RchiOBHm_Chr6g0260141

Family of unknown function (DUF716)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0001745
Physical Location & Seq
Reverse (-)
15254619 .. 15255047
429 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ23327

Sequence Viewer

Length: 420 bp
ATGGGCACGTTCATTGGGCACACAGTACCTGGCTTTGCCCTCACCCTTCTTGGTTTATGGCACACTGTCAACACTATCAGATCTTATTTTCTCAAAGGCCCCAGTAACTTTGGAGTAAGGTTTTGGTACCCATTGAACTGCCCTTTTTCCAAAATTAAACACTTGGAACTCATTTTCATCTTATCTTTCTTTGTATTTGCAATTATTAAGAATTCCCTTTTCTTAACTTCTCCTTCAAGCTCCATAATTTGGAGCATGCAACCATGTTCCTCCACCTTGCCAGCTCAGTTTATTAATTTGTTTCAAATCCTATCTGGGGTCGTTGGATTGCTTATGGCCTCTGTTTTCTGCCAAGAGCTTTTCCTTATCCATTTCCACTCCACTGACCATGTTGGTCTTGAAGGGCATTACCATTGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF716

Protein Analysis

139

Amino Acids

15.77

Weight (kDa)

8.56

Isoelectric Point (pI)

35.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF716 PF04819 106 - 139 2e-08 Family of unknown function (DUF716)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 126
AccB1I GGYRCC 1 cut(s) 126
AccB7I CCANNNNNTGG 1 cut(s) 249
AcsI RAATTY 1 cut(s) 211
AfaI GTAC 2 cut(s) 27, 128
AfiI CCNNNNNNNGG 2 cut(s) 249, 316
AgsI TTSAA 4 cut(s) 136, 237, 305, 401
AjnI CCWGG 1 cut(s) 28
AluBI AGCT 3 cut(s) 240, 284, 358
AluI AGCT 3 cut(s) 240, 284, 358
AlwNI CAGNNNCTG 1 cut(s) 29
AoxI GGCC 2 cut(s) 97, 336
ApoI RAATTY 1 cut(s) 211
AseI ATTAAT 1 cut(s) 294
Asp718I GGTACC 1 cut(s) 126
AspS9I GGNCC 1 cut(s) 98
AsuHPI GGTGA 1 cut(s) 34
BaeGI GKGCMC 2 cut(s) 8, 21
BanI GGYRCC 1 cut(s) 126
BciT130I CCWGG 1 cut(s) 30
BglII AGATCT 1 cut(s) 80
Bme1390I CCNGG 1 cut(s) 30
BmgT120I GGNCC 1 cut(s) 98
BmiI GGNNCC 2 cut(s) 100, 128
BmrFI CCNGG 1 cut(s) 30
BmrI ACTGGG 1 cut(s) 96
BmuI ACTGGG 1 cut(s) 96
Bsc4I CCNNNNNNNGG 2 cut(s) 249, 316
Bse1I ACTGG 1 cut(s) 102
BseBI CCWGG 1 cut(s) 30
BseLI CCNNNNNNNGG 2 cut(s) 249, 316
BseMII CTCAG 1 cut(s) 299
BseNI ACTGG 1 cut(s) 102
BseSI GKGCMC 2 cut(s) 8, 21
BshFI GGCC 2 cut(s) 99, 338
BshNI GGYRCC 1 cut(s) 126
BslI CCNNNNNNNGG 2 cut(s) 249, 316
BsnI GGCC 2 cut(s) 99, 338
Bsp1286I GDGCHC 2 cut(s) 8, 21
Bsp143I GATC 1 cut(s) 80
BspANI GGCC 2 cut(s) 99, 338
BspCNI CTCAG 1 cut(s) 298
BspLI GGNNCC 2 cut(s) 100, 128
BspT107I GGYRCC 1 cut(s) 126
BsrI ACTGG 1 cut(s) 102
BssMI GATC 1 cut(s) 80
Bst2UI CCWGG 1 cut(s) 30
Bst4CI ACNGT 2 cut(s) 25, 67
BstC8I GCNNGC 2 cut(s) 257, 282
BstDEI CTNAG 1 cut(s) 285
BstKTI GATC 1 cut(s) 83
BstMBI GATC 1 cut(s) 80
BstNI CCWGG 1 cut(s) 30
BstNSI RCATGY 1 cut(s) 259
BstSCI CCNGG 1 cut(s) 28
BstSLI GKGCMC 2 cut(s) 8, 21
BstX2I RGATCY 1 cut(s) 80
BstYI RGATCY 1 cut(s) 80
