AT1G49470

Family of unknown function (DUF716)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0001745
Physical Location & Seq
Forward (+)
18310428 .. 18311714
1287 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G49470.1

Sequence Viewer

Length: 909 bp
ATGGGAACGTTCTTAGGACACTTTGTGCCTGGTCTCTCACTTGCTCTTCTTGGTCTTTGGCATCTCTTCAACACCATAAGATCTTACTGTCTTAAAGGCCCTGAAGCTTTCTCTGCAAAATTCTGGTTCCCTTTCCCTAAACTCAAACATCTTGAGCTTATCTTGATTCTCTTCTTCTCCTTCCTCTCCATCACTCTCCTCACCCTAGATTTCCCTAATTTCAACTTCTCCTCTCTCAAACCAGACAACCTAGAACATGCTTCCATGTTCCTCCACCTCATTATCTTTGCGTGTTTCGCTCTCTTCTGCGAGCTAACCCTTTGCTCAGATCTCTTCTCCGGTCTAATCGGAGTTCTTTCTGCCTCTGTCTTCGCTCAAGAACTCTTCCTTCTTCATTTTCATTCCACTGATCATTCAGGTCTTGAAGGTCACTACCATTTCCTTCTCCAACTCATTGCTTTCGTCTCCTTCTCCTCGGCGCTAGCTTCTGCTTCGTTCCCTAAATCCTTCTCTGCGGCTCTATTCCTCCCGATATCGGTTATGTTTCAAGGATGCTGGTTCTTGAACATGGGGTTCATGCTTTGGATCCCAGAGTATGTTCCTAGAGGTTGCGTGAGTAACATGTCAACATCTACAGACAATAATAGAAGGAGTGTTTATCATAGTGGTGCGGTGGCTTGTGAGTCCCCTGGTGCTGAGATTAGGGCAAAAGCATTGGCGAATCTGCAGTTTAGTTGGATGTTGTCTGCTATACTTATCATTACATGTGCCTTGTGCCTCAAGTATTCCGGTAAAGTTGTGTTGCCTAAGATTAGGTCATCGTTGGAGTACGAGCGTCTTTGCCGGCAAGGAAGTGATCGTTCTGCAACCGTAACGGTGGAAGCACCGGCCAGTTCCGATCAACAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF716

Protein Analysis

302

Amino Acids

33.62

Weight (kDa)

7.14

Isoelectric Point (pI)

