Rorug02G0382900

Cell wall vacuolar inhibitor of fructosidase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0000921
Physical Location & Seq
Forward (+)
48837645 .. 48839964
2320 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0382900.1

Sequence Viewer

Length: 744 bp
ATGATTCGCTGCAAAAGAAGAAGAAGAAGAAGAAAAATTTTCAGTAGTGCCCCCGCGATAGCTTTCCGGGACCGCTCCGCCGCTTTCCGTCTACCTTTTCCCCCACTAGGCCCCGCCCTCTCTCTCTCTCTCTCTCTCTCTGAAAACCCATTTGCCATGGCCTCTTCAAGGAAATGGGCAACCACCATATCCTTCGCTGCTTCGTGTATCTATTTCACTGTCATCATCATTCAGATTCCTCTTTTCAGTGTCCCGTGTAGATCTGGAACATGTACATCACCAATAGCAGTCACAGCCCCTCAGCTGATGGCATGTGATCTGTTTCCTTTAGTTGCAATAAAAGCACTAGTCTACCCAGGTGCCATTGCAAATGCATTGGTGAAAAATGAAGCAATTCCAAGCTATAGTAACTTGAAGTTACATGACCCCTCTGCTGTAAAGGCAGCTGATGCAATTGCTGATCTCCAGCGCCTAGAGGTTCTGGTTGGAAGCTACCTGTCAGTCGCTGGAGCATTCTGTGGACTTATAAAACCATGGAGAATGAGCTTCTATGGAGTGCTTCTTATCCTATGGGGCCTTGTCAAGGAAATTATATTGAGCAAATCAGCTAGTCCAAATCTTATATACATCCACCCAGCAATGTCAATTGCCGTCTGTTCTGCCTTCTTGTCTCTAGCTAAGGATGTAAGGAGGATAATTCGAAGTTGCAGAGGTCTTCAGATTGTAAAAGAAAAACGTTTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

247

Amino Acids

27.01

Weight (kDa)

10.24

Isoelectric Point (pI)

