FvH4_4g14140

Cell wall vacuolar inhibitor of fructosidase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0000921
Physical Location & Seq
Reverse (-)
17714546 .. 17714975
430 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g14140.t1

Sequence Viewer

Length: 345 bp
ATGGATGCTAAACTCATTGACGAAACATGTCAAAAAACACCAAACCCCAATCTCTGTGTCTCATCTCTCAAATCAGATCCTCGAAGTTCTGGTGCTGATGTCAAAGGCTTGGGCATAATAATGGTGGATGTGGTTAAGGCCAAAGCAATTATCGGTCAAAACAAAGTCCCAAATGCTTCCTTGGCATTTAGTAGAGATATCCCTCATATTGCTGAAGAAGACATGAGTGAAGCTGCTGACGAGGCAAGGGCATGCGAGTTAAGTTTTTCAGGGAGGTCTCCTCTAACAGATGTAAATAATGCTGTGGCAGATGTCGCGGCTATTACTGCAGCTATAGCAAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

115

Amino Acids

11.87

Weight (kDa)

4.7

Isoelectric Point (pI)

37.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 4 - 58 2.9e-09 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 317
AciI CCGC 1 cut(s) 317
AclWI GGATC 1 cut(s) 71
AcuI CTGAAG 1 cut(s) 234
AflIII ACRYGT 1 cut(s) 26
AluBI AGCT 2 cut(s) 233, 332
AluI AGCT 2 cut(s) 233, 332
Alw26I GTCTC 2 cut(s) 64, 282
AlwI GGATC 1 cut(s) 71
AoxI GGCC 1 cut(s) 138
ApeKI GCWGC 2 cut(s) 233, 329
BbsI GAAGAC 1 cut(s) 225
BbvI GCAGC 2 cut(s) 220, 341
BcoDI GTCTC 2 cut(s) 64, 282
BfmI CTRYAG 2 cut(s) 327, 333
BisI GCNGC 3 cut(s) 234, 318, 330
BlsI GCNGC 3 cut(s) 235, 319, 331
BpiI GAAGAC 1 cut(s) 225
BplI GAGNNNNNCTC 2 cut(s) 265, 297
BsaI GGTCTC 1 cut(s) 282
BsaJI CCNNGG 1 cut(s) 180
BseDI CCNNGG 1 cut(s) 180
BseGI GGATG 2 cut(s) 10, 133
BseRI GAGGAG 1 cut(s) 270
BseXI GCAGC 2 cut(s) 220, 341
Bsh1236I CGCG 1 cut(s) 317
BshFI GGCC 1 cut(s) 140
BslFI GGGAC 1 cut(s) 152
BsmAI GTCTC 2 cut(s) 64, 282
BsmFI GGGAC 1 cut(s) 152
BsnI GGCC 1 cut(s) 140
Bso31I GGTCTC 1 cut(s) 282
Bsp143I GATC 1 cut(s) 76
BspACI CCGC 1 cut(s) 317
BspANI GGCC 1 cut(s) 140
BspFNI CGCG 1 cut(s) 317
BspMAI CTGCAG 1 cut(s) 331
BspPI GGATC 1 cut(s) 71
BspTNI GGTCTC 1 cut(s) 282
BssECI CCNNGG 1 cut(s) 180
BssMI GATC 1 cut(s) 76
BssT1I CCWWGG 1 cut(s) 180
BstC8I GCNNGC 1 cut(s) 253
BstF5I GGATG 2 cut(s) 10, 133
BstFNI CGCG 1 cut(s) 317
BstKTI GATC 1 cut(s) 79
BstMAI GTCTC 2 cut(s) 64, 282
BstMBI GATC 1 cut(s) 76
BstMWI GCNNNNNNNGC 5 cut(s) 182, 242, 314, 326, 335
BstNSI RCATGY 2 cut(s) 30, 255
BstSFI CTRYAG 2 cut(s) 327, 333
