FvH4_1g05890

Cell wall vacuolar inhibitor of fructosidase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0000921
Physical Location & Seq
Reverse (-)
3126482 .. 3127239
758 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g05890.t1

Sequence Viewer

Length: 471 bp
ATGGGTCAATGTAGCATAGATTTTTGGCCAATGGATGCTAAACTCATTGACGAAACATGTCGAAAAACACCAAACCCGAATCTCTGTGTCTCATCTCTCAAATCAGATCCTCGAAGTTCTGGTGCCGATGTCAAAGGCTTGGGCATAATAATGGTGGATGTGGTTAAGGCCAAAGCAATTATCGGTCAAAACAAAGTAAGTGGATTACTTAAGCAGAGCCCAGGAGATCTACGCTTGAAAGTTTGTTCAGGTGACTATGATGCGATTTCAAACCATCAGGTCCCAAATGCTTCCTTGGCATTTAGTAGAGATATCCCTCATATTGCTGAAGAAGACATGAGTGAAGCTGCTGACGAGGCAAGGGCATGCGAGTTAAGTTTTTCAGGGAGGTCTCCTCTAACAGATGTAAATAATGCTGTGGCAGATGTCGCGTCTATTACTGCAGCTATAGCAAAGCAATTAGGCGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

157

Amino Acids

16.43

Weight (kDa)

5.1

Isoelectric Point (pI)

