Belongs to the helicase family. Dicer subfamily
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB7I | CCANNNNNTGG | 1 cut(s) | 139 |
| AciI | CCGC | 4 cut(s) | 60, 63, 66, 69 |
| AcyI | GRCGYC | 1 cut(s) | 57 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 11, 43, 102, 139 |
| AjnI | CCWGG | 2 cut(s) | 23, 37 |
| Alw21I | GWGCWC | 1 cut(s) | 132 |
| AspS9I | GGNCC | 1 cut(s) | 21 |
| AsuHPI | GGTGA | 1 cut(s) | 62 |
| AvaII | GGWCC | 1 cut(s) | 21 |
| Bbv12I | GWGCWC | 1 cut(s) | 132 |
| BccI | CCATC | 1 cut(s) | 80 |
| BciT130I | CCWGG | 2 cut(s) | 25, 39 |
| BisI | GCNGC | 4 cut(s) | 60, 63, 66, 69 |
| BlsI | GCNGC | 4 cut(s) | 61, 64, 67, 70 |
| Bme1390I | CCNGG | 2 cut(s) | 25, 39 |
| Bme18I | GGWCC | 1 cut(s) | 21 |
| BmgT120I | GGNCC | 1 cut(s) | 21 |
| BmiI | GGNNCC | 1 cut(s) | 22 |
| BmrFI | CCNGG | 2 cut(s) | 25, 39 |
| Bpu10I | CCTNAGC | 1 cut(s) | 16 |
| BsaHI | GRCGYC | 1 cut(s) | 57 |
| BsaJI | CCNNGG | 1 cut(s) | 37 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 11, 43, 102, 139 |
| Bse1I | ACTGG | 2 cut(s) | 217, 256 |
| Bse3DI | GCAATG | 1 cut(s) | 150 |
| BseBI | CCWGG | 2 cut(s) | 25, 39 |
| BseDI | CCNNGG | 1 cut(s) | 37 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 11, 43, 102, 139 |
| BseMI | GCAATG | 1 cut(s) | 150 |
| BseMII | CTCAG | 1 cut(s) | 287 |
| BseNI | ACTGG | 2 cut(s) | 217, 256 |
| BsiHKAI | GWGCWC | 1 cut(s) | 132 |
| BslFI | GGGAC | 1 cut(s) | 34 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 11, 43, 102, 139 |
| BsmFI | GGGAC | 1 cut(s) | 34 |
| Bsp1286I | GDGCHC | 1 cut(s) | 132 |
| BspACI | CCGC | 4 cut(s) | 60, 63, 66, 69 |
| BspCNI | CTCAG | 1 cut(s) | 286 |
| BspLI | GGNNCC | 1 cut(s) | 22 |
| BsrDI | GCAATG | 1 cut(s) | 150 |
| BsrI | ACTGG | 2 cut(s) | 217, 256 |
| BssECI | CCNNGG | 1 cut(s) | 37 |
| BssNI | GRCGYC | 1 cut(s) | 57 |
| Bst2UI | CCWGG | 2 cut(s) | 25, 39 |
| Bst4CI | ACNGT | 2 cut(s) | 171, 237 |
| BstACI | GRCGYC | 1 cut(s) | 57 |
| BstC8I | GCNNGC | 1 cut(s) | 104 |
| BstDEI | CTNAG | 2 cut(s) | 16, 273 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 65, 68 |
| BstNI | CCWGG | 2 cut(s) | 25, 39 |
| BstSCI | CCNGG | 2 cut(s) | 23, 37 |
| Cac8I | GCNNGC | 1 cut(s) | 104 |
| Cfr13I | GGNCC | 1 cut(s) | 21 |
| CseI | GACGC | 1 cut(s) | 65 |
| CviJI | RGCY | 4 cut(s) | 15, 36, 42, 182 |
| CviKI_1 | RGCY | 4 cut(s) | 15, 36, 42, 182 |
| DdeI | CTNAG | 2 cut(s) | 16, 273 |
| Eco32I | GATATC | 1 cut(s) | 112 |
| Eco47I | GGWCC | 1 cut(s) | 21 |
| EcoO109I | RGGNCCY | 1 cut(s) | 21 |
| EcoRII | CCWGG | 2 cut(s) | 23, 37 |
| EcoRV | GATATC | 1 cut(s) | 112 |
| FaiI | YATR | 7 cut(s) | 119, 140, 194, 233, 247, 251, 286 |