40.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF716 PF04819 114 - 259 1.2e-44 Family of unknown function (DUF716)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 515, 671
AclI AACGTT 1 cut(s) 8
AclWI GGATC 2 cut(s) 580, 593
AcoI YGGCCR 1 cut(s) 888
AcsI RAATTY 1 cut(s) 119
AcuI CTGAAG 1 cut(s) 123
AdeI CACNNNGTG 1 cut(s) 25
AfaI GTAC 1 cut(s) 830
AfiI CCNNNNNNNGG 1 cut(s) 535
AflIII ACRYGT 2 cut(s) 621, 764
AgsI TTSAA 5 cut(s) 70, 223, 425, 548, 565
AjnI CCWGG 2 cut(s) 28, 688
AluBI AGCT 4 cut(s) 107, 157, 313, 485
AluI AGCT 4 cut(s) 107, 157, 313, 485
Alw26I GTCTC 2 cut(s) 38, 469
AlwI GGATC 2 cut(s) 580, 593
AoxI GGCC 2 cut(s) 97, 888
ApoI RAATTY 1 cut(s) 119
AspLEI GCGC 1 cut(s) 481
AspS9I GGNCC 1 cut(s) 98
AsuHPI GGTGA 1 cut(s) 193
AsuNHI GCTAGC 1 cut(s) 481
BamHI GGATCC 1 cut(s) 585
BbsI GAAGAC 1 cut(s) 361
BccI CCATC 1 cut(s) 197
BciT130I CCWGG 2 cut(s) 30, 690
BclI TGATCA 1 cut(s) 409
BcoDI GTCTC 2 cut(s) 38, 469
BfaI CTAG 4 cut(s) 206, 251, 482, 603
BfmI CTRYAG 2 cut(s) 633, 725
BfoI RGCGCY 1 cut(s) 482
BglII AGATCT 2 cut(s) 80, 328
BisI GCNGC 1 cut(s) 516
BlsI GCNGC 1 cut(s) 517
Bme1390I CCNGG 2 cut(s) 30, 690
BmgT120I GGNCC 1 cut(s) 98
BmiI GGNNCC 2 cut(s) 128, 587
BmrFI CCNGG 2 cut(s) 30, 690
BmsI GCATC 2 cut(s) 70, 542
BmtI GCTAGC 1 cut(s) 485
BpiI GAAGAC 1 cut(s) 361
BpuEI CTTGAG 3 cut(s) 173, 360, 764
BsaI GGTCTC 1 cut(s) 38
BsaJI CCNNGG 2 cut(s) 474, 688
BsaWI WCCGGW 2 cut(s) 338, 788
Bsc4I CCNNNNNNNGG 1 cut(s) 535
Bse118I RCCGGY 2 cut(s) 843, 886
Bse1I ACTGG 1 cut(s) 891
Bse3DI GCAATG 1 cut(s) 453
BseBI CCWGG 2 cut(s) 30, 690
BseDI CCNNGG 2 cut(s) 474, 688
BseGI GGATG 2 cut(s) 557, 744
BseLI CCNNNNNNNGG 1 cut(s) 535
BseMI GCAATG 1 cut(s) 453
BseMII CTCAG 2 cut(s) 339, 687
BseNI ACTGG 1 cut(s) 891
BseRI GAGGAG 3 cut(s) 188, 220, 463
BshFI GGCC 2 cut(s) 99, 890
BsiSI CCGG 4 cut(s) 339, 789, 844, 887
BslFI GGGAC 1 cut(s) 670
BslI CCNNNNNNNGG 1 cut(s) 535
BsmAI GTCTC 2 cut(s) 38, 469
BsmBI CGTCTC 1 cut(s) 469
BsmFI GGGAC 1 cut(s) 670
BsnI GGCC 2 cut(s) 99, 890
Bso31I GGTCTC 1 cut(s) 38
Bsp143I GATC 6 cut(s) 80, 328, 409, 585, 856, 898
BspACI CCGC 2 cut(s) 515, 671
BspANI GGCC 2 cut(s) 99, 890
BspCNI CTCAG 2 cut(s) 338, 688
BspLI GGNNCC 2 cut(s) 128, 587
BspMAI CTGCAG 1 cut(s) 729
BspOI GCTAGC 1 cut(s) 485
BspPI GGATC 2 cut(s) 580, 593
BspQI GCTCTTC 1 cut(s) 51
BspTNI GGTCTC 1 cut(s) 38
BsrDI GCAATG 1 cut(s) 453
BsrFI RCCGGY 2 cut(s) 843, 886
BsrI ACTGG 1 cut(s) 891
BssAI RCCGGY 2 cut(s) 843, 886
BssECI CCNNGG 2 cut(s) 474, 688
BssMI GATC 6 cut(s) 80, 328, 409, 585, 856, 898
Bst2UI CCWGG 2 cut(s) 30, 690
Bst4CI ACNGT 3 cut(s) 89, 871, 877
