MD09G1024100.v1.1

Altered inheritance of mitochondria protein 32-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0001205
Physical Location & Seq
Reverse (-)
1439903 .. 1445343
5441 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1024100.v1.1.491

Sequence Viewer

Length: 1689 bp
ATGTCGAAAATGAAACACTTGCAACTGAAAGACTGCCGCAAACTCAAAGATATTCCAAACTTGGAGAACTCATTGGACTACATGGATTCAATTTATATGGAAGGGTGTACCAGTCTCACTGATACTTTTAAGGAGAACCTCCAAACGAAAAATGCATTTGGTGGAATTTTTCTCTCTAGAAATGATATTCCGAAGCGGTTAGCCTATGTCGCGAGAGAGGATGAAACTGTCAAATTTAAAGTGCCGCCATGTATTGATTATATAGGAGGGTTGGCTTTGGGCATCGTTTATTCTTCGGACAACTCCGACTCTAGTGGGTTTCTAACCATTGATGTTGTTAATCGTACCCAGCAAACTAATTTTCGTATCTGGCCAATTGAGGCCACCGTAACTGCTTCCCATGAATATTATCTTTGGTTGGGAAATCTTTCAAACAAGAAGCTCAATTTGAAAGGCGGCGACATGGTTCTTGTACAAGCACAATTTTATGGACTAGATAATACAATTATTAAGGTGAACAAAACTGGAGTGGATATTGTAAAATGGGATTTGTCCGATGATCGTACTAATTGGGACAACTATGAGACTATGTCATATGAGTCTGATGAAGACACTACTGTGTCATATGAGTCTGATGAAGACACTGATGACGATACACTGTCATACTATCGCCACGTTTTCCTCTGCTACAAGACCCTCGAGGCCTGGCCATGGCGCATCAAAGGCTATGAGTCCGATCCACTCCCTAAAATCTTCGCATCTGCTCTCAAAGCTCGCAAGAACGACATCACCTTTAAGACGTTGTTGACAGTAATTGAAGGACGCGAAGGAACTGAGTTTTCCGACGGCGATGTGTTGATTTTCCCACAAATGATCAAATACAGGGGCTTGAAAGAGTCGGATGTGGACAGCTTTGTTGATGATGTCCTTGTGAATGATAGACCATGGGCTTCGAGAGTGCAAGAGCCGTTGACTGGCCCCCATGTATTTGCCCTTATATTTGTATACACACATATGAGTCAAGAGGAATGGAATCGTGATAGCGCACGTGTTCGCATTGATAAGTTGAAAGAAGAGTTTGAGCTGCGAGGATTGACGAATCAGGTATTTGTTACTGCTTGCTTTCACCTTGAAGGCCACAACTATGCTGGAATATTCACGACCTACGACCCAGATGGAAGCATGACATACCATTGGTATCCATTCGTCAGTCCTGGTTATGTGCCGGAATTGCTTGATGAGCATATTGGGAAGGGAGAGATTATAGAACGACGTTGGAGGGGCCAAATGGGAGCATCTTCTGATGAAGCTGAAGAAATTAATGACCGGGAGCTTCCAAATGGAGAAGAAAATAAGAAAATCGAGGATAAGCCACAAGAAAATGGCAATCAGATTCACCAAGAAAAGGGCAATCAAATTGAGAATAATGAGAACTTTTCAGGCTATTGCCAGAGTGCTAATAGTGATGGATTTACATACTGCAAAGAAGTAAGTTTGGAGGAGGACGGTGGAAGTGAGGATAAAAAGCCGAAAGAAACCACAGAGTCGTGTGCTAGGAAGGATGGCTTACAGAAGCTATCAAGTTTGATAGGGAACTGGGAGCAAAGCGACGTTCTCGCAGCTGCTGCCGTGGTTGGAGCAGTGGCAACAGTGGCTGTGGCCTACAGCTTTTACAGAAGGTCAGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Suc_Fer-like

Protein Analysis

563

Amino Acids

64.02

Weight (kDa)

4.78

Isoelectric Point (pI)

