Rw4G007920

Cell wall vacuolar inhibitor of fructosidase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0000921
Physical Location & Seq
Forward (+)
16997117 .. 16997467
351 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G007920.1

Sequence Viewer

Length: 351 bp
ATGGTCGATGTTGTTAAGGCTAAGGCAACTGATACTGTAAACCATATCAATGATCTTCTTAAACAGACATCGGGAGCTCAACCCTTAAGTTCTTGCCTCGATGTATATAATGTAGTTCTACAGGCTAAAGTCCCAAAAGCCGGTGAAGCTCTGTCTAAAGGTGACCCTAAGTTTGCATACCAAGGCGTGAATGACGTTGGCCTAGGGGCAGATTTATGTGAAAATAGTTTTTCGGGCAGCTCATCTCCTCTCACATATTTGAACAAAACTGTTAGGGATGTTACGGATATGGCTTCAGCAATTGTTCAGCATTTAGTTCCTCTTGTTTCTCTTTTCAACTATGTTAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

116

Amino Acids

12.33

Weight (kDa)

5.48

Isoelectric Point (pI)

29.15

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcuI CTGAAG 1 cut(s) 279
AfiI CCNNNNNNNGG 1 cut(s) 140
AflII CTTAAG 1 cut(s) 85
AgsI TTSAA 2 cut(s) 262, 337
AluBI AGCT 3 cut(s) 77, 149, 240
AluI AGCT 3 cut(s) 77, 149, 240
Alw21I GWGCWC 1 cut(s) 79
AoxI GGCC 1 cut(s) 199
ApeKI GCWGC 1 cut(s) 237
ArsI GACNNNNNNTTYG 2 cut(s) 155, 187
AspA2I CCTAGG 1 cut(s) 202
AsuHPI GGTGA 2 cut(s) 155, 173
AvrII CCTAGG 1 cut(s) 202
BanII GRGCYC 1 cut(s) 79
Bbv12I GWGCWC 1 cut(s) 79
BbvI GCAGC 1 cut(s) 249
BfaI CTAG 1 cut(s) 203
BfmI CTRYAG 1 cut(s) 119
BfrI CTTAAG 1 cut(s) 85
BisI GCNGC 1 cut(s) 238
BlnI CCTAGG 1 cut(s) 202
BlsI GCNGC 1 cut(s) 239
Bpu10I CCTNAGC 1 cut(s) 21
BsaJI CCNNGG 2 cut(s) 181, 202
Bsc4I CCNNNNNNNGG 1 cut(s) 140
Bse118I RCCGGY 1 cut(s) 140
BseDI CCNNGG 2 cut(s) 181, 202
BseGI GGATG 1 cut(s) 283
BseLI CCNNNNNNNGG 1 cut(s) 140
BseRI GAGGAG 1 cut(s) 237
BseXI GCAGC 1 cut(s) 249
BshFI GGCC 1 cut(s) 201
BsiHKAI GWGCWC 1 cut(s) 79
BsiSI CCGG 1 cut(s) 141
BslFI GGGAC 1 cut(s) 116
BslI CCNNNNNNNGG 1 cut(s) 140
BsmFI GGGAC 1 cut(s) 116
BsnI GGCC 1 cut(s) 201
Bsp1286I GDGCHC 1 cut(s) 79
Bsp143I GATC 1 cut(s) 52
BspANI GGCC 1 cut(s) 201
BspTI CTTAAG 1 cut(s) 85
BsrFI RCCGGY 1 cut(s) 140
BssAI RCCGGY 1 cut(s) 140
BssECI CCNNGG 2 cut(s) 181, 202
BssMI GATC 1 cut(s) 52
BssT1I CCWWGG 2 cut(s) 181, 202
Bst4CI ACNGT 2 cut(s) 37, 271
BstAFI CTTAAG 1 cut(s) 85
BstDEI CTNAG 2 cut(s) 21, 168
BstEII GGTNACC 1 cut(s) 161
BstF5I GGATG 1 cut(s) 283
BstKTI GATC 1 cut(s) 55
BstMBI GATC 1 cut(s) 52
BstMWI GCNNNNNNNGC 1 cut(s) 146
BstPI GGTNACC 1 cut(s) 161
BstSFI CTRYAG 1 cut(s) 119
BstV1I GCAGC 1 cut(s) 249
BsuRI GGCC 1 cut(s) 201
BtsCI GGATG 1 cut(s) 283
Cfr10I RCCGGY 1 cut(s) 140
CviJI RGCY 8 cut(s) 20, 77, 125, 140, 149, 201, 240, 293
