Rorug05G0591700

Family of unknown function (DUF716)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0001745
Physical Location & Seq
Reverse (-)
78956739 .. 78958238
1500 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0591700.1

Sequence Viewer

Length: 1500 bp
ATGTCAGATCCTTATTTTGTTGGCCGCTTTTTGTGGCTACAGAGAAATAACCAAACCCTAATACCTGGCACTATTATAAGTAATAAGGCCGAAGAACTTGCCACAAACGACGTGCTGGCAAGATTAAGGAATTTCTTCAAACTCAAGAAGAAGAAATTACAAGTGCTAGCTACATGGAATGACTTGGTTCTCTGCTGCACAGTCAGCGGAGGCTATTGCTATTACTACGTCTGCAATCCACGGACCATGCAACTGGTTCATCTTCCTCGCATCGCTCAATACCCTGGTCTCCCTGATATATTCGACTATCAGCCTGTGGGATTCATCTGTGAGCCCTACAATAAGGAAGACAGGGTCGTCGTCCACAGTGATCAACAACAAGAACAAAAAGAAGAAAGGAAGGTAATTAAAATTAATGCCGAGTACAAGTGTAAGGTTGTCAGAATTCTTCCTCCTGATGAGATAATAAAACCTTGTTTCGAATTCAAGATTCAGGTATTCTCTAGTGAGACTGGTGAATGGAGAGAATCACTAGTGGCATGTCCACAAGGCTTTATTTATAAACAACTCGGGGATGTATCTGTCTGTGCTACCAATGGCATGCTATATTGGAATAATGCTGACTATGGTGATCTGCTCGTGCTGGAACCGTTGATCAACAACAAGAGTACTACTACTACTGCCAATGATGATTCTGGCTATCAATTTCGTGTTATTAAATATGTTCAGGAAGATGATGTTCCTTATCCTCATCAGCCATTTTCGCCTTATCACCTTGGCGGGCAGCAGGGACGTCTGCTGTTGGTGCGTACAGTGGCGAATGGATTCCCCGACATGGGCGCTAGGCTATTCATTTGGGAGTTGGATCAAGAAAGGGGTGGAGAACTCTGTCGTCGGAGGACTTACAACATACTGGACAAAGAATTTCTTGGTTTAGAGACTGGGGTGTCTTGTATAGGTTTCGACCAAAATGATTTGGATATATGTTATGTACTTGTATGTCATCACGAGGAACTAGAAGGCCTTCACAAGTACAACCTTCACACAGGGAAACGGTCCATGATAAACAAAGAATTTTATGGGTGCGCATTACAAGTTATGCTTCCGTGGTGGTCAGTTCCTACAATATCCGATCCCATGCCGGATAGGAATACCGAGATCAACGGGAGTGAGGAAGAAGGTGCCGCGCTCAGATATATAGCAAGATCAAAAGTGAAGAAAACCAAAGATGTGGATGGTGTTGTGAATGGAGAGGAGGGTGCGGGTTTTCAAGCTCCAAGGTTGAGTGGTAGAAAGCACGGCATTGATGTGGAGAAGAGTGGTGCAACAAAGAGGTTCAAGGCGATGGATGTAGTAGTCCCAGCTAATGCTCCTAAGGAAGTGCATGCTTCAAATCCCACTTCGTCCAAGGTATCACGAGAAACCACCACACGAGAAATTCTTCATCATGCTTTTGGAAACATGCCAATATTCCTTAAAAATGCTTCAAGTTCGTGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF716

Protein Analysis

499

Amino Acids

56.69

Weight (kDa)

6.65

Isoelectric Point (pI)

