Prupe.5G112600_v2.0.a1

Cell wall vacuolar inhibitor of fructosidase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp05
OG0000921
Physical Location & Seq
Forward (+)
11636050 .. 11636571
522 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.5G112600.1

Sequence Viewer

Length: 522 bp
ATGAACAATTTAATCTCTCTAGCGTTCATATTTCTCATTCAAGTTTCCCTACTTCCAGCAAGCCAATGCAGGGTTTACTTGCCAATGGATGCCAGTCTCATTGACAAAACCTGCAAACAAACACCCAATTATGATGTTTGTGTCTCTACTCTTAACTCAGACCCTCGAAGCACCGGTGCAGATGTCAAAGGCTTAGGACTCATCATGGTTGATGCAGTTAAGGATAAGGCAACCGGTGCTCTGAATAAAGCCGACGAGCTGCTTAAGCAGAGCCCAGGAGATCAAGCCTTACTCTCTTGCGTCAAAACATACAATGAGGTTTTACAAAATGATGTCCCACAAGGCAGTCAAGCTTTCACTCAAGGTGATTCTAAGTCGGCAGAGCAAGGCATGAATGATATTGCCACGGAAGCAGATTCATGTGAAAGTGGTTTTTCAAGCAGCTCTCCTTTGGTAGATAACAACAAAGCTCTTCTTGATGTTGCGGCTGTGGCTGCAGCAATTGCCAGGACATTGCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

174

Amino Acids

18.21

Weight (kDa)

4.44

Isoelectric Point (pI)