61.1

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 527
AccB1I GGYRCC 1 cut(s) 359
AccBSI CCGCTC 1 cut(s) 75
AccI GTMKAC 2 cut(s) 91, 351
AccII CGCG 1 cut(s) 56
AciI CCGC 5 cut(s) 54, 73, 78, 81, 114
AclI AACGTT 1 cut(s) 736
AcsI RAATTY 1 cut(s) 36
AcuI CTGAAG 1 cut(s) 701
AfaI GTAC 1 cut(s) 274
AfiI CCNNNNNNNGG 3 cut(s) 107, 168, 583
AflIII ACRYGT 1 cut(s) 269
AgsI TTSAA 2 cut(s) 168, 415
AhlI ACTAGT 1 cut(s) 346
AjnI CCWGG 1 cut(s) 355
AluBI AGCT 8 cut(s) 62, 304, 402, 446, 492, 546, 608, 677
AluI AGCT 8 cut(s) 62, 304, 402, 446, 492, 546, 608, 677
Alw26I GTCTC 1 cut(s) 675
AlwNI CAGNNNCTG 1 cut(s) 506
AoxI GGCC 3 cut(s) 109, 159, 574
ApeKI GCWGC 3 cut(s) 9, 197, 443
ApoI RAATTY 1 cut(s) 36
Asp700I GAANNNNTTC 1 cut(s) 393
AspLEI GCGC 1 cut(s) 471
AspS9I GGNCC 3 cut(s) 70, 110, 574
AsuC2I CCSGG 1 cut(s) 68
AsuHPI GGTGA 2 cut(s) 270, 391
AsuII TTCGAA 1 cut(s) 700
AvaII GGWCC 1 cut(s) 70
BaeGI GKGCMC 1 cut(s) 52
BanI GGYRCC 1 cut(s) 359
BbsI GAAGAC 1 cut(s) 707
BbvCI CCTCAGC 1 cut(s) 300
BbvI GCAGC 2 cut(s) 184, 455
BccI CCATC 1 cut(s) 301
BceAI ACGGC 1 cut(s) 635
BciT130I CCWGG 1 cut(s) 357
BcnI CCSGG 1 cut(s) 68
BcoDI GTCTC 1 cut(s) 675
BcuI ACTAGT 1 cut(s) 346
BfaI CTAG 5 cut(s) 107, 347, 473, 609, 674
BfmI CTRYAG 1 cut(s) 403
BfoI RGCGCY 1 cut(s) 472
BglII AGATCT 1 cut(s) 260
BisI GCNGC 4 cut(s) 10, 81, 198, 444
BlsI GCNGC 4 cut(s) 11, 82, 199, 445
Bme1390I CCNGG 2 cut(s) 68, 357
Bme18I GGWCC 1 cut(s) 70
BmgT120I GGNCC 3 cut(s) 70, 110, 574
BmiI GGNNCC 4 cut(s) 71, 112, 361, 575
BmrFI CCNGG 2 cut(s) 68, 357
BmsI GCATC 1 cut(s) 439
BpiI GAAGAC 1 cut(s) 707
BpmI CTGGAG 2 cut(s) 449, 528
Bpu10I CCTNAGC 2 cut(s) 300, 678
Bpu14I TTCGAA 1 cut(s) 700
BpuMI CCSGG 1 cut(s) 68
BsaJI CCNNGG 3 cut(s) 156, 355, 533
Bsc4I CCNNNNNNNGG 3 cut(s) 107, 168, 583
Bse3DI GCAATG 2 cut(s) 363, 645
BseBI CCWGG 1 cut(s) 357
BseDI CCNNGG 3 cut(s) 156, 355, 533
BseGI GGATG 2 cut(s) 627, 688
BseLI CCNNNNNNNGG 3 cut(s) 107, 168, 583
BseMI GCAATG 2 cut(s) 363, 645
BseMII CTCAG 1 cut(s) 314
BseSI GKGCMC 1 cut(s) 52
BseXI GCAGC 2 cut(s) 184, 455
BseYI CCCAGC 1 cut(s) 634
Bsh1236I CGCG 1 cut(s) 56
BshFI GGCC 3 cut(s) 111, 161, 576
BshNI GGYRCC 1 cut(s) 359
BsiSI CCGG 1 cut(s) 67
BslFI GGGAC 2 cut(s) 83, 236
BslI CCNNNNNNNGG 3 cut(s) 107, 168, 583
BsmAI GTCTC 1 cut(s) 675
BsmFI GGGAC 2 cut(s) 83, 236
BsmI GAATGC 1 cut(s) 512
BsnI GGCC 3 cut(s) 111, 161, 576
Bsp119I TTCGAA 1 cut(s) 700
Bsp1286I GDGCHC 1 cut(s) 52
Bsp1407I TGTACA 1 cut(s) 272
Bsp143I GATC 3 cut(s) 260, 316, 460
Bsp19I CCATGG 2 cut(s) 156, 533
BspACI CCGC 5 cut(s) 54, 73, 78, 81, 114
BspANI GGCC 3 cut(s) 111, 161, 576
BspCNI CTCAG 1 cut(s) 313
BspFNI CGCG 1 cut(s) 56
BspLI GGNNCC 4 cut(s) 71, 112, 361, 575
BspT104I TTCGAA 1 cut(s) 700
BspT107I GGYRCC 1 cut(s) 359
BsrBI CCGCTC 1 cut(s) 75
BsrDI GCAATG 2 cut(s) 363, 645
BsrGI TGTACA 1 cut(s) 272
