Rw2G024940

Family of unknown function (DUF716)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0001745
Physical Location & Seq
Reverse (-)
37078378 .. 37078968
591 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G024940.1

Sequence Viewer

Length: 591 bp
ATGGGCACATTCATTGGGCACATAGTACCTGGCTTTGCCCTCACCCTTCTTGGTTTATGGCACACCGTCAACACTATCAGATCTTATTTTCTCAAAGGTCCTAGTAACTTTAGAGTAAGGTTTTGGTACCCATTGAACTGCCCTTTTCCCAAAATTAAACACCTTGAACTCATTTTCATCTTATCTTTCTCTGTATTTGCAATTATTATGCAAGTTCTAGAATTCCCGTTTCATAACTTCTCCTTCAAGCTCCATAATTTGGAGCATGCAACCATGTTCCTCCACCTTGCCATATTTGCAGGGTTTACACTTTCAGCTGAGTTTATTAATTCGTTTCAAATCATATCTGGGGTCGTTGGATTGCTTACGGCCTCTGTTTTCTGCCAAGAGCTTTTCCTTCTCCATTTCCACTCCACTGACCATGTTGGTCTTGAAGAGCATTACCATTGGTTATTGCAGCTCATAGTGTTTGCTATGCGGCAAGGTTGGTGCTTTACTATTTGCTTTGCGAATGATCCAATTTGCTCCATCATTCTTTTTGTGCAGAAAGATATTCATTGGAGGCTTGACAAAGAACACCACCTTGGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF716

Protein Analysis

196

Amino Acids

22.82

Weight (kDa)

6.94

Isoelectric Point (pI)