BsuRI GGCC 2 cut(s) 99, 338
BtsIMutI CAGTG 2 cut(s) 63, 381
Cac8I GCNNGC 2 cut(s) 257, 282
CaiI CAGNNNCTG 1 cut(s) 29
Cfr13I GGNCC 1 cut(s) 98
Csp6I GTAC 2 cut(s) 26, 127
CviAII CATG 3 cut(s) 256, 264, 389
CviJI RGCY 6 cut(s) 33, 99, 240, 284, 338, 358
CviKI_1 RGCY 6 cut(s) 33, 99, 240, 284, 338, 358
CviQI GTAC 2 cut(s) 26, 127
DdeI CTNAG 1 cut(s) 285
DpnI GATC 1 cut(s) 82
DpnII GATC 1 cut(s) 80
EcoO109I RGGNCCY 1 cut(s) 98
EcoRI GAATTC 1 cut(s) 211
EcoRII CCWGG 1 cut(s) 28
FaeI CATG 3 cut(s) 259, 267, 392
FaiI YATR 6 cut(s) 58, 245, 257, 265, 335, 390
FatI CATG 3 cut(s) 255, 263, 388
HaeIII GGCC 2 cut(s) 99, 338
Hin1II CATG 3 cut(s) 259, 267, 392
HincII GTYRAC 1 cut(s) 70
HindII GTYRAC 1 cut(s) 70
HphI GGTGA 1 cut(s) 34
Hpy166II GTNNAC 1 cut(s) 70
Hpy188I TCNGA 1 cut(s) 80
Hpy188III TCNNGA 1 cut(s) 398
Hpy8I GTNNAC 1 cut(s) 70
HpyAV CCTTC 3 cut(s) 56, 243, 395
HpyCH4III ACNGT 2 cut(s) 25, 67
HpyCH4IV ACGT 1 cut(s) 8
HpyCH4V TGCA 2 cut(s) 200, 259
HpyF3I CTNAG 1 cut(s) 285
HpySE526I ACGT 1 cut(s) 8
Hsp92II CATG 3 cut(s) 259, 267, 392
KpnI GGTACC 1 cut(s) 130
Kzo9I GATC 1 cut(s) 80
LmnI GCTCC 2 cut(s) 245, 252
LpnPI CCDG 5 cut(s) 15, 42, 115, 294, 300
MaeII ACGT 1 cut(s) 8
MaeIII GTNAC 1 cut(s) 104
MalI GATC 1 cut(s) 82
MboI GATC 1 cut(s) 80
MflI RGATCY 1 cut(s) 80
MhlI GDGCHC 2 cut(s) 8, 21
MluCI AATT 5 cut(s) 153, 201, 211, 246, 295
MmeI TCCRAC 1 cut(s) 304
MnlI CCTC 3 cut(s) 50, 280, 349
MseI TTAA 4 cut(s) 156, 207, 224, 294
MspR9I CCNGG 1 cut(s) 30
MvaI CCWGG 1 cut(s) 30
NdeII GATC 1 cut(s) 80
NlaIII CATG 3 cut(s) 259, 267, 392
NlaIV GGNNCC 2 cut(s) 100, 128
NspI RCATGY 1 cut(s) 259
PaeI GCATGC 1 cut(s) 259
PflMI CCANNNNNTGG 1 cut(s) 249
PshBI ATTAAT 1 cut(s) 294
Psp6I CCWGG 1 cut(s) 28
PspGI CCWGG 1 cut(s) 28
PspN4I GGNNCC 2 cut(s) 100, 128
PspPI GGNCC 1 cut(s) 98
PstNI CAGNNNCTG 1 cut(s) 29
PsuI RGATCY 1 cut(s) 80
RsaI GTAC 2 cut(s) 27, 128
RsaNI GTAC 2 cut(s) 26, 127
SaqAI TTAA 4 cut(s) 156, 207, 224, 294
Sau3AI GATC 1 cut(s) 80
Sau96I GGNCC 1 cut(s) 98
ScrFI CCNGG 1 cut(s) 30
SduI GDGCHC 2 cut(s) 8, 21
SetI ASST 7 cut(s) 11, 31, 122, 242, 278, 286, 360
SphI GCATGC 1 cut(s) 259
Sse9I AATT 5 cut(s) 153, 201, 211, 246, 295
StyD4I CCNGG 1 cut(s) 28
TaaI ACNGT 2 cut(s) 25, 67
TaiI ACGT 1 cut(s) 11
TasI AATT 5 cut(s) 153, 201, 211, 246, 295
Tru1I TTAA 4 cut(s) 156, 207, 224, 294
Tru9I TTAA 4 cut(s) 156, 207, 224, 294
TscAI CASTG 2 cut(s) 70, 388
TspDTI ATGAA 1 cut(s) 166
TspRI CASTG 2 cut(s) 70, 388
Van91I CCANNNNNTGG 1 cut(s) 249
VspI ATTAAT 1 cut(s) 294
XapI RAATTY 1 cut(s) 211
XceI RCATGY 1 cut(s) 259
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.