Rh4BG274400

Cell wall vacuolar inhibitor of fructosidase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0000921
Physical Location & Seq
Forward (+)
44854096 .. 44854629
534 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG274400.1

Sequence Viewer

Length: 534 bp
ATGAAGAGCTTAATATCTCTAGCAAAACTCATCATACTTGTCAGTCAAATTGTGTTTCTTCCAATAAGCCAAGGTAGCAAGGATTTTTGGCCAATGGATGCTAAACTCATTGATGAAACATGTCAAAAAACCCCAAACCCCAATCTTTGTGTCTCATCTCTCAAATCAGACCCTCGAAGTTCCGATGCTGATGTCAAAGGCTTGGGCGTAATAATGGTGGATGTGGTTAAGGCAAAAGCGATTATCGGTCAAAACAAAGCACAAGAATTGCTTAAGCAGAGCCCAGGAGATCTACGCTTAAAAGTTTGTTCAGGTGACTATGATACAATATCAAATCATCATGTCCCAAATGCTTTACTGGCATTTAAGAAAGATGCCCCGGACATTGCTGAAGAAGAAATGAGTGAAGCTGCTGAAGAGGCAAGGGCATGTGAGTCTAGTTTTTCAGGGGGTTCTCCTCTGACAGATGTGAATAATGTTTTAGCAGATGTTGCGGCTATTACTGCAGCTATAGCAAAGGAATTAGTTGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

177

Amino Acids

18.81

Weight (kDa)

4.93

Isoelectric Point (pI)