38.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Suc_Fer-like PF06999 221 - 428 5e-18 Sucrase/ferredoxin-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 698
AccI GTMKAC 1 cut(s) 1005
AccII CGCG 2 cut(s) 212, 825
AciI CCGC 4 cut(s) 37, 196, 245, 456
AclWI GGATC 1 cut(s) 731
AcoI YGGCCR 2 cut(s) 371, 707
AcsI RAATTY 2 cut(s) 165, 233
AcuI CTGAAG 1 cut(s) 1332
AcvI CACGTG 1 cut(s) 1049
AfaI GTAC 4 cut(s) 109, 346, 474, 565
AfiI CCNNNNNNNGG 3 cut(s) 711, 974, 1405
AflIII ACRYGT 1 cut(s) 1048
AgsI TTSAA 7 cut(s) 90, 432, 451, 818, 892, 1069, 1133
AjnI CCWGG 2 cut(s) 704, 1213
AleI CACNNNNGTG 1 cut(s) 617
AluBI AGCT 9 cut(s) 442, 773, 912, 1084, 1310, 1333, 1576, 1622, 1668
AluI AGCT 9 cut(s) 442, 773, 912, 1084, 1310, 1333, 1576, 1622, 1668
Alw26I GTCTC 2 cut(s) 119, 578
AlwI GGATC 1 cut(s) 731
AlwNI CAGNNNCTG 2 cut(s) 1625, 1655
Ama87I CYCGRG 1 cut(s) 698
AoxI GGCC 8 cut(s) 371, 381, 702, 707, 976, 1135, 1282, 1659
ApeKI GCWGC 4 cut(s) 1084, 1619, 1622, 1625
ApoI RAATTY 2 cut(s) 165, 233
AseI ATTAAT 1 cut(s) 1320
Asp700I GAANNNNTTC 1 cut(s) 427
AspLEI GCGC 2 cut(s) 717, 1046
AspS9I GGNCC 2 cut(s) 977, 1282
AsuC2I CCSGG 1 cut(s) 1328
AsuHPI GGTGA 4 cut(s) 526, 781, 1118, 1388
AvaI CYCGRG 1 cut(s) 698
BalI TGGCCA 2 cut(s) 373, 709
BbrPI CACGTG 1 cut(s) 1049
BbsI GAAGAC 2 cut(s) 615, 645
BbvI GCAGC 4 cut(s) 1071, 1609, 1612, 1631
BccI CCATC 3 cut(s) 1169, 1460, 1556
BceAI ACGGC 3 cut(s) 862, 952, 1613
BciT130I CCWGG 2 cut(s) 706, 1215
BciVI GTATCC 1 cut(s) 1209
BclI TGATCA 1 cut(s) 873
BcnI CCSGG 1 cut(s) 1328
BcoDI GTCTC 2 cut(s) 119, 578
BfaI CTAG 4 cut(s) 177, 312, 494, 1554
BfmI CTRYAG 1 cut(s) 1663
BfuI GTATCC 1 cut(s) 1209
BisI GCNGC 7 cut(s) 37, 245, 457, 1085, 1620, 1623, 1626
BlsI GCNGC 7 cut(s) 38, 246, 458, 1086, 1621, 1624, 1627
Bme1390I CCNGG 3 cut(s) 706, 1215, 1328
BmeT110I CYCGRG 1 cut(s) 698
BmgT120I GGNCC 2 cut(s) 977, 1282
BmiI GGNNCC 2 cut(s) 979, 1283
BmrFI CCNGG 3 cut(s) 706, 1215, 1328
BmrI ACTGGG 1 cut(s) 1606
BmsI GCATC 4 cut(s) 291, 726, 767, 1304
BmuI ACTGGG 1 cut(s) 1606
BpiI GAAGAC 2 cut(s) 615, 645
BpmI CTGGAG 1 cut(s) 546
BpuMI CCSGG 1 cut(s) 1328
BsaAI YACGTR 1 cut(s) 1049
BsaJI CCNNGG 3 cut(s) 710, 944, 1629