41.16

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 77, 561
AasI GACNNNNNNGTC 2 cut(s) 356, 946
AatII GACGTC 1 cut(s) 796
Acc16I TGCGCA 1 cut(s) 1087
AccB1I GGYRCC 1 cut(s) 1181
AccII CGCG 1 cut(s) 1187
AciI CCGC 5 cut(s) 25, 207, 780, 1185, 1262
AclWI GGATC 3 cut(s) 2, 873, 1127
AcoI YGGCCR 1 cut(s) 22
AcsI RAATTY 6 cut(s) 130, 444, 482, 923, 1073, 1437
AcyI GRCGYC 1 cut(s) 793
AfaI GTAC 5 cut(s) 425, 670, 811, 993, 1034
AfiI CCNNNNNNNGG 2 cut(s) 835, 836
AgsI TTSAA 6 cut(s) 139, 487, 1271, 1339, 1392, 1488
AhlI ACTAGT 1 cut(s) 532
AjiI CACGTC 1 cut(s) 112
AjnI CCWGG 2 cut(s) 64, 283
AluBI AGCT 3 cut(s) 170, 1274, 1364
AluI AGCT 3 cut(s) 170, 1274, 1364
Alw26I GTCTC 3 cut(s) 293, 503, 932
AlwI GGATC 3 cut(s) 2, 873, 1127
Ama87I CYCGRG 1 cut(s) 569
AoxI GGCC 3 cut(s) 22, 87, 1021
ApeKI GCWGC 2 cut(s) 195, 784
ApoI RAATTY 6 cut(s) 130, 444, 482, 923, 1073, 1437
AseI ATTAAT 1 cut(s) 414
Asp700I GAANNNNTTC 4 cut(s) 134, 824, 1023, 1440
AspLEI GCGC 3 cut(s) 842, 1088, 1189
AspS9I GGNCC 2 cut(s) 243, 1056
AsuHPI GGTGA 3 cut(s) 527, 641, 764
AsuII TTCGAA 1 cut(s) 480
AsuNHI GCTAGC 1 cut(s) 166
AvaI CYCGRG 1 cut(s) 569
AvaII GGWCC 2 cut(s) 243, 1056
AxyI CCTNAGG 1 cut(s) 1374
BanI GGYRCC 1 cut(s) 1181
BanII GRGCYC 1 cut(s) 336
BauI CACGAG 4 cut(s) 638, 1007, 1416, 1431
BbsI GAAGAC 1 cut(s) 354
BbvI GCAGC 2 cut(s) 182, 796
BccI CCATC 2 cut(s) 1229, 1339
BceAI ACGGC 1 cut(s) 1315
BcgI CGANNNNNNTGC 2 cut(s) 80, 114
BciT130I CCWGG 2 cut(s) 66, 285
BclI TGATCA 2 cut(s) 370, 654
BcoDI GTCTC 3 cut(s) 293, 503, 932
BcuI ACTAGT 1 cut(s) 532
BfaI CTAG 5 cut(s) 167, 504, 533, 843, 1016
BfmI CTRYAG 1 cut(s) 38
BfoI RGCGCY 1 cut(s) 843
BisI GCNGC 4 cut(s) 25, 196, 785, 1185
BlsI GCNGC 4 cut(s) 26, 197, 786, 1186
BmcAI AGTACT 1 cut(s) 670
Bme1390I CCNGG 2 cut(s) 66, 285
Bme18I GGWCC 2 cut(s) 243, 1056
BmeT110I CYCGRG 1 cut(s) 569
BmgBI CACGTC 1 cut(s) 112
BmgT120I GGNCC 2 cut(s) 243, 1056
BmiI GGNNCC 2 cut(s) 648, 1183
BmrFI CCNGG 2 cut(s) 66, 285
BmrI ACTGGG 1 cut(s) 951
BmsI GCATC 1 cut(s) 279
BmtI GCTAGC 1 cut(s) 170
BmuI ACTGGG 1 cut(s) 951