39.28

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 119
AciI CCGC 1 cut(s) 485
AfiI CCNNNNNNNGG 1 cut(s) 70
AflII CTTAAG 1 cut(s) 263
AgeI ACCGGT 2 cut(s) 173, 233
AgsI TTSAA 2 cut(s) 41, 438
AjnI CCWGG 2 cut(s) 274, 506
AluBI AGCT 4 cut(s) 259, 353, 444, 470
AluI AGCT 4 cut(s) 259, 353, 444, 470
Alw21I GWGCWC 1 cut(s) 241
Alw26I GTCTC 2 cut(s) 101, 148
ApeKI GCWGC 4 cut(s) 259, 441, 494, 497
AsiGI ACCGGT 2 cut(s) 173, 233
AsuHPI GGTGA 1 cut(s) 377
BanII GRGCYC 1 cut(s) 275
Bbv12I GWGCWC 1 cut(s) 241
BbvI GCAGC 4 cut(s) 246, 453, 481, 509
BciT130I CCWGG 2 cut(s) 276, 508
BcoDI GTCTC 2 cut(s) 101, 148
BfaI CTAG 1 cut(s) 20
BfmI CTRYAG 1 cut(s) 495
BfrI CTTAAG 1 cut(s) 263
BfuAI ACCTGC 1 cut(s) 119
BisI GCNGC 5 cut(s) 260, 442, 486, 495, 498
BlsI GCNGC 5 cut(s) 261, 443, 487, 496, 499
Bme1390I CCNGG 2 cut(s) 276, 508
BmrFI CCNGG 2 cut(s) 276, 508
BmsI GCATC 2 cut(s) 79, 202
Bpu10I CCTNAGC 1 cut(s) 193
BpuEI CTTGAG 1 cut(s) 345
BsaJI CCNNGG 2 cut(s) 274, 405
BsaWI WCCGGW 2 cut(s) 173, 233
Bsc4I CCNNNNNNNGG 1 cut(s) 70
Bse118I RCCGGY 2 cut(s) 173, 233
Bse1I ACTGG 1 cut(s) 93
Bse3DI GCAATG 1 cut(s) 512
BseBI CCWGG 2 cut(s) 276, 508
BseDI CCNNGG 2 cut(s) 274, 405
BseGI GGATG 1 cut(s) 94
BseLI CCNNNNNNNGG 1 cut(s) 70
BseMI GCAATG 1 cut(s) 512
BseMII CTCAG 1 cut(s) 171
BseNI ACTGG 1 cut(s) 93
BseXI GCAGC 4 cut(s) 246, 453, 481, 509
BsgI GTGCAG 1 cut(s) 198
BshTI ACCGGT 2 cut(s) 173, 233
BsiHKAI GWGCWC 1 cut(s) 241
BsiSI CCGG 2 cut(s) 174, 234
BslFI GGGAC 1 cut(s) 320
BslI CCNNNNNNNGG 1 cut(s) 70
BsmAI GTCTC 2 cut(s) 101, 148
BsmFI GGGAC 1 cut(s) 320
Bsp1286I GDGCHC 2 cut(s) 241, 275
Bsp143I GATC 1 cut(s) 280
BspACI CCGC 1 cut(s) 485
BspCNI CTCAG 1 cut(s) 170
BspMAI CTGCAG 1 cut(s) 499
BspMI ACCTGC 1 cut(s) 119
BspQI GCTCTTC 1 cut(s) 477
BspTI CTTAAG 1 cut(s) 263
BsrDI GCAATG 1 cut(s) 512
BsrFI RCCGGY 2 cut(s) 173, 233
BsrI ACTGG 1 cut(s) 93
BssAI RCCGGY 2 cut(s) 173, 233
BssECI CCNNGG 2 cut(s) 274, 405
BssMI GATC 1 cut(s) 280
Bst2UI CCWGG 2 cut(s) 276, 508
Bst6I CTCTTC 1 cut(s) 477
BstAFI CTTAAG 1 cut(s) 263
BstAPI GCANNNNNTGC 2 cut(s) 236, 503
BstC8I GCNNGC 1 cut(s) 61
BstDEI CTNAG 3 cut(s) 157, 193, 372
BstDSI CCRYGG 1 cut(s) 405
BstF5I GGATG 1 cut(s) 94
BstKTI GATC 1 cut(s) 283
BstMAI GTCTC 2 cut(s) 101, 148
BstMBI GATC 1 cut(s) 280
BstMWI GCNNNNNNNGC 6 cut(s) 236, 265, 410, 491, 494, 503
BstNI CCWGG 2 cut(s) 276, 508
BstSCI CCNGG 2 cut(s) 274, 506
BstSFI CTRYAG 1 cut(s) 495
BstV1I GCAGC 4 cut(s) 246, 453, 481, 509
BtgI CCRYGG 1 cut(s) 405
BtsCI GGATG 1 cut(s) 94
BveI ACCTGC 1 cut(s) 119
Cac8I GCNNGC 1 cut(s) 61
Cfr10I RCCGGY 2 cut(s) 173, 233
CseI GACGC 1 cut(s) 289
CspAI ACCGGT 2 cut(s) 173, 233
CviAII CATG 3 cut(s) 205, 391, 420
DdeI CTNAG 3 cut(s) 157, 193, 372
DpnI GATC 1 cut(s) 282
DpnII GATC 1 cut(s) 280
Eam1104I CTCTTC 1 cut(s) 477
EarI CTCTTC 1 cut(s) 477
Eco24I GRGCYC 1 cut(s) 275