Bsc4I CCNNNNNNNGG 3 cut(s) 711, 974, 1405
Bse1I ACTGG 4 cut(s) 111, 529, 979, 1601
BseBI CCWGG 2 cut(s) 706, 1215
BseDI CCNNGG 3 cut(s) 710, 944, 1629
BseGI GGATG 3 cut(s) 226, 907, 1567
BseLI CCNNNNNNNGG 3 cut(s) 711, 974, 1405
BseMII CTCAG 1 cut(s) 825
BseNI ACTGG 4 cut(s) 111, 529, 979, 1601
BseRI GAGGAG 1 cut(s) 1514
BseXI GCAGC 4 cut(s) 1071, 1609, 1612, 1631
BseYI CCCAGC 1 cut(s) 348
Bsh1236I CGCG 2 cut(s) 212, 825
BshFI GGCC 8 cut(s) 373, 383, 704, 709, 978, 1137, 1284, 1661
BsiHKCI CYCGRG 1 cut(s) 698
BsiSI CCGG 2 cut(s) 1226, 1327
BslFI GGGAC 1 cut(s) 587
BslI CCNNNNNNNGG 3 cut(s) 711, 974, 1405
BsmAI GTCTC 2 cut(s) 119, 578
BsmFI GGGAC 1 cut(s) 587
BsnI GGCC 8 cut(s) 373, 383, 704, 709, 978, 1137, 1284, 1661
BsoBI CYCGRG 1 cut(s) 698
Bsp1407I TGTACA 1 cut(s) 472
Bsp143I GATC 3 cut(s) 559, 736, 873
Bsp19I CCATGG 2 cut(s) 710, 944
Bsp68I TCGCGA 1 cut(s) 212
BspACI CCGC 4 cut(s) 37, 196, 245, 456
BspANI GGCC 8 cut(s) 373, 383, 704, 709, 978, 1137, 1284, 1661
BspCNI CTCAG 1 cut(s) 826
BspFNI CGCG 2 cut(s) 212, 825
BspLI GGNNCC 2 cut(s) 979, 1283
BspPI GGATC 1 cut(s) 731
BsrGI TGTACA 1 cut(s) 472
BsrI ACTGG 4 cut(s) 111, 529, 979, 1601
BssECI CCNNGG 3 cut(s) 710, 944, 1629
BssMI GATC 3 cut(s) 559, 736, 873
BssNAI GTATAC 1 cut(s) 1006
BssT1I CCWWGG 2 cut(s) 710, 944
Bst1107I GTATAC 1 cut(s) 1006
Bst2UI CCWGG 2 cut(s) 706, 1215
Bst4CI ACNGT 7 cut(s) 229, 388, 619, 660, 811, 1508, 1651
Bst6I CTCTTC 1 cut(s) 1068
BstAPI GCANNNNNTGC 1 cut(s) 1625
BstAUI TGTACA 1 cut(s) 472
BstBAI YACGTR 1 cut(s) 1049
BstC8I GCNNGC 2 cut(s) 775, 1120
BstDEI CTNAG 1 cut(s) 834
BstDSI CCRYGG 3 cut(s) 710, 944, 1629
BstF5I GGATG 3 cut(s) 226, 907, 1567
BstFNI CGCG 2 cut(s) 212, 825
BstHHI GCGC 2 cut(s) 717, 1046
BstKTI GATC 3 cut(s) 562, 739, 876
BstMAI GTCTC 2 cut(s) 119, 578
BstMBI GATC 3 cut(s) 559, 736, 873
BstMWI GCNNNNNNNGC 7 cut(s) 209, 723, 770, 1231, 1240, 1625, 1652
BstNI CCWGG 2 cut(s) 706, 1215
BstSCI CCNGG 3 cut(s) 704, 1213, 1326
BstSFI CTRYAG 1 cut(s) 1663
BstUI CGCG 2 cut(s) 212, 825
BstV1I GCAGC 4 cut(s) 1071, 1609, 1612, 1631
BstV2I GAAGAC 2 cut(s) 615, 645
BstZ17I GTATAC 1 cut(s) 1006
BsuI GTATCC 1 cut(s) 1209
