MD16G1150100.v1.1

Cell wall vacuolar inhibitor of fructosidase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0000921
Physical Location & Seq
Forward (+)
11835878 .. 11836402
525 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1150100.v1.1.491

Sequence Viewer

Length: 525 bp
ATGAAGAATTTAATCCCTCTAGCACTCACTTTTCTCATACAAATTTCCCTTCTTCCGGTAAGCCAATGCAGGCCGGTTTACGTGCCAAATGACGCCACTCTTATTGACCAAACATGCCAAAAAACACCGGATCACAACCTTTGCGTCTCTTCTCTTCGATCAGACCCTCGAAGCTCCAGTGCAGACGTCAAAGGCTTAGGCATCATAATGGTTGATGTAGTTAAGGGTAAGGCAACGGACACATTGAACCAAATTAATGACCTTCTTAAGCAGAGGCCTGGTGACAAATCATTGAGCTCTTGTGCCGAAGTGTACAACACAGTTATAACAGCTGATGTGCCGGAAGCCAGTCAGGGTTTTAGTTTGGGTAACCCTAAGTTTGCGGAGCAAGGCATGAATGACGCTGCCGGCGAGGCTGTTTCGTGCGAAAATGGTTTTTCGGGGAGCTCTCCTCTGACGGATAAAAACAAATCTGTTCATGATGTTGCCGCTGTGGCTGCTGCAATTGCCAAAACTTTAGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

175

Amino Acids

18.35

Weight (kDa)

5.14

Isoelectric Point (pI)