BssECI CCNNGG 3 cut(s) 156, 355, 533
BssMI GATC 3 cut(s) 260, 316, 460
BssT1I CCWWGG 2 cut(s) 156, 533
Bst2UI CCWGG 1 cut(s) 357
Bst4CI ACNGT 1 cut(s) 220
Bst6I CTCTTC 1 cut(s) 169
BstAPI GCANNNNNTGC 1 cut(s) 449
BstAUI TGTACA 1 cut(s) 272
BstBI TTCGAA 1 cut(s) 700
BstDEI CTNAG 2 cut(s) 300, 678
BstDSI CCRYGG 2 cut(s) 156, 533
BstENI CCTNNNNNAGG 2 cut(s) 166, 581
BstF5I GGATG 2 cut(s) 627, 688
BstFNI CGCG 1 cut(s) 56
BstH2I RGCGCY 1 cut(s) 472
BstHHI GCGC 1 cut(s) 471
BstKTI GATC 3 cut(s) 263, 319, 463
BstMAI GTCTC 1 cut(s) 675
BstMBI GATC 3 cut(s) 260, 316, 460
BstMWI GCNNNNNNNGC 4 cut(s) 293, 341, 440, 449
BstNI CCWGG 1 cut(s) 357
BstNSI RCATGY 2 cut(s) 273, 315
BstSCI CCNGG 2 cut(s) 66, 355
BstSFI CTRYAG 1 cut(s) 403
BstSLI GKGCMC 1 cut(s) 52
BstUI CGCG 1 cut(s) 56
BstV1I GCAGC 2 cut(s) 184, 455
BstV2I GAAGAC 1 cut(s) 707
BstX2I RGATCY 1 cut(s) 260
BstYI RGATCY 1 cut(s) 260
BsuRI GGCC 3 cut(s) 111, 161, 576
BtgI CCRYGG 2 cut(s) 156, 533
BtsCI GGATG 2 cut(s) 627, 688
BtsIMutI CAGTG 2 cut(s) 216, 253
CaiI CAGNNNCTG 1 cut(s) 506
CfoI GCGC 1 cut(s) 471
Cfr13I GGNCC 3 cut(s) 70, 110, 574
Csp6I GTAC 1 cut(s) 273
CviAII CATG 5 cut(s) 157, 270, 312, 422, 534
CviQI GTAC 1 cut(s) 273
DdeI CTNAG 2 cut(s) 300, 678
DpnI GATC 3 cut(s) 262, 318, 462
DpnII GATC 3 cut(s) 260, 316, 460
Eam1104I CTCTTC 1 cut(s) 169
EarI CTCTTC 1 cut(s) 169
EciI GGCGGA 1 cut(s) 67
Eco130I CCWWGG 2 cut(s) 156, 533
Eco47I GGWCC 1 cut(s) 70
Eco57I CTGAAG 1 cut(s) 701
EcoNI CCTNNNNNAGG 2 cut(s) 166, 581
EcoO109I RGGNCCY 2 cut(s) 110, 574
EcoRII CCWGG 1 cut(s) 355
EcoT14I CCWWGG 2 cut(s) 156, 533
EcoT22I ATGCAT 1 cut(s) 376
ErhI CCWWGG 2 cut(s) 156, 533
FaeI CATG 5 cut(s) 160, 273, 315, 425, 537
FaqI GGGAC 2 cut(s) 83, 236
FatI CATG 5 cut(s) 156, 269, 311, 421, 533
FauI CCCGC 2 cut(s) 61, 121
FblI GTMKAC 2 cut(s) 91, 351
Fnu4HI GCNGC 4 cut(s) 10, 81, 198, 444
FokI GGATG 2 cut(s) 614, 695
Fsp4HI GCNGC 4 cut(s) 10, 81, 198, 444
FspBI CTAG 5 cut(s) 107, 347, 473, 609, 674
GlaI GCGC 1 cut(s) 470
GluI GCNGC 4 cut(s) 10, 81, 198, 444
GsaI CCCAGC 1 cut(s) 638
GsuI CTGGAG 2 cut(s) 449, 528
HaeII RGCGCY 1 cut(s) 472
HaeIII GGCC 3 cut(s) 111, 161, 576
HapII CCGG 1 cut(s) 67
HhaI GCGC 1 cut(s) 471
Hin1II CATG 5 cut(s) 160, 273, 315, 425, 537
Hin6I GCGC 1 cut(s) 469
HinP1I GCGC 1 cut(s) 469
HinfI GANTC 2 cut(s) 4, 235
HpaII CCGG 1 cut(s) 67
HphI GGTGA 2 cut(s) 270, 391
Hpy166II GTNNAC 3 cut(s) 92, 352, 521
Hpy188I TCNGA 3 cut(s) 142, 234, 720
Hpy188III TCNNGA 1 cut(s) 264
Hpy8I GTNNAC 3 cut(s) 92, 352, 521
HpyAV CCTTC 2 cut(s) 202, 673
HpyCH4III ACNGT 1 cut(s) 220
HpyCH4IV ACGT 1 cut(s) 736
HpyCH4V TGCA 6 cut(s) 12, 335, 368, 374, 452, 708
HpyF10VI GCNNNNNNNGC 4 cut(s) 293, 341, 440, 449
HpyF3I CTNAG 2 cut(s) 300, 678
HpySE526I ACGT 1 cut(s) 736
Hsp92II CATG 5 cut(s) 160, 273, 315, 425, 537
HspAI GCGC 1 cut(s) 469
Kzo9I GATC 3 cut(s) 260, 316, 460