BsuRI GGCC 8 cut(s) 373, 383, 704, 709, 978, 1137, 1284, 1661
BtgI CCRYGG 3 cut(s) 710, 944, 1629
BtgZI GCGATG 1 cut(s) 864
BtsCI GGATG 3 cut(s) 226, 907, 1567
BtsI GCAGTG 1 cut(s) 1647
BtsIMutI CAGTG 5 cut(s) 117, 642, 656, 1647, 1656
BtuMI TCGCGA 1 cut(s) 212
Cac8I GCNNGC 2 cut(s) 775, 1120
CaiI CAGNNNCTG 2 cut(s) 1625, 1655
CfoI GCGC 2 cut(s) 717, 1046
Cfr13I GGNCC 2 cut(s) 977, 1282
CseI GACGC 1 cut(s) 831
Csp6I GTAC 4 cut(s) 108, 345, 473, 564
CviAII CATG 8 cut(s) 82, 249, 401, 463, 711, 945, 983, 1183
CviQI GTAC 4 cut(s) 108, 345, 473, 564
DdeI CTNAG 1 cut(s) 834
DpnI GATC 3 cut(s) 561, 738, 875
DpnII GATC 3 cut(s) 559, 736, 873
DraI TTTAAA 1 cut(s) 238
EaeI YGGCCR 2 cut(s) 371, 707
Eam1104I CTCTTC 1 cut(s) 1068
EarI CTCTTC 1 cut(s) 1068
Eco130I CCWWGG 2 cut(s) 710, 944
Eco147I AGGCCT 1 cut(s) 704
Eco57I CTGAAG 1 cut(s) 1332
Eco72I CACGTG 1 cut(s) 1049
Eco88I CYCGRG 1 cut(s) 698
EcoRII CCWGG 2 cut(s) 704, 1213
EcoT14I CCWWGG 2 cut(s) 710, 944
EcoT22I ATGCAT 1 cut(s) 157
ErhI CCWWGG 2 cut(s) 710, 944
FaeI CATG 8 cut(s) 85, 252, 404, 466, 714, 948, 986, 1186
FalI AAGNNNNNCTT 2 cut(s) 1550, 1582
FaqI GGGAC 1 cut(s) 587
FatI CATG 8 cut(s) 81, 248, 400, 462, 710, 944, 982, 1182
FauNDI CATATG 3 cut(s) 595, 625, 1014
FbaI TGATCA 1 cut(s) 873
FblI GTMKAC 1 cut(s) 1005
Fnu4HI GCNGC 7 cut(s) 37, 245, 457, 1085, 1620, 1623, 1626
FokI GGATG 3 cut(s) 233, 914, 1574
Fsp4HI GCNGC 7 cut(s) 37, 245, 457, 1085, 1620, 1623, 1626
FspBI CTAG 4 cut(s) 177, 312, 494, 1554
GlaI GCGC 2 cut(s) 716, 1045
GluI GCNGC 7 cut(s) 37, 245, 457, 1085, 1620, 1623, 1626
GsaI CCCAGC 1 cut(s) 352
GsuI CTGGAG 1 cut(s) 546
HaeIII GGCC 8 cut(s) 373, 383, 704, 709, 978, 1137, 1284, 1661
HapII CCGG 2 cut(s) 1226, 1327
HgaI GACGC 1 cut(s) 831
HhaI GCGC 2 cut(s) 717, 1046
Hin1II CATG 8 cut(s) 85, 252, 404, 466, 714, 948, 986, 1186
Hin6I GCGC 2 cut(s) 715, 1044
HinP1I GCGC 2 cut(s) 715, 1044
HincII GTYRAC 2 cut(s) 807, 972
HindII GTYRAC 2 cut(s) 807, 972
HpaII CCGG 2 cut(s) 1226, 1327
HphI GGTGA 4 cut(s) 526, 781, 1118, 1388
Hpy166II GTNNAC 6 cut(s) 108, 517, 807, 907, 972, 1006
Hpy188III TCNNGA 6 cut(s) 177, 211, 954, 1022, 1037, 1159
Hpy8I GTNNAC 6 cut(s) 108, 517, 807, 907, 972, 1006
