Prupe.8G112800_v2.0.a1

Family of unknown function (DUF716)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp08
OG0001745
Physical Location & Seq
Forward (+)
14144958 .. 14146536
1579 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.8G112800.1

Sequence Viewer

Length: 888 bp
ATGGGCACGTTTGTTGGACACATAGTTCCCGGGTTTGCACTCACCCTCCTTGGTTTATGGCACACCATCAACACTATCAGATCTTATTTTCTCAGGGGCCCTAGTAACTTTAGAGTAAGGTTTTGGCACCCATTCAATTCCCCTCTTTCCAAACTCAAACACTTGGAGCTCATTTTCATCTTATCTTTCTCTGTATTGGCAATTTTCATGCAAGTTTTAGACTTCCCTTTTCTTCAACTCTCTTTCAAGCTCCACAACTTGGAGCATGCAACTATGTTCCTCCACCTAGTCGTATTTGCAGGATTTACGCTTTGTGCTGAGTTGATTCATCCGTTTCAGACCCTATCTGGGGTCGTTGGAATGCTAGTAGCATCTGTTTTCTGTCAAGAGCTTTTCCTACTTCATTTCCACTCTGCTGACCATGTTGGTCTTGAAGGGCATTACCATTGGCTGCTGCAGCTTATAGTGTTTGTTTCTGTCATGGCAGCTTTGGCAGCAACTTGTTGCCAAACCAGTCTTCCTGCAGCACTTGTTCTTTCAATTTCAGTGGTATTTCAAGGTTGCTGGTTTATGAACATGGGGTTTTTGCTCTGGGTTCCTAGATTTGTTCCAAAGGGTTGTGTTGTCCATTATGTAGAGGGCACCAATGATACCATGCTTGGAGCCGTAACCTGTCAGTCAAGTGAGGCTGATTTTAGGGCAAGAGCATTGGCCAACTTGCAATTTAGTTGGATACTTTCTGGAATTTTGATATTCACAGGTTGTGCATGCCTGAAATTTGCTGTCAAATGCATTCCAAGGGACCTGCCAATAGAGTATGAGCAACTCCATAGCAGAGGAGCAGATGTCCCTATAGTCATAAATGATTTCAAGGAAGCTCATCCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF716

Protein Analysis

296

Amino Acids

33.1

Weight (kDa)

6.95

Isoelectric Point (pI)