BpiI GAAGAC 1 cut(s) 354
Bpu14I TTCGAA 1 cut(s) 480
BpuEI CTTGAG 1 cut(s) 128
BsaHI GRCGYC 1 cut(s) 793
BsaI GGTCTC 1 cut(s) 293
BsaJI CCNNGG 6 cut(s) 239, 283, 775, 1106, 1277, 1407
Bsc4I CCNNNNNNNGG 2 cut(s) 835, 836
Bse1I ACTGG 4 cut(s) 258, 517, 918, 946
Bse21I CCTNAGG 1 cut(s) 1374
BseBI CCWGG 2 cut(s) 66, 285
BseDI CCNNGG 6 cut(s) 239, 283, 775, 1106, 1277, 1407
BseGI GGATG 3 cut(s) 580, 1240, 1354
BseLI CCNNNNNNNGG 2 cut(s) 835, 836
BseMII CTCAG 1 cut(s) 1204
BseNI ACTGG 4 cut(s) 258, 517, 918, 946
BseRI GAGGAG 1 cut(s) 1268
BseXI GCAGC 2 cut(s) 182, 796
BseYI CCCAGC 1 cut(s) 1360
BsgI GTGCAG 1 cut(s) 181
Bsh1236I CGCG 1 cut(s) 1187
BshFI GGCC 3 cut(s) 24, 89, 1023
BshNI GGYRCC 1 cut(s) 1181
BsiHKCI CYCGRG 1 cut(s) 569
BsiSI CCGG 1 cut(s) 1142
BslFI GGGAC 2 cut(s) 804, 1343
BslI CCNNNNNNNGG 2 cut(s) 835, 836
BsmAI GTCTC 3 cut(s) 293, 503, 932
BsmFI GGGAC 2 cut(s) 804, 1343
BsnI GGCC 3 cut(s) 24, 89, 1023
Bso31I GGTCTC 1 cut(s) 293
BsoBI CYCGRG 1 cut(s) 569
Bsp119I TTCGAA 1 cut(s) 480
Bsp1286I GDGCHC 1 cut(s) 336
Bsp143I GATC 8 cut(s) 7, 370, 631, 654, 865, 1132, 1158, 1205
BspACI CCGC 5 cut(s) 25, 207, 780, 1185, 1262
BspANI GGCC 3 cut(s) 24, 89, 1023
BspCNI CTCAG 1 cut(s) 1203
BspFNI CGCG 1 cut(s) 1187
BspLI GGNNCC 2 cut(s) 648, 1183
BspOI GCTAGC 1 cut(s) 170
BspPI GGATC 3 cut(s) 2, 873, 1127
BspT104I TTCGAA 1 cut(s) 480
BspT107I GGYRCC 1 cut(s) 1181
BspTNI GGTCTC 1 cut(s) 293
BsrI ACTGG 4 cut(s) 258, 517, 918, 946
BssECI CCNNGG 6 cut(s) 239, 283, 775, 1106, 1277, 1407
BssMI GATC 8 cut(s) 7, 370, 631, 654, 865, 1132, 1158, 1205
BssNI GRCGYC 1 cut(s) 793
BssSI CACGAG 4 cut(s) 638, 1007, 1416, 1431
BssT1I CCWWGG 3 cut(s) 775, 1277, 1407
Bst2BI CACGAG 4 cut(s) 638, 1007, 1416, 1431
Bst2UI CCWGG 2 cut(s) 66, 285
Bst4CI ACNGT 5 cut(s) 202, 368, 651, 814, 1056
Bst6I CTCTTC 1 cut(s) 1310
BstACI GRCGYC 1 cut(s) 793
BstBI TTCGAA 1 cut(s) 480
BstC8I GCNNGC 5 cut(s) 117, 168, 602, 782, 1386
BstDEI CTNAG 2 cut(s) 1190, 1374
BstDSI CCRYGG 2 cut(s) 239, 1106
BstF5I GGATG 3 cut(s) 580, 1240, 1354
BstFNI CGCG 1 cut(s) 1187
BstH2I RGCGCY 1 cut(s) 843