EcoRII CCWGG 2 cut(s) 274, 506
EcoT38I GRGCYC 1 cut(s) 275
FaeI CATG 3 cut(s) 208, 394, 423
FaiI YATR 6 cut(s) 29, 132, 206, 310, 392, 421
FalI AAGNNNNNCTT 2 cut(s) 459, 491
FaqI GGGAC 1 cut(s) 320
FatI CATG 3 cut(s) 204, 390, 419
Fnu4HI GCNGC 5 cut(s) 260, 442, 486, 495, 498
FokI GGATG 1 cut(s) 101
FriOI GRGCYC 1 cut(s) 275
Fsp4HI GCNGC 5 cut(s) 260, 442, 486, 495, 498
FspBI CTAG 1 cut(s) 20
GluI GCNGC 5 cut(s) 260, 442, 486, 495, 498
HapII CCGG 2 cut(s) 174, 234
HgaI GACGC 1 cut(s) 289
Hin1II CATG 3 cut(s) 208, 394, 423
HindIII AAGCTT 1 cut(s) 351
HinfI GANTC 3 cut(s) 198, 368, 416
HpaII CCGG 2 cut(s) 174, 234
HphI GGTGA 1 cut(s) 377
Hpy166II GTNNAC 1 cut(s) 76
Hpy188I TCNGA 2 cut(s) 160, 243
Hpy188III TCNNGA 1 cut(s) 476
Hpy8I GTNNAC 1 cut(s) 76
Hpy99I CGWCG 1 cut(s) 257
HpyCH4V TGCA 5 cut(s) 69, 114, 179, 215, 497
HpyF10VI GCNNNNNNNGC 6 cut(s) 236, 265, 410, 491, 494, 503
HpyF3I CTNAG 3 cut(s) 157, 193, 372
Hsp92II CATG 3 cut(s) 208, 394, 423
Kzo9I GATC 1 cut(s) 280
LguI GCTCTTC 1 cut(s) 477
LpnPI CCDG 9 cut(s) 55, 69, 106, 124, 187, 247, 261, 288, 493
Lsp1109I GCAGC 4 cut(s) 246, 453, 481, 509
LweI GCATC 2 cut(s) 79, 202
MaeI CTAG 1 cut(s) 20
MalI GATC 1 cut(s) 282
MboI GATC 1 cut(s) 280
MboII GAAGA 1 cut(s) 464
MfeI CAATTG 1 cut(s) 501
MhlI GDGCHC 2 cut(s) 241, 275
MluCI AATT 3 cut(s) 7, 127, 501
MlyI GAGTC 1 cut(s) 192
MnlI CCTC 2 cut(s) 174, 310
MseI TTAA 4 cut(s) 11, 153, 219, 264
MspCI CTTAAG 1 cut(s) 263
MspI CCGG 2 cut(s) 174, 234
MspR9I CCNGG 2 cut(s) 276, 508
MunI CAATTG 1 cut(s) 501
MvaI CCWGG 2 cut(s) 276, 508
MwoI GCNNNNNNNGC 6 cut(s) 236, 265, 410, 491, 494, 503
NdeII GATC 1 cut(s) 280
NlaIII CATG 3 cut(s) 208, 394, 423
PciSI GCTCTTC 1 cut(s) 477
PfeI GAWTC 2 cut(s) 368, 416
PinAI ACCGGT 2 cut(s) 173, 233
PkrI GCNGC 5 cut(s) 261, 443, 487, 496, 499
PleI GAGTC 1 cut(s) 192
PpsI GAGTC 1 cut(s) 192
Psp6I CCWGG 2 cut(s) 274, 506
PspGI CCWGG 2 cut(s) 274, 506
PstI CTGCAG 1 cut(s) 499
SapI GCTCTTC 1 cut(s) 477
SaqAI TTAA 4 cut(s) 11, 153, 219, 264
SatI GCNGC 5 cut(s) 260, 442, 486, 495, 498
Sau3AI GATC 1 cut(s) 280
SchI GAGTC 1 cut(s) 192
ScrFI CCNGG 2 cut(s) 276, 508
SduI GDGCHC 2 cut(s) 241, 275
SetI ASST 7 cut(s) 113, 261, 321, 355, 367, 446, 472
SfaNI GCATC 2 cut(s) 79, 202
SfcI CTRYAG 1 cut(s) 495
SgrAI CRCCGGYG 1 cut(s) 173
SmlI CTYRAG 2 cut(s) 263, 360
SmoI CTYRAG 2 cut(s) 263, 360
Sse9I AATT 3 cut(s) 7, 127, 501
SsiI CCGC 1 cut(s) 485
SspMI CTAG 1 cut(s) 20
StyD4I CCNGG 2 cut(s) 274, 506
TaqI TCGA 1 cut(s) 166
TasI AATT 3 cut(s) 7, 127, 501
TauI GCSGC 1 cut(s) 488
TfiI GAWTC 2 cut(s) 368, 416
Tru1I TTAA 4 cut(s) 11, 153, 219, 264
Tru9I TTAA 4 cut(s) 11, 153, 219, 264
TseI GCWGC 4 cut(s) 259, 441, 494, 497
TspDTI ATGAA 4 cut(s) 16, 17, 407, 408
TspGWI ACGGA 1 cut(s) 422
Vha464I CTTAAG 1 cut(s) 263
XspI CTAG 1 cut(s) 20
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.