31.67

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 813
AccB1I GGYRCC 2 cut(s) 126, 641
AccB7I CCANNNNNTGG 1 cut(s) 259
AcoI YGGCCR 1 cut(s) 711
AcsI RAATTY 2 cut(s) 744, 776
AfiI CCNNNNNNNGG 3 cut(s) 259, 348, 349
AgsI TTSAA 7 cut(s) 136, 236, 247, 434, 540, 557, 871
AjuI GAANNNNNNNTTGG 2 cut(s) 501, 533
AloI GAACNNNNNNTCC 2 cut(s) 9, 41
AluBI AGCT 6 cut(s) 169, 250, 391, 460, 488, 878
AluI AGCT 6 cut(s) 169, 250, 391, 460, 488, 878
Alw21I GWGCWC 1 cut(s) 171
Ama87I CYCGRG 1 cut(s) 29
AoxI GGCC 2 cut(s) 97, 711
ApaI GGGCCC 1 cut(s) 101
ApeKI GCWGC 6 cut(s) 451, 454, 457, 485, 494, 524
ApoI RAATTY 2 cut(s) 744, 776
AspS9I GGNCC 3 cut(s) 97, 98, 802
AsuC2I CCSGG 2 cut(s) 30, 31
AsuHPI GGTGA 1 cut(s) 34
AvaI CYCGRG 1 cut(s) 29
AvaII GGWCC 1 cut(s) 802
BaeGI GKGCMC 3 cut(s) 8, 101, 644
BalI TGGCCA 1 cut(s) 713
BanI GGYRCC 2 cut(s) 126, 641
BanII GRGCYC 2 cut(s) 101, 171
BbsI GAAGAC 1 cut(s) 509
Bbv12I GWGCWC 1 cut(s) 171
BbvI GCAGC 6 cut(s) 438, 441, 469, 497, 506, 536
BccI CCATC 1 cut(s) 74
BceAI ACGGC 1 cut(s) 650
BciVI GTATCC 1 cut(s) 726
BcnI CCSGG 2 cut(s) 30, 31
BfaI CTAG 4 cut(s) 102, 287, 365, 600
BfmI CTRYAG 3 cut(s) 455, 522, 852
BfuAI ACCTGC 1 cut(s) 813
BfuI GTATCC 1 cut(s) 726
BglII AGATCT 1 cut(s) 80
BisI GCNGC 6 cut(s) 452, 455, 458, 486, 495, 525
BlsI GCNGC 6 cut(s) 453, 456, 459, 487, 496, 526
Bme1390I CCNGG 2 cut(s) 30, 31
Bme18I GGWCC 1 cut(s) 802
BmeT110I CYCGRG 1 cut(s) 29
BmgT120I GGNCC 3 cut(s) 97, 98, 802
BmiI GGNNCC 7 cut(s) 98, 99, 128, 597, 643, 664, 803
BmrFI CCNGG 2 cut(s) 30, 31
BmsI GCATC 1 cut(s) 380
BpiI GAAGAC 1 cut(s) 509
BpuMI CCSGG 2 cut(s) 30, 31
BsaJI CCNNGG 3 cut(s) 29, 49, 797
BsaXI ACNNNNNCTCC 4 cut(s) 30, 60, 831, 861
Bsc4I CCNNNNNNNGG 3 cut(s) 259, 348, 349
Bse1I ACTGG 1 cut(s) 513
BseDI CCNNGG 3 cut(s) 29, 49, 797
BseGI GGATG 2 cut(s) 328, 880
BseLI CCNNNNNNNGG 3 cut(s) 259, 348, 349
BseMII CTCAG 2 cut(s) 106, 309
BseNI ACTGG 1 cut(s) 513
BseRI GAGGAG 1 cut(s) 852
BseSI GKGCMC 3 cut(s) 8, 101, 644
BseXI GCAGC 6 cut(s) 438, 441, 469, 497, 506, 536
BshFI GGCC 2 cut(s) 99, 713
BshNI GGYRCC 2 cut(s) 126, 641
BsiHKAI GWGCWC 1 cut(s) 171
BsiHKCI CYCGRG 1 cut(s) 29
BsiSI CCGG 1 cut(s) 30
BslFI GGGAC 2 cut(s) 815, 833
BslI CCNNNNNNNGG 3 cut(s) 259, 348, 349
BsmFI GGGAC 2 cut(s) 815, 833
BsmI GAATGC 2 cut(s) 366, 792
BsnI GGCC 2 cut(s) 99, 713
BsoBI CYCGRG 1 cut(s) 29
Bsp120I GGGCCC 1 cut(s) 97
Bsp1286I GDGCHC 4 cut(s) 8, 101, 171, 644
Bsp143I GATC 1 cut(s) 80
BspANI GGCC 2 cut(s) 99, 713