38.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 14 - 150 3.7e-17 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 122
AccII CGCG 1 cut(s) 431
AciI CCGC 1 cut(s) 465
AclWI GGATC 1 cut(s) 101
AcoI YGGCCR 1 cut(s) 26
AcuI CTGAAG 1 cut(s) 348
AflII CTTAAG 1 cut(s) 209
AflIII ACRYGT 1 cut(s) 56
AgsI TTSAA 2 cut(s) 238, 270
AjnI CCWGG 1 cut(s) 220
AluBI AGCT 2 cut(s) 347, 446
AluI AGCT 2 cut(s) 347, 446
Alw26I GTCTC 2 cut(s) 94, 396
AlwI GGATC 1 cut(s) 101
AoxI GGCC 2 cut(s) 26, 168
ApeKI GCWGC 2 cut(s) 347, 443
AspS9I GGNCC 1 cut(s) 280
AsuHPI GGTGA 1 cut(s) 263
AvaII GGWCC 1 cut(s) 280
BalI TGGCCA 1 cut(s) 28
BanI GGYRCC 1 cut(s) 122
BanII GRGCYC 1 cut(s) 221
BbsI GAAGAC 1 cut(s) 339
BbvI GCAGC 2 cut(s) 334, 455
BccI CCATC 1 cut(s) 282
BciT130I CCWGG 1 cut(s) 222
BcoDI GTCTC 2 cut(s) 94, 396
BfmI CTRYAG 2 cut(s) 441, 447
BfrI CTTAAG 1 cut(s) 209
BglII AGATCT 1 cut(s) 226
BisI GCNGC 2 cut(s) 348, 444
BlsI GCNGC 2 cut(s) 349, 445
Bme1390I CCNGG 1 cut(s) 222
Bme18I GGWCC 1 cut(s) 280
BmgT120I GGNCC 1 cut(s) 280
BmiI GGNNCC 2 cut(s) 124, 282
BmrFI CCNGG 1 cut(s) 222
BmsI GCATC 2 cut(s) 25, 250
BpiI GAAGAC 1 cut(s) 339
BplI GAGNNNNNCTC 2 cut(s) 379, 411
BsaI GGTCTC 1 cut(s) 396
BsaJI CCNNGG 2 cut(s) 220, 294
BseBI CCWGG 1 cut(s) 222
BseDI CCNNGG 2 cut(s) 220, 294
BseGI GGATG 2 cut(s) 40, 163
BseRI GAGGAG 1 cut(s) 384
BseXI GCAGC 2 cut(s) 334, 455
Bsh1236I CGCG 1 cut(s) 431
BshFI GGCC 2 cut(s) 28, 170
BshNI GGYRCC 1 cut(s) 122
BslFI GGGAC 1 cut(s) 266
BsmAI GTCTC 2 cut(s) 94, 396
BsmFI GGGAC 1 cut(s) 266
BsnI GGCC 2 cut(s) 28, 170
Bso31I GGTCTC 1 cut(s) 396
Bsp1286I GDGCHC 1 cut(s) 221
Bsp143I GATC 2 cut(s) 106, 226
BspACI CCGC 1 cut(s) 465
BspANI GGCC 2 cut(s) 28, 170
BspFNI CGCG 1 cut(s) 431
BspLI GGNNCC 2 cut(s) 124, 282
BspMAI CTGCAG 1 cut(s) 445
BspPI GGATC 1 cut(s) 101
BspT107I GGYRCC 1 cut(s) 122
BspTI CTTAAG 1 cut(s) 209
BspTNI GGTCTC 1 cut(s) 396
BssECI CCNNGG 2 cut(s) 220, 294
BssMI GATC 2 cut(s) 106, 226
BssT1I CCWWGG 1 cut(s) 294
Bst2UI CCWGG 1 cut(s) 222
BstAFI CTTAAG 1 cut(s) 209
BstC8I GCNNGC 1 cut(s) 367
BstF5I GGATG 2 cut(s) 40, 163
BstFNI CGCG 1 cut(s) 431
BstKTI GATC 2 cut(s) 109, 229
BstMAI GTCTC 2 cut(s) 94, 396
BstMBI GATC 2 cut(s) 106, 226
BstMWI GCNNNNNNNGC 4 cut(s) 296, 356, 428, 449
BstNI CCWGG 1 cut(s) 222
BstNSI RCATGY 2 cut(s) 60, 369
BstSCI CCNGG 1 cut(s) 220
BstSFI CTRYAG 2 cut(s) 441, 447
BstUI CGCG 1 cut(s) 431
BstV1I GCAGC 2 cut(s) 334, 455
BstV2I GAAGAC 1 cut(s) 339
BstX2I RGATCY 2 cut(s) 106, 226
BstYI RGATCY 2 cut(s) 106, 226
BsuRI GGCC 2 cut(s) 28, 170
BtsCI GGATG 2 cut(s) 40, 163
Cac8I GCNNGC 1 cut(s) 367
Cfr13I GGNCC 1 cut(s) 280
CseI GACGC 1 cut(s) 420
CspCI CAANNNNNGTGG 2 cut(s) 181, 216
CviAII CATG 3 cut(s) 57, 337, 366
CviJI RGCY 6 cut(s) 28, 138, 170, 219, 347, 446
CviKI_1 RGCY 6 cut(s) 28, 138, 170, 219, 347, 446
DpnI GATC 2 cut(s) 108, 228
DpnII GATC 2 cut(s) 106, 226
EaeI YGGCCR 1 cut(s) 26
Eco130I CCWWGG 1 cut(s) 294
Eco24I GRGCYC 1 cut(s) 221
Eco31I GGTCTC 1 cut(s) 396