Bst6I CTCTTC 6 cut(s) 51, 71, 176, 308, 338, 389
BstC8I GCNNGC 3 cut(s) 311, 483, 845
BstDEI CTNAG 4 cut(s) 13, 325, 696, 807
BstF5I GGATG 2 cut(s) 557, 744
BstH2I RGCGCY 1 cut(s) 482
BstHHI GCGC 1 cut(s) 481
BstKTI GATC 6 cut(s) 83, 331, 412, 588, 859, 901
BstMAI GTCTC 2 cut(s) 38, 469
BstMBI GATC 6 cut(s) 80, 328, 409, 585, 856, 898
BstMWI GCNNNNNNNGC 2 cut(s) 113, 296
BstNI CCWGG 2 cut(s) 30, 690
BstNSI RCATGY 3 cut(s) 260, 625, 768
BstSCI CCNGG 2 cut(s) 28, 688
BstSFI CTRYAG 2 cut(s) 633, 725
BstV2I GAAGAC 1 cut(s) 361
BstX2I RGATCY 3 cut(s) 80, 328, 585
BstYI RGATCY 3 cut(s) 80, 328, 585
BsuRI GGCC 2 cut(s) 99, 890
BtsCI GGATG 2 cut(s) 557, 744
BtsIMutI CAGTG 1 cut(s) 405
Cac8I GCNNGC 3 cut(s) 311, 483, 845
CfoI GCGC 1 cut(s) 481
Cfr10I RCCGGY 2 cut(s) 843, 886
Cfr13I GGNCC 1 cut(s) 98
CseI GACGC 1 cut(s) 824
Csp6I GTAC 1 cut(s) 829
CviAII CATG 6 cut(s) 257, 265, 568, 577, 622, 765
CviJI RGCY 8 cut(s) 99, 107, 157, 313, 485, 518, 677, 890
CviKI_1 RGCY 8 cut(s) 99, 107, 157, 313, 485, 518, 677, 890
CviQI GTAC 1 cut(s) 829
DdeI CTNAG 4 cut(s) 13, 325, 696, 807
DpnI GATC 6 cut(s) 82, 330, 411, 587, 858, 900
DpnII GATC 6 cut(s) 80, 328, 409, 585, 856, 898
DraIII CACNNNGTG 1 cut(s) 25
EaeI YGGCCR 1 cut(s) 888
Eam1104I CTCTTC 6 cut(s) 51, 71, 176, 308, 338, 389
EarI CTCTTC 6 cut(s) 51, 71, 176, 308, 338, 389
Eco31I GGTCTC 1 cut(s) 38
Eco32I GATATC 1 cut(s) 534
Eco57I CTGAAG 1 cut(s) 123
EcoO109I RGGNCCY 1 cut(s) 98
EcoRII CCWGG 2 cut(s) 28, 688
EcoRV GATATC 1 cut(s) 534
Esp3I CGTCTC 1 cut(s) 469
FaeI CATG 6 cut(s) 260, 268, 571, 580, 625, 768
FaqI GGGAC 1 cut(s) 670
FatI CATG 6 cut(s) 256, 264, 567, 576, 621, 764
FbaI TGATCA 1 cut(s) 409
Fnu4HI GCNGC 1 cut(s) 516
FokI GGATG 2 cut(s) 564, 751
Fsp4HI GCNGC 1 cut(s) 516
FspBI CTAG 4 cut(s) 206, 251, 482, 603
GlaI GCGC 1 cut(s) 480
GluI GCNGC 1 cut(s) 516
HaeII RGCGCY 1 cut(s) 482
HaeIII GGCC 2 cut(s) 99, 890
HapII CCGG 4 cut(s) 339, 789, 844, 887
HgaI GACGC 1 cut(s) 824
HhaI GCGC 1 cut(s) 481
Hin1II CATG 6 cut(s) 260, 268, 571, 580, 625, 768
Hin6I GCGC 1 cut(s) 479
HinP1I GCGC 1 cut(s) 479
HincII GTYRAC 1 cut(s) 627
HindII GTYRAC 1 cut(s) 627
HindIII AAGCTT 1 cut(s) 105
HinfI GANTC 3 cut(s) 166, 683, 721
HpaII CCGG 4 cut(s) 339, 789, 844, 887
HphI GGTGA 1 cut(s) 193
Hpy166II GTNNAC 1 cut(s) 627
Hpy188I TCNGA 3 cut(s) 328, 350, 898
Hpy188III TCNNGA 6 cut(s) 152, 163, 377, 422, 529, 562
Hpy8I GTNNAC 1 cut(s) 627
HpyAV CCTTC 7 cut(s) 190, 398, 419, 452, 478, 517, 642
HpyCH4III ACNGT 3 cut(s) 89, 871, 877
HpyCH4IV ACGT 1 cut(s) 8
HpyCH4V TGCA 3 cut(s) 116, 727, 866
HpyF10VI GCNNNNNNNGC 2 cut(s) 113, 296
HpyF3I CTNAG 4 cut(s) 13, 325, 696, 807
