Rh2CG425000

Cell wall vacuolar inhibitor of fructosidase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0000921
Physical Location & Seq
Reverse (-)
57888715 .. 57889381
667 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG425000.1

Sequence Viewer

Length: 507 bp
ATGAAGAATTTGATGTGCTTAGCGGCAGTACTCATATTTTTCATTCAAACTGCAATTTTACCAACCGAAGCAAATATCATTGCCCAAACATGTCAAAAAACACCAAACCCTCCGGTGTGTCTCTCGTCTCTCAAATCCGACCCTCGGAGCGCCCAAGCAGACATATATGGCTTGGCTACCATAATGGTCGATGTGGTCAAGGCAAAGTCCACGACGACTCTGAACCAAATCAACCAATTGCTTAAGCAAAGCCCGAGAGACAAAGGCTTAATAGATTGTGCTGACAGTTACAACGCAGTTTTAAAAGGTGATGTCCCAGAAGCCTATGAGGCAATCAATGTAAGAAGACGTATGTTTGCATTGGATGCCATGAATGACGCTGTGAATGAGGCTAATGGATGCGAAAGCAACTTTGCAGGCAGCAGCTCTCATCCCCTGACTAAATTGAATAAAGATGTGGCAGATGTTGCTAGTGTGGCTGCAGCAGTTATTAATCAATTTGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

168

Amino Acids

17.97

Weight (kDa)

5.77

Isoelectric Point (pI)

