FvH4_4g20440

Cell wall vacuolar inhibitor of fructosidase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0000921
Physical Location & Seq
Reverse (-)
23710791 .. 23711303
513 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g20440.t1

Sequence Viewer

Length: 513 bp
ATGATGAAGAGAAATTCAATATATGTAGCAGCTCTCGTTCTTGTCATTCAATCTGTGTTTGTTCCAATAAGCCATGGCGATGCCAATCTCATTGAACAAACATGTAGACAAACACCGTATCACGATCTTTGTGTCTCTTCTCTCAAAGCACACCCTCTCAGCGCCAGCGCAGATGTCACAGGCTTGGCTCTCATAATGGTGGATGTTGTGAAGGCCAAGGCAACTGAATGTGCGAACAAGATAAACCATTTGCTTAAGCAAAGGCCTGGAGACCAAGCCTTAAGCTCTTGCAAGAGCAATTACAACGGAGTTTTAGTTGCTGATGTCCCTGTTGCGATTGAAGCTTTAACCAAAGGTGACCCTAAGTTTGCAGAGCAAGGCATGGACGACACCGTCATCGAGGCTAAGTCGTGTGAAGATTACTTTTCAGGGCGTTCTCCTATGACAGATTTAAACAACGCTTCTCAGAAACTTGGAGCTGTGGCTGCAGCAATTGTGAGATTGTTGCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

171

Amino Acids

18.17

Weight (kDa)

6.4

Isoelectric Point (pI)

