RchiOBHm_Chr7g0200071

Cell wall vacuolar inhibitor of fructosidase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0000921
Physical Location & Seq
Reverse (-)
17976611 .. 17977255
645 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ17906

Sequence Viewer

Length: 645 bp
ATGTTTTCCTTAAAAACATATGGTCTTGAATCTCTTAGATTAATTTCTCTCCCCTGTTTATATGCAGAGAGATTCATCCCACAAATAGTTCTCCATATCATCAAGCATCCACCAAATTATCAATCCTTCGTCCTAAAAATGAAGAATTTGATGTGCTTAGCGGCAGTGCTCATATTTTTCATTCAAACTGCAATTTTACGAACCGAAGCAAATATCATTGCCCAAACATGTCAAAAAACACCAAACCCTCCGGTGTGTCTCTCGTCTCTCAAATCCGACCCTCGGAGCGCCCAAGCAGACATATATGGCTTGGCTATCATAATGGTCGATGTGGTCAAGGCAAAGTCCACGACGACTCTGAACCAAATCAACCAATTGCTTAAGCAAAGCCCGAGAGACAAAGGCTTAATAGATTGTGCTGACAGGTACAACGCAGTTTTAAAAGGTGATGTACCAGAAGCCTATGAGGCAATCAATGTAAGAAGACGTATGTTTGCATTGGATGGCATGAATGACGCTGTGAATGAGGCTAATGGATGCGAAAGAAACTTTGCAGGCAGAAGCTCTCATCCTCTGACTAAATTGAATAAAGATGTGGCAGATGTTGCTAGTGTGGCTGCAGCAGTTATTAATCAATTTGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

214

Amino Acids

23.64

Weight (kDa)

9.04

Isoelectric Point (pI)