CviKI_1 RGCY 8 cut(s) 20, 77, 125, 140, 149, 201, 240, 293
DdeI CTNAG 2 cut(s) 21, 168
DpnI GATC 1 cut(s) 54
DpnII GATC 1 cut(s) 52
Ecl136II GAGCTC 1 cut(s) 77
Eco130I CCWWGG 2 cut(s) 181, 202
Eco24I GRGCYC 1 cut(s) 79
Eco53kI GAGCTC 1 cut(s) 77
Eco57I CTGAAG 1 cut(s) 279
Eco91I GGTNACC 1 cut(s) 161
EcoICRI GAGCTC 1 cut(s) 77
EcoO65I GGTNACC 1 cut(s) 161
EcoT14I CCWWGG 2 cut(s) 181, 202
EcoT38I GRGCYC 1 cut(s) 79
ErhI CCWWGG 2 cut(s) 181, 202
FaiI YATR 8 cut(s) 45, 106, 108, 178, 217, 256, 290, 342
FaqI GGGAC 1 cut(s) 116
Fnu4HI GCNGC 1 cut(s) 238
FokI GGATG 1 cut(s) 290
FriOI GRGCYC 1 cut(s) 79
Fsp4HI GCNGC 1 cut(s) 238
FspBI CTAG 1 cut(s) 203
GluI GCNGC 1 cut(s) 238
HaeIII GGCC 1 cut(s) 201
HapII CCGG 1 cut(s) 141
HpaII CCGG 1 cut(s) 141
HphI GGTGA 2 cut(s) 155, 173
Hpy166II GTNNAC 1 cut(s) 40
Hpy188III TCNNGA 1 cut(s) 72
Hpy8I GTNNAC 1 cut(s) 40
HpyCH4III ACNGT 2 cut(s) 37, 271
HpyCH4IV ACGT 1 cut(s) 195
HpyCH4V TGCA 1 cut(s) 176
HpyF10VI GCNNNNNNNGC 1 cut(s) 146
HpyF3I CTNAG 2 cut(s) 21, 168
HpySE526I ACGT 1 cut(s) 195
Kzo9I GATC 1 cut(s) 52
LmnI GCTCC 1 cut(s) 74
LpnPI CCDG 2 cut(s) 107, 154
Lsp1109I GCAGC 1 cut(s) 249
MaeI CTAG 1 cut(s) 203
MaeII ACGT 1 cut(s) 195
MaeIII GTNAC 2 cut(s) 161, 280
MalI GATC 1 cut(s) 54
MboI GATC 1 cut(s) 52
MboII GAAGA 1 cut(s) 47
MfeI CAATTG 1 cut(s) 300
MhlI GDGCHC 1 cut(s) 79
MluCI AATT 2 cut(s) 300, 346
MnlI CCTC 3 cut(s) 107, 258, 330
MseI TTAA 5 cut(s) 15, 60, 86, 345, 349
MslI CAYNNNNRTG 1 cut(s) 48
MspCI CTTAAG 1 cut(s) 85
MspI CCGG 1 cut(s) 141
MunI CAATTG 1 cut(s) 300
MwoI GCNNNNNNNGC 1 cut(s) 146
NdeII GATC 1 cut(s) 52
NmuCI GTSAC 1 cut(s) 161
PacI TTAATTAA 1 cut(s) 349
PkrI GCNGC 1 cut(s) 239
Psp124BI GAGCTC 1 cut(s) 79
PspEI GGTNACC 1 cut(s) 161
RseI CAYNNNNRTG 1 cut(s) 48
SacI GAGCTC 1 cut(s) 79
SaqAI TTAA 5 cut(s) 15, 60, 86, 345, 349
SatI GCNGC 1 cut(s) 238
Sau3AI GATC 1 cut(s) 52
SduI GDGCHC 1 cut(s) 79
SetI ASST 5 cut(s) 79, 151, 163, 198, 242
SfcI CTRYAG 1 cut(s) 119
SmiMI CAYNNNNRTG 1 cut(s) 48
SmlI CTYRAG 1 cut(s) 85
SmoI CTYRAG 1 cut(s) 85
Sse9I AATT 2 cut(s) 300, 346
SspMI CTAG 1 cut(s) 203
SstI GAGCTC 1 cut(s) 79
StyI CCWWGG 2 cut(s) 181, 202
TaaI ACNGT 2 cut(s) 37, 271
TaiI ACGT 1 cut(s) 198
TaqI TCGA 2 cut(s) 6, 99
TasI AATT 2 cut(s) 300, 346
Tru1I TTAA 5 cut(s) 15, 60, 86, 345, 349
Tru9I TTAA 5 cut(s) 15, 60, 86, 345, 349
TseFI GTSAC 1 cut(s) 161
TseI GCWGC 1 cut(s) 237
Tsp45I GTSAC 1 cut(s) 161
TspGWI ACGGA 1 cut(s) 299
Vha464I CTTAAG 1 cut(s) 85
XmaJI CCTAGG 1 cut(s) 202
XspI CTAG 1 cut(s) 203
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.