Family of unknown function (DUF716)
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc65I | GGTACC | 1 cut(s) | 126 |
| AccB1I | GGYRCC | 1 cut(s) | 126 |
| AfaI | GTAC | 2 cut(s) | 27, 128 |
| AgsI | TTSAA | 1 cut(s) | 136 |
| AjnI | CCWGG | 1 cut(s) | 28 |
| AoxI | GGCC | 1 cut(s) | 97 |
| Asp718I | GGTACC | 1 cut(s) | 126 |
| AspS9I | GGNCC | 1 cut(s) | 98 |
| AsuHPI | GGTGA | 1 cut(s) | 34 |
| BaeGI | GKGCMC | 2 cut(s) | 8, 21 |
| BanI | GGYRCC | 1 cut(s) | 126 |
| BciT130I | CCWGG | 1 cut(s) | 30 |
| BfaI | CTAG | 1 cut(s) | 102 |
| BglII | AGATCT | 1 cut(s) | 80 |
| Bme1390I | CCNGG | 1 cut(s) | 30 |
| BmgT120I | GGNCC | 1 cut(s) | 98 |
| BmiI | GGNNCC | 1 cut(s) | 128 |
| BmrFI | CCNGG | 1 cut(s) | 30 |
| BseBI | CCWGG | 1 cut(s) | 30 |
| BseSI | GKGCMC | 2 cut(s) | 8, 21 |
| BshFI | GGCC | 1 cut(s) | 99 |
| BshNI | GGYRCC | 1 cut(s) | 126 |
| BsnI | GGCC | 1 cut(s) | 99 |
| Bsp1286I | GDGCHC | 2 cut(s) | 8, 21 |
| Bsp143I | GATC | 1 cut(s) | 80 |
| BspANI | GGCC | 1 cut(s) | 99 |
| BspLI | GGNNCC | 1 cut(s) | 128 |
| BspT107I | GGYRCC | 1 cut(s) | 126 |
| BssMI | GATC | 1 cut(s) | 80 |
| Bst2UI | CCWGG | 1 cut(s) | 30 |
| BstKTI | GATC | 1 cut(s) | 83 |
| BstMBI | GATC | 1 cut(s) | 80 |
| BstNI | CCWGG | 1 cut(s) | 30 |
| BstSCI | CCNGG | 1 cut(s) | 28 |
| BstSLI | GKGCMC | 2 cut(s) | 8, 21 |
| BstX2I | RGATCY | 1 cut(s) | 80 |
| BstYI | RGATCY | 1 cut(s) | 80 |
| BsuRI | GGCC | 1 cut(s) | 99 |
| BtsI | GCAGTG | 1 cut(s) | 63 |
| BtsIMutI | CAGTG | 1 cut(s) | 63 |
| Cfr13I | GGNCC | 1 cut(s) | 98 |
| Csp6I | GTAC | 2 cut(s) | 26, 127 |
| CviJI | RGCY | 2 cut(s) | 33, 99 |
| CviKI_1 | RGCY | 2 cut(s) | 33, 99 |
| CviQI | GTAC | 2 cut(s) | 26, 127 |
| DpnI | GATC | 1 cut(s) | 82 |
| DpnII | GATC | 1 cut(s) | 80 |
| EcoO109I | RGGNCCY | 1 cut(s) | 98 |
| EcoRII | CCWGG | 1 cut(s) | 28 |
| FaiI | YATR | 4 cut(s) | 23, 58, 179, 181 |
| FauNDI | CATATG | 1 cut(s) | 179 |
| FspBI | CTAG | 1 cut(s) | 102 |
| HaeIII | GGCC | 1 cut(s) | 99 |
| HphI | GGTGA | 1 cut(s) | 34 |
| Hpy188I | TCNGA | 1 cut(s) | 80 |
| HpyAV | CCTTC | 1 cut(s) | 56 |
| HpyCH4IV | ACGT | 1 cut(s) | 8 |
| HpySE526I | ACGT | 1 cut(s) | 8 |
| KpnI | GGTACC | 1 cut(s) | 130 |
| Kzo9I | GATC | 1 cut(s) | 80 |
| LpnPI | CCDG | 2 cut(s) | 15, 42 |
| MaeI | CTAG | 1 cut(s) | 102 |
| MaeII | ACGT | 1 cut(s) | 8 |
| MaeIII | GTNAC | 1 cut(s) | 104 |
| MalI | GATC | 1 cut(s) | 82 |
| MboI | GATC | 1 cut(s) | 80 |
| MflI | RGATCY | 1 cut(s) | 80 |
| MhlI | GDGCHC | 2 cut(s) | 8, 21 |
| MluCI | AATT | 1 cut(s) | 153 |
| MnlI | CCTC | 1 cut(s) | 50 |
| MseI | TTAA | 1 cut(s) | 156 |
| MspR9I | CCNGG | 1 cut(s) | 30 |
| MvaI | CCWGG | 1 cut(s) | 30 |
| NdeI | CATATG | 1 cut(s) | 179 |
| NdeII | GATC | 1 cut(s) | 80 |
| NlaIV | GGNNCC | 1 cut(s) | 128 |
| Psp6I | CCWGG | 1 cut(s) | 28 |
| PspGI | CCWGG | 1 cut(s) | 28 |
| PspN4I | GGNNCC | 1 cut(s) | 128 |
| PspPI | GGNCC | 1 cut(s) | 98 |
| PsuI | RGATCY | 1 cut(s) | 80 |
| RsaI | GTAC | 2 cut(s) | 27, 128 |
| RsaNI | GTAC | 2 cut(s) | 26, 127 |
| SaqAI | TTAA | 1 cut(s) | 156 |
| Sau3AI | GATC | 1 cut(s) | 80 |
| Sau96I | GGNCC | 1 cut(s) | 98 |
| ScrFI | CCNGG | 1 cut(s) | 30 |
| SduI | GDGCHC | 2 cut(s) | 8, 21 |
| SetI | ASST | 3 cut(s) | 11, 31, 122 |
| SgeI | CNNG | 6 cut(s) | 19, 41, 42, 62, 114, 175 |
| Sse9I | AATT | 1 cut(s) | 153 |
| SspMI | CTAG | 1 cut(s) | 102 |
| StyD4I | CCNGG | 1 cut(s) | 28 |
| TaiI | ACGT | 1 cut(s) | 11 |
| TasI | AATT | 1 cut(s) | 153 |
| Tru1I | TTAA | 1 cut(s) | 156 |
| Tru9I | TTAA | 1 cut(s) | 156 |
| TscAI | CASTG | 1 cut(s) | 70 |
| TspDTI | ATGAA | 1 cut(s) | 166 |
| TspRI | CASTG | 1 cut(s) | 70 |
| XspI | CTAG | 1 cut(s) | 102 |