HpySE526I ACGT 1 cut(s) 8
Hsp92II CATG 6 cut(s) 260, 268, 571, 580, 625, 768
HspAI GCGC 1 cut(s) 479
KroI GCCGGC 1 cut(s) 843
KroNI GCCGGC 1 cut(s) 845
Ksp22I TGATCA 1 cut(s) 409
Kzo9I GATC 6 cut(s) 80, 328, 409, 585, 856, 898
LguI GCTCTTC 1 cut(s) 51
LweI GCATC 2 cut(s) 70, 542
MaeI CTAG 4 cut(s) 206, 251, 482, 603
MaeII ACGT 1 cut(s) 8
MaeIII GTNAC 3 cut(s) 428, 617, 871
MalI GATC 6 cut(s) 82, 330, 411, 587, 858, 900
MboI GATC 6 cut(s) 80, 328, 409, 585, 856, 898
MboII GAAGA 9 cut(s) 38, 58, 163, 166, 295, 325, 361, 376, 383
MflI RGATCY 3 cut(s) 80, 328, 585
MluCI AATT 2 cut(s) 119, 217
MlyI GAGTC 1 cut(s) 692
MmeI TCCRAC 3 cut(s) 472, 716, 804
MroNI GCCGGC 1 cut(s) 843
MseI TTAA 1 cut(s) 93
MslI CAYNNNNRTG 1 cut(s) 666
MspI CCGG 4 cut(s) 339, 789, 844, 887
MspR9I CCNGG 2 cut(s) 30, 690
MvaI CCWGG 2 cut(s) 30, 690
MwoI GCNNNNNNNGC 2 cut(s) 113, 296
NaeI GCCGGC 1 cut(s) 845
NdeII GATC 6 cut(s) 80, 328, 409, 585, 856, 898
NgoMIV GCCGGC 1 cut(s) 843
NheI GCTAGC 1 cut(s) 481
NlaIII CATG 6 cut(s) 260, 268, 571, 580, 625, 768
NlaIV GGNNCC 2 cut(s) 128, 587
NmeAIII GCCGAG 1 cut(s) 455
NmuCI GTSAC 1 cut(s) 428
NspI RCATGY 3 cut(s) 260, 625, 768
PciI ACATGT 2 cut(s) 621, 764
PciSI GCTCTTC 1 cut(s) 51
PdiI GCCGGC 1 cut(s) 845
PfeI GAWTC 2 cut(s) 166, 721
PkrI GCNGC 1 cut(s) 517
PleI GAGTC 1 cut(s) 691
PpsI GAGTC 1 cut(s) 691
PscI ACATGT 2 cut(s) 621, 764
Psp1406I AACGTT 1 cut(s) 8
Psp6I CCWGG 2 cut(s) 28, 688
PspGI CCWGG 2 cut(s) 28, 688
PspN4I GGNNCC 2 cut(s) 128, 587
PspPI GGNCC 1 cut(s) 98
PstI CTGCAG 1 cut(s) 729
PsuI RGATCY 3 cut(s) 80, 328, 585
RsaI GTAC 1 cut(s) 830
RsaNI GTAC 1 cut(s) 829
RseI CAYNNNNRTG 1 cut(s) 666
SapI GCTCTTC 1 cut(s) 51
SaqAI TTAA 1 cut(s) 93
SatI GCNGC 1 cut(s) 516
Sau3AI GATC 6 cut(s) 80, 328, 409, 585, 856, 898
Sau96I GGNCC 1 cut(s) 98
SchI GAGTC 1 cut(s) 692
ScrFI CCNGG 2 cut(s) 30, 690
SfaNI GCATC 2 cut(s) 70, 542
SfcI CTRYAG 2 cut(s) 633, 725
SmiMI CAYNNNNRTG 1 cut(s) 666
SmlI CTYRAG 3 cut(s) 152, 375, 779
SmoI CTYRAG 3 cut(s) 152, 375, 779
Sse9I AATT 2 cut(s) 119, 217
SsiI CCGC 2 cut(s) 515, 671
SspMI CTAG 4 cut(s) 206, 251, 482, 603
StyD4I CCNGG 2 cut(s) 28, 688
TaaI ACNGT 3 cut(s) 89, 871, 877
TaiI ACGT 1 cut(s) 11
TasI AATT 2 cut(s) 119, 217
TauI GCSGC 1 cut(s) 518
TfiI GAWTC 2 cut(s) 166, 721
Tru1I TTAA 1 cut(s) 93
Tru9I TTAA 1 cut(s) 93
TscAI CASTG 1 cut(s) 412
TseFI GTSAC 1 cut(s) 428
Tsp45I GTSAC 1 cut(s) 428
TspDTI ATGAA 3 cut(s) 383, 389, 565
TspRI CASTG 1 cut(s) 412
XapI RAATTY 1 cut(s) 119
XceI RCATGY 3 cut(s) 260, 625, 768
XspI CTAG 4 cut(s) 206, 251, 482, 603
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.