AT3G27570

Thioredoxin fold (InterPro IPR012335), Sucraseferredoxin-like (InterPro IPR009737), Thioredoxin-like fold (InterPro IPR012336)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0001205
Physical Location & Seq
Reverse (-)
10213996 .. 10216964
2969 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G27570.2

Sequence Viewer

Length: 1140 bp
ATGATAACGAAATTTACATCTCCATTTCGATTGATTCGCTTCTTCAACTCTCTCTCTTCTTCTTCTTCTATTTGCTTCTGTGAAACCCTAGATTTTGGTGTGAGGAAGAAGACTTCAATGGCGGAAGAAGTAGCAGAGAACGTTAACGCAGTCCCGGTTTCGGAAGACGAGCTTTACGGATTTAAACGATCGGAGATGTACAGTGGTACTCTCGCCGGTTCCGTTGGTCCGTACGGTCGTCATGTTTTCCTTTGTTACAAGAGTCATGAGACTTGGCTTCCTCGTGTTGAAACTGAAGGACTTCCTCAGCGTTTCGCGAAGTTGTTTAAGGATCGTAAAGCTGATTTCGCTGTTGAGACGAAGCTTACGGTGTGTGGTGGTGGTGGTGAATCTGATGGAGATGTGTTGATTTTCCCGGAGATGGTTAGATACAAGGCGATTCAGGACACAGATGTGGATGCTTTTGTGGAAGATGTGCTTGTGAAAGGAAAAACATGGACTTCTGGGATTCAGGAAGAGTTGACTGGTTCCTTTGTGTTTGTGTGCGCTCATGGTAGCCGAGACAAGAGATGTGGTGTTTGTGGACCAGTTCTTATGGAGAAGTTCGAGCAGGAGATAAGTTCACGTGGACTTTCAGACAAAATCTTTGTTTTGCCGTGTTCTCATATCGGTGGGCACAAGTATGCTGGAAATTTGATTGTCTTCAGCCCTGATTCAGCTGGAAATGTTTCTGGCCATTGGTATGGCTATGTGACTCCTGATGACGTGCCTGCAATGCTTGATCAGCATATTGCAAAAGGAGAAATCATTCAAAACCTTTCCAGAGGCCAGATGAGACTAAGACCCGAGGGTGAGGAAGCTGAGAAAGAGGATGAGCATAAAATCCCAAACGGAAACAGTGTAATGGTGGAAGAACGCGAACCTGTGGAGCAGAAAGGGTTCACAGGAGGTTGTTGCCAAGGTGCAAACGGAGTTTCATGTTGCCAAGAACAAGCTGCAGAGCCAGTCAAGAAAGAAGGATGCATGAAGCTAAACTGGTTGAAATCGATGGGGAAAGAAGAGGTTCTCTTGGGAGCAGCAGTAGTTTCAGCTGTCGCAACCGTTGCCGTGGCTTACAGCATTTACAGAAGGTCAGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Suc_Fer-like

Protein Analysis

379

Amino Acids

41.61

Weight (kDa)

5.54

Isoelectric Point (pI)