38.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 35 - 165 6e-18 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 494
AcoI YGGCCR 1 cut(s) 89
AcuI CTGAAG 2 cut(s) 411, 435
AflII CTTAAG 1 cut(s) 272
AflIII ACRYGT 1 cut(s) 119
AjnI CCWGG 1 cut(s) 283
AluBI AGCT 3 cut(s) 9, 410, 509
AluI AGCT 3 cut(s) 9, 410, 509
Alw26I GTCTC 1 cut(s) 157
AoxI GGCC 1 cut(s) 89
ApeKI GCWGC 2 cut(s) 410, 506
AsuC2I CCSGG 1 cut(s) 380
AsuHPI GGTGA 1 cut(s) 326
BalI TGGCCA 1 cut(s) 91
BanII GRGCYC 1 cut(s) 284
BbvI GCAGC 2 cut(s) 397, 518
BciT130I CCWGG 1 cut(s) 285
BcnI CCSGG 1 cut(s) 380
BcoDI GTCTC 1 cut(s) 157
BfaI CTAG 2 cut(s) 20, 438
BfmI CTRYAG 2 cut(s) 504, 510
BfrI CTTAAG 1 cut(s) 272
BglII AGATCT 1 cut(s) 289
BisI GCNGC 3 cut(s) 411, 495, 507
BlsI GCNGC 3 cut(s) 412, 496, 508
Bme1390I CCNGG 2 cut(s) 285, 380
BmrFI CCNGG 2 cut(s) 285, 380
BmsI GCATC 3 cut(s) 88, 175, 364
BpuMI CCSGG 1 cut(s) 380
BsaJI CCNNGG 3 cut(s) 70, 283, 378
Bse1I ACTGG 1 cut(s) 363
Bse3DI GCAATG 1 cut(s) 384
BseBI CCWGG 1 cut(s) 285
BseDI CCNNGG 3 cut(s) 70, 283, 378
BseGI GGATG 2 cut(s) 103, 226
BseMI GCAATG 1 cut(s) 384
BseNI ACTGG 1 cut(s) 363
BseRI GAGGAG 1 cut(s) 447
BseXI GCAGC 2 cut(s) 397, 518
BshFI GGCC 1 cut(s) 91
BsiSI CCGG 1 cut(s) 380
BslFI GGGAC 1 cut(s) 329
BsmAI GTCTC 1 cut(s) 157
BsmFI GGGAC 1 cut(s) 329
BsnI GGCC 1 cut(s) 91
Bsp1286I GDGCHC 1 cut(s) 284
Bsp143I GATC 1 cut(s) 289
BspACI CCGC 1 cut(s) 494
BspANI GGCC 1 cut(s) 91
BspMAI CTGCAG 1 cut(s) 508
BspTI CTTAAG 1 cut(s) 272
BsrDI GCAATG 1 cut(s) 384
BsrI ACTGG 1 cut(s) 363
BssECI CCNNGG 3 cut(s) 70, 283, 378
BssMI GATC 1 cut(s) 289
BssT1I CCWWGG 1 cut(s) 70
Bst2UI CCWGG 1 cut(s) 285
Bst6I CTCTTC 1 cut(s) 411
BstAFI CTTAAG 1 cut(s) 272
BstAPI GCANNNNNTGC 1 cut(s) 491
BstF5I GGATG 2 cut(s) 103, 226
BstKTI GATC 1 cut(s) 292
BstMAI GTCTC 1 cut(s) 157
BstMBI GATC 1 cut(s) 289
BstMWI GCNNNNNNNGC 6 cut(s) 75, 359, 419, 491, 503, 512
BstNI CCWGG 1 cut(s) 285
BstNSI RCATGY 2 cut(s) 123, 432
BstSCI CCNGG 2 cut(s) 283, 378
BstSFI CTRYAG 2 cut(s) 504, 510
BstV1I GCAGC 2 cut(s) 397, 518
BstX2I RGATCY 1 cut(s) 289
BstYI RGATCY 1 cut(s) 289
BsuRI GGCC 1 cut(s) 91
BtsCI GGATG 2 cut(s) 103, 226
CviAII CATG 3 cut(s) 120, 341, 429
CviJI RGCY 8 cut(s) 9, 69, 91, 201, 282, 410, 497, 509
CviKI_1 RGCY 8 cut(s) 9, 69, 91, 201, 282, 410, 497, 509
DpnI GATC 1 cut(s) 291
DpnII GATC 1 cut(s) 289
EaeI YGGCCR 1 cut(s) 89
Eam1104I CTCTTC 1 cut(s) 411
EarI CTCTTC 1 cut(s) 411
Eco130I CCWWGG 1 cut(s) 70
Eco24I GRGCYC 1 cut(s) 284
Eco57I CTGAAG 2 cut(s) 411, 435
EcoRII CCWGG 1 cut(s) 283
EcoT14I CCWWGG 1 cut(s) 70
EcoT38I GRGCYC 1 cut(s) 284
ErhI CCWWGG 1 cut(s) 70
FaeI CATG 3 cut(s) 123, 344, 432