BstHHI GCGC 3 cut(s) 842, 1088, 1189
BstKTI GATC 8 cut(s) 10, 373, 634, 657, 868, 1135, 1161, 1208
BstMAI GTCTC 3 cut(s) 293, 503, 932
BstMBI GATC 8 cut(s) 7, 370, 631, 654, 865, 1132, 1158, 1205
BstMWI GCNNNNNNNGC 3 cut(s) 204, 763, 805
BstNI CCWGG 2 cut(s) 66, 285
BstNSI RCATGY 4 cut(s) 543, 604, 1388, 1465
BstSCI CCNGG 2 cut(s) 64, 283
BstSFI CTRYAG 1 cut(s) 38
BstUI CGCG 1 cut(s) 1187
BstV1I GCAGC 2 cut(s) 182, 796
BstV2I GAAGAC 1 cut(s) 354
BstX2I RGATCY 1 cut(s) 7
BstXI CCANNNNNNTGG 2 cut(s) 253, 1231
BstYI RGATCY 1 cut(s) 7
Bsu36I CCTNAGG 1 cut(s) 1374
BsuRI GGCC 3 cut(s) 24, 89, 1023
BtgI CCRYGG 2 cut(s) 239, 1106
BtgZI GCGATG 2 cut(s) 256, 1358
BtrI CACGTC 1 cut(s) 112
BtsCI GGATG 3 cut(s) 580, 1240, 1354
BtsIMutI CAGTG 2 cut(s) 373, 819
Cac8I GCNNGC 5 cut(s) 117, 168, 602, 782, 1386
CfoI GCGC 3 cut(s) 842, 1088, 1189
Cfr13I GGNCC 2 cut(s) 243, 1056
Csp6I GTAC 5 cut(s) 424, 669, 810, 992, 1033
CviQI GTAC 5 cut(s) 424, 669, 810, 992, 1033
DdeI CTNAG 2 cut(s) 1190, 1374
DpnI GATC 8 cut(s) 9, 372, 633, 656, 867, 1134, 1160, 1207
DpnII GATC 8 cut(s) 7, 370, 631, 654, 865, 1132, 1158, 1205
DrdI GACNNNNNNGTC 2 cut(s) 356, 946
DseDI GACNNNNNNGTC 2 cut(s) 356, 946
EaeI YGGCCR 1 cut(s) 22
Eam1104I CTCTTC 1 cut(s) 1310
EarI CTCTTC 1 cut(s) 1310
Eco130I CCWWGG 3 cut(s) 775, 1277, 1407
Eco147I AGGCCT 1 cut(s) 1023
Eco24I GRGCYC 1 cut(s) 336
Eco31I GGTCTC 1 cut(s) 293
Eco47I GGWCC 2 cut(s) 243, 1056
Eco81I CCTNAGG 1 cut(s) 1374
Eco88I CYCGRG 1 cut(s) 569
EcoRI GAATTC 2 cut(s) 444, 482
EcoRII CCWGG 2 cut(s) 64, 283
EcoT14I CCWWGG 3 cut(s) 775, 1277, 1407
EcoT38I GRGCYC 1 cut(s) 336
ErhI CCWWGG 3 cut(s) 775, 1277, 1407
FalI AAGNNNNNCTT 4 cut(s) 912, 944, 1086, 1118
FaqI GGGAC 2 cut(s) 804, 1343
FauI CCCGC 2 cut(s) 773, 1255
FbaI TGATCA 2 cut(s) 370, 654
Fnu4HI GCNGC 4 cut(s) 25, 196, 785, 1185
FokI GGATG 3 cut(s) 587, 1247, 1361
FriOI GRGCYC 1 cut(s) 336
Fsp4HI GCNGC 4 cut(s) 25, 196, 785, 1185
FspAI RTGCGCAY 1 cut(s) 1087
FspBI CTAG 5 cut(s) 167, 504, 533, 843, 1016
FspI TGCGCA 1 cut(s) 1087
GlaI GCGC 3 cut(s) 841, 1087, 1188