38.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Suc_Fer-like PF06999 32 - 237 2.1e-43 Sucrase/ferredoxin-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 317, 918
AciI CCGC 1 cut(s) 122
AclI AACGTT 1 cut(s) 141
AclWI GGATC 1 cut(s) 339
AcoI YGGCCR 1 cut(s) 733
AcsI RAATTY 2 cut(s) 11, 691
AcuI CTGAAG 2 cut(s) 315, 688
AcvI CACGTG 1 cut(s) 626
AfaI GTAC 3 cut(s) 200, 208, 233
AfiI CCNNNNNNNGG 2 cut(s) 160, 421
AgsI TTSAA 5 cut(s) 46, 117, 290, 812, 1042
AjiI CACGTC 1 cut(s) 766
AleI CACNNNNGTG 1 cut(s) 452
AluBI AGCT 8 cut(s) 172, 341, 364, 719, 860, 995, 1030, 1091
AluI AGCT 8 cut(s) 172, 341, 364, 719, 860, 995, 1030, 1091
Alw26I GTCTC 4 cut(s) 263, 350, 555, 829
AlwI GGATC 1 cut(s) 339
Ama87I CYCGRG 1 cut(s) 845
AoxI GGCC 2 cut(s) 733, 826
ApeKI GCWGC 2 cut(s) 995, 1076
ApoI RAATTY 2 cut(s) 11, 691
ArsI GACNNNNNNTTYG 2 cut(s) 629, 661
Asp700I GAANNNNTTC 5 cut(s) 300, 727, 807, 938, 1062
AspLEI GCGC 1 cut(s) 548
AspS9I GGNCC 2 cut(s) 227, 584
AsuC2I CCSGG 2 cut(s) 155, 416
AsuHPI GGTGA 2 cut(s) 398, 863
AvaI CYCGRG 1 cut(s) 845
AvaII GGWCC 2 cut(s) 227, 584
BaeGI GKGCMC 1 cut(s) 678
BalI TGGCCA 1 cut(s) 735
BauI CACGAG 1 cut(s) 282
BbrPI CACGTG 1 cut(s) 626
BbsI GAAGAC 3 cut(s) 116, 171, 694
BbvCI CCTCAGC 1 cut(s) 306
BbvI GCAGC 2 cut(s) 982, 1088
BccI CCATC 3 cut(s) 389, 415, 1042
BceAI ACGGC 2 cut(s) 640, 1091
BclI TGATCA 1 cut(s) 781
BcnI CCSGG 2 cut(s) 155, 416
BcoDI GTCTC 4 cut(s) 263, 350, 555, 829
BfaI CTAG 1 cut(s) 89
BfmI CTRYAG 1 cut(s) 996
BisI GCNGC 2 cut(s) 996, 1077
BlsI GCNGC 2 cut(s) 997, 1078
Bme1390I CCNGG 2 cut(s) 155, 416
Bme18I GGWCC 2 cut(s) 227, 584
BmeT110I CYCGRG 1 cut(s) 845
BmgBI CACGTC 1 cut(s) 766
BmgT120I GGNCC 2 cut(s) 227, 584
BmiI GGNNCC 2 cut(s) 220, 529
BmrFI CCNGG 2 cut(s) 155, 416
BmsI GCATC 2 cut(s) 448, 1010
BpiI GAAGAC 3 cut(s) 116, 171, 694
Bpu10I CCTNAGC 1 cut(s) 306
BpuMI CCSGG 2 cut(s) 155, 416
Bsa29I ATCGAT 1 cut(s) 1046
BsaAI YACGTR 1 cut(s) 626
BsaJI CCNNGG 3 cut(s) 846, 958, 1107
BsaXI ACNNNNNCTCC 2 cut(s) 1065, 1095
Bsc4I CCNNNNNNNGG 2 cut(s) 160, 421
Bse118I RCCGGY 1 cut(s) 215
Bse1I ACTGG 4 cut(s) 529, 587, 1004, 1040
Bse3DI GCAATG 1 cut(s) 780
BseCI ATCGAT 1 cut(s) 1046
BseDI CCNNGG 3 cut(s) 846, 958, 1107
BseGI GGATG 3 cut(s) 463, 877, 1025
BseLI CCNNNNNNNGG 2 cut(s) 160, 421
BseMI GCAATG 1 cut(s) 780
BseMII CTCAG 2 cut(s) 320, 852
BseNI ACTGG 4 cut(s) 529, 587, 1004, 1040
BseSI GKGCMC 1 cut(s) 678
BseXI GCAGC 2 cut(s) 982, 1088
Bsh1236I CGCG 2 cut(s) 317, 918