37.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 72 - 208 2e-18 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 161
AcsI RAATTY 1 cut(s) 145
AfaI GTAC 2 cut(s) 428, 453
AfiI CCNNNNNNNGG 1 cut(s) 282
AflII CTTAAG 1 cut(s) 380
AflIII ACRYGT 1 cut(s) 227
AgsI TTSAA 3 cut(s) 29, 185, 586
AluBI AGCT 1 cut(s) 564
AluI AGCT 1 cut(s) 564
Alw21I GWGCWC 1 cut(s) 171
Alw26I GTCTC 3 cut(s) 263, 270, 390
Ama87I CYCGRG 1 cut(s) 391
ApeKI GCWGC 2 cut(s) 617, 620
ApoI RAATTY 1 cut(s) 145
AseI ATTAAT 2 cut(s) 41, 630
AspLEI GCGC 1 cut(s) 290
AsuHPI GGTGA 1 cut(s) 458
AvaI CYCGRG 1 cut(s) 391
BbsI GAAGAC 1 cut(s) 490
Bbv12I GWGCWC 1 cut(s) 171
BbvI GCAGC 2 cut(s) 604, 632
BccI CCATC 1 cut(s) 497
BcoDI GTCTC 3 cut(s) 263, 270, 390
BfaI CTAG 1 cut(s) 609
BfmI CTRYAG 1 cut(s) 618
BfoI RGCGCY 1 cut(s) 291
BfrI CTTAAG 1 cut(s) 380
BglI GCCNNNNNGGC 1 cut(s) 467
BisI GCNGC 3 cut(s) 162, 618, 621
BlpI GCTNAGC 1 cut(s) 157
BlsI GCNGC 3 cut(s) 163, 619, 622
BmeT110I CYCGRG 1 cut(s) 391
BmsI GCATC 2 cut(s) 115, 527
BpiI GAAGAC 1 cut(s) 490
Bpu1102I GCTNAGC 1 cut(s) 157
BsaJI CCNNGG 1 cut(s) 281
BsaWI WCCGGW 1 cut(s) 250
Bsc4I CCNNNNNNNGG 1 cut(s) 282
Bse3DI GCAATG 1 cut(s) 216
BseDI CCNNGG 1 cut(s) 281
BseGI GGATG 5 cut(s) 75, 106, 508, 542, 568
BseLI CCNNNNNNNGG 1 cut(s) 282
BseMI GCAATG 1 cut(s) 216
BseXI GCAGC 2 cut(s) 604, 632
BsiHKAI GWGCWC 1 cut(s) 171
BsiHKCI CYCGRG 1 cut(s) 391
BsiSI CCGG 1 cut(s) 251
BslI CCNNNNNNNGG 1 cut(s) 282
BsmAI GTCTC 3 cut(s) 263, 270, 390
BsmBI CGTCTC 1 cut(s) 270
BsoBI CYCGRG 1 cut(s) 391
Bsp1286I GDGCHC 1 cut(s) 171
Bsp1720I GCTNAGC 1 cut(s) 157
BspACI CCGC 1 cut(s) 161
BspMAI CTGCAG 1 cut(s) 622
BspTI CTTAAG 1 cut(s) 380
BsrDI GCAATG 1 cut(s) 216
BssECI CCNNGG 1 cut(s) 281
BstAFI CTTAAG 1 cut(s) 380
BstAPI GCANNNNNTGC 1 cut(s) 605
BstC8I GCNNGC 1 cut(s) 556
BstDEI CTNAG 2 cut(s) 35, 157
BstF5I GGATG 5 cut(s) 75, 106, 508, 542, 568
BstH2I RGCGCY 1 cut(s) 291
BstHHI GCGC 1 cut(s) 290
BstMAI GTCTC 3 cut(s) 263, 270, 390
BstMWI GCNNNNNNNGC 3 cut(s) 467, 605, 614
BstNSI RCATGY 1 cut(s) 231
BstSFI CTRYAG 1 cut(s) 618
BstV1I GCAGC 2 cut(s) 604, 632
BstV2I GAAGAC 1 cut(s) 490
BtsCI GGATG 5 cut(s) 75, 106, 508, 542, 568
BtsI GCAGTG 1 cut(s) 171
BtsIMutI CAGTG 1 cut(s) 171
Cac8I GCNNGC 1 cut(s) 556
CfoI GCGC 1 cut(s) 290
CseI GACGC 1 cut(s) 524
Csp6I GTAC 2 cut(s) 427, 452
CviAII CATG 2 cut(s) 228, 508
CviJI RGCY 8 cut(s) 309, 314, 390, 405, 461, 530, 564, 617
CviKI_1 RGCY 8 cut(s) 309, 314, 390, 405, 461, 530, 564, 617
CviQI GTAC 2 cut(s) 427, 452
DdeI CTNAG 2 cut(s) 35, 157
DraI TTTAAA 1 cut(s) 441
Eco88I CYCGRG 1 cut(s) 391
Esp3I CGTCTC 1 cut(s) 270
FaeI CATG 2 cut(s) 231, 511
FatI CATG 2 cut(s) 227, 507
FauNDI CATATG 1 cut(s) 19
