Rw3G022400

Cell wall vacuolar inhibitor of fructosidase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0000921
Physical Location & Seq
Forward (+)
27410487 .. 27411011
525 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G022400.1

Sequence Viewer

Length: 525 bp
ATGAAGGATAGTTTAATCTCCATATTTCTAGTTCAACTTGTCTGTTCACTTCTGCTTCTTCCAATAGGCCAATGCAGCAGTGTTTTCTTTCCGATGGATGCGAAACTCATCGACCAAACGTGTAGAAAAACACCGGATTACAACCTTTGTACCTCAATTCTCAAATCTAGCCCCGGTAAAGATCTTAGAGGTTTAGCTATCGTAGCTGTTGACGCAGTTGGGTCTAAAGCAACTACAGCAATGAACAGAATTAATGAGTTGCTTAAACAAAGCCCAAAAGATACTGCCTTAAATCATTGTCATGATATGTACAATGTAATTTTAAATTCATATGTTCAAGGAGCCAGGGATACGCTTAAGCGGGGTTTCTCCTCTCATATTGCAACCTCAAGCATGAGTCTCACTCCCGATACTGCAAGGTCATGCGAGGAAGCTTTTAAAGGCAGGTCTCCTCTTACTAATTTGAACAAAGATGTCGAGAATATTGCGGCAGTGGCTGGAGCAGTGGCAGGGCAATTGCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

174

Amino Acids

18.72

Weight (kDa)

8.55

Isoelectric Point (pI)