Bsh1285I CGRYCG 2 cut(s) 191, 238
BshFI GGCC 2 cut(s) 735, 828
BshVI ATCGAT 1 cut(s) 1046
BsiEI CGRYCG 2 cut(s) 191, 238
BsiHKCI CYCGRG 1 cut(s) 845
BsiSI CCGG 3 cut(s) 155, 216, 416
BsiWI CGTACG 1 cut(s) 231
BslFI GGGAC 1 cut(s) 137
BslI CCNNNNNNNGG 2 cut(s) 160, 421
BsmAI GTCTC 4 cut(s) 263, 350, 555, 829
BsmBI CGTCTC 1 cut(s) 350
BsmFI GGGAC 1 cut(s) 137
BsnI GGCC 2 cut(s) 735, 828
BsoBI CYCGRG 1 cut(s) 845
Bsp1286I GDGCHC 1 cut(s) 678
Bsp1407I TGTACA 1 cut(s) 198
Bsp143I GATC 3 cut(s) 188, 331, 781
Bsp68I TCGCGA 1 cut(s) 317
BspACI CCGC 1 cut(s) 122
BspANI GGCC 2 cut(s) 735, 828
BspCNI CTCAG 2 cut(s) 319, 853
BspDI ATCGAT 1 cut(s) 1046
BspFNI CGCG 2 cut(s) 317, 918
BspHI TCATGA 1 cut(s) 265
BspLI GGNNCC 2 cut(s) 220, 529
BspMAI CTGCAG 1 cut(s) 1000
BspPI GGATC 1 cut(s) 339
BsrDI GCAATG 1 cut(s) 780
BsrFI RCCGGY 1 cut(s) 215
BsrGI TGTACA 1 cut(s) 198
BsrI ACTGG 4 cut(s) 529, 587, 1004, 1040
BssAI RCCGGY 1 cut(s) 215
BssECI CCNNGG 3 cut(s) 846, 958, 1107
BssMI GATC 3 cut(s) 188, 331, 781
BssSI CACGAG 1 cut(s) 282
BssT1I CCWWGG 1 cut(s) 958
Bst2BI CACGAG 1 cut(s) 282
Bst4CI ACNGT 5 cut(s) 203, 236, 370, 899, 1102
Bst6I CTCTTC 3 cut(s) 61, 510, 1053
BstAPI GCANNNNNTGC 1 cut(s) 1103
BstAUI TGTACA 1 cut(s) 198
BstBAI YACGTR 1 cut(s) 626
BstC8I GCNNGC 1 cut(s) 771
BstDEI CTNAG 3 cut(s) 306, 839, 861
BstDSI CCRYGG 1 cut(s) 1107
BstF5I GGATG 3 cut(s) 463, 877, 1025
BstFNI CGCG 2 cut(s) 317, 918
BstHHI GCGC 1 cut(s) 548
BstKTI GATC 3 cut(s) 191, 334, 784
BstMAI GTCTC 4 cut(s) 263, 350, 555, 829
BstMBI GATC 3 cut(s) 188, 331, 781
BstMCI CGRYCG 2 cut(s) 191, 238
BstMWI GCNNNNNNNGC 4 cut(s) 347, 775, 784, 1103
BstSCI CCNGG 2 cut(s) 153, 414
BstSFI CTRYAG 1 cut(s) 996
BstSLI GKGCMC 1 cut(s) 678
BstUI CGCG 2 cut(s) 317, 918
BstV1I GCAGC 2 cut(s) 982, 1088
BstV2I GAAGAC 3 cut(s) 116, 171, 694
BstXI CCANNNNNNTGG 1 cut(s) 743
Bsu15I ATCGAT 1 cut(s) 1046
BsuRI GGCC 2 cut(s) 735, 828
BsuTUI ATCGAT 1 cut(s) 1046
BtgI CCRYGG 1 cut(s) 1107
BtrI CACGTC 1 cut(s) 766
BtsCI GGATG 3 cut(s) 463, 877, 1025
BtsIMutI CAGTG 2 cut(s) 208, 904
BtuMI TCGCGA 1 cut(s) 317
Cac8I GCNNGC 1 cut(s) 771
CciI TCATGA 1 cut(s) 265
CfoI GCGC 1 cut(s) 548
Cfr10I RCCGGY 1 cut(s) 215
Cfr13I GGNCC 2 cut(s) 227, 584
ClaI ATCGAT 1 cut(s) 1046
Csp6I GTAC 3 cut(s) 199, 207, 232
CspCI CAANNNNNGTGG 2 cut(s) 553, 588
CviAII CATG 6 cut(s) 242, 266, 495, 551, 978, 1024
CviQI GTAC 3 cut(s) 199, 207, 232
DdeI CTNAG 3 cut(s) 306, 839, 861
DpnI GATC 3 cut(s) 190, 333, 783
DpnII GATC 3 cut(s) 188, 331, 781