LmnI GCTCC 2 cut(s) 80, 509
LpnPI CCDG 9 cut(s) 80, 249, 342, 369, 467, 479, 492, 509, 648
Lsp1109I GCAGC 2 cut(s) 184, 455
LweI GCATC 1 cut(s) 439
MaeI CTAG 5 cut(s) 107, 347, 473, 609, 674
MaeII ACGT 1 cut(s) 736
MaeIII GTNAC 3 cut(s) 289, 407, 417
MalI GATC 3 cut(s) 262, 318, 462
MbiI CCGCTC 1 cut(s) 75
MboI GATC 3 cut(s) 260, 316, 460
MboII GAAGA 7 cut(s) 30, 33, 36, 39, 42, 156, 707
MfeI CAATTG 2 cut(s) 453, 645
MflI RGATCY 1 cut(s) 260
MhlI GDGCHC 1 cut(s) 52
MluCI AATT 6 cut(s) 36, 393, 453, 588, 645, 696
MmeI TCCRAC 1 cut(s) 466
MnlI CCTC 8 cut(s) 128, 172, 249, 309, 439, 469, 684, 704
Mph1103I ATGCAT 1 cut(s) 376
MroXI GAANNNNTTC 1 cut(s) 393
MspA1I CMGCKG 2 cut(s) 304, 446
MspI CCGG 1 cut(s) 67
MspR9I CCNGG 2 cut(s) 68, 357
MunI CAATTG 2 cut(s) 453, 645
Mva1269I GAATGC 1 cut(s) 512
MvaI CCWGG 1 cut(s) 357
MvnI CGCG 1 cut(s) 56
MwoI GCNNNNNNNGC 4 cut(s) 293, 341, 440, 449
NciI CCSGG 1 cut(s) 68
NcoI CCATGG 2 cut(s) 156, 533
NdeII GATC 3 cut(s) 260, 316, 460
NlaIII CATG 5 cut(s) 160, 273, 315, 425, 537
NlaIV GGNNCC 4 cut(s) 71, 112, 361, 575
NmuCI GTSAC 1 cut(s) 289
NsiI ATGCAT 1 cut(s) 376
NspI RCATGY 2 cut(s) 273, 315
NspV TTCGAA 1 cut(s) 700
PciI ACATGT 1 cut(s) 269
PctI GAATGC 1 cut(s) 512
PdmI GAANNNNTTC 1 cut(s) 393
PfeI GAWTC 2 cut(s) 4, 235
PfoI TCCNGGA 1 cut(s) 66
PkrI GCNGC 4 cut(s) 11, 82, 199, 445
PscI ACATGT 1 cut(s) 269
PsiI TTATAA 1 cut(s) 527
Psp1406I AACGTT 1 cut(s) 736
Psp6I CCWGG 1 cut(s) 355
PspFI CCCAGC 1 cut(s) 634
PspGI CCWGG 1 cut(s) 355
PspN4I GGNNCC 4 cut(s) 71, 112, 361, 575
PspPI GGNCC 3 cut(s) 70, 110, 574
PstNI CAGNNNCTG 1 cut(s) 506
PsuI RGATCY 1 cut(s) 260
PvuII CAGCTG 2 cut(s) 304, 446
RsaI GTAC 1 cut(s) 274
RsaNI GTAC 1 cut(s) 273
SatI GCNGC 4 cut(s) 10, 81, 198, 444
Sau3AI GATC 3 cut(s) 260, 316, 460
Sau96I GGNCC 3 cut(s) 70, 110, 574
ScrFI CCNGG 2 cut(s) 68, 357
SduI GDGCHC 1 cut(s) 52
SfaNI GCATC 1 cut(s) 439
SfcI CTRYAG 1 cut(s) 403
SfuI TTCGAA 1 cut(s) 700
SinI GGWCC 1 cut(s) 70
SpeI ACTAGT 1 cut(s) 346
Sse9I AATT 6 cut(s) 36, 393, 453, 588, 645, 696
SsiI CCGC 5 cut(s) 54, 73, 78, 81, 114
SspMI CTAG 5 cut(s) 107, 347, 473, 609, 674
StyD4I CCNGG 2 cut(s) 66, 355
StyI CCWWGG 2 cut(s) 156, 533
TaaI ACNGT 1 cut(s) 220
TaiI ACGT 1 cut(s) 739
TaqI TCGA 1 cut(s) 700
TasI AATT 6 cut(s) 36, 393, 453, 588, 645, 696
TatI WGTACW 1 cut(s) 272
TauI GCSGC 1 cut(s) 83
TfiI GAWTC 2 cut(s) 4, 235
TscAI CASTG 2 cut(s) 223, 253
TseFI GTSAC 1 cut(s) 289
TseI GCWGC 3 cut(s) 9, 197, 443
Tsp45I GTSAC 1 cut(s) 289
TspDTI ATGAA 1 cut(s) 402
TspGWI ACGGA 1 cut(s) 77
TspRI CASTG 2 cut(s) 223, 253
VpaK11BI GGWCC 1 cut(s) 70
XagI CCTNNNNNAGG 2 cut(s) 166, 581
XapI RAATTY 1 cut(s) 36
XceI RCATGY 2 cut(s) 273, 315
XmiI GTMKAC 2 cut(s) 91, 351
XmnI GAANNNNTTC 1 cut(s) 393
XspI CTAG 5 cut(s) 107, 347, 473, 609, 674
Zsp2I ATGCAT 1 cut(s) 376
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.