32.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 34 - 169 2.7e-20 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 326
AatII GACGTC 1 cut(s) 189
AciI CCGC 2 cut(s) 383, 489
AclWI GGATC 1 cut(s) 138
AcsI RAATTY 2 cut(s) 7, 42
AcyI GRCGYC 2 cut(s) 93, 186
AfaI GTAC 1 cut(s) 314
AfiI CCNNNNNNNGG 1 cut(s) 55
AflII CTTAAG 1 cut(s) 266
AgsI TTSAA 1 cut(s) 247
AjnI CCWGG 1 cut(s) 277
AluBI AGCT 4 cut(s) 174, 297, 332, 447
AluI AGCT 4 cut(s) 174, 297, 332, 447
Alw21I GWGCWC 2 cut(s) 299, 449
Alw26I GTCTC 1 cut(s) 151
AlwI GGATC 1 cut(s) 138
AoxI GGCC 2 cut(s) 71, 275
ApeKI GCWGC 3 cut(s) 404, 497, 500
ApoI RAATTY 2 cut(s) 7, 42
AseI ATTAAT 1 cut(s) 255
AsuHPI GGTGA 1 cut(s) 293
BanII GRGCYC 2 cut(s) 299, 449
Bbv12I GWGCWC 2 cut(s) 299, 449
BbvI GCAGC 3 cut(s) 391, 484, 487
BciT130I CCWGG 1 cut(s) 279
BcoDI GTCTC 1 cut(s) 151
BfaI CTAG 1 cut(s) 20
BfrI CTTAAG 1 cut(s) 266
BglI GCCNNNNNGGC 2 cut(s) 413, 494
BisI GCNGC 4 cut(s) 405, 489, 498, 501
BlsI GCNGC 4 cut(s) 406, 490, 499, 502
Bme1390I CCNGG 1 cut(s) 279
BmrFI CCNGG 1 cut(s) 279
BmsI GCATC 1 cut(s) 210
BplI GAGNNNNNCTC 2 cut(s) 436, 468
BpmI CTGGAG 1 cut(s) 160
Bpu10I CCTNAGC 1 cut(s) 196
BsaAI YACGTR 1 cut(s) 82
BsaHI GRCGYC 2 cut(s) 93, 186
BsaWI WCCGGW 2 cut(s) 55, 127
Bsc4I CCNNNNNNNGG 1 cut(s) 55
Bse118I RCCGGY 2 cut(s) 73, 407
Bse1I ACTGG 2 cut(s) 177, 348
BseBI CCWGG 1 cut(s) 279
BseLI CCNNNNNNNGG 1 cut(s) 55
BseNI ACTGG 2 cut(s) 177, 348
BseRI GAGGAG 1 cut(s) 441
BseXI GCAGC 3 cut(s) 391, 484, 487
BsgI GTGCAG 1 cut(s) 201
BshFI GGCC 2 cut(s) 73, 277
BsiHKAI GWGCWC 2 cut(s) 299, 449
BsiSI CCGG 5 cut(s) 56, 74, 128, 341, 408
BslI CCNNNNNNNGG 1 cut(s) 55
BsmAI GTCTC 1 cut(s) 151
BsmBI CGTCTC 1 cut(s) 151
BsnI GGCC 2 cut(s) 73, 277
Bsp1286I GDGCHC 2 cut(s) 299, 449
Bsp1407I TGTACA 1 cut(s) 312
Bsp143I GATC 2 cut(s) 130, 158
BspACI CCGC 2 cut(s) 383, 489
BspANI GGCC 2 cut(s) 73, 277
BspHI TCATGA 1 cut(s) 478
BspPI GGATC 1 cut(s) 138
BspTI CTTAAG 1 cut(s) 266
BsrFI RCCGGY 2 cut(s) 73, 407
BsrGI TGTACA 1 cut(s) 312
BsrI ACTGG 2 cut(s) 177, 348
BssAI RCCGGY 2 cut(s) 73, 407
BssMI GATC 2 cut(s) 130, 158
BssNI GRCGYC 2 cut(s) 93, 186
Bst2UI CCWGG 1 cut(s) 279
Bst4CI ACNGT 1 cut(s) 322
Bst6I CTCTTC 2 cut(s) 154, 159
BstACI GRCGYC 2 cut(s) 93, 186
BstAFI CTTAAG 1 cut(s) 266
BstAUI TGTACA 1 cut(s) 312
BstBAI YACGTR 1 cut(s) 82
BstC8I GCNNGC 2 cut(s) 71, 409
BstDEI CTNAG 2 cut(s) 196, 375
BstEII GGTNACC 1 cut(s) 368
BstKTI GATC 2 cut(s) 133, 161
BstMAI GTCTC 1 cut(s) 151
BstMBI GATC 2 cut(s) 130, 158
BstMWI GCNNNNNNNGC 4 cut(s) 413, 494, 497, 506
BstNI CCWGG 1 cut(s) 279
BstNSI RCATGY 1 cut(s) 117
BstPI GGTNACC 1 cut(s) 368
BstSCI CCNGG 1 cut(s) 277
BstV1I GCAGC 3 cut(s) 391, 484, 487
BsuRI GGCC 2 cut(s) 73, 277
BtsIMutI CAGTG 1 cut(s) 184
Cac8I GCNNGC 2 cut(s) 71, 409
CciI TCATGA 1 cut(s) 478
Cfr10I RCCGGY 2 cut(s) 73, 407
CseI GACGC 3 cut(s) 101, 133, 410
Csp6I GTAC 1 cut(s) 313
CspCI CAANNNNNGTGG 2 cut(s) 85, 120
CviAII CATG 3 cut(s) 114, 394, 479
CviQI GTAC 1 cut(s) 313
DdeI CTNAG 2 cut(s) 196, 375
DpnI GATC 2 cut(s) 132, 160
DpnII GATC 2 cut(s) 130, 158
Eam1104I CTCTTC 2 cut(s) 154, 159
EarI CTCTTC 2 cut(s) 154, 159
Ecl136II GAGCTC 2 cut(s) 297, 447
Eco147I AGGCCT 1 cut(s) 277
Eco24I GRGCYC 2 cut(s) 299, 449
Eco53kI GAGCTC 2 cut(s) 297, 447
Eco91I GGTNACC 1 cut(s) 368
EcoICRI GAGCTC 2 cut(s) 297, 447
EcoO65I GGTNACC 1 cut(s) 368
EcoRII CCWGG 1 cut(s) 277
EcoT38I GRGCYC 2 cut(s) 299, 449
Esp3I CGTCTC 1 cut(s) 151
FaeI CATG 3 cut(s) 117, 397, 482
FaiI YATR 6 cut(s) 38, 115, 206, 326, 395, 480