DraI TTTAAA 1 cut(s) 184
EaeI YGGCCR 1 cut(s) 733
Eam1104I CTCTTC 3 cut(s) 61, 510, 1053
EarI CTCTTC 3 cut(s) 61, 510, 1053
EciI GGCGGA 1 cut(s) 137
Eco130I CCWWGG 1 cut(s) 958
Eco47I GGWCC 2 cut(s) 227, 584
Eco57I CTGAAG 2 cut(s) 315, 688
Eco72I CACGTG 1 cut(s) 626
Eco88I CYCGRG 1 cut(s) 845
EcoT14I CCWWGG 1 cut(s) 958
EcoT22I ATGCAT 1 cut(s) 1025
ErhI CCWWGG 1 cut(s) 958
Esp3I CGTCTC 1 cut(s) 350
FaeI CATG 6 cut(s) 245, 269, 498, 554, 981, 1027
FalI AAGNNNNNCTT 4 cut(s) 156, 188, 462, 494
FaqI GGGAC 1 cut(s) 137
FatI CATG 6 cut(s) 241, 265, 494, 550, 977, 1023
FbaI TGATCA 1 cut(s) 781
Fnu4HI GCNGC 2 cut(s) 996, 1077
FokI GGATG 3 cut(s) 470, 884, 1032
Fsp4HI GCNGC 2 cut(s) 996, 1077
FspBI CTAG 1 cut(s) 89
GlaI GCGC 1 cut(s) 547
GluI GCNGC 2 cut(s) 996, 1077
HaeIII GGCC 2 cut(s) 735, 828
HapII CCGG 3 cut(s) 155, 216, 416
HhaI GCGC 1 cut(s) 548
Hin1II CATG 6 cut(s) 245, 269, 498, 554, 981, 1027
Hin6I GCGC 1 cut(s) 546
HinP1I GCGC 1 cut(s) 546
HincII GTYRAC 2 cut(s) 145, 522
HindII GTYRAC 2 cut(s) 145, 522
HindIII AAGCTT 1 cut(s) 362
HinfI GANTC 7 cut(s) 34, 262, 389, 439, 508, 713, 754
HpaI GTTAAC 1 cut(s) 145
HpaII CCGG 3 cut(s) 155, 216, 416
HphI GGTGA 2 cut(s) 398, 863
Hpy166II GTNNAC 6 cut(s) 145, 522, 584, 623, 629, 942
Hpy188I TCNGA 4 cut(s) 163, 193, 394, 637
Hpy188III TCNNGA 7 cut(s) 266, 316, 443, 512, 758, 822, 1009
Hpy8I GTNNAC 6 cut(s) 145, 522, 584, 623, 629, 942
HpyAV CCTTC 3 cut(s) 290, 1010, 1122
HpyCH4III ACNGT 5 cut(s) 203, 236, 370, 899, 1102
HpyCH4IV ACGT 3 cut(s) 141, 625, 765
HpyCH4V TGCA 5 cut(s) 773, 794, 965, 998, 1023
HpyF10VI GCNNNNNNNGC 4 cut(s) 347, 775, 784, 1103
HpyF3I CTNAG 3 cut(s) 306, 839, 861
HpySE526I ACGT 3 cut(s) 141, 625, 765
Hsp92II CATG 6 cut(s) 245, 269, 498, 554, 981, 1027
HspAI GCGC 1 cut(s) 546
Ksp22I TGATCA 1 cut(s) 781
KspAI GTTAAC 1 cut(s) 145
Kzo9I GATC 3 cut(s) 188, 331, 781
LmnI GCTCC 2 cut(s) 928, 1073
Lsp1109I GCAGC 2 cut(s) 982, 1088
LweI GCATC 2 cut(s) 448, 1010
MaeI CTAG 1 cut(s) 89
MaeII ACGT 3 cut(s) 141, 625, 765
MaeIII GTNAC 2 cut(s) 254, 751
MalI GATC 3 cut(s) 190, 333, 783
MboI GATC 3 cut(s) 188, 331, 781
MhlI GDGCHC 1 cut(s) 678
MlsI TGGCCA 1 cut(s) 735
MluCI AATT 2 cut(s) 11, 691
MluNI TGGCCA 1 cut(s) 735
MlyI GAGTC 2 cut(s) 271, 748
MnlI CCTC 9 cut(s) 96, 291, 315, 818, 841, 847, 862, 941, 1054
Mox20I TGGCCA 1 cut(s) 735
Mph1103I ATGCAT 1 cut(s) 1025
MroXI GAANNNNTTC 5 cut(s) 300, 727, 807, 938, 1062
MscI TGGCCA 1 cut(s) 735
MseI TTAA 4 cut(s) 144, 183, 327, 1138
