Rh2DG458200

Cell wall vacuolar inhibitor of fructosidase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0000921
Physical Location & Seq
Reverse (-)
66264813 .. 66298473
33661 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG458200.1

Sequence Viewer

Length: 711 bp
ATGAGAAATGGGACTCACCAATCACAATGCTTTCTCTGCTCAGCTTCAATTGAACAATTCTTCACTGTAGACGATGCTACTCAATTTGGGGATTTAGGGTTTTTGCTCCTCCGCTCACCAAGCGGGAACCGAAAGAGATTCATCCCACAAATAGTTCTCCATATCATCAAGCATCCACCAAATTATCAGTCCTTCGTCCTAAAAATGAAGAATTTGATGTGCTTAGCGGCAGTGCTCATATTTTTCATTCAAACTGCAATTTTACGAACCGAAGCAAATATCATTGCCCAAACATGTCAAAAAACACCAAACCCTCCGGTGTGTCTCTCGTCTCTCAAATCCGACCCTCGGAGCGCCCAAGCAGACATATATGGCTTGGCTATCATAATGGTCGATGTGGTCAAGGCAAAGTCCACGACGACTCTGAACCAAATCAACCAATTGCTTAAGCAAAGCCCGAGAGACAAAGGCTTAATAGATTGTGCTGACAGGTACAACGCAGTTTTAAAAGGTGATGTCCCAGAAGCCTATGAGGCAATCAATGTAAGAAGACGTATGTTTGCATTGGATGGCATGAATGACGCTGTGAATGAGGCTAATGGATGCGAAAGAAACTTTGCAGGCAGAAGCTCTCATCCTCTGACTAAATTGAATAAAGATGTGGCAGATGTTGCTAGTGTGGCTGCAGCAGTTATTAATCAATTTGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

236

Amino Acids

26.02

Weight (kDa)

8.99

Isoelectric Point (pI)