FatI CATG 3 cut(s) 113, 393, 478
Fnu4HI GCNGC 4 cut(s) 405, 489, 498, 501
FriOI GRGCYC 2 cut(s) 299, 449
Fsp4HI GCNGC 4 cut(s) 405, 489, 498, 501
FspBI CTAG 1 cut(s) 20
GluI GCNGC 4 cut(s) 405, 489, 498, 501
GsuI CTGGAG 1 cut(s) 160
HaeIII GGCC 2 cut(s) 73, 277
HapII CCGG 5 cut(s) 56, 74, 128, 341, 408
HgaI GACGC 3 cut(s) 101, 133, 410
Hin1I GRCGYC 2 cut(s) 93, 186
Hin1II CATG 3 cut(s) 117, 397, 482
HpaII CCGG 5 cut(s) 56, 74, 128, 341, 408
HphI GGTGA 1 cut(s) 293
Hpy166II GTNNAC 2 cut(s) 79, 313
Hpy188I TCNGA 2 cut(s) 163, 456
Hpy188III TCNNGA 1 cut(s) 479
Hpy8I GTNNAC 2 cut(s) 79, 313
HpyAV CCTTC 2 cut(s) 59, 272
HpyCH4III ACNGT 1 cut(s) 322
HpyCH4IV ACGT 2 cut(s) 81, 186
HpyCH4V TGCA 3 cut(s) 69, 182, 503
HpyF10VI GCNNNNNNNGC 4 cut(s) 413, 494, 497, 506
HpyF3I CTNAG 2 cut(s) 196, 375
HpySE526I ACGT 2 cut(s) 81, 186
Hsp92I GRCGYC 2 cut(s) 93, 186
Hsp92II CATG 3 cut(s) 117, 397, 482
KroI GCCGGC 1 cut(s) 407
KroNI GCCGGC 1 cut(s) 409
Kzo9I GATC 2 cut(s) 130, 158
LmnI GCTCC 3 cut(s) 179, 385, 444
Lsp1109I GCAGC 3 cut(s) 391, 484, 487
LweI GCATC 1 cut(s) 210
MaeI CTAG 1 cut(s) 20
MaeII ACGT 2 cut(s) 81, 186
MaeIII GTNAC 2 cut(s) 281, 368
MalI GATC 2 cut(s) 132, 160
MboI GATC 2 cut(s) 130, 158
MboII GAAGA 4 cut(s) 16, 44, 141, 146
MfeI CAATTG 1 cut(s) 504
MhlI GDGCHC 2 cut(s) 299, 449
MluCI AATT 4 cut(s) 7, 42, 252, 504
MnlI CCTC 5 cut(s) 27, 177, 267, 406, 462
MroNI GCCGGC 1 cut(s) 407
MseI TTAA 4 cut(s) 11, 222, 255, 267
MslI CAYNNNNRTG 1 cut(s) 206
MspA1I CMGCKG 2 cut(s) 332, 491
MspCI CTTAAG 1 cut(s) 266
MspI CCGG 5 cut(s) 56, 74, 128, 341, 408
MspR9I CCNGG 1 cut(s) 279
MunI CAATTG 1 cut(s) 504
MvaI CCWGG 1 cut(s) 279
MwoI GCNNNNNNNGC 4 cut(s) 413, 494, 497, 506
NaeI GCCGGC 1 cut(s) 409
NdeII GATC 2 cut(s) 130, 158
NgoMIV GCCGGC 1 cut(s) 407
NlaIII CATG 3 cut(s) 117, 397, 482
NmuCI GTSAC 1 cut(s) 281
NspI RCATGY 1 cut(s) 117
PagI TCATGA 1 cut(s) 478
PceI AGGCCT 1 cut(s) 277
PcsI WCGNNNNNNNCGW 1 cut(s) 408
PdiI GCCGGC 1 cut(s) 409
PkrI GCNGC 4 cut(s) 406, 490, 499, 502
Ppu21I YACGTR 1 cut(s) 82
PshBI ATTAAT 1 cut(s) 255
PsiI TTATAA 1 cut(s) 326
Psp124BI GAGCTC 2 cut(s) 299, 449
Psp6I CCWGG 1 cut(s) 277
PspEI GGTNACC 1 cut(s) 368
PspGI CCWGG 1 cut(s) 277
PvuII CAGCTG 1 cut(s) 332
RsaI GTAC 1 cut(s) 314
RsaNI GTAC 1 cut(s) 313
RseI CAYNNNNRTG 1 cut(s) 206
SacI GAGCTC 2 cut(s) 299, 449
SaqAI TTAA 4 cut(s) 11, 222, 255, 267
SatI GCNGC 4 cut(s) 405, 489, 498, 501
Sau3AI GATC 2 cut(s) 130, 158
ScrFI CCNGG 1 cut(s) 279
SduI GDGCHC 2 cut(s) 299, 449
SetI ASST 8 cut(s) 84, 141, 176, 189, 264, 299, 334, 449
SfaNI GCATC 1 cut(s) 210
SmiMI CAYNNNNRTG 1 cut(s) 206
SmlI CTYRAG 1 cut(s) 266
SmoI CTYRAG 1 cut(s) 266
Sse9I AATT 4 cut(s) 7, 42, 252, 504
SseBI AGGCCT 1 cut(s) 277
SsiI CCGC 2 cut(s) 383, 489
SspMI CTAG 1 cut(s) 20
SstI GAGCTC 2 cut(s) 299, 449
StuI AGGCCT 1 cut(s) 277
StyD4I CCNGG 1 cut(s) 277
TaaI ACNGT 1 cut(s) 322
TaiI ACGT 2 cut(s) 84, 189
TaqI TCGA 2 cut(s) 157, 169
TasI AATT 4 cut(s) 7, 42, 252, 504
TatI WGTACW 1 cut(s) 312
TauI GCSGC 1 cut(s) 491
Tru1I TTAA 4 cut(s) 11, 222, 255, 267
Tru9I TTAA 4 cut(s) 11, 222, 255, 267
TscAI CASTG 1 cut(s) 184
TseFI GTSAC 1 cut(s) 281
TseI GCWGC 3 cut(s) 404, 497, 500
Tsp45I GTSAC 1 cut(s) 281
TspDTI ATGAA 3 cut(s) 17, 410, 467
TspGWI ACGGA 2 cut(s) 251, 473
TspRI CASTG 1 cut(s) 184
Vha464I CTTAAG 1 cut(s) 266
VspI ATTAAT 1 cut(s) 255
XapI RAATTY 2 cut(s) 7, 42
XceI RCATGY 1 cut(s) 117
XspI CTAG 1 cut(s) 20
ZraI GACGTC 1 cut(s) 187
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.