FvH4_5g04330

Cell wall vacuolar inhibitor of fructosidase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0000921
Physical Location & Seq
Reverse (-)
2562904 .. 2563582
679 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g04330.t1

Sequence Viewer

Length: 591 bp
ATGAAGAGCAAATCAATATTTGTAGTAGCTCTCGTATTTCTCATCCAATCCCTGTTTCTTCCAATAAGCCATGGCGATGCCGATCTCATTAAACAAACATGCAGACATACACCGGATCAGCATCTCTGTGTCTCATCCCTCATAGCAGACCCTCGGAGCGCCAGCGCAGATGTCACAGGCTTGGCGCTCATTATGGTAGATGTTGTCAAGGCCAAGGCAATAGAATGTGCCAACAGAATCGACCACTTGCTCAAGCACAGGCCTGGAGACCAAGCCTTAAGAAATTGCAAGAGAATATACAGTGTAGTTTTAACTGCTGATGTGACCGTTGCAGTTGAGGCTTTAACCAAAGGTAACCTTCAGTTTGCAGAGCAAAGCATGGACGACATCGCCATGTTCGAGGCTGTTTTATGTGAAGATGGCTTTATAGGGGCTTCTCCCATGACAGATTTAAACAACGCTTCTCAGAAACTTGGAGCTGTGGCTGCAGCAATTGAGATCTGTAAGGCCCATCCGGATTGGATTCCGGCAGCAATTGAGGCTAACAAAATGATCTTCCTCAATGGGGTTGTTCTTGTGGACGGTTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

197

Amino Acids

21.1

Weight (kDa)

5.62

Isoelectric Point (pI)