40.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 29 - 165 1.3e-20 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 392
AccI GTMKAC 1 cut(s) 106
AcsI RAATTY 1 cut(s) 13
AflII CTTAAG 2 cut(s) 254, 280
AflIII ACRYGT 1 cut(s) 101
AgsI TTSAA 4 cut(s) 18, 50, 95, 341
AjnI CCWGG 1 cut(s) 265
AluBI AGCT 4 cut(s) 32, 285, 344, 479
AluI AGCT 4 cut(s) 32, 285, 344, 479
Alw26I GTCTC 2 cut(s) 139, 264
AoxI GGCC 2 cut(s) 213, 263
ApeKI GCWGC 3 cut(s) 29, 485, 488
ApoI RAATTY 1 cut(s) 13
ArsI GACNNNNNNTTYG 2 cut(s) 350, 382
AspLEI GCGC 2 cut(s) 164, 170
AsuHPI GGTGA 1 cut(s) 368
BbvI GCAGC 3 cut(s) 41, 472, 500
BciT130I CCWGG 1 cut(s) 267
BcoDI GTCTC 2 cut(s) 139, 264
BfmI CTRYAG 1 cut(s) 486
BfoI RGCGCY 1 cut(s) 165
BfrI CTTAAG 2 cut(s) 254, 280
BisI GCNGC 3 cut(s) 30, 486, 489
BlsI GCNGC 3 cut(s) 31, 487, 490
Bme1390I CCNGG 1 cut(s) 267
BmrFI CCNGG 1 cut(s) 267
BmsI GCATC 1 cut(s) 70
BpmI CTGGAG 1 cut(s) 288
BsaBI GATNNNNATC 1 cut(s) 84
BsaI GGTCTC 1 cut(s) 264
BsaJI CCNNGG 2 cut(s) 73, 216
BsaXI ACNNNNNCTCC 2 cut(s) 300, 330
Bse8I GATNNNNATC 1 cut(s) 84
BseBI CCWGG 1 cut(s) 267
BseDI CCNNGG 2 cut(s) 73, 216
BseGI GGATG 1 cut(s) 208
BseJI GATNNNNATC 1 cut(s) 84
BseMII CTCAG 2 cut(s) 172, 479
BseXI GCAGC 3 cut(s) 41, 472, 500
BshFI GGCC 2 cut(s) 215, 265
BslFI GGGAC 1 cut(s) 311
BsmAI GTCTC 2 cut(s) 139, 264
BsmFI GGGAC 1 cut(s) 311
BsnI GGCC 2 cut(s) 215, 265
Bso31I GGTCTC 1 cut(s) 264
Bsp143I GATC 1 cut(s) 124
Bsp19I CCATGG 1 cut(s) 73
BspANI GGCC 2 cut(s) 215, 265
BspCNI CTCAG 2 cut(s) 171, 478
BspMAI CTGCAG 1 cut(s) 490
BspTI CTTAAG 2 cut(s) 254, 280
BspTNI GGTCTC 1 cut(s) 264
BssECI CCNNGG 2 cut(s) 73, 216
BssMI GATC 1 cut(s) 124
BssT1I CCWWGG 2 cut(s) 73, 216
Bst2UI CCWGG 1 cut(s) 267
Bst4CI ACNGT 2 cut(s) 117, 394
Bst6I CTCTTC 2 cut(s) 2, 142
BstAFI CTTAAG 2 cut(s) 254, 280
BstC8I GCNNGC 1 cut(s) 166
BstDEI CTNAG 4 cut(s) 158, 363, 405, 465
BstDSI CCRYGG 1 cut(s) 73
BstEII GGTNACC 1 cut(s) 356
BstF5I GGATG 1 cut(s) 208
BstH2I RGCGCY 1 cut(s) 165
BstHHI GCGC 2 cut(s) 164, 170
BstKTI GATC 1 cut(s) 127
BstMAI GTCTC 2 cut(s) 139, 264
BstMBI GATC 1 cut(s) 124
BstMWI GCNNNNNNNGC 2 cut(s) 341, 485
BstNI CCWGG 1 cut(s) 267
BstNSI RCATGY 1 cut(s) 105
BstPI GGTNACC 1 cut(s) 356
BstSCI CCNGG 1 cut(s) 265
BstSFI CTRYAG 1 cut(s) 486
BstV1I GCAGC 3 cut(s) 41, 472, 500
BsuRI GGCC 2 cut(s) 215, 265
BtgI CCRYGG 1 cut(s) 73
BtgZI GCGATG 1 cut(s) 93
BtsCI GGATG 1 cut(s) 208
Cac8I GCNNGC 1 cut(s) 166
CfoI GCGC 2 cut(s) 164, 170
CviAII CATG 3 cut(s) 74, 102, 382
DdeI CTNAG 4 cut(s) 158, 363, 405, 465
DpnI GATC 1 cut(s) 126
DpnII GATC 1 cut(s) 124
DraI TTTAAA 1 cut(s) 453
DrdI GACNNNNNNGTC 1 cut(s) 392
DseDI GACNNNNNNGTC 1 cut(s) 392
Eam1104I CTCTTC 2 cut(s) 2, 142
EarI CTCTTC 2 cut(s) 2, 142
Eco130I CCWWGG 2 cut(s) 73, 216
Eco147I AGGCCT 1 cut(s) 265
Eco31I GGTCTC 1 cut(s) 264
Eco91I GGTNACC 1 cut(s) 356
EcoO65I GGTNACC 1 cut(s) 356
EcoRII CCWGG 1 cut(s) 265
EcoT14I CCWWGG 2 cut(s) 73, 216
