MD13G1150400.v1.1

Cell wall vacuolar inhibitor of fructosidase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0000921
Physical Location & Seq
Reverse (-)
11785576 .. 11786106
531 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1150400.v1.1.491

Sequence Viewer

Length: 531 bp
ATGAACAACTCAACATGTCACATTCTACTAGTTTGTCTAATTCTAGTCTTTCTTCCACTAAGCCATTGCAGCAGGGCTGCGAAGATCTTGCAAATGGAAGTTGATCTCATCGACCAAACCTGCAAAGTAACACCAAACTACGATCTCTGCGTCTCCTCTCTCGAATCAGACCCTCGAAGCGCGTACGCCGACGTTGCAGGCTTAGGCCTCATTATAGTTAACGTAGCCACTGTTAAGGCAACAGATGTTGTGAACAAAATCGAGGAGCTGCTTAAGCAGAACCCTGAAGAAAAACCCTTAAAGTATTGTACTCGCTATTACCGTAAAGTTTTGGAAGCTGACATCCCGGAAGCCAGTCAAGGTTTTCGTTCGGGTAACCCTAAGTTTGCAGAGGAAGGCATGATCGGCGCCACTGGCGAGGCTGAATCATGTCAGAGTAGTTTTTCTGGCAAGTCTCCTTTGACAGATATGAACAAAGGTTTCCAGGATGTTTCGAATATAGCTGCAGCAATTGCTAGAATTTTGATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

177

Amino Acids

19.16

Weight (kDa)

5.22

Isoelectric Point (pI)