Eco32I GATATC 1 cut(s) 313
Eco47I GGWCC 1 cut(s) 280
Eco57I CTGAAG 1 cut(s) 348
EcoO109I RGGNCCY 1 cut(s) 280
EcoRII CCWGG 1 cut(s) 220
EcoRV GATATC 1 cut(s) 313
EcoT14I CCWWGG 1 cut(s) 294
EcoT38I GRGCYC 1 cut(s) 221
ErhI CCWWGG 1 cut(s) 294
FaeI CATG 3 cut(s) 60, 340, 369
FaiI YATR 8 cut(s) 17, 58, 146, 258, 321, 338, 367, 449
FaqI GGGAC 1 cut(s) 266
FatI CATG 3 cut(s) 56, 336, 365
Fnu4HI GCNGC 2 cut(s) 348, 444
FokI GGATG 2 cut(s) 47, 170
FriOI GRGCYC 1 cut(s) 221
Fsp4HI GCNGC 2 cut(s) 348, 444
GluI GCNGC 2 cut(s) 348, 444
HaeIII GGCC 2 cut(s) 28, 170
HgaI GACGC 1 cut(s) 420
Hin1II CATG 3 cut(s) 60, 340, 369
HinfI GANTC 1 cut(s) 79
HphI GGTGA 1 cut(s) 263
Hpy188I TCNGA 1 cut(s) 106
HpyCH4V TGCA 1 cut(s) 443
HpyF10VI GCNNNNNNNGC 4 cut(s) 296, 356, 428, 449
Hsp92II CATG 3 cut(s) 60, 340, 369
Kzo9I GATC 2 cut(s) 106, 226
LpnPI CCDG 6 cut(s) 105, 207, 234, 234, 263, 369
Lsp1109I GCAGC 2 cut(s) 334, 455
LweI GCATC 2 cut(s) 25, 250
MaeIII GTNAC 1 cut(s) 251
MalI GATC 2 cut(s) 108, 228
MboI GATC 2 cut(s) 106, 226
MboII GAAGA 2 cut(s) 341, 344
MflI RGATCY 2 cut(s) 106, 226
MhlI GDGCHC 1 cut(s) 221
MlsI TGGCCA 1 cut(s) 28
MluCI AATT 2 cut(s) 177, 458
MluNI TGGCCA 1 cut(s) 28
MnlI CCTC 5 cut(s) 120, 327, 349, 381, 405
Mox20I TGGCCA 1 cut(s) 28
MscI TGGCCA 1 cut(s) 28
MseI TTAA 3 cut(s) 165, 210, 374
MslI CAYNNNNRTG 1 cut(s) 149
Msp20I TGGCCA 1 cut(s) 28
MspCI CTTAAG 1 cut(s) 209
MspR9I CCNGG 1 cut(s) 222
MvaI CCWGG 1 cut(s) 222
MvnI CGCG 1 cut(s) 431
MwoI GCNNNNNNNGC 4 cut(s) 296, 356, 428, 449
NdeII GATC 2 cut(s) 106, 226
NlaIII CATG 3 cut(s) 60, 340, 369
NlaIV GGNNCC 2 cut(s) 124, 282
NmuCI GTSAC 1 cut(s) 251
NspI RCATGY 2 cut(s) 60, 369
PaeI GCATGC 1 cut(s) 369
PciI ACATGT 1 cut(s) 56
PfeI GAWTC 1 cut(s) 79
PkrI GCNGC 2 cut(s) 349, 445
PpuMI RGGWCCY 1 cut(s) 280
PscI ACATGT 1 cut(s) 56
Psp5II RGGWCCY 1 cut(s) 280
Psp6I CCWGG 1 cut(s) 220
PspGI CCWGG 1 cut(s) 220
PspN4I GGNNCC 2 cut(s) 124, 282
PspPI GGNCC 1 cut(s) 280
PspPPI RGGWCCY 1 cut(s) 280
PstI CTGCAG 1 cut(s) 445
PsuI RGATCY 2 cut(s) 106, 226
RseI CAYNNNNRTG 1 cut(s) 149
SaqAI TTAA 3 cut(s) 165, 210, 374
SatI GCNGC 2 cut(s) 348, 444
Sau3AI GATC 2 cut(s) 106, 226
Sau96I GGNCC 1 cut(s) 280
ScrFI CCNGG 1 cut(s) 222
SduI GDGCHC 1 cut(s) 221
SetI ASST 5 cut(s) 253, 282, 349, 392, 448
SfaNI GCATC 2 cut(s) 25, 250
SfcI CTRYAG 2 cut(s) 441, 447
SinI GGWCC 1 cut(s) 280
SmiMI CAYNNNNRTG 1 cut(s) 149
SmlI CTYRAG 1 cut(s) 209
SmoI CTYRAG 1 cut(s) 209
SphI GCATGC 1 cut(s) 369
Sse9I AATT 2 cut(s) 177, 458
SsiI CCGC 1 cut(s) 465
StyD4I CCNGG 1 cut(s) 220
StyI CCWWGG 1 cut(s) 294
TaqI TCGA 2 cut(s) 61, 112
TaqII GACCGA 1 cut(s) 173
TasI AATT 2 cut(s) 177, 458
TfiI GAWTC 1 cut(s) 79
Tru1I TTAA 3 cut(s) 165, 210, 374
Tru9I TTAA 3 cut(s) 165, 210, 374
TseFI GTSAC 1 cut(s) 251
TseI GCWGC 2 cut(s) 347, 443
Tsp45I GTSAC 1 cut(s) 251
Vha464I CTTAAG 1 cut(s) 209
VpaK11BI GGWCC 1 cut(s) 280
XceI RCATGY 2 cut(s) 60, 369
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.