GluI GCNGC 4 cut(s) 25, 196, 785, 1185
GsaI CCCAGC 1 cut(s) 1364
HaeII RGCGCY 1 cut(s) 843
HaeIII GGCC 3 cut(s) 24, 89, 1023
HapII CCGG 1 cut(s) 1142
HhaI GCGC 3 cut(s) 842, 1088, 1189
Hin1I GRCGYC 1 cut(s) 793
Hin6I GCGC 3 cut(s) 840, 1086, 1187
HinP1I GCGC 3 cut(s) 840, 1086, 1187
HinfI GANTC 5 cut(s) 321, 490, 527, 692, 825
HpaII CCGG 1 cut(s) 1142
HphI GGTGA 3 cut(s) 527, 641, 764
Hpy166II GTNNAC 2 cut(s) 364, 545
Hpy188I TCNGA 5 cut(s) 7, 443, 897, 1132, 1193
Hpy188III TCNNGA 7 cut(s) 145, 455, 487, 728, 869, 1007, 1416
Hpy8I GTNNAC 2 cut(s) 364, 545
Hpy99I CGWCG 3 cut(s) 113, 362, 897
HpyAV CCTTC 5 cut(s) 394, 1013, 1034, 1049, 1172
HpyCH4III ACNGT 5 cut(s) 202, 368, 651, 814, 1056
HpyCH4IV ACGT 3 cut(s) 111, 228, 793
HpyCH4V TGCA 5 cut(s) 198, 234, 250, 1325, 1384
HpyF10VI GCNNNNNNNGC 3 cut(s) 204, 763, 805
HpyF3I CTNAG 2 cut(s) 1190, 1374
HpySE526I ACGT 3 cut(s) 111, 228, 793
Hsp92I GRCGYC 1 cut(s) 793
HspAI GCGC 3 cut(s) 840, 1086, 1187
Ksp22I TGATCA 2 cut(s) 370, 654
Kzo9I GATC 8 cut(s) 7, 370, 631, 654, 865, 1132, 1158, 1205
LmnI GCTCC 2 cut(s) 1279, 1375
Lsp1109I GCAGC 2 cut(s) 182, 796
LweI GCATC 1 cut(s) 279
MaeI CTAG 5 cut(s) 167, 504, 533, 843, 1016
MaeII ACGT 3 cut(s) 111, 228, 793
MalI GATC 8 cut(s) 9, 372, 633, 656, 867, 1134, 1160, 1207
MboI GATC 8 cut(s) 7, 370, 631, 654, 865, 1132, 1158, 1205
MflI RGATCY 1 cut(s) 7
MhlI GDGCHC 1 cut(s) 336
MmeI TCCRAC 2 cut(s) 843, 875
MroXI GAANNNNTTC 4 cut(s) 134, 824, 1023, 1440
MseI TTAA 6 cut(s) 125, 408, 414, 717, 1476, 1498
MslI CAYNNNNRTG 1 cut(s) 1307
MspA1I CMGCKG 1 cut(s) 207
MspI CCGG 1 cut(s) 1142
MspR9I CCNGG 2 cut(s) 66, 285
MvaI CCWGG 2 cut(s) 66, 285
MvnI CGCG 1 cut(s) 1187
MwoI GCNNNNNNNGC 3 cut(s) 204, 763, 805
NdeII GATC 8 cut(s) 7, 370, 631, 654, 865, 1132, 1158, 1205
NheI GCTAGC 1 cut(s) 166
NlaIV GGNNCC 2 cut(s) 648, 1183
NmeAIII GCCGAG 1 cut(s) 445
NsbI TGCGCA 1 cut(s) 1087
NspI RCATGY 4 cut(s) 543, 604, 1388, 1465
NspV TTCGAA 1 cut(s) 480
PaeI GCATGC 2 cut(s) 604, 1388
PceI AGGCCT 1 cut(s) 1023
PdmI GAANNNNTTC 4 cut(s) 134, 824, 1023, 1440
