(s) 203
SmiMI CAYNNNNRTG 1 cut(s) 48
SmlI CTYRAG 1 cut(s) 203
SmoI CTYRAG 1 cut(s) 203
Sse9I AATT 3 cut(s) 233, 245, 293
SsiI CCGC 2 cut(s) 265, 302
SstI GAGCTC 1 cut(s) 79
StyD4I CCNGG 1 cut(s) 70
StyI CCWWGG 1 cut(s) 340
TaaI ACNGT 1 cut(s) 84
TaiI ACGT 1 cut(s) 12
TaqI TCGA 5 cut(s) 6, 99, 204, 297, 367
TaqII GACCGA 1 cut(s) 353
TasI AATT 3 cut(s) 233, 245, 293
TauI GCSGC 1 cut(s) 267
TfiI GAWTC 1 cut(s) 332
Tru1I TTAA 3 cut(s) 15, 60, 86
Tru9I TTAA 3 cut(s) 15, 60, 86
TseFI GTSAC 2 cut(s) 161, 350
Tsp45I GTSAC 2 cut(s) 161, 350
TspDTI ATGAA 3 cut(s) 159, 203, 305
TspGWI ACGGA 1 cut(s) 391
VpaK11BI GGWCC 2 cut(s) 305, 397
XapI RAATTY 3 cut(s) 233, 245, 293
XhoI CTCGAG 1 cut(s) 203
XmiI GTMKAC 1 cut(s) 6
XmnI GAANNNNTTC 1 cut(s) 249
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.