MslI CAYNNNNRTG 4 cut(s) 452, 669, 681, 741
Msp20I TGGCCA 1 cut(s) 735
MspA1I CMGCKG 2 cut(s) 719, 1091
MspI CCGG 3 cut(s) 155, 216, 416
MspR9I CCNGG 2 cut(s) 155, 416
MvnI CGCG 2 cut(s) 317, 918
MwoI GCNNNNNNNGC 4 cut(s) 347, 775, 784, 1103
NciI CCSGG 2 cut(s) 155, 416
NdeII GATC 3 cut(s) 188, 331, 781
NlaIII CATG 6 cut(s) 245, 269, 498, 554, 981, 1027
NlaIV GGNNCC 2 cut(s) 220, 529
NmeAIII GCCGAG 1 cut(s) 584
NmuCI GTSAC 1 cut(s) 751
NruI TCGCGA 1 cut(s) 317
NsiI ATGCAT 1 cut(s) 1025
OliI CACNNNNGTG 1 cut(s) 452
PagI TCATGA 1 cut(s) 265
PcsI WCGNNNNNNNCGW 1 cut(s) 219
PdmI GAANNNNTTC 5 cut(s) 300, 727, 807, 938, 1062
PfeI GAWTC 5 cut(s) 34, 389, 439, 508, 713
Pfl23II CGTACG 1 cut(s) 231
PfoI TCCNGGA 1 cut(s) 414
PkrI GCNGC 2 cut(s) 997, 1078
Ple19I CGATCG 1 cut(s) 191
PleI GAGTC 2 cut(s) 270, 748
PmaCI CACGTG 1 cut(s) 626
PmlI CACGTG 1 cut(s) 626
PpsI GAGTC 2 cut(s) 270, 748
Ppu21I YACGTR 1 cut(s) 626
Psp1406I AACGTT 1 cut(s) 141
PspCI CACGTG 1 cut(s) 626
PspLI CGTACG 1 cut(s) 231
PspN4I GGNNCC 2 cut(s) 220, 529
PspPI GGNCC 2 cut(s) 227, 584
PstI CTGCAG 1 cut(s) 1000
PvuI CGATCG 1 cut(s) 191
PvuII CAGCTG 2 cut(s) 719, 1091
RruI TCGCGA 1 cut(s) 317
RsaI GTAC 3 cut(s) 200, 208, 233
RsaNI GTAC 3 cut(s) 199, 207, 232
RseI CAYNNNNRTG 4 cut(s) 452, 669, 681, 741
SaqAI TTAA 4 cut(s) 144, 183, 327, 1138
SatI GCNGC 2 cut(s) 996, 1077
Sau3AI GATC 3 cut(s) 188, 331, 781
Sau96I GGNCC 2 cut(s) 227, 584
SchI GAGTC 2 cut(s) 271, 748
ScrFI CCNGG 2 cut(s) 155, 416
SduI GDGCHC 1 cut(s) 678
SfaNI GCATC 2 cut(s) 448, 1010
SfcI CTRYAG 1 cut(s) 996
SinI GGWCC 2 cut(s) 227, 584
SmiMI CAYNNNNRTG 4 cut(s) 452, 669, 681, 741
Sse9I AATT 2 cut(s) 11, 691
SsiI CCGC 1 cut(s) 122
SspMI CTAG 1 cut(s) 89
StyD4I CCNGG 2 cut(s) 153, 414
StyI CCWWGG 1 cut(s) 958
TaaI ACNGT 5 cut(s) 203, 236, 370, 899, 1102
TaiI ACGT 3 cut(s) 144, 628, 768
TaqI TCGA 3 cut(s) 28, 606, 1046
TasI AATT 2 cut(s) 11, 691
TatI WGTACW 1 cut(s) 198
TfiI GAWTC 5 cut(s) 34, 389, 439, 508, 713
Tru1I TTAA 4 cut(s) 144, 183, 327, 1138
Tru9I TTAA 4 cut(s) 144, 183, 327, 1138
TscAI CASTG 2 cut(s) 208, 904
TseFI GTSAC 1 cut(s) 751
TseI GCWGC 2 cut(s) 995, 1076
Tsp45I GTSAC 1 cut(s) 751
TspDTI ATGAA 2 cut(s) 966, 1040
TspGWI ACGGA 5 cut(s) 192, 211, 219, 906, 984
TspRI CASTG 2 cut(s) 208, 904
VpaK11BI GGWCC 2 cut(s) 227, 584
XapI RAATTY 2 cut(s) 11, 691
XmnI GAANNNNTTC 5 cut(s) 300, 727, 807, 938, 1062
XspI CTAG 1 cut(s) 89
Zsp2I ATGCAT 1 cut(s) 1025
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.