32.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 28 - 165 1.1e-17 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 514
AclWI GGATC 1 cut(s) 123
AcuI CTGAAG 1 cut(s) 344
AfiI CCNNNNNNNGG 1 cut(s) 565
AflII CTTAAG 1 cut(s) 277
AjnI CCWGG 1 cut(s) 262
AjuI GAANNNNNNNTTGG 2 cut(s) 39, 71
AluBI AGCT 2 cut(s) 29, 479
AluI AGCT 2 cut(s) 29, 479
Alw26I GTCTC 2 cut(s) 136, 261
AlwI GGATC 1 cut(s) 123
Aor13HI TCCGGA 1 cut(s) 514
AoxI GGCC 3 cut(s) 210, 260, 507
ApeKI GCWGC 3 cut(s) 485, 488, 530
AspLEI GCGC 3 cut(s) 161, 167, 187
AspS9I GGNCC 1 cut(s) 508
BbvI GCAGC 3 cut(s) 472, 500, 542
BccI CCATC 2 cut(s) 413, 519
BciT130I CCWGG 1 cut(s) 264
BcoDI GTCTC 2 cut(s) 136, 261
BfmI CTRYAG 1 cut(s) 486
BfoI RGCGCY 2 cut(s) 162, 188
BfrI CTTAAG 1 cut(s) 277
BglII AGATCT 1 cut(s) 498
BisI GCNGC 3 cut(s) 486, 489, 531
BlsI GCNGC 3 cut(s) 487, 490, 532
Bme1390I CCNGG 1 cut(s) 264
BmgT120I GGNCC 1 cut(s) 508
BmrFI CCNGG 1 cut(s) 264
BmsI GCATC 2 cut(s) 67, 130
BpmI CTGGAG 1 cut(s) 285
BpuEI CTTGAG 1 cut(s) 236
BsaBI GATNNNNATC 2 cut(s) 81, 120
BsaI GGTCTC 1 cut(s) 261
BsaJI CCNNGG 3 cut(s) 70, 152, 213
BsaWI WCCGGW 2 cut(s) 112, 514
Bsc4I CCNNNNNNNGG 1 cut(s) 565
Bse8I GATNNNNATC 2 cut(s) 81, 120
BseAI TCCGGA 1 cut(s) 514
BseBI CCWGG 1 cut(s) 264
BseDI CCNNGG 3 cut(s) 70, 152, 213
BseGI GGATG 3 cut(s) 42, 134, 511
BseJI GATNNNNATC 2 cut(s) 81, 120
BseLI CCNNNNNNNGG 1 cut(s) 565
BseMII CTCAG 1 cut(s) 479
BseXI GCAGC 3 cut(s) 472, 500, 542
BshFI GGCC 3 cut(s) 212, 262, 509
BsiSI CCGG 3 cut(s) 113, 515, 527
BslI CCNNNNNNNGG 1 cut(s) 565
BsmAI GTCTC 2 cut(s) 136, 261
BsnI GGCC 3 cut(s) 212, 262, 509
Bso31I GGTCTC 1 cut(s) 261
Bsp13I TCCGGA 1 cut(s) 514
Bsp143I GATC 4 cut(s) 82, 115, 498, 552
Bsp19I CCATGG 1 cut(s) 70
BspANI GGCC 3 cut(s) 212, 262, 509
BspCNI CTCAG 1 cut(s) 478
BspEI TCCGGA 1 cut(s) 514
BspMAI CTGCAG 1 cut(s) 490
BspPI GGATC 1 cut(s) 123
BspTI CTTAAG 1 cut(s) 277
BspTNI GGTCTC 1 cut(s) 261
BssECI CCNNGG 3 cut(s) 70, 152, 213
BssMI GATC 4 cut(s) 82, 115, 498, 552
BssT1I CCWWGG 2 cut(s) 70, 213
Bst2UI CCWGG 1 cut(s) 264
Bst4CI ACNGT 3 cut(s) 302, 328, 584
BstAFI CTTAAG 1 cut(s) 277
BstC8I GCNNGC 1 cut(s) 163
BstDEI CTNAG 1 cut(s) 465
BstDSI CCRYGG 1 cut(s) 70
BstEII GGTNACC 1 cut(s) 353
BstF5I GGATG 3 cut(s) 42, 134, 511
BstH2I RGCGCY 2 cut(s) 162, 188
BstHHI GCGC 3 cut(s) 161, 167, 187
BstKTI GATC 4 cut(s) 85, 118, 501, 555
BstMAI GTCTC 2 cut(s) 136, 261
BstMBI GATC 4 cut(s) 82, 115, 498, 552
BstMWI GCNNNNNNNGC 3 cut(s) 338, 485, 539
BstNI CCWGG 1 cut(s) 264
BstNSI RCATGY 1 cut(s) 102
BstPI GGTNACC 1 cut(s) 353
BstSCI CCNGG 1 cut(s) 262
BstSFI CTRYAG 1 cut(s) 486
BstV1I GCAGC 3 cut(s) 472, 500, 542
BstX2I RGATCY 1 cut(s) 498
BstYI RGATCY 1 cut(s) 498
BsuRI GGCC 3 cut(s) 212, 262, 509
BtgI CCRYGG 1 cut(s) 70
BtgZI GCGATG 2 cut(s) 90, 373
BtsCI GGATG 3 cut(s) 42, 134, 511
BtsIMutI CAGTG 1 cut(s) 307
Cac8I GCNNGC 1 cut(s) 163
CfoI GCGC 3 cut(s) 161, 167, 187
Cfr13I GGNCC 1 cut(s) 508
CviAII CATG 5 cut(s) 71, 99, 379, 394, 442
DdeI CTNAG 1 cut(s) 465
DpnI GATC 4 cut(s) 84, 117, 500, 554
DpnII GATC 4 cut(s) 82, 115, 498, 552
DraI TTTAAA 1 cut(s) 453
Eco130I CCWWGG 2 cut(s) 70, 213
Eco147I AGGCCT 1 cut(s) 262
Eco31I GGTCTC 1 cut(s) 261
Eco57I CTGAAG 1 cut(s) 344
Eco91I GGTNACC 1 cut(s) 353
EcoO65I GGTNACC 1 cut(s) 353
EcoRII CCWGG 1 cut(s) 262