33.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 26 - 162 4.4e-19 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 23
AcsI RAATTY 1 cut(s) 7
AfaI GTAC 1 cut(s) 30
AfiI CCNNNNNNNGG 1 cut(s) 144
AflII CTTAAG 1 cut(s) 242
AflIII ACRYGT 1 cut(s) 89
AgsI TTSAA 2 cut(s) 47, 448
AluBI AGCT 1 cut(s) 426
AluI AGCT 1 cut(s) 426
Alw26I GTCTC 3 cut(s) 125, 132, 252
Ama87I CYCGRG 1 cut(s) 253
ApeKI GCWGC 4 cut(s) 420, 423, 479, 482
ApoI RAATTY 1 cut(s) 7
AseI ATTAAT 1 cut(s) 492
AspLEI GCGC 1 cut(s) 152
AsuHPI GGTGA 1 cut(s) 320
AvaI CYCGRG 1 cut(s) 253
BbsI GAAGAC 1 cut(s) 352
BbvI GCAGC 4 cut(s) 432, 435, 466, 494
BcoDI GTCTC 3 cut(s) 125, 132, 252
BfaI CTAG 1 cut(s) 471
BfmI CTRYAG 1 cut(s) 480
BfoI RGCGCY 1 cut(s) 153
BfrI CTTAAG 1 cut(s) 242
BglI GCCNNNNNGGC 1 cut(s) 329
BisI GCNGC 5 cut(s) 24, 421, 424, 480, 483
BlpI GCTNAGC 1 cut(s) 19
BlsI GCNGC 5 cut(s) 25, 422, 425, 481, 484
BmcAI AGTACT 1 cut(s) 30
BmeT110I CYCGRG 1 cut(s) 253
BmsI GCATC 2 cut(s) 355, 389
BpiI GAAGAC 1 cut(s) 352
Bpu1102I GCTNAGC 1 cut(s) 19
BsaJI CCNNGG 1 cut(s) 143
BsaWI WCCGGW 1 cut(s) 112
Bsc4I CCNNNNNNNGG 1 cut(s) 144
Bse3DI GCAATG 1 cut(s) 78
BseDI CCNNGG 1 cut(s) 143
BseGI GGATG 3 cut(s) 370, 404, 430
BseLI CCNNNNNNNGG 1 cut(s) 144
BseMI GCAATG 1 cut(s) 78
BseXI GCAGC 4 cut(s) 432, 435, 466, 494
BsiHKCI CYCGRG 1 cut(s) 253
BsiSI CCGG 1 cut(s) 113
BslFI GGGAC 1 cut(s) 299
BslI CCNNNNNNNGG 1 cut(s) 144
BsmAI GTCTC 3 cut(s) 125, 132, 252
BsmBI CGTCTC 1 cut(s) 132
BsmFI GGGAC 1 cut(s) 299
BsoBI CYCGRG 1 cut(s) 253
Bsp1720I GCTNAGC 1 cut(s) 19
BspACI CCGC 1 cut(s) 23
BspMAI CTGCAG 1 cut(s) 484
BspTI CTTAAG 1 cut(s) 242
BsrDI GCAATG 1 cut(s) 78
BssECI CCNNGG 1 cut(s) 143
Bst4CI ACNGT 1 cut(s) 287
BstAFI CTTAAG 1 cut(s) 242
BstAPI GCANNNNNTGC 2 cut(s) 365, 467
BstC8I GCNNGC 1 cut(s) 418
BstDEI CTNAG 1 cut(s) 19
BstF5I GGATG 3 cut(s) 370, 404, 430
BstH2I RGCGCY 1 cut(s) 153
BstHHI GCGC 1 cut(s) 152
BstMAI GTCTC 3 cut(s) 125, 132, 252
BstMWI GCNNNNNNNGC 4 cut(s) 329, 365, 467, 476
BstNSI RCATGY 1 cut(s) 93
BstSFI CTRYAG 1 cut(s) 480
BstV1I GCAGC 4 cut(s) 432, 435, 466, 494
BstV2I GAAGAC 1 cut(s) 352
BtsCI GGATG 3 cut(s) 370, 404, 430
Cac8I GCNNGC 1 cut(s) 418
CfoI GCGC 1 cut(s) 152
CseI GACGC 1 cut(s) 386
Csp6I GTAC 1 cut(s) 29
CviAII CATG 2 cut(s) 90, 370
CviJI RGCY 8 cut(s) 171, 176, 252, 267, 323, 392, 426, 479
CviKI_1 RGCY 8 cut(s) 171, 176, 252, 267, 323, 392, 426, 479
CviQI GTAC 1 cut(s) 29
DdeI CTNAG 1 cut(s) 19
DraI TTTAAA 1 cut(s) 303
Eco88I CYCGRG 1 cut(s) 253
Esp3I CGTCTC 1 cut(s) 132
FaeI CATG 2 cut(s) 93, 373
FaiI YATR 9 cut(s) 35, 91, 164, 166, 168, 182, 327, 353, 371
FaqI GGGAC 1 cut(s) 299
FatI CATG 2 cut(s) 89, 369
Fnu4HI GCNGC 5 cut(s) 24, 421, 424, 480, 483
FokI GGATG 3 cut(s) 377, 411, 417
Fsp4HI GCNGC 5 cut(s) 24, 421, 424, 480, 483
FspBI CTAG 1 cut(s) 471
GlaI GCGC 1 cut(s) 151
GluI GCNGC 5 cut(s) 24, 421, 424, 480, 483
HaeII RGCGCY 1 cut(s) 153
HapII CCGG 1 cut(s) 113
HgaI GACGC 1 cut(s) 386
HhaI GCGC 1 cut(s) 152
Hin1II CATG 2 cut(s) 93, 373
Hin6I GCGC 1 cut(s) 150
HinP1I GCGC 1 cut(s) 150
HinfI GANTC 1 cut(s) 217
HpaII CCGG 1 cut(s) 113
HphI GGTGA 1 cut(s) 320
Hpy166II GTNNAC 1 cut(s) 210
Hpy188I TCNGA 3 cut(s) 139, 147, 222
Hpy8I GTNNAC 1 cut(s) 210
Hpy99I CGWCG 1 cut(s) 217
HpyCH4III ACNGT 1 cut(s) 287
HpyCH4IV ACGT 1 cut(s) 349
HpyCH4V TGCA 4 cut(s) 53, 359, 416, 482
HpyF10VI GCNNNNNNNGC 4 cut(s) 329, 365, 467, 476
HpyF3I CTNAG 1 cut(s) 19
HpySE526I ACGT 1 cut(s) 349
Hsp92II CATG 2 cut(s) 93, 373
HspAI GCGC 1 cut(s) 150
LmnI GCTCC 1 cut(s) 147
LpnPI CCDG 4 cut(s) 126, 330, 402, 449
Lsp1109I GCAGC 4 cut(s) 432, 435, 466, 494
LweI GCATC 2 cut(s) 355, 389
MaeI CTAG 1 cut(s) 471
MaeII ACGT 1 cut(s) 349
MaeIII GTNAC 1 cut(s) 287
MboII GAAGA 2 cut(s) 16, 357
MfeI CAATTG 1 cut(s) 236
MluCI AATT 5 cut(s) 7, 54, 236, 443, 497
MlyI GAGTC 1 cut(s) 211
MmeI TCCRAC 1 cut(s) 162
MnlI CCTC 4 cut(s) 120, 153, 322, 382
MseI TTAA 4 cut(s) 243, 269, 302, 492
MspCI CTTAAG 1 cut(s) 242
MspI CCGG 1 cut(s) 113
MunI CAATTG 1 cut(s) 236
MwoI GCNNNNNNNGC 4 cut(s) 329, 365, 467, 476
NlaIII CATG 2 cut(s) 93, 373
NspI RCATGY 1 cut(s) 93
PciI ACATGT 1 cut(s) 89
PkrI GCNGC 5 cut(s) 25, 422, 425, 481, 484
PleI GAGTC 1 cut(s) 211
PpsI GAGTC 1 cut(s) 211
PscI ACATGT 1 cut(s) 89
PshBI ATTAAT 1 cut(s) 492
PstI CTGCAG 1 cut(s) 484
RsaI GTAC 1 cut(s) 30
RsaNI GTAC 1 cut(s) 29
SaqAI TTAA 4 cut(s) 243, 269, 302, 492
SatI GCNGC 5 cut(s) 24, 421, 424, 480, 483
ScaI AGTACT 1 cut(s) 30
SchI GAGTC 1 cut(s) 211
SetI ASST 3 cut(s) 310, 352, 428
SfaNI GCATC 2 cut(s) 355, 389
SfcI CTRYAG 1 cut(s) 480
SmlI CTYRAG 1 cut(s) 242
SmoI CTYRAG 1 cut(s) 242
Sse9I AATT 5 cut(s) 7, 54, 236, 443, 497
SsiI CCGC 1 cut(s) 23
SspMI CTAG 1 cut(s) 471
TaaI ACNGT 1 cut(s) 287
TaiI ACGT 1 cut(s) 352
TaqI TCGA 1 cut(s) 189
TasI AATT 5 cut(s) 7, 54, 236, 443, 497
TatI WGTACW 1 cut(s) 28
TauI GCSGC 1 cut(s) 26
Tru1I TTAA 4 cut(s) 243, 269, 302, 492
Tru9I TTAA 4 cut(s) 243, 269, 302, 492
TseI GCWGC 4 cut(s) 420, 423, 479, 482
TspDTI ATGAA 3 cut(s) 17, 31, 386
Vha464I CTTAAG 1 cut(s) 242
VspI ATTAAT 1 cut(s) 492
XapI RAATTY 1 cut(s) 7
XceI RCATGY 1 cut(s) 93
XspI CTAG 1 cut(s) 471
ZrmI AGTACT 1 cut(s) 30
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.