BspCNI CTCAG 2 cut(s) 105, 310
BspLI GGNNCC 7 cut(s) 98, 99, 128, 597, 643, 664, 803
BspMAI CTGCAG 2 cut(s) 459, 526
BspMI ACCTGC 1 cut(s) 813
BspT107I GGYRCC 2 cut(s) 126, 641
BsrI ACTGG 1 cut(s) 513
BssECI CCNNGG 3 cut(s) 29, 49, 797
BssMI GATC 1 cut(s) 80
BssT1I CCWWGG 2 cut(s) 49, 797
BstC8I GCNNGC 2 cut(s) 267, 769
BstDEI CTNAG 2 cut(s) 92, 318
BstF5I GGATG 2 cut(s) 328, 880
BstKTI GATC 1 cut(s) 83
BstMBI GATC 1 cut(s) 80
BstMWI GCNNNNNNNGC 3 cut(s) 457, 491, 494
BstNSI RCATGY 2 cut(s) 269, 771
BstSCI CCNGG 2 cut(s) 28, 29
BstSFI CTRYAG 3 cut(s) 455, 522, 852
BstSLI GKGCMC 3 cut(s) 8, 101, 644
BstV1I GCAGC 6 cut(s) 438, 441, 469, 497, 506, 536
BstV2I GAAGAC 1 cut(s) 509
BstX2I RGATCY 1 cut(s) 80
BstYI RGATCY 1 cut(s) 80
BsuI GTATCC 1 cut(s) 726
BsuRI GGCC 2 cut(s) 99, 713
BtsCI GGATG 2 cut(s) 328, 880
BtsIMutI CAGTG 1 cut(s) 552
BveI ACCTGC 1 cut(s) 813
Cac8I GCNNGC 2 cut(s) 267, 769
Cfr13I GGNCC 3 cut(s) 97, 98, 802
Cfr9I CCCGGG 1 cut(s) 29
CspCI CAANNNNNGTGG 2 cut(s) 528, 563
CviAII CATG 7 cut(s) 208, 266, 422, 481, 577, 655, 768
DdeI CTNAG 2 cut(s) 92, 318
DpnI GATC 1 cut(s) 82
DpnII GATC 1 cut(s) 80
EaeI YGGCCR 1 cut(s) 711
Ecl136II GAGCTC 1 cut(s) 169
Eco130I CCWWGG 2 cut(s) 49, 797
Eco24I GRGCYC 2 cut(s) 101, 171
Eco47I GGWCC 1 cut(s) 802
Eco53kI GAGCTC 1 cut(s) 169
Eco88I CYCGRG 1 cut(s) 29
EcoICRI GAGCTC 1 cut(s) 169
EcoO109I RGGNCCY 3 cut(s) 97, 98, 802
EcoT14I CCWWGG 2 cut(s) 49, 797
EcoT22I ATGCAT 1 cut(s) 794
EcoT38I GRGCYC 2 cut(s) 101, 171
ErhI CCWWGG 2 cut(s) 49, 797
FaeI CATG 7 cut(s) 211, 269, 425, 484, 580, 658, 771
FaqI GGGAC 2 cut(s) 815, 833
FatI CATG 7 cut(s) 207, 265, 421, 480, 576, 654, 767
Fnu4HI GCNGC 6 cut(s) 452, 455, 458, 486, 495, 525
FokI GGATG 2 cut(s) 315, 867
FriOI GRGCYC 2 cut(s) 101, 171
Fsp4HI GCNGC 6 cut(s) 452, 455, 458, 486, 495, 525
FspBI CTAG 4 cut(s) 102, 287, 365, 600
GluI GCNGC 6 cut(s) 452, 455, 458, 486, 495, 525
HaeIII GGCC 2 cut(s) 99, 713
HapII CCGG 1 cut(s) 30
Hin1II CATG 7 cut(s) 211, 269, 425, 484, 580, 658, 771
HinfI GANTC 1 cut(s) 325
HpaII CCGG 1 cut(s) 30
HphI GGTGA 1 cut(s) 34
Hpy188I TCNGA 2 cut(s) 80, 339
Hpy188III TCNNGA 3 cut(s) 386, 431, 741
HpyAV CCTTC 1 cut(s) 428
HpyCH4IV ACGT 1 cut(s) 8
HpyCH4V TGCA 9 cut(s) 38, 211, 269, 299, 457, 524, 721, 767, 792
HpyF10VI GCNNNNNNNGC 3 cut(s) 457, 491, 494
HpyF3I CTNAG 2 cut(s) 92, 318
HpySE526I ACGT 1 cut(s) 8
Hsp92II CATG 7 cut(s) 211, 269, 425, 484, 580, 658, 771
Kzo9I GATC 1 cut(s) 80