51.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 35 - 171 2.1e-18 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 128
AccB1I GGYRCC 1 cut(s) 407
AccII CGCG 1 cut(s) 182
AcsI RAATTY 1 cut(s) 519
AcuI CTGAAG 1 cut(s) 306
AcyI GRCGYC 1 cut(s) 408
AfaI GTAC 2 cut(s) 185, 310
AflII CTTAAG 1 cut(s) 272
AflIII ACRYGT 1 cut(s) 14
AhlI ACTAGT 1 cut(s) 28
AjnI CCWGG 1 cut(s) 483
AluBI AGCT 3 cut(s) 268, 338, 503
AluI AGCT 3 cut(s) 268, 338, 503
Alw26I GTCTC 2 cut(s) 157, 459
AoxI GGCC 1 cut(s) 205
ApeKI GCWGC 5 cut(s) 69, 77, 268, 503, 506
ApoI RAATTY 1 cut(s) 519
AspLEI GCGC 2 cut(s) 182, 410
AsuC2I CCSGG 1 cut(s) 347
AsuII TTCGAA 1 cut(s) 494
BanI GGYRCC 1 cut(s) 407
BbvI GCAGC 5 cut(s) 64, 81, 255, 490, 518
BcgI CGANNNNNNTGC 2 cut(s) 70, 104
BciT130I CCWGG 1 cut(s) 485
BcnI CCSGG 1 cut(s) 347
BcoDI GTCTC 2 cut(s) 157, 459
BcuI ACTAGT 1 cut(s) 28
BfaI CTAG 3 cut(s) 29, 44, 516
BfmI CTRYAG 1 cut(s) 504
BfoI RGCGCY 1 cut(s) 411
BfrI CTTAAG 1 cut(s) 272
BfuAI ACCTGC 1 cut(s) 128
BglII AGATCT 1 cut(s) 84
BisI GCNGC 5 cut(s) 70, 78, 269, 504, 507
BlsI GCNGC 5 cut(s) 71, 79, 270, 505, 508
Bme1390I CCNGG 2 cut(s) 347, 485
BmiI GGNNCC 1 cut(s) 409
BmrFI CCNGG 2 cut(s) 347, 485
Bpu10I CCTNAGC 1 cut(s) 202
Bpu14I TTCGAA 1 cut(s) 494
BpuMI CCSGG 1 cut(s) 347
BsaHI GRCGYC 1 cut(s) 408
Bse1I ACTGG 2 cut(s) 354, 418
Bse3DI GCAATG 1 cut(s) 64
BseBI CCWGG 1 cut(s) 485
BseGI GGATG 2 cut(s) 342, 493
BseMI GCAATG 1 cut(s) 64
BseNI ACTGG 2 cut(s) 354, 418
BseRI GAGGAG 2 cut(s) 145, 278
BseXI GCAGC 5 cut(s) 64, 81, 255, 490, 518
Bsh1236I CGCG 1 cut(s) 182
BshFI GGCC 1 cut(s) 207
BshNI GGYRCC 1 cut(s) 407
BsiSI CCGG 1 cut(s) 347
BsiWI CGTACG 1 cut(s) 183
BsmAI GTCTC 2 cut(s) 157, 459
BsmBI CGTCTC 1 cut(s) 157
BsnI GGCC 1 cut(s) 207
Bsp119I TTCGAA 1 cut(s) 494
Bsp143I GATC 4 cut(s) 84, 103, 142, 402
BspANI GGCC 1 cut(s) 207
BspFNI CGCG 1 cut(s) 182
BspLI GGNNCC 1 cut(s) 409
BspMAI CTGCAG 1 cut(s) 508
BspMI ACCTGC 1 cut(s) 128
BspT104I TTCGAA 1 cut(s) 494
BspT107I GGYRCC 1 cut(s) 407
BspTI CTTAAG 1 cut(s) 272
BsrDI GCAATG 1 cut(s) 64
BsrI ACTGG 2 cut(s) 354, 418
BssMI GATC 4 cut(s) 84, 103, 142, 402
BssNI GRCGYC 1 cut(s) 408
Bst2UI CCWGG 1 cut(s) 485
Bst4CI ACNGT 2 cut(s) 232, 323
BstACI GRCGYC 1 cut(s) 408
BstAFI CTTAAG 1 cut(s) 272
BstAPI GCANNNNNTGC 1 cut(s) 512
BstBI TTCGAA 1 cut(s) 494
BstC8I GCNNGC 1 cut(s) 199
BstDEI CTNAG 3 cut(s) 59, 202, 381
BstEII GGTNACC 1 cut(s) 374
BstF5I GGATG 2 cut(s) 342, 493
BstFNI CGCG 1 cut(s) 182
BstH2I RGCGCY 1 cut(s) 411