ErhI CCWWGG 2 cut(s) 73, 216
FaeI CATG 3 cut(s) 77, 105, 385
FaiI YATR 7 cut(s) 22, 24, 75, 103, 194, 383, 443
FaqI GGGAC 1 cut(s) 311
FatI CATG 3 cut(s) 73, 101, 381
FblI GTMKAC 1 cut(s) 106
Fnu4HI GCNGC 3 cut(s) 30, 486, 489
FokI GGATG 1 cut(s) 215
Fsp4HI GCNGC 3 cut(s) 30, 486, 489
GlaI GCGC 2 cut(s) 163, 169
GluI GCNGC 3 cut(s) 30, 486, 489
GsuI CTGGAG 1 cut(s) 288
HaeII RGCGCY 1 cut(s) 165
HaeIII GGCC 2 cut(s) 215, 265
HhaI GCGC 2 cut(s) 164, 170
Hin1II CATG 3 cut(s) 77, 105, 385
Hin6I GCGC 2 cut(s) 162, 168
HinP1I GCGC 2 cut(s) 162, 168
HindIII AAGCTT 1 cut(s) 342
HphI GGTGA 1 cut(s) 368
Hpy166II GTNNAC 1 cut(s) 107
Hpy188I TCNGA 1 cut(s) 468
Hpy188III TCNNGA 1 cut(s) 122
Hpy8I GTNNAC 1 cut(s) 107
HpyAV CCTTC 1 cut(s) 205
HpyCH4III ACNGT 2 cut(s) 117, 394
HpyCH4V TGCA 3 cut(s) 291, 371, 488
HpyF10VI GCNNNNNNNGC 2 cut(s) 341, 485
HpyF3I CTNAG 4 cut(s) 158, 363, 405, 465
Hsp92II CATG 3 cut(s) 77, 105, 385
HspAI GCGC 2 cut(s) 162, 168
Kzo9I GATC 1 cut(s) 124
LmnI GCTCC 1 cut(s) 476
LpnPI CCDG 6 cut(s) 165, 178, 252, 279, 342, 414
Lsp1109I GCAGC 3 cut(s) 41, 472, 500
LweI GCATC 1 cut(s) 70
MaeIII GTNAC 2 cut(s) 175, 356
MalI GATC 1 cut(s) 126
MboI GATC 1 cut(s) 124
MboII GAAGA 3 cut(s) 19, 129, 428
MfeI CAATTG 1 cut(s) 492
MluCI AATT 3 cut(s) 13, 298, 492
MnlI CCTC 2 cut(s) 165, 394
MseI TTAA 4 cut(s) 255, 281, 347, 452
MslI CAYNNNNRTG 2 cut(s) 78, 197
MspCI CTTAAG 2 cut(s) 254, 280
MspR9I CCNGG 1 cut(s) 267
MunI CAATTG 1 cut(s) 492
MvaI CCWGG 1 cut(s) 267
MwoI GCNNNNNNNGC 2 cut(s) 341, 485
NcoI CCATGG 1 cut(s) 73
NdeII GATC 1 cut(s) 124
NlaIII CATG 3 cut(s) 77, 105, 385
NmuCI GTSAC 2 cut(s) 175, 356
NspI RCATGY 1 cut(s) 105
PceI AGGCCT 1 cut(s) 265
PciI ACATGT 1 cut(s) 101
PflFI GACNNNGTC 1 cut(s) 392
PkrI GCNGC 3 cut(s) 31, 487, 490
PscI ACATGT 1 cut(s) 101
Psp6I CCWGG 1 cut(s) 265
PspEI GGTNACC 1 cut(s) 356
PspGI CCWGG 1 cut(s) 265
PstI CTGCAG 1 cut(s) 490
PsyI GACNNNGTC 1 cut(s) 392
RseI CAYNNNNRTG 2 cut(s) 78, 197
SaqAI TTAA 4 cut(s) 255, 281, 347, 452
SatI GCNGC 3 cut(s) 30, 486, 489
Sau3AI GATC 1 cut(s) 124
ScrFI CCNGG 1 cut(s) 267
SetI ASST 5 cut(s) 34, 287, 346, 358, 481
SfaNI GCATC 1 cut(s) 70
SfcI CTRYAG 1 cut(s) 486
SmiMI CAYNNNNRTG 2 cut(s) 78, 197
SmlI CTYRAG 2 cut(s) 254, 280
SmoI CTYRAG 2 cut(s) 254, 280
Sse9I AATT 3 cut(s) 13, 298, 492
SseBI AGGCCT 1 cut(s) 265
StuI AGGCCT 1 cut(s) 265
StyD4I CCNGG 1 cut(s) 265
StyI CCWWGG 2 cut(s) 73, 216
TaaI ACNGT 2 cut(s) 117, 394
TaqI TCGA 1 cut(s) 399
TasI AATT 3 cut(s) 13, 298, 492
Tru1I TTAA 4 cut(s) 255, 281, 347, 452
Tru9I TTAA 4 cut(s) 255, 281, 347, 452
TseFI GTSAC 2 cut(s) 175, 356
TseI GCWGC 3 cut(s) 29, 485, 488
Tsp45I GTSAC 2 cut(s) 175, 356
TspDTI ATGAA 1 cut(s) 20
TspGWI ACGGA 1 cut(s) 321
Tth111I GACNNNGTC 1 cut(s) 392
Vha464I CTTAAG 2 cut(s) 254, 280
XapI RAATTY 1 cut(s) 13
XceI RCATGY 1 cut(s) 105
XmiI GTMKAC 1 cut(s) 106
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.