27.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF716 PF04819 117 - 175 3e-12 Family of unknown function (DUF716)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 126
AccB1I GGYRCC 1 cut(s) 126
AccB7I CCANNNNNTGG 1 cut(s) 259
AciI CCGC 1 cut(s) 478
AclWI GGATC 1 cut(s) 509
AcsI RAATTY 1 cut(s) 221
AfaI GTAC 2 cut(s) 27, 128
AfiI CCNNNNNNNGG 1 cut(s) 259
AgsI TTSAA 5 cut(s) 136, 167, 247, 338, 434
AjnI CCWGG 1 cut(s) 28
AjuI GAANNNNNNNTTGG 1 cut(s) 567
AluBI AGCT 4 cut(s) 250, 317, 391, 460
AluI AGCT 4 cut(s) 250, 317, 391, 460
AlwI GGATC 1 cut(s) 509
AoxI GGCC 1 cut(s) 369
ApeKI GCWGC 1 cut(s) 457
ApoI RAATTY 1 cut(s) 221
AseI ATTAAT 1 cut(s) 327
Asp718I GGTACC 1 cut(s) 126
AspS9I GGNCC 1 cut(s) 98
AsuHPI GGTGA 1 cut(s) 34
AvaII GGWCC 1 cut(s) 98
BaeGI GKGCMC 2 cut(s) 8, 21
BanI GGYRCC 1 cut(s) 126
BbvI GCAGC 1 cut(s) 469
BccI CCATC 1 cut(s) 536
BceAI ACGGC 1 cut(s) 384
BciT130I CCWGG 1 cut(s) 30
BfaI CTAG 2 cut(s) 102, 218
BglII AGATCT 1 cut(s) 80
BisI GCNGC 2 cut(s) 458, 479
BlsI GCNGC 2 cut(s) 459, 480
Bme1390I CCNGG 1 cut(s) 30
Bme18I GGWCC 1 cut(s) 98
BmgT120I GGNCC 1 cut(s) 98
BmiI GGNNCC 1 cut(s) 128
BmrFI CCNGG 1 cut(s) 30
BsaJI CCNNGG 1 cut(s) 583
Bsc4I CCNNNNNNNGG 1 cut(s) 259
BseBI CCWGG 1 cut(s) 30
BseDI CCNNGG 1 cut(s) 583
BseLI CCNNNNNNNGG 1 cut(s) 259
BseMII CTCAG 1 cut(s) 309
BseSI GKGCMC 2 cut(s) 8, 21
BseXI GCAGC 1 cut(s) 469
BsgI GTGCAG 1 cut(s) 563
BshFI GGCC 1 cut(s) 371
BshNI GGYRCC 1 cut(s) 126
BslI CCNNNNNNNGG 1 cut(s) 259
BsnI GGCC 1 cut(s) 371
Bsp1286I GDGCHC 2 cut(s) 8, 21
Bsp143I GATC 2 cut(s) 80, 514
BspACI CCGC 1 cut(s) 478
BspANI GGCC 1 cut(s) 371
BspCNI CTCAG 1 cut(s) 310
BspLI GGNNCC 1 cut(s) 128
BspPI GGATC 1 cut(s) 509
BspQI GCTCTTC 1 cut(s) 429
BspT107I GGYRCC 1 cut(s) 126
BssECI CCNNGG 1 cut(s) 583
BssMI GATC 2 cut(s) 80, 514
BssT1I CCWWGG 1 cut(s) 583
Bst2UI CCWGG 1 cut(s) 30
Bst4CI ACNGT 1 cut(s) 67
Bst6I CTCTTC 1 cut(s) 429
BstC8I GCNNGC 1 cut(s) 267
BstDEI CTNAG 1 cut(s) 318
BstKTI GATC 2 cut(s) 83, 517
BstMBI GATC 2 cut(s) 80, 514
BstMWI GCNNNNNNNGC 1 cut(s) 296
BstNI CCWGG 1 cut(s) 30
BstNSI RCATGY 1 cut(s) 269
BstSCI CCNGG 1 cut(s) 28
BstSLI GKGCMC 2 cut(s) 8, 21
BstV1I GCAGC 1 cut(s) 469
BstX2I RGATCY 1 cut(s) 80
BstYI RGATCY 1 cut(s) 80
BsuRI GGCC 1 cut(s) 371
BtsIMutI CAGTG 1 cut(s) 414
Cac8I GCNNGC 1 cut(s) 267
Cfr13I GGNCC 1 cut(s) 98
Csp6I GTAC 2 cut(s) 26, 127
CviAII CATG 3 cut(s) 266, 274, 422
CviJI RGCY 7 cut(s) 33, 250, 317, 371, 391, 460, 565
CviKI_1 RGCY 7 cut(s) 33, 250, 317, 371, 391, 460, 565
CviQI GTAC 2 cut(s) 26, 127
DdeI CTNAG 1 cut(s) 318
DpnI GATC 2 cut(s) 82, 516
DpnII GATC 2 cut(s) 80, 514
Eam1104I CTCTTC 1 cut(s) 429
EarI CTCTTC 1 cut(s) 429
Eco130I CCWWGG 1 cut(s) 583
Eco47I GGWCC 1 cut(s) 98
EcoO109I RGGNCCY 1 cut(s) 98
EcoRI GAATTC 1 cut(s) 221