BstHHI GCGC 2 cut(s) 182, 410
BstKTI GATC 4 cut(s) 87, 106, 145, 405
BstMAI GTCTC 2 cut(s) 157, 459
BstMBI GATC 4 cut(s) 84, 103, 142, 402
BstMWI GCNNNNNNNGC 6 cut(s) 69, 194, 274, 405, 414, 512
BstNI CCWGG 1 cut(s) 485
BstNSI RCATGY 1 cut(s) 18
BstPI GGTNACC 1 cut(s) 374
BstSCI CCNGG 2 cut(s) 345, 483
BstSFI CTRYAG 1 cut(s) 504
BstUI CGCG 1 cut(s) 182
BstV1I GCAGC 5 cut(s) 64, 81, 255, 490, 518
BstX2I RGATCY 1 cut(s) 84
BstYI RGATCY 1 cut(s) 84
BsuRI GGCC 1 cut(s) 207
BtsCI GGATG 2 cut(s) 342, 493
BtsIMutI CAGTG 2 cut(s) 228, 411
BveI ACCTGC 1 cut(s) 128
Cac8I GCNNGC 1 cut(s) 199
CfoI GCGC 2 cut(s) 182, 410
CseI GACGC 1 cut(s) 139
Csp6I GTAC 2 cut(s) 184, 309
CviAII CATG 3 cut(s) 15, 400, 429
CviQI GTAC 2 cut(s) 184, 309
DdeI CTNAG 3 cut(s) 59, 202, 381
DinI GGCGCC 1 cut(s) 409
DpnI GATC 4 cut(s) 86, 105, 144, 404
DpnII GATC 4 cut(s) 84, 103, 142, 402
Eco147I AGGCCT 1 cut(s) 207
Eco57I CTGAAG 1 cut(s) 306
Eco91I GGTNACC 1 cut(s) 374
EcoO65I GGTNACC 1 cut(s) 374
EcoRII CCWGG 1 cut(s) 483
EgeI GGCGCC 1 cut(s) 409
EheI GGCGCC 1 cut(s) 409
Esp3I CGTCTC 1 cut(s) 157
FaeI CATG 3 cut(s) 18, 403, 432
FaiI YATR 6 cut(s) 16, 215, 401, 430, 470, 500
FatI CATG 3 cut(s) 14, 399, 428
Fnu4HI GCNGC 5 cut(s) 70, 78, 269, 504, 507
FokI GGATG 2 cut(s) 329, 500
Fsp4HI GCNGC 5 cut(s) 70, 78, 269, 504, 507
FspBI CTAG 3 cut(s) 29, 44, 516
GlaI GCGC 2 cut(s) 181, 409
GluI GCNGC 5 cut(s) 70, 78, 269, 504, 507
HaeII RGCGCY 1 cut(s) 411
HaeIII GGCC 1 cut(s) 207
HapII CCGG 1 cut(s) 347
HgaI GACGC 1 cut(s) 139
HhaI GCGC 2 cut(s) 182, 410
Hin1I GRCGYC 1 cut(s) 408
Hin1II CATG 3 cut(s) 18, 403, 432
Hin6I GCGC 2 cut(s) 180, 408
HinP1I GCGC 2 cut(s) 180, 408
HincII GTYRAC 1 cut(s) 220
HindII GTYRAC 1 cut(s) 220
HinfI GANTC 2 cut(s) 164, 425
HpaI GTTAAC 1 cut(s) 220
HpaII CCGG 1 cut(s) 347
Hpy166II GTNNAC 2 cut(s) 220, 253
Hpy188I TCNGA 2 cut(s) 169, 435
Hpy188III TCNNGA 1 cut(s) 161
Hpy8I GTNNAC 2 cut(s) 220, 253
Hpy99I CGWCG 1 cut(s) 194
HpyAV CCTTC 1 cut(s) 389
HpyCH4III ACNGT 2 cut(s) 232, 323
HpyCH4IV ACGT 2 cut(s) 192, 222
HpyCH4V TGCA 6 cut(s) 69, 91, 123, 197, 389, 506
HpyF10VI GCNNNNNNNGC 6 cut(s) 69, 194, 274, 405, 414, 512
HpyF3I CTNAG 3 cut(s) 59, 202, 381
HpySE526I ACGT 2 cut(s) 192, 222
Hsp92I GRCGYC 1 cut(s) 408
Hsp92II CATG 3 cut(s) 18, 403, 432
HspAI GCGC 2 cut(s) 180, 408
KasI GGCGCC 1 cut(s) 407
KspAI GTTAAC 1 cut(s) 220
Kzo9I GATC 4 cut(s) 84, 103, 142, 402
LmnI GCTCC 1 cut(s) 265
Lsp1109I GCAGC 5 cut(s) 64, 81, 255, 490, 518