Hpy99I CGWCG 3 cut(s) 848, 1275, 1613
HpyAV CCTTC 7 cut(s) 95, 812, 821, 1127, 1246, 1552, 1671
HpyCH4III ACNGT 7 cut(s) 229, 388, 619, 660, 811, 1508, 1651
HpyCH4IV ACGT 5 cut(s) 675, 800, 1048, 1273, 1611
HpyCH4V TGCA 4 cut(s) 22, 155, 961, 1482
HpyF10VI GCNNNNNNNGC 7 cut(s) 209, 723, 770, 1231, 1240, 1625, 1652
HpyF3I CTNAG 1 cut(s) 834
HpySE526I ACGT 5 cut(s) 675, 800, 1048, 1273, 1611
Hsp92II CATG 8 cut(s) 85, 252, 404, 466, 714, 948, 986, 1186
HspAI GCGC 2 cut(s) 715, 1044
Ksp22I TGATCA 1 cut(s) 873
Kzo9I GATC 3 cut(s) 559, 736, 873
LmnI GCTCC 4 cut(s) 1292, 1330, 1600, 1637
Lsp1109I GCAGC 4 cut(s) 1071, 1609, 1612, 1631
LweI GCATC 4 cut(s) 291, 726, 767, 1304
MaeI CTAG 4 cut(s) 177, 312, 494, 1554
MaeII ACGT 5 cut(s) 675, 800, 1048, 1273, 1611
MaeIII GTNAC 2 cut(s) 388, 1111
MalI GATC 3 cut(s) 561, 738, 875
MboI GATC 3 cut(s) 559, 736, 873
MboII GAAGA 8 cut(s) 285, 620, 650, 745, 1085, 1290, 1325, 1358
MfeI CAATTG 1 cut(s) 375
MlsI TGGCCA 2 cut(s) 373, 709
MluNI TGGCCA 2 cut(s) 373, 709
MlyI GAGTC 7 cut(s) 302, 608, 638, 740, 905, 1027, 1553
MmeI TCCRAC 5 cut(s) 330, 867, 879, 1256, 1615
Mox20I TGGCCA 2 cut(s) 373, 709
Mph1103I ATGCAT 1 cut(s) 157
MroXI GAANNNNTTC 1 cut(s) 427
MscI TGGCCA 2 cut(s) 373, 709
MseI TTAA 6 cut(s) 129, 237, 339, 510, 795, 1320
MslI CAYNNNNRTG 3 cut(s) 617, 1013, 1143
Msp20I TGGCCA 2 cut(s) 373, 709
MspA1I CMGCKG 1 cut(s) 1622
MspI CCGG 2 cut(s) 1226, 1327
MspR9I CCNGG 3 cut(s) 706, 1215, 1328
MunI CAATTG 1 cut(s) 375
MvaI CCWGG 2 cut(s) 706, 1215
MvnI CGCG 2 cut(s) 212, 825
MwoI GCNNNNNNNGC 7 cut(s) 209, 723, 770, 1231, 1240, 1625, 1652
NciI CCSGG 1 cut(s) 1328
NcoI CCATGG 2 cut(s) 710, 944
NdeI CATATG 3 cut(s) 595, 625, 1014
NdeII GATC 3 cut(s) 559, 736, 873
NlaIII CATG 8 cut(s) 85, 252, 404, 466, 714, 948, 986, 1186
NlaIV GGNNCC 2 cut(s) 979, 1283
NruI TCGCGA 1 cut(s) 212
NsiI ATGCAT 1 cut(s) 157
OliI CACNNNNGTG 1 cut(s) 617
PaeR7I CTCGAG 1 cut(s) 698
PceI AGGCCT 1 cut(s) 704
PdmI GAANNNNTTC 1 cut(s) 427
PfeI GAWTC 4 cut(s) 86, 1033, 1099, 1393
PflFI GACNNNGTC 1 cut(s) 589
PkrI GCNGC 7 cut(s) 38, 246, 458, 1086, 1621, 1624, 1627
PleI GAGTC 7 cut(s) 302, 607, 637, 739, 904, 1026, 1552