40.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 94 - 230 2.6e-18 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 114
AccI GTMKAC 1 cut(s) 69
AciI CCGC 3 cut(s) 112, 123, 227
AcsI RAATTY 1 cut(s) 211
AfaI GTAC 1 cut(s) 494
AfiI CCNNNNNNNGG 1 cut(s) 348
AflII CTTAAG 1 cut(s) 446
AflIII ACRYGT 1 cut(s) 293
AgsI TTSAA 4 cut(s) 48, 53, 251, 652
AluBI AGCT 2 cut(s) 44, 630
AluI AGCT 2 cut(s) 44, 630
Alw21I GWGCWC 1 cut(s) 237
Alw26I GTCTC 3 cut(s) 329, 336, 456
Ama87I CYCGRG 1 cut(s) 457
ApeKI GCWGC 2 cut(s) 683, 686
ApoI RAATTY 1 cut(s) 211
ArsI GACNNNNNNTTYG 2 cut(s) 173, 205
AseI ATTAAT 1 cut(s) 696
AspLEI GCGC 1 cut(s) 356
AsuHPI GGTGA 3 cut(s) 8, 108, 524
AvaI CYCGRG 1 cut(s) 457
BbsI GAAGAC 1 cut(s) 556
Bbv12I GWGCWC 1 cut(s) 237
BbvI GCAGC 2 cut(s) 670, 698
BccI CCATC 1 cut(s) 563
BcoDI GTCTC 3 cut(s) 329, 336, 456
BfaI CTAG 1 cut(s) 675
BfmI CTRYAG 2 cut(s) 66, 684
BfoI RGCGCY 1 cut(s) 357
BfrI CTTAAG 1 cut(s) 446
BglI GCCNNNNNGGC 1 cut(s) 533
BisI GCNGC 3 cut(s) 228, 684, 687
BlpI GCTNAGC 2 cut(s) 40, 223
BlsI GCNGC 3 cut(s) 229, 685, 688
BmeT110I CYCGRG 1 cut(s) 457
BmiI GGNNCC 1 cut(s) 128
BmsI GCATC 3 cut(s) 64, 181, 593
BpiI GAAGAC 1 cut(s) 556
Bpu1102I GCTNAGC 2 cut(s) 40, 223
BsaJI CCNNGG 1 cut(s) 347
BsaWI WCCGGW 1 cut(s) 316
Bsc4I CCNNNNNNNGG 1 cut(s) 348
Bse3DI GCAATG 1 cut(s) 282
BseDI CCNNGG 1 cut(s) 347
BseGI GGATG 5 cut(s) 141, 172, 574, 608, 634
BseLI CCNNNNNNNGG 1 cut(s) 348
BseMI GCAATG 1 cut(s) 282
BseMII CTCAG 1 cut(s) 54
BseRI GAGGAG 1 cut(s) 98
BseXI GCAGC 2 cut(s) 670, 698
BsiHKAI GWGCWC 1 cut(s) 237
BsiHKCI CYCGRG 1 cut(s) 457
BsiSI CCGG 1 cut(s) 317
BslFI GGGAC 2 cut(s) 25, 503
BslI CCNNNNNNNGG 1 cut(s) 348
BsmAI GTCTC 3 cut(s) 329, 336, 456
BsmBI CGTCTC 1 cut(s) 336
BsmFI GGGAC 2 cut(s) 25, 503
BsoBI CYCGRG 1 cut(s) 457
Bsp1286I GDGCHC 1 cut(s) 237
Bsp1720I GCTNAGC 2 cut(s) 40, 223
BspACI CCGC 3 cut(s) 112, 123, 227
BspCNI CTCAG 1 cut(s) 53
BspLI GGNNCC 1 cut(s) 128
BspMAI CTGCAG 1 cut(s) 688
BspTI CTTAAG 1 cut(s) 446
BsrBI CCGCTC 1 cut(s) 114
BsrDI GCAATG 1 cut(s) 282
BssECI CCNNGG 1 cut(s) 347
Bst4CI ACNGT 1 cut(s) 67
BstAFI CTTAAG 1 cut(s) 446
BstAPI GCANNNNNTGC 1 cut(s) 671
BstC8I GCNNGC 1 cut(s) 622
BstDEI CTNAG 2 cut(s) 40, 223
BstF5I GGATG 5 cut(s) 141, 172, 574, 608, 634
BstH2I RGCGCY 1 cut(s) 357
BstHHI GCGC 1 cut(s) 356
BstMAI GTCTC 3 cut(s) 329, 336, 456
BstMWI GCNNNNNNNGC 5 cut(s) 36, 120, 533, 671, 680
BstNSI RCATGY 1 cut(s) 297
BstSFI CTRYAG 2 cut(s) 66, 684
BstV1I GCAGC 2 cut(s) 670, 698
BstV2I GAAGAC 1 cut(s) 556
BtsCI GGATG 5 cut(s) 141, 172, 574, 608, 634
BtsI GCAGTG 1 cut(s) 237
BtsIMutI CAGTG 2 cut(s) 63, 237
Cac8I GCNNGC 1 cut(s) 622
CfoI GCGC 1 cut(s) 356
CseI GACGC 1 cut(s) 590
Csp6I GTAC 1 cut(s) 493
CviAII CATG 2 cut(s) 294, 574
CviJI RGCY 9 cut(s) 44, 375, 380, 456, 471, 527, 596, 630, 683
CviKI_1 RGCY 9 cut(s) 44, 375, 380, 456, 471, 527, 596, 630, 683
CviQI GTAC 1 cut(s) 493
DdeI CTNAG 2 cut(s) 40, 223
DraI TTTAAA 1 cut(s) 507
Eco88I CYCGRG 1 cut(s) 457
Esp3I CGTCTC 1 cut(s) 336