MaeI CTAG 3 cut(s) 29, 44, 516
MaeII ACGT 2 cut(s) 192, 222
MaeIII GTNAC 3 cut(s) 17, 127, 374
MalI GATC 4 cut(s) 86, 105, 144, 404
MboI GATC 4 cut(s) 84, 103, 142, 402
MboII GAAGA 3 cut(s) 44, 94, 299
MfeI CAATTG 1 cut(s) 510
MflI RGATCY 1 cut(s) 84
MluCI AATT 3 cut(s) 39, 510, 519
Mly113I GGCGCC 1 cut(s) 408
MnlI CCTC 6 cut(s) 166, 183, 218, 256, 385, 412
MseI TTAA 5 cut(s) 219, 234, 273, 299, 529
MspCI CTTAAG 1 cut(s) 272
MspI CCGG 1 cut(s) 347
MspR9I CCNGG 2 cut(s) 347, 485
MunI CAATTG 1 cut(s) 510
MvaI CCWGG 1 cut(s) 485
MvnI CGCG 1 cut(s) 182
MwoI GCNNNNNNNGC 6 cut(s) 69, 194, 274, 405, 414, 512
NarI GGCGCC 1 cut(s) 408
NciI CCSGG 1 cut(s) 347
NdeII GATC 4 cut(s) 84, 103, 142, 402
NlaIII CATG 3 cut(s) 18, 403, 432
NlaIV GGNNCC 1 cut(s) 409
NmuCI GTSAC 1 cut(s) 17
NspI RCATGY 1 cut(s) 18
NspV TTCGAA 1 cut(s) 494
PceI AGGCCT 1 cut(s) 207
PciI ACATGT 1 cut(s) 14
PcsI WCGNNNNNNNCGW 2 cut(s) 147, 319
PfeI GAWTC 2 cut(s) 164, 425
Pfl23II CGTACG 1 cut(s) 183
PfoI TCCNGGA 2 cut(s) 345, 483
PkrI GCNGC 5 cut(s) 71, 79, 270, 505, 508
PluTI GGCGCC 1 cut(s) 411
PscI ACATGT 1 cut(s) 14
Psp6I CCWGG 1 cut(s) 483
PspEI GGTNACC 1 cut(s) 374
PspGI CCWGG 1 cut(s) 483
PspLI CGTACG 1 cut(s) 183
PspN4I GGNNCC 1 cut(s) 409
PstI CTGCAG 1 cut(s) 508
PsuI RGATCY 1 cut(s) 84
RsaI GTAC 2 cut(s) 185, 310
RsaNI GTAC 2 cut(s) 184, 309
SaqAI TTAA 5 cut(s) 219, 234, 273, 299, 529
SatI GCNGC 5 cut(s) 70, 78, 269, 504, 507
Sau3AI GATC 4 cut(s) 84, 103, 142, 402
ScrFI CCNGG 2 cut(s) 347, 485
SetI ASST 8 cut(s) 122, 195, 225, 270, 340, 364, 481, 505
SfcI CTRYAG 1 cut(s) 504
SfoI GGCGCC 1 cut(s) 409
SfuI TTCGAA 1 cut(s) 494
SmlI CTYRAG 1 cut(s) 272
SmoI CTYRAG 1 cut(s) 272
SpeI ACTAGT 1 cut(s) 28
Sse9I AATT 3 cut(s) 39, 510, 519
SseBI AGGCCT 1 cut(s) 207
SspDI GGCGCC 1 cut(s) 407
SspMI CTAG 3 cut(s) 29, 44, 516
StuI AGGCCT 1 cut(s) 207
StyD4I CCNGG 2 cut(s) 345, 483
TaaI ACNGT 2 cut(s) 232, 323
TaiI ACGT 2 cut(s) 195, 225
TaqI TCGA 5 cut(s) 111, 162, 175, 261, 494
TasI AATT 3 cut(s) 39, 510, 519
TatI WGTACW 1 cut(s) 308
TfiI GAWTC 2 cut(s) 164, 425
Tru1I TTAA 5 cut(s) 219, 234, 273, 299, 529
Tru9I TTAA 5 cut(s) 219, 234, 273, 299, 529
TscAI CASTG 2 cut(s) 235, 418
TseFI GTSAC 1 cut(s) 17
TseI GCWGC 5 cut(s) 69, 77, 268, 503, 506
Tsp45I GTSAC 1 cut(s) 17
TspDTI ATGAA 2 cut(s) 17, 485
TspRI CASTG 2 cut(s) 235, 418
Vha464I CTTAAG 1 cut(s) 272
XapI RAATTY 1 cut(s) 519
XceI RCATGY 1 cut(s) 18
XspI CTAG 3 cut(s) 29, 44, 516
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.