Fnu4HI GCNGC 3 cut(s) 162, 618, 621
FokI GGATG 5 cut(s) 62, 93, 515, 549, 555
Fsp4HI GCNGC 3 cut(s) 162, 618, 621
FspBI CTAG 1 cut(s) 609
GlaI GCGC 1 cut(s) 289
GluI GCNGC 3 cut(s) 162, 618, 621
HaeII RGCGCY 1 cut(s) 291
HapII CCGG 1 cut(s) 251
HgaI GACGC 1 cut(s) 524
HhaI GCGC 1 cut(s) 290
Hin1II CATG 2 cut(s) 231, 511
Hin6I GCGC 1 cut(s) 288
HinP1I GCGC 1 cut(s) 288
HinfI GANTC 3 cut(s) 29, 72, 355
HpaII CCGG 1 cut(s) 251
HphI GGTGA 1 cut(s) 458
Hpy166II GTNNAC 1 cut(s) 348
Hpy188I TCNGA 4 cut(s) 277, 285, 360, 576
Hpy188III TCNNGA 1 cut(s) 26
Hpy8I GTNNAC 1 cut(s) 348
Hpy99I CGWCG 1 cut(s) 355
HpyAV CCTTC 1 cut(s) 136
HpyCH4IV ACGT 1 cut(s) 487
HpyCH4V TGCA 5 cut(s) 65, 191, 497, 554, 620
HpyF10VI GCNNNNNNNGC 3 cut(s) 467, 605, 614
HpyF3I CTNAG 2 cut(s) 35, 157
HpySE526I ACGT 1 cut(s) 487
Hsp92II CATG 2 cut(s) 231, 511
HspAI GCGC 1 cut(s) 288
LmnI GCTCC 1 cut(s) 285
LpnPI CCDG 5 cut(s) 67, 264, 409, 468, 540
Lsp1109I GCAGC 2 cut(s) 604, 632
LweI GCATC 2 cut(s) 115, 527
MaeI CTAG 1 cut(s) 609
MaeII ACGT 1 cut(s) 487
MboII GAAGA 2 cut(s) 154, 495
MfeI CAATTG 1 cut(s) 374
MhlI GDGCHC 1 cut(s) 171
MluCI AATT 7 cut(s) 42, 115, 145, 192, 374, 581, 635
MlyI GAGTC 1 cut(s) 349
MmeI TCCRAC 1 cut(s) 300
MnlI CCTC 5 cut(s) 258, 291, 460, 520, 582
MseI TTAA 6 cut(s) 11, 41, 381, 407, 440, 630
MspCI CTTAAG 1 cut(s) 380
MspI CCGG 1 cut(s) 251
MunI CAATTG 1 cut(s) 374
MwoI GCNNNNNNNGC 3 cut(s) 467, 605, 614
NdeI CATATG 1 cut(s) 19
NlaIII CATG 2 cut(s) 231, 511
NspI RCATGY 1 cut(s) 231
PciI ACATGT 1 cut(s) 227
PfeI GAWTC 2 cut(s) 29, 72
PkrI GCNGC 3 cut(s) 163, 619, 622
PleI GAGTC 1 cut(s) 349
PpsI GAGTC 1 cut(s) 349
PscI ACATGT 1 cut(s) 227
PshBI ATTAAT 2 cut(s) 41, 630
PstI CTGCAG 1 cut(s) 622
RsaI GTAC 2 cut(s) 428, 453
RsaNI GTAC 2 cut(s) 427, 452
SaqAI TTAA 6 cut(s) 11, 41, 381, 407, 440, 630
SatI GCNGC 3 cut(s) 162, 618, 621
SchI GAGTC 1 cut(s) 349
SduI GDGCHC 1 cut(s) 171
SetI ASST 4 cut(s) 428, 448, 490, 566
SfaNI GCATC 2 cut(s) 115, 527
SfcI CTRYAG 1 cut(s) 618
SmlI CTYRAG 1 cut(s) 380
SmoI CTYRAG 1 cut(s) 380
Sse9I AATT 7 cut(s) 42, 115, 145, 192, 374, 581, 635
SsiI CCGC 1 cut(s) 161
SspMI CTAG 1 cut(s) 609
TaiI ACGT 1 cut(s) 490
TaqI TCGA 1 cut(s) 327
TasI AATT 7 cut(s) 42, 115, 145, 192, 374, 581, 635
TauI GCSGC 1 cut(s) 164
TfiI GAWTC 2 cut(s) 29, 72
Tru1I TTAA 6 cut(s) 11, 41, 381, 407, 440, 630
Tru9I TTAA 6 cut(s) 11, 41, 381, 407, 440, 630
TscAI CASTG 1 cut(s) 171
TseI GCWGC 2 cut(s) 617, 620
TspDTI ATGAA 4 cut(s) 64, 155, 169, 524
TspRI CASTG 1 cut(s) 171
Vha464I CTTAAG 1 cut(s) 380
VspI ATTAAT 2 cut(s) 41, 630
XapI RAATTY 1 cut(s) 145
XceI RCATGY 1 cut(s) 231
XspI CTAG 1 cut(s) 609
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.