45.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 35 - 163 1.8e-18 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 435
AciI CCGC 2 cut(s) 361, 488
AcsI RAATTY 1 cut(s) 325
AfaI GTAC 2 cut(s) 151, 311
AflII CTTAAG 1 cut(s) 356
AflIII ACRYGT 1 cut(s) 119
AgsI TTSAA 3 cut(s) 35, 338, 466
AjnI CCWGG 1 cut(s) 344
AluBI AGCT 3 cut(s) 197, 206, 434
AluI AGCT 3 cut(s) 197, 206, 434
Alw26I GTCTC 2 cut(s) 404, 453
AlwNI CAGNNNCTG 1 cut(s) 497
AoxI GGCC 1 cut(s) 67
ApeKI GCWGC 1 cut(s) 75
ApoI RAATTY 1 cut(s) 325
AseI ATTAAT 1 cut(s) 252
AsuC2I CCSGG 1 cut(s) 174
BaeI ACNNNNGTAYC 2 cut(s) 133, 166
BbvI GCAGC 1 cut(s) 87
BccI CCATC 1 cut(s) 88
BcgI CGANNNNNNTGC 2 cut(s) 467, 501
BciT130I CCWGG 1 cut(s) 346
BciVI GTATCC 1 cut(s) 343
BcnI CCSGG 1 cut(s) 174
BcoDI GTCTC 2 cut(s) 404, 453
BfaI CTAG 2 cut(s) 29, 168
BfmI CTRYAG 1 cut(s) 234
BfrI CTTAAG 1 cut(s) 356
BfuAI ACCTGC 1 cut(s) 435
BfuI GTATCC 1 cut(s) 343
BglII AGATCT 1 cut(s) 181
BisI GCNGC 2 cut(s) 76, 489
BlsI GCNGC 2 cut(s) 77, 490
Bme1390I CCNGG 2 cut(s) 174, 346
BmiI GGNNCC 1 cut(s) 343
BmrFI CCNGG 2 cut(s) 174, 346
BmsI GCATC 1 cut(s) 88
BplI GAGNNNNNCTC 2 cut(s) 388, 420
BpmI CTGGAG 1 cut(s) 519
BpuEI CTTGAG 1 cut(s) 373
BpuMI CCSGG 1 cut(s) 174
BsaI GGTCTC 1 cut(s) 453
BsaJI CCNNGG 2 cut(s) 172, 345
BsaWI WCCGGW 1 cut(s) 133
Bse3DI GCAATG 1 cut(s) 246
BseBI CCWGG 1 cut(s) 346
BseDI CCNNGG 2 cut(s) 172, 345
BseGI GGATG 1 cut(s) 103
BseMI GCAATG 1 cut(s) 246
BseRI GAGGAG 2 cut(s) 361, 441
BseXI GCAGC 1 cut(s) 87
BshFI GGCC 1 cut(s) 69
BsiSI CCGG 2 cut(s) 134, 174
BsmAI GTCTC 2 cut(s) 404, 453
BsnI GGCC 1 cut(s) 69
Bso31I GGTCTC 1 cut(s) 453
Bsp1407I TGTACA 1 cut(s) 309
Bsp143I GATC 1 cut(s) 181
BspACI CCGC 2 cut(s) 361, 488
BspANI GGCC 1 cut(s) 69
BspHI TCATGA 1 cut(s) 301
BspLI GGNNCC 1 cut(s) 343
BspMI ACCTGC 1 cut(s) 435
BspTI CTTAAG 1 cut(s) 356
BspTNI GGTCTC 1 cut(s) 453
BsrDI GCAATG 1 cut(s) 246
BsrGI TGTACA 1 cut(s) 309
BssECI CCNNGG 2 cut(s) 172, 345
BssMI GATC 1 cut(s) 181
Bst2UI CCWGG 1 cut(s) 346
BstAFI CTTAAG 1 cut(s) 356
BstAUI TGTACA 1 cut(s) 309
BstDEI CTNAG 1 cut(s) 185
BstF5I GGATG 1 cut(s) 103
BstKTI GATC 1 cut(s) 184
BstMAI GTCTC 2 cut(s) 404, 453
BstMBI GATC 1 cut(s) 181
BstMWI GCNNNNNNNGC 5 cut(s) 75, 203, 212, 236, 494
BstNI CCWGG 1 cut(s) 346
BstSCI CCNGG 2 cut(s) 172, 344
BstSFI CTRYAG 1 cut(s) 234
BstV1I GCAGC 1 cut(s) 87
BstX2I RGATCY 1 cut(s) 181
BstYI RGATCY 1 cut(s) 181
BsuI GTATCC 1 cut(s) 343
BsuRI GGCC 1 cut(s) 69
BtsCI GGATG 1 cut(s) 103
BtsI GCAGTG 3 cut(s) 85, 498, 510
BtsIMutI CAGTG 3 cut(s) 85, 498, 510
BveI ACCTGC 1 cut(s) 435
CaiI CAGNNNCTG 1 cut(s) 497
CciI TCATGA 1 cut(s) 301
CseI GACGC 1 cut(s) 221
Csp6I GTAC 2 cut(s) 150, 310
CviAII CATG 3 cut(s) 302, 394, 423
CviJI RGCY 8 cut(s) 69, 171, 197, 206, 273, 344, 434, 497
CviKI_1 RGCY 8 cut(s) 69, 171, 197, 206, 273, 344, 434, 497
CviQI GTAC 2 cut(s) 150, 310
DdeI CTNAG 1 cut(s) 185
DpnI GATC 1 cut(s) 183
DpnII GATC 1 cut(s) 181
DraI TTTAAA 2 cut(s) 324, 439
Eco31I GGTCTC 1 cut(s) 453
EcoRII CCWGG 1 cut(s) 344
FaeI CATG 3 cut(s) 305, 397, 426