PfeI GAWTC 5 cut(s) 321, 490, 527, 692, 825
PflFI GACNNNGTC 1 cut(s) 353
PkrI GCNGC 4 cut(s) 26, 197, 786, 1186
PshBI ATTAAT 1 cut(s) 414
PsiI TTATAA 2 cut(s) 77, 561
Psp6I CCWGG 2 cut(s) 64, 283
PspFI CCCAGC 1 cut(s) 1360
PspGI CCWGG 2 cut(s) 64, 283
PspN4I GGNNCC 2 cut(s) 648, 1183
PspPI GGNCC 2 cut(s) 243, 1056
PsuI RGATCY 1 cut(s) 7
PsyI GACNNNGTC 1 cut(s) 353
RsaI GTAC 5 cut(s) 425, 670, 811, 993, 1034
RsaNI GTAC 5 cut(s) 424, 669, 810, 992, 1033
RseI CAYNNNNRTG 1 cut(s) 1307
SaqAI TTAA 6 cut(s) 125, 408, 414, 717, 1476, 1498
SatI GCNGC 4 cut(s) 25, 196, 785, 1185
Sau3AI GATC 8 cut(s) 7, 370, 631, 654, 865, 1132, 1158, 1205
Sau96I GGNCC 2 cut(s) 243, 1056
ScaI AGTACT 1 cut(s) 670
ScrFI CCNGG 2 cut(s) 66, 285
SduI GDGCHC 1 cut(s) 336
SfaNI GCATC 1 cut(s) 279
SfcI CTRYAG 1 cut(s) 38
SfuI TTCGAA 1 cut(s) 480
SinI GGWCC 2 cut(s) 243, 1056
SmiMI CAYNNNNRTG 1 cut(s) 1307
SmlI CTYRAG 1 cut(s) 143
SmoI CTYRAG 1 cut(s) 143
SpeI ACTAGT 1 cut(s) 532
SphI GCATGC 2 cut(s) 604, 1388
SseBI AGGCCT 1 cut(s) 1023
SsiI CCGC 5 cut(s) 25, 207, 780, 1185, 1262
SspI AATATT 1 cut(s) 1470
SspMI CTAG 5 cut(s) 167, 504, 533, 843, 1016
StuI AGGCCT 1 cut(s) 1023
StyD4I CCNGG 2 cut(s) 64, 283
StyI CCWWGG 3 cut(s) 775, 1277, 1407
TaaI ACNGT 5 cut(s) 202, 368, 651, 814, 1056
TaiI ACGT 3 cut(s) 114, 231, 796
TaqI TCGA 3 cut(s) 303, 480, 963
TatI WGTACW 4 cut(s) 423, 668, 991, 1032
TauI GCSGC 2 cut(s) 27, 1187
TfiI GAWTC 5 cut(s) 321, 490, 527, 692, 825
Tru1I TTAA 6 cut(s) 125, 408, 414, 717, 1476, 1498
Tru9I TTAA 6 cut(s) 125, 408, 414, 717, 1476, 1498
TscAI CASTG 2 cut(s) 373, 819
TseI GCWGC 2 cut(s) 195, 784
TspDTI ATGAA 4 cut(s) 248, 313, 841, 1433
TspGWI ACGGA 2 cut(s) 256, 1095
TspRI CASTG 2 cut(s) 373, 819
Tth111I GACNNNGTC 1 cut(s) 353
VpaK11BI GGWCC 2 cut(s) 243, 1056
VspI ATTAAT 1 cut(s) 414
XapI RAATTY 6 cut(s) 130, 444, 482, 923, 1073, 1437
XceI RCATGY 4 cut(s) 543, 604, 1388, 1465
XmnI GAANNNNTTC 4 cut(s) 134, 824, 1023, 1440
XspI CTAG 5 cut(s) 167, 504, 533, 843, 1016
ZraI GACGTC 1 cut(s) 794
ZrmI AGTACT 1 cut(s) 670
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.