| FaqI | GGGAC | 1 cut(s) | 34 |
| Fnu4HI | GCNGC | 4 cut(s) | 60, 63, 66, 69 |
| Fsp4HI | GCNGC | 4 cut(s) | 60, 63, 66, 69 |
| GluI | GCNGC | 4 cut(s) | 60, 63, 66, 69 |
| HgaI | GACGC | 1 cut(s) | 65 |
| Hin1I | GRCGYC | 1 cut(s) | 57 |
| HinfI | GANTC | 1 cut(s) | 89 |
| HphI | GGTGA | 1 cut(s) | 62 |
| Hpy188I | TCNGA | 2 cut(s) | 276, 296 |
| Hpy188III | TCNNGA | 1 cut(s) | 206 |
| Hpy99I | CGWCG | 1 cut(s) | 59 |
| HpyAV | CCTTC | 3 cut(s) | 15, 144, 172 |
| HpyCH4III | ACNGT | 2 cut(s) | 171, 237 |
| HpyCH4IV | ACGT | 1 cut(s) | 282 |
| HpyCH4V | TGCA | 1 cut(s) | 102 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 65, 68 |
| HpyF3I | CTNAG | 2 cut(s) | 16, 273 |
| HpySE526I | ACGT | 1 cut(s) | 282 |
| Hsp92I | GRCGYC | 1 cut(s) | 57 |
| LmnI | GCTCC | 1 cut(s) | 135 |
| MaeII | ACGT | 1 cut(s) | 282 |
| MaeIII | GTNAC | 1 cut(s) | 50 |
| MboII | GAAGA | 2 cut(s) | 84, 289 |
| MfeI | CAATTG | 2 cut(s) | 97, 161 |
| MhlI | GDGCHC | 1 cut(s) | 132 |
| MluCI | AATT | 3 cut(s) | 97, 161, 195 |
| MnlI | CCTC | 2 cut(s) | 147, 282 |
| MslI | CAYNNNNRTG | 1 cut(s) | 197 |
| MspR9I | CCNGG | 2 cut(s) | 25, 39 |
| MunI | CAATTG | 2 cut(s) | 97, 161 |
| MvaI | CCWGG | 2 cut(s) | 25, 39 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 65, 68 |
| NlaIV | GGNNCC | 1 cut(s) | 22 |
| NmuCI | GTSAC | 1 cut(s) | 50 |
| PfeI | GAWTC | 1 cut(s) | 89 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 139 |
| PkrI | GCNGC | 4 cut(s) | 61, 64, 67, 70 |
| PpuMI | RGGWCCY | 1 cut(s) | 21 |
| Psp5II | RGGWCCY | 1 cut(s) | 21 |
| Psp6I | CCWGG | 2 cut(s) | 23, 37 |
| PspGI | CCWGG | 2 cut(s) | 23, 37 |
| PspN4I | GGNNCC | 1 cut(s) | 22 |
| PspPI | GGNCC | 1 cut(s) | 21 |
| PspPPI | RGGWCCY | 1 cut(s) | 21 |
| RseI | CAYNNNNRTG | 1 cut(s) | 197 |
| SatI | GCNGC | 4 cut(s) | 60, 63, 66, 69 |
| Sau96I | GGNCC | 1 cut(s) | 21 |
| ScrFI | CCNGG | 2 cut(s) | 25, 39 |
| SduI | GDGCHC | 1 cut(s) | 132 |
| SetI | ASST | 4 cut(s) | 26, 158, 274, 285 |
| SinI | GGWCC | 1 cut(s) | 21 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 197 |
| Sse9I | AATT | 3 cut(s) | 97, 161, 195 |
| SsiI | CCGC | 4 cut(s) | 60, 63, 66, 69 |
| StyD4I | CCNGG | 2 cut(s) | 23, 37 |
| TaaI | ACNGT | 2 cut(s) | 171, 237 |
| TaiI | ACGT | 1 cut(s) | 285 |
| TasI | AATT | 3 cut(s) | 97, 161, 195 |
| TauI | GCSGC | 4 cut(s) | 62, 65, 68, 71 |
| TfiI | GAWTC | 1 cut(s) | 89 |
| TseFI | GTSAC | 1 cut(s) | 50 |
| Tsp45I | GTSAC | 1 cut(s) | 50 |
| TspDTI | ATGAA | 1 cut(s) | 266 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 139 |
| VpaK11BI | GGWCC | 1 cut(s) | 21 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 40 |