FaiI YATR 6 cut(s) 35, 121, 321, 342, 430, 512
FalI AAGNNNNNCTT 2 cut(s) 255, 287
FaqI GGGAC 1 cut(s) 329
FatI CATG 3 cut(s) 119, 340, 428
Fnu4HI GCNGC 3 cut(s) 411, 495, 507
FokI GGATG 2 cut(s) 110, 233
FriOI GRGCYC 1 cut(s) 284
Fsp4HI GCNGC 3 cut(s) 411, 495, 507
FspBI CTAG 2 cut(s) 20, 438
GluI GCNGC 3 cut(s) 411, 495, 507
HaeIII GGCC 1 cut(s) 91
HapII CCGG 1 cut(s) 380
Hin1II CATG 3 cut(s) 123, 344, 432
HinfI GANTC 1 cut(s) 434
HpaII CCGG 1 cut(s) 380
HphI GGTGA 1 cut(s) 326
Hpy188I TCNGA 3 cut(s) 169, 184, 462
HpyCH4V TGCA 1 cut(s) 506
HpyF10VI GCNNNNNNNGC 6 cut(s) 75, 359, 419, 491, 503, 512
Hsp92II CATG 3 cut(s) 123, 344, 432
Kzo9I GATC 1 cut(s) 289
LpnPI CCDG 6 cut(s) 270, 297, 297, 344, 393, 432
Lsp1109I GCAGC 2 cut(s) 397, 518
LweI GCATC 3 cut(s) 88, 175, 364
MaeI CTAG 2 cut(s) 20, 438
MaeIII GTNAC 1 cut(s) 314
MalI GATC 1 cut(s) 291
MboI GATC 1 cut(s) 289
MboII GAAGA 5 cut(s) 16, 50, 404, 407, 428
MflI RGATCY 1 cut(s) 289
MhlI GDGCHC 1 cut(s) 284
MlsI TGGCCA 1 cut(s) 91
MluCI AATT 3 cut(s) 48, 266, 521
MluNI TGGCCA 1 cut(s) 91
MlyI GAGTC 1 cut(s) 443
MnlI CCTC 3 cut(s) 183, 412, 468
Mox20I TGGCCA 1 cut(s) 91
MscI TGGCCA 1 cut(s) 91
MseI TTAA 5 cut(s) 11, 228, 273, 299, 366
Msp20I TGGCCA 1 cut(s) 91
MspCI CTTAAG 1 cut(s) 272
MspI CCGG 1 cut(s) 380
MspR9I CCNGG 2 cut(s) 285, 380
MvaI CCWGG 1 cut(s) 285
MwoI GCNNNNNNNGC 6 cut(s) 75, 359, 419, 491, 503, 512
NciI CCSGG 1 cut(s) 380
NdeII GATC 1 cut(s) 289
NlaIII CATG 3 cut(s) 123, 344, 432
NmuCI GTSAC 1 cut(s) 314
NspI RCATGY 2 cut(s) 123, 432
PciI ACATGT 1 cut(s) 119
PkrI GCNGC 3 cut(s) 412, 496, 508
PleI GAGTC 1 cut(s) 442
PpsI GAGTC 1 cut(s) 442
PscI ACATGT 1 cut(s) 119
Psp6I CCWGG 1 cut(s) 283
PspGI CCWGG 1 cut(s) 283
PstI CTGCAG 1 cut(s) 508
PsuI RGATCY 1 cut(s) 289
SaqAI TTAA 5 cut(s) 11, 228, 273, 299, 366
SatI GCNGC 3 cut(s) 411, 495, 507
Sau3AI GATC 1 cut(s) 289
SchI GAGTC 1 cut(s) 443
ScrFI CCNGG 2 cut(s) 285, 380
SduI GDGCHC 1 cut(s) 284
SetI ASST 5 cut(s) 11, 76, 316, 412, 511
SfaNI GCATC 3 cut(s) 88, 175, 364
SfcI CTRYAG 2 cut(s) 504, 510
SmlI CTYRAG 1 cut(s) 272
SmoI CTYRAG 1 cut(s) 272
Sse9I AATT 3 cut(s) 48, 266, 521
SsiI CCGC 1 cut(s) 494
SspMI CTAG 2 cut(s) 20, 438
StyD4I CCNGG 2 cut(s) 283, 378
StyI CCWWGG 1 cut(s) 70
TaqI TCGA 1 cut(s) 175
TaqII GACCGA 1 cut(s) 236
TasI AATT 3 cut(s) 48, 266, 521
TauI GCSGC 1 cut(s) 497
Tru1I TTAA 5 cut(s) 11, 228, 273, 299, 366
Tru9I TTAA 5 cut(s) 11, 228, 273, 299, 366
TseFI GTSAC 1 cut(s) 314
TseI GCWGC 2 cut(s) 410, 506
Tsp45I GTSAC 1 cut(s) 314
TspDTI ATGAA 2 cut(s) 17, 129
Vha464I CTTAAG 1 cut(s) 272
XceI RCATGY 2 cut(s) 123, 432
XspI CTAG 2 cut(s) 20, 438
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.