EcoT14I CCWWGG 2 cut(s) 70, 213
ErhI CCWWGG 2 cut(s) 70, 213
FaeI CATG 5 cut(s) 74, 102, 382, 397, 445
FalI AAGNNNNNCTT 2 cut(s) 342, 374
FatI CATG 5 cut(s) 70, 98, 378, 393, 441
Fnu4HI GCNGC 3 cut(s) 486, 489, 531
FokI GGATG 3 cut(s) 29, 121, 498
Fsp4HI GCNGC 3 cut(s) 486, 489, 531
GlaI GCGC 3 cut(s) 160, 166, 186
GluI GCNGC 3 cut(s) 486, 489, 531
GsuI CTGGAG 1 cut(s) 285
HaeII RGCGCY 2 cut(s) 162, 188
HaeIII GGCC 3 cut(s) 212, 262, 509
HapII CCGG 3 cut(s) 113, 515, 527
HhaI GCGC 3 cut(s) 161, 167, 187
Hin1II CATG 5 cut(s) 74, 102, 382, 397, 445
Hin6I GCGC 3 cut(s) 159, 165, 185
HinP1I GCGC 3 cut(s) 159, 165, 185
HinfI GANTC 2 cut(s) 237, 523
HpaII CCGG 3 cut(s) 113, 515, 527
Hpy166II GTNNAC 1 cut(s) 580
Hpy188I TCNGA 2 cut(s) 156, 468
Hpy188III TCNNGA 1 cut(s) 515
Hpy8I GTNNAC 1 cut(s) 580
HpyAV CCTTC 1 cut(s) 368
HpyCH4III ACNGT 3 cut(s) 302, 328, 584
HpyCH4V TGCA 5 cut(s) 102, 288, 332, 368, 488
HpyF10VI GCNNNNNNNGC 3 cut(s) 338, 485, 539
HpyF3I CTNAG 1 cut(s) 465
Hsp92II CATG 5 cut(s) 74, 102, 382, 397, 445
HspAI GCGC 3 cut(s) 159, 165, 185
Kpn2I TCCGGA 1 cut(s) 514
Kzo9I GATC 4 cut(s) 82, 115, 498, 552
LmnI GCTCC 2 cut(s) 156, 476
LpnPI CCDG 9 cut(s) 65, 126, 162, 175, 244, 249, 276, 528, 540
Lsp1109I GCAGC 3 cut(s) 472, 500, 542
LweI GCATC 2 cut(s) 67, 130
MaeIII GTNAC 3 cut(s) 172, 322, 353
MalI GATC 4 cut(s) 84, 117, 500, 554
MboI GATC 4 cut(s) 82, 115, 498, 552
MboII GAAGA 4 cut(s) 16, 50, 428, 547
MfeI CAATTG 2 cut(s) 492, 534
MflI RGATCY 1 cut(s) 498
MluCI AATT 3 cut(s) 283, 492, 534
MnlI CCTC 6 cut(s) 149, 162, 331, 394, 532, 569
MroI TCCGGA 1 cut(s) 514
MseI TTAA 5 cut(s) 90, 278, 311, 344, 452
MslI CAYNNNNRTG 3 cut(s) 75, 126, 392
MspCI CTTAAG 1 cut(s) 277
MspI CCGG 3 cut(s) 113, 515, 527
MspR9I CCNGG 1 cut(s) 264
MunI CAATTG 2 cut(s) 492, 534
MvaI CCWGG 1 cut(s) 264
MwoI GCNNNNNNNGC 3 cut(s) 338, 485, 539
NcoI CCATGG 1 cut(s) 70
NdeII GATC 4 cut(s) 82, 115, 498, 552
NlaIII CATG 5 cut(s) 74, 102, 382, 397, 445
NmuCI GTSAC 2 cut(s) 172, 322
NspI RCATGY 1 cut(s) 102
PceI AGGCCT 1 cut(s) 262
PcsI WCGNNNNNNNCGW 1 cut(s) 396
PfeI GAWTC 2 cut(s) 237, 523
PkrI GCNGC 3 cut(s) 487, 490, 532
Psp6I CCWGG 1 cut(s) 262
PspEI GGTNACC 1 cut(s) 353
PspGI CCWGG 1 cut(s) 262
PspPI GGNCC 1 cut(s) 508
PstI CTGCAG 1 cut(s) 490
PsuI RGATCY 1 cut(s) 498
RseI CAYNNNNRTG 3 cut(s) 75, 126, 392
SaqAI TTAA 5 cut(s) 90, 278, 311, 344, 452
SatI GCNGC 3 cut(s) 486, 489, 531
Sau3AI GATC 4 cut(s) 82, 115, 498, 552
Sau96I GGNCC 1 cut(s) 508
ScrFI CCNGG 1 cut(s) 264
SetI ASST 4 cut(s) 31, 355, 360, 481
SfaNI GCATC 2 cut(s) 67, 130
SfcI CTRYAG 1 cut(s) 486
SmiMI CAYNNNNRTG 3 cut(s) 75, 126, 392
SmlI CTYRAG 2 cut(s) 251, 277
SmoI CTYRAG 2 cut(s) 251, 277
Sse9I AATT 3 cut(s) 283, 492, 534
SseBI AGGCCT 1 cut(s) 262
SspI AATATT 1 cut(s) 18
StuI AGGCCT 1 cut(s) 262
StyD4I CCNGG 1 cut(s) 262
StyI CCWWGG 2 cut(s) 70, 213
TaaI ACNGT 3 cut(s) 302, 328, 584
TaqI TCGA 2 cut(s) 240, 399
TasI AATT 3 cut(s) 283, 492, 534
TfiI GAWTC 2 cut(s) 237, 523
Tru1I TTAA 5 cut(s) 90, 278, 311, 344, 452
Tru9I TTAA 5 cut(s) 90, 278, 311, 344, 452
TscAI CASTG 1 cut(s) 307
TseFI GTSAC 2 cut(s) 172, 322
TseI GCWGC 3 cut(s) 485, 488, 530
Tsp45I GTSAC 2 cut(s) 172, 322
TspDTI ATGAA 1 cut(s) 17
TspRI CASTG 1 cut(s) 307
Vha464I CTTAAG 1 cut(s) 277
XceI RCATGY 1 cut(s) 102
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.