BstUI CGCG 1 cut(s) 317
BstV1I GCAGC 2 cut(s) 220, 341
BstV2I GAAGAC 1 cut(s) 225
BstX2I RGATCY 1 cut(s) 76
BstYI RGATCY 1 cut(s) 76
BsuRI GGCC 1 cut(s) 140
BtsCI GGATG 2 cut(s) 10, 133
Cac8I GCNNGC 1 cut(s) 253
CviAII CATG 3 cut(s) 27, 223, 252
CviJI RGCY 5 cut(s) 108, 140, 233, 320, 332
CviKI_1 RGCY 5 cut(s) 108, 140, 233, 320, 332
DpnI GATC 1 cut(s) 78
DpnII GATC 1 cut(s) 76
Eco130I CCWWGG 1 cut(s) 180
Eco31I GGTCTC 1 cut(s) 282
Eco32I GATATC 1 cut(s) 199
Eco57I CTGAAG 1 cut(s) 234
EcoRV GATATC 1 cut(s) 199
EcoT14I CCWWGG 1 cut(s) 180
ErhI CCWWGG 1 cut(s) 180
FaeI CATG 3 cut(s) 30, 226, 255
FaiI YATR 6 cut(s) 28, 116, 207, 224, 253, 335
FaqI GGGAC 1 cut(s) 152
FatI CATG 3 cut(s) 26, 222, 251
Fnu4HI GCNGC 3 cut(s) 234, 318, 330
FokI GGATG 2 cut(s) 17, 140
Fsp4HI GCNGC 3 cut(s) 234, 318, 330
GluI GCNGC 3 cut(s) 234, 318, 330
HaeIII GGCC 1 cut(s) 140
Hin1II CATG 3 cut(s) 30, 226, 255
Hpy188I TCNGA 1 cut(s) 76
HpyCH4V TGCA 1 cut(s) 329
HpyF10VI GCNNNNNNNGC 5 cut(s) 182, 242, 314, 326, 335
Hsp92II CATG 3 cut(s) 30, 226, 255
Kzo9I GATC 1 cut(s) 76
LpnPI CCDG 2 cut(s) 75, 255
Lsp1109I GCAGC 2 cut(s) 220, 341
MalI GATC 1 cut(s) 78
MboI GATC 1 cut(s) 76
MboII GAAGA 2 cut(s) 227, 230
MflI RGATCY 1 cut(s) 76
MluCI AATT 1 cut(s) 147
MnlI CCTC 5 cut(s) 90, 213, 235, 267, 291
MseI TTAA 2 cut(s) 135, 260
MslI CAYNNNNRTG 1 cut(s) 119
MvnI CGCG 1 cut(s) 317
MwoI GCNNNNNNNGC 5 cut(s) 182, 242, 314, 326, 335
NdeII GATC 1 cut(s) 76
NlaIII CATG 3 cut(s) 30, 226, 255
NspI RCATGY 2 cut(s) 30, 255
PaeI GCATGC 1 cut(s) 255
PciI ACATGT 1 cut(s) 26
PkrI GCNGC 3 cut(s) 235, 319, 331
PscI ACATGT 1 cut(s) 26
PstI CTGCAG 1 cut(s) 331
PsuI RGATCY 1 cut(s) 76
RseI CAYNNNNRTG 1 cut(s) 119
SaqAI TTAA 2 cut(s) 135, 260
SatI GCNGC 3 cut(s) 234, 318, 330
Sau3AI GATC 1 cut(s) 76
SetI ASST 3 cut(s) 235, 278, 334
SfcI CTRYAG 2 cut(s) 327, 333
SmiMI CAYNNNNRTG 1 cut(s) 119
SphI GCATGC 1 cut(s) 255
Sse9I AATT 1 cut(s) 147
SsiI CCGC 1 cut(s) 317
StyI CCWWGG 1 cut(s) 180
TaqI TCGA 1 cut(s) 82
TaqII GACCGA 1 cut(s) 143
TasI AATT 1 cut(s) 147
TauI GCSGC 1 cut(s) 320
Tru1I TTAA 2 cut(s) 135, 260
Tru9I TTAA 2 cut(s) 135, 260
TseI GCWGC 2 cut(s) 233, 329
XceI RCATGY 2 cut(s) 30, 255
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.