FaiI YATR 8 cut(s) 23, 303, 308, 331, 333, 378, 395, 424
FatI CATG 3 cut(s) 301, 393, 422
FauI CCCGC 1 cut(s) 354
FauNDI CATATG 1 cut(s) 331
Fnu4HI GCNGC 2 cut(s) 76, 489
FokI GGATG 1 cut(s) 110
Fsp4HI GCNGC 2 cut(s) 76, 489
FspBI CTAG 2 cut(s) 29, 168
GluI GCNGC 2 cut(s) 76, 489
GsuI CTGGAG 1 cut(s) 519
HaeIII GGCC 1 cut(s) 69
HapII CCGG 2 cut(s) 134, 174
HgaI GACGC 1 cut(s) 221
Hin1II CATG 3 cut(s) 305, 397, 426
HincII GTYRAC 1 cut(s) 211
HindII GTYRAC 1 cut(s) 211
HindIII AAGCTT 1 cut(s) 432
HinfI GANTC 1 cut(s) 397
HpaII CCGG 2 cut(s) 134, 174
Hpy166II GTNNAC 2 cut(s) 47, 211
Hpy188I TCNGA 1 cut(s) 93
Hpy188III TCNNGA 3 cut(s) 302, 407, 478
Hpy8I GTNNAC 2 cut(s) 47, 211
HpyCH4IV ACGT 1 cut(s) 119
HpyCH4V TGCA 3 cut(s) 75, 383, 416
HpyF10VI GCNNNNNNNGC 5 cut(s) 75, 203, 212, 236, 494
HpyF3I CTNAG 1 cut(s) 185
HpySE526I ACGT 1 cut(s) 119
Hsp92II CATG 3 cut(s) 305, 397, 426
Kzo9I GATC 1 cut(s) 181
LmnI GCTCC 2 cut(s) 341, 500
LpnPI CCDG 7 cut(s) 147, 187, 331, 358, 430, 483, 495
Lsp1109I GCAGC 1 cut(s) 87
LweI GCATC 1 cut(s) 88
MaeI CTAG 2 cut(s) 29, 168
MaeII ACGT 1 cut(s) 119
MalI GATC 1 cut(s) 183
MboI GATC 1 cut(s) 181
MboII GAAGA 1 cut(s) 50
MfeI CAATTG 1 cut(s) 515
MflI RGATCY 1 cut(s) 181
MluCI AATT 6 cut(s) 156, 249, 318, 325, 460, 515
MlyI GAGTC 1 cut(s) 406
MnlI CCTC 6 cut(s) 163, 182, 382, 397, 421, 462
MseI TTAA 7 cut(s) 14, 252, 264, 290, 323, 357, 438
MslI CAYNNNNRTG 1 cut(s) 300
MspCI CTTAAG 1 cut(s) 356
MspI CCGG 2 cut(s) 134, 174
MspR9I CCNGG 2 cut(s) 174, 346
MunI CAATTG 1 cut(s) 515
MvaI CCWGG 1 cut(s) 346
MwoI GCNNNNNNNGC 5 cut(s) 75, 203, 212, 236, 494
NciI CCSGG 1 cut(s) 174
NdeI CATATG 1 cut(s) 331
NdeII GATC 1 cut(s) 181
NlaIII CATG 3 cut(s) 305, 397, 426
NlaIV GGNNCC 1 cut(s) 343
PagI TCATGA 1 cut(s) 301
PkrI GCNGC 2 cut(s) 77, 490
PleI GAGTC 1 cut(s) 405
PpsI GAGTC 1 cut(s) 405
PshBI ATTAAT 1 cut(s) 252
Psp6I CCWGG 1 cut(s) 344
PspGI CCWGG 1 cut(s) 344
PspN4I GGNNCC 1 cut(s) 343
PstNI CAGNNNCTG 1 cut(s) 497
PsuI RGATCY 1 cut(s) 181
RsaI GTAC 2 cut(s) 151, 311
RsaNI GTAC 2 cut(s) 150, 310
RseI CAYNNNNRTG 1 cut(s) 300
SaqAI TTAA 7 cut(s) 14, 252, 264, 290, 323, 357, 438
SatI GCNGC 2 cut(s) 76, 489
Sau3AI GATC 1 cut(s) 181
SchI GAGTC 1 cut(s) 406
ScrFI CCNGG 2 cut(s) 174, 346
SfaNI GCATC 1 cut(s) 88
SfcI CTRYAG 1 cut(s) 234
SmiMI CAYNNNNRTG 1 cut(s) 300
SmlI CTYRAG 2 cut(s) 356, 388
SmoI CTYRAG 2 cut(s) 356, 388
Sse9I AATT 6 cut(s) 156, 249, 318, 325, 460, 515
SsiI CCGC 2 cut(s) 361, 488
SspI AATATT 1 cut(s) 484
SspMI CTAG 2 cut(s) 29, 168
StyD4I CCNGG 2 cut(s) 172, 344
TaiI ACGT 1 cut(s) 122
TaqI TCGA 2 cut(s) 111, 477
TasI AATT 6 cut(s) 156, 249, 318, 325, 460, 515
TatI WGTACW 1 cut(s) 309
TauI GCSGC 1 cut(s) 491
Tru1I TTAA 7 cut(s) 14, 252, 264, 290, 323, 357, 438
Tru9I TTAA 7 cut(s) 14, 252, 264, 290, 323, 357, 438
TscAI CASTG 3 cut(s) 85, 498, 510
TseI GCWGC 1 cut(s) 75
TspDTI ATGAA 3 cut(s) 17, 257, 318
TspRI CASTG 3 cut(s) 85, 498, 510
Vha464I CTTAAG 1 cut(s) 356
VspI ATTAAT 1 cut(s) 252
XapI RAATTY 1 cut(s) 325
XspI CTAG 2 cut(s) 29, 168
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.