LmnI GCTCC 5 cut(s) 166, 255, 262, 662, 839
Lsp1109I GCAGC 6 cut(s) 438, 441, 469, 497, 506, 536
LweI GCATC 1 cut(s) 380
MaeI CTAG 4 cut(s) 102, 287, 365, 600
MaeII ACGT 1 cut(s) 8
MaeIII GTNAC 2 cut(s) 104, 667
MalI GATC 1 cut(s) 82
MboI GATC 1 cut(s) 80
MboII GAAGA 2 cut(s) 224, 509
MflI RGATCY 1 cut(s) 80
MhlI GDGCHC 4 cut(s) 8, 101, 171, 644
MlsI TGGCCA 1 cut(s) 713
MluCI AATT 6 cut(s) 136, 201, 540, 722, 744, 776
MluNI TGGCCA 1 cut(s) 713
MmeI TCCRAC 2 cut(s) 337, 710
MnlI CCTC 6 cut(s) 56, 153, 290, 631, 679, 830
Mox20I TGGCCA 1 cut(s) 713
Mph1103I ATGCAT 1 cut(s) 794
MscI TGGCCA 1 cut(s) 713
Msp20I TGGCCA 1 cut(s) 713
MspI CCGG 1 cut(s) 30
MspR9I CCNGG 2 cut(s) 30, 31
Mva1269I GAATGC 2 cut(s) 366, 792
MwoI GCNNNNNNNGC 3 cut(s) 457, 491, 494
NciI CCSGG 2 cut(s) 30, 31
NdeII GATC 1 cut(s) 80
NlaIII CATG 7 cut(s) 211, 269, 425, 484, 580, 658, 771
NlaIV GGNNCC 7 cut(s) 98, 99, 128, 597, 643, 664, 803
NsiI ATGCAT 1 cut(s) 794
NspI RCATGY 2 cut(s) 269, 771
PaeI GCATGC 2 cut(s) 269, 771
PctI GAATGC 2 cut(s) 366, 792
PfeI GAWTC 1 cut(s) 325
PflMI CCANNNNNTGG 1 cut(s) 259
PkrI GCNGC 6 cut(s) 453, 456, 459, 487, 496, 526
PpuMI RGGWCCY 1 cut(s) 802
Psp124BI GAGCTC 1 cut(s) 171
Psp5II RGGWCCY 1 cut(s) 802
PspN4I GGNNCC 7 cut(s) 98, 99, 128, 597, 643, 664, 803
PspOMI GGGCCC 1 cut(s) 97
PspPI GGNCC 3 cut(s) 97, 98, 802
PspPPI RGGWCCY 1 cut(s) 802
PstI CTGCAG 2 cut(s) 459, 526
PsuI RGATCY 1 cut(s) 80
SacI GAGCTC 1 cut(s) 171
SatI GCNGC 6 cut(s) 452, 455, 458, 486, 495, 525
Sau3AI GATC 1 cut(s) 80
Sau96I GGNCC 3 cut(s) 97, 98, 802
ScrFI CCNGG 2 cut(s) 30, 31
SduI GDGCHC 4 cut(s) 8, 101, 171, 644
SfaNI GCATC 1 cut(s) 380
SfcI CTRYAG 3 cut(s) 455, 522, 852
SinI GGWCC 1 cut(s) 802
SmaI CCCGGG 1 cut(s) 31
SphI GCATGC 2 cut(s) 269, 771
Sse9I AATT 6 cut(s) 136, 201, 540, 722, 744, 776
SspMI CTAG 4 cut(s) 102, 287, 365, 600
SstI GAGCTC 1 cut(s) 171
StyD4I CCNGG 2 cut(s) 28, 29
StyI CCWWGG 2 cut(s) 49, 797
TaiI ACGT 1 cut(s) 11
TasI AATT 6 cut(s) 136, 201, 540, 722, 744, 776
TfiI GAWTC 1 cut(s) 325
TscAI CASTG 1 cut(s) 552
TseI GCWGC 6 cut(s) 451, 454, 457, 485, 494, 524
TspDTI ATGAA 5 cut(s) 166, 196, 317, 392, 587
TspGWI ACGGA 1 cut(s) 321
TspMI CCCGGG 1 cut(s) 29
TspRI CASTG 1 cut(s) 552
Van91I CCANNNNNTGG 1 cut(s) 259
VpaK11BI GGWCC 1 cut(s) 802
XapI RAATTY 2 cut(s) 744, 776
XceI RCATGY 2 cut(s) 269, 771
XmaI CCCGGG 1 cut(s) 29
XspI CTAG 4 cut(s) 102, 287, 365, 600
Zsp2I ATGCAT 1 cut(s) 794
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.