FaeI CATG 2 cut(s) 297, 577
FaqI GGGAC 2 cut(s) 25, 503
FatI CATG 2 cut(s) 293, 573
FauI CCCGC 1 cut(s) 116
FblI GTMKAC 1 cut(s) 69
Fnu4HI GCNGC 3 cut(s) 228, 684, 687
FokI GGATG 5 cut(s) 128, 159, 581, 615, 621
Fsp4HI GCNGC 3 cut(s) 228, 684, 687
FspBI CTAG 1 cut(s) 675
GlaI GCGC 1 cut(s) 355
GluI GCNGC 3 cut(s) 228, 684, 687
HaeII RGCGCY 1 cut(s) 357
HapII CCGG 1 cut(s) 317
HgaI GACGC 1 cut(s) 590
HhaI GCGC 1 cut(s) 356
Hin1II CATG 2 cut(s) 297, 577
Hin6I GCGC 1 cut(s) 354
HinP1I GCGC 1 cut(s) 354
HinfI GANTC 3 cut(s) 13, 138, 421
HpaII CCGG 1 cut(s) 317
HphI GGTGA 3 cut(s) 8, 108, 524
Hpy166II GTNNAC 2 cut(s) 70, 414
Hpy188I TCNGA 4 cut(s) 343, 351, 426, 642
Hpy8I GTNNAC 2 cut(s) 70, 414
Hpy99I CGWCG 1 cut(s) 421
HpyAV CCTTC 1 cut(s) 202
HpyCH4III ACNGT 1 cut(s) 67
HpyCH4IV ACGT 1 cut(s) 553
HpyCH4V TGCA 4 cut(s) 257, 563, 620, 686
HpyF10VI GCNNNNNNNGC 5 cut(s) 36, 120, 533, 671, 680
HpyF3I CTNAG 2 cut(s) 40, 223
HpySE526I ACGT 1 cut(s) 553
Hsp92II CATG 2 cut(s) 297, 577
HspAI GCGC 1 cut(s) 354
LmnI GCTCC 2 cut(s) 111, 351
LpnPI CCDG 4 cut(s) 330, 475, 534, 606
Lsp1109I GCAGC 2 cut(s) 670, 698
LweI GCATC 3 cut(s) 64, 181, 593
MaeI CTAG 1 cut(s) 675
MaeII ACGT 1 cut(s) 553
MbiI CCGCTC 1 cut(s) 114
MboII GAAGA 3 cut(s) 52, 220, 561
MfeI CAATTG 2 cut(s) 48, 440
MhlI GDGCHC 1 cut(s) 237
MluCI AATT 9 cut(s) 48, 56, 83, 181, 211, 258, 440, 647, 701
MlyI GAGTC 2 cut(s) 7, 415
MmeI TCCRAC 1 cut(s) 366
MnlI CCTC 6 cut(s) 119, 324, 357, 526, 586, 648
MseI TTAA 4 cut(s) 447, 473, 506, 696
MspCI CTTAAG 1 cut(s) 446
MspI CCGG 1 cut(s) 317
MunI CAATTG 2 cut(s) 48, 440
MwoI GCNNNNNNNGC 5 cut(s) 36, 120, 533, 671, 680
NlaIII CATG 2 cut(s) 297, 577
NlaIV GGNNCC 1 cut(s) 128
NspI RCATGY 1 cut(s) 297
PciI ACATGT 1 cut(s) 293
PfeI GAWTC 1 cut(s) 138
PkrI GCNGC 3 cut(s) 229, 685, 688
PleI GAGTC 2 cut(s) 7, 415
PpsI GAGTC 2 cut(s) 7, 415
PscI ACATGT 1 cut(s) 293
PshBI ATTAAT 1 cut(s) 696
PspN4I GGNNCC 1 cut(s) 128
PstI CTGCAG 1 cut(s) 688
RsaI GTAC 1 cut(s) 494
RsaNI GTAC 1 cut(s) 493
SaqAI TTAA 4 cut(s) 447, 473, 506, 696
SatI GCNGC 3 cut(s) 228, 684, 687
SchI GAGTC 2 cut(s) 7, 415
SduI GDGCHC 1 cut(s) 237
SetI ASST 5 cut(s) 46, 494, 514, 556, 632
SfaNI GCATC 3 cut(s) 64, 181, 593
SfcI CTRYAG 2 cut(s) 66, 684
SmlI CTYRAG 1 cut(s) 446
SmoI CTYRAG 1 cut(s) 446
Sse9I AATT 9 cut(s) 48, 56, 83, 181, 211, 258, 440, 647, 701
SsiI CCGC 3 cut(s) 112, 123, 227
SspMI CTAG 1 cut(s) 675
TaaI ACNGT 1 cut(s) 67
TaiI ACGT 1 cut(s) 556
TaqI TCGA 1 cut(s) 393
TasI AATT 9 cut(s) 48, 56, 83, 181, 211, 258, 440, 647, 701
TauI GCSGC 1 cut(s) 230
TfiI GAWTC 1 cut(s) 138
Tru1I TTAA 4 cut(s) 447, 473, 506, 696
Tru9I TTAA 4 cut(s) 447, 473, 506, 696
TscAI CASTG 2 cut(s) 70, 237
TseI GCWGC 2 cut(s) 683, 686
TspDTI ATGAA 4 cut(s) 130, 221, 235, 590
TspRI CASTG 2 cut(s) 70, 237
Vha464I CTTAAG 1 cut(s) 446
VspI ATTAAT 1 cut(s) 696
XapI RAATTY 1 cut(s) 211
XceI RCATGY 1 cut(s) 297
XmiI GTMKAC 1 cut(s) 69
XspI CTAG 1 cut(s) 675
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.