EcoRII CCWGG 1 cut(s) 28
EcoT14I CCWWGG 1 cut(s) 583
ErhI CCWWGG 1 cut(s) 583
FaeI CATG 3 cut(s) 269, 277, 425
FatI CATG 3 cut(s) 265, 273, 421
Fnu4HI GCNGC 2 cut(s) 458, 479
Fsp4HI GCNGC 2 cut(s) 458, 479
FspBI CTAG 2 cut(s) 102, 218
GluI GCNGC 2 cut(s) 458, 479
HaeIII GGCC 1 cut(s) 371
Hin1II CATG 3 cut(s) 269, 277, 425
HincII GTYRAC 1 cut(s) 70
HindII GTYRAC 1 cut(s) 70
HphI GGTGA 1 cut(s) 34
Hpy166II GTNNAC 2 cut(s) 70, 306
Hpy188I TCNGA 1 cut(s) 80
Hpy188III TCNNGA 2 cut(s) 218, 431
Hpy8I GTNNAC 2 cut(s) 70, 306
HpyAV CCTTC 3 cut(s) 56, 253, 407
HpyCH4III ACNGT 1 cut(s) 67
HpyCH4V TGCA 6 cut(s) 200, 211, 269, 299, 457, 544
HpyF10VI GCNNNNNNNGC 1 cut(s) 296
HpyF3I CTNAG 1 cut(s) 318
Hsp92II CATG 3 cut(s) 269, 277, 425
KpnI GGTACC 1 cut(s) 130
Kzo9I GATC 2 cut(s) 80, 514
LguI GCTCTTC 1 cut(s) 429
LmnI GCTCC 3 cut(s) 255, 262, 530
LpnPI CCDG 4 cut(s) 15, 42, 285, 333
Lsp1109I GCAGC 1 cut(s) 469
MaeI CTAG 2 cut(s) 102, 218
MaeIII GTNAC 1 cut(s) 104
MalI GATC 2 cut(s) 82, 516
MboI GATC 2 cut(s) 80, 514
MboII GAAGA 1 cut(s) 446
MflI RGATCY 1 cut(s) 80
MhlI GDGCHC 2 cut(s) 8, 21
MluCI AATT 6 cut(s) 153, 201, 221, 256, 328, 519
MmeI TCCRAC 1 cut(s) 337
MnlI CCTC 4 cut(s) 50, 290, 382, 555
MseI TTAA 2 cut(s) 156, 327
MspA1I CMGCKG 1 cut(s) 317
MspR9I CCNGG 1 cut(s) 30
MvaI CCWGG 1 cut(s) 30
MwoI GCNNNNNNNGC 1 cut(s) 296
NdeII GATC 2 cut(s) 80, 514
NlaIII CATG 3 cut(s) 269, 277, 425
NlaIV GGNNCC 1 cut(s) 128
NspI RCATGY 1 cut(s) 269
PaeI GCATGC 1 cut(s) 269
PciSI GCTCTTC 1 cut(s) 429
PflMI CCANNNNNTGG 1 cut(s) 259
PkrI GCNGC 2 cut(s) 459, 480
PpuMI RGGWCCY 1 cut(s) 98
PshBI ATTAAT 1 cut(s) 327
Psp5II RGGWCCY 1 cut(s) 98
Psp6I CCWGG 1 cut(s) 28
PspGI CCWGG 1 cut(s) 28
PspN4I GGNNCC 1 cut(s) 128
PspPI GGNCC 1 cut(s) 98
PspPPI RGGWCCY 1 cut(s) 98
PsuI RGATCY 1 cut(s) 80
PvuII CAGCTG 1 cut(s) 317
RsaI GTAC 2 cut(s) 27, 128
RsaNI GTAC 2 cut(s) 26, 127
SapI GCTCTTC 1 cut(s) 429
SaqAI TTAA 2 cut(s) 156, 327
SatI GCNGC 2 cut(s) 458, 479
Sau3AI GATC 2 cut(s) 80, 514
Sau96I GGNCC 1 cut(s) 98
ScrFI CCNGG 1 cut(s) 30
SduI GDGCHC 2 cut(s) 8, 21
SinI GGWCC 1 cut(s) 98
SphI GCATGC 1 cut(s) 269
Sse9I AATT 6 cut(s) 153, 201, 221, 256, 328, 519
SsiI CCGC 1 cut(s) 478
SspMI CTAG 2 cut(s) 102, 218
StyD4I CCNGG 1 cut(s) 28
StyI CCWWGG 1 cut(s) 583
TaaI ACNGT 1 cut(s) 67
TasI AATT 6 cut(s) 153, 201, 221, 256, 328, 519
TauI GCSGC 1 cut(s) 481
Tru1I TTAA 2 cut(s) 156, 327
Tru9I TTAA 2 cut(s) 156, 327
TscAI CASTG 1 cut(s) 421
TseI GCWGC 1 cut(s) 457
TspDTI ATGAA 3 cut(s) 166, 221, 545
TspRI CASTG 1 cut(s) 421
Van91I CCANNNNNTGG 1 cut(s) 259
VpaK11BI GGWCC 1 cut(s) 98
VspI ATTAAT 1 cut(s) 327
XapI RAATTY 1 cut(s) 221
XbaI TCTAGA 1 cut(s) 217
XceI RCATGY 1 cut(s) 269
XspI CTAG 2 cut(s) 102, 218
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.