PmaCI CACGTG 1 cut(s) 1049
PmlI CACGTG 1 cut(s) 1049
PpsI GAGTC 7 cut(s) 302, 607, 637, 739, 904, 1026, 1552
Ppu21I YACGTR 1 cut(s) 1049
PshBI ATTAAT 1 cut(s) 1320
Psp6I CCWGG 2 cut(s) 704, 1213
PspCI CACGTG 1 cut(s) 1049
PspFI CCCAGC 1 cut(s) 348
PspGI CCWGG 2 cut(s) 704, 1213
PspN4I GGNNCC 2 cut(s) 979, 1283
PspPI GGNCC 2 cut(s) 977, 1282
PspXI VCTCGAGB 1 cut(s) 698
PstNI CAGNNNCTG 2 cut(s) 1625, 1655
PsyI GACNNNGTC 1 cut(s) 589
PvuII CAGCTG 1 cut(s) 1622
RruI TCGCGA 1 cut(s) 212
RsaI GTAC 4 cut(s) 109, 346, 474, 565
RsaNI GTAC 4 cut(s) 108, 345, 473, 564
RseI CAYNNNNRTG 3 cut(s) 617, 1013, 1143
SaqAI TTAA 6 cut(s) 129, 237, 339, 510, 795, 1320
SatI GCNGC 7 cut(s) 37, 245, 457, 1085, 1620, 1623, 1626
Sau3AI GATC 3 cut(s) 559, 736, 873
Sau96I GGNCC 2 cut(s) 977, 1282
SchI GAGTC 7 cut(s) 302, 608, 638, 740, 905, 1027, 1553
ScrFI CCNGG 3 cut(s) 706, 1215, 1328
SfaNI GCATC 4 cut(s) 291, 726, 767, 1304
SfcI CTRYAG 1 cut(s) 1663
Sfr274I CTCGAG 1 cut(s) 698
SlaI CTCGAG 1 cut(s) 698
SmiMI CAYNNNNRTG 3 cut(s) 617, 1013, 1143
SmlI CTYRAG 1 cut(s) 698
SmoI CTYRAG 1 cut(s) 698
SseBI AGGCCT 1 cut(s) 704
SsiI CCGC 4 cut(s) 37, 196, 245, 456
SspI AATATT 2 cut(s) 407, 1155
SspMI CTAG 4 cut(s) 177, 312, 494, 1554
StuI AGGCCT 1 cut(s) 704
StyD4I CCNGG 3 cut(s) 704, 1213, 1326
StyI CCWWGG 2 cut(s) 710, 944
TaaI ACNGT 7 cut(s) 229, 388, 619, 660, 811, 1508, 1651
TaiI ACGT 5 cut(s) 678, 803, 1051, 1276, 1614
TaqI TCGA 4 cut(s) 5, 699, 953, 1362
TatI WGTACW 1 cut(s) 472
TauI GCSGC 3 cut(s) 39, 247, 459
TfiI GAWTC 4 cut(s) 86, 1033, 1099, 1393
Tru1I TTAA 6 cut(s) 129, 237, 339, 510, 795, 1320
Tru9I TTAA 6 cut(s) 129, 237, 339, 510, 795, 1320
TscAI CASTG 5 cut(s) 124, 649, 663, 1647, 1656
TseI GCWGC 4 cut(s) 1084, 1619, 1622, 1625
TspDTI ATGAA 6 cut(s) 26, 237, 417, 621, 651, 1320
TspRI CASTG 5 cut(s) 124, 649, 663, 1647, 1656
Tth111I GACNNNGTC 1 cut(s) 589
VspI ATTAAT 1 cut(s) 1320
XapI RAATTY 2 cut(s) 165, 233
XbaI TCTAGA 1 cut(s) 176
XcmI CCANNNNNNNNNTGG 1 cut(s) 1145
XhoI CTCGAG 1 cut(s) 698
XmiI GTMKAC 1 cut(s) 1005
XmnI GAANNNNTTC 1 cut(s) 427
XspI CTAG 4 cut(s) 177, 312, 494, 1554
Zsp2I